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Chromatography, Ion ExchangeAnion Exchange ResinsChromatography, GelChromatography, High Pressure LiquidAnionsIon Exchange ResinsChromatography, AffinityMolecular WeightIon ExchangeElectrophoresis, Polyacrylamide GelChromatographyMolecular Sequence DataKineticsAmino Acid SequenceHydrogen-Ion ConcentrationAmino AcidsSubstrate SpecificityCattleCarbohydratesCarbohydrate SequenceOligosaccharidesChromatography, LiquidMass SpectrometryChromatography, GasChromatography, Thin LayerCarbohydrate ConformationChromatography, DEAE-CelluloseSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationRecombinant ProteinsPeptide FragmentsAnion Exchange Protein 1, ErythrocyteEscherichia coliHydrolysisAnion Transport ProteinsChloridesGlycoproteins4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidIsoenzymesBase SequenceCytosolChloride-Bicarbonate Antiporters4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidSulfatesCloning, MolecularIsoelectric PointAntiportersChromatography, AgaroseGlycosylationBlotting, WesternBicarbonatesIsoelectric FocusingSLC4A ProteinsEnzyme ActivationTemperatureCells, CulturedRabbitsChemical FractionationTrypsinGas Chromatography-Mass SpectrometrySpectrophotometry, UltravioletProtein BindingMagnetic Resonance SpectroscopyErythrocytesMacromolecular SubstancesDeuterium Exchange MeasurementCarrier ProteinsPeptidesCation Exchange ResinsSpectrometry, Mass, Electrospray IonizationLiverSodiumPulmonary Gas ExchangeCell MembraneBinding SitesAmmonium SulfateBiological TransportExchange Transfusion, Whole BloodChemistryCell LineProtein ConformationChemical PhenomenaSequence Homology, Amino AcidSister Chromatid ExchangeCationsImmunodiffusionSolubilityIon ChannelsCalciumMethodsDrug StabilityProteinsEnzyme StabilityIon TransportTandem Mass SpectrometryGlycosaminoglycansBromidesGlycoside HydrolasesMolecular StructurePolysaccharidesIndicators and Reagents
Affinity chromatographyCationStrongly basic anion exchange resinAmperometric DetectionHPLCDetectionResinMass spectrometryConductivitySize-exclusion chroInorganicSeparationsProteinsCarbohydratesLigandAqueousSeven common anionsMembraneProteinDisaccharidesResinsMoleculesInositolElutionLiquidColumnAnalysisStrongElectronCopperEnzymeProcessBreakthroughAnalytesNegative ionPhaseMethodMethodsBasicArticle
Affinity chromatography10
  • Ion exchange chromatography can be used either for a separate purification step or for a second polishing purification step after affinity chromatography purification as an orthogonal chemistry to obtain a highly pure final product. (biotage.com)
  • The ability of protein A affinity chromatography to achieve 95% purity in one simple step was the runway that got recombinant immunotherapy off the ground and made it available to millions. (bioprocessintl.com)
  • The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. (brighton.ac.uk)
  • Miniaturized affinity chromatography: A powerful technique for the isolation of high affinity GAGs sequences prior to their identification by MALDI-TOF MS. Analytica Chimica Acta , 2023, 1277, pp.341656. (isa-lyon.fr)
  • Optimization of the Preparation of Hydrophilic Poly(DHPMA-co-MBA) Monolithic Capillary Columns: A New Support for Affinity Chromatography. (isa-lyon.fr)
  • Deciphering dynamic combinatorial libraries of glycoclusters with miniaturized weak affinity chromatography coupled with mass spectrometry (nano-FAC-MS). Analytica Chimica Acta , 2023, 1261, pp.341227. (isa-lyon.fr)
  • Two Original Experimental Setups for Staircase Frontal Affinity Chromatography at the Miniaturized Scale. (isa-lyon.fr)
  • Miniaturized weak affinity chromatography for ligand identification of nanodiscs-embedded G-protein coupled receptors. (isa-lyon.fr)
  • Affinity Chromatography: A Powerful Tool in Drug Discovery for Investigating Ligand/membrane Protein Interactions. (isa-lyon.fr)
  • Monolith weak affinity chromatography for μg-protein-ligand interaction study. (isa-lyon.fr)
Cation9
  • These charged groups are referred to as exchangers like cation and anion exchangers. (wikipedia.org)
  • The stability of the protein at values above or below the pI, will determine if an anion exchange column or cation exchange column should be used. (wikipedia.org)
  • If it is stable at pH values below the pI, the cation exchange column be used. (wikipedia.org)
  • For inorganic anion/cation analysis, ion exchange chromatography is best. (pharmtech.com)
  • An ion-exchange monomer including an anion exchange group and a cation exchange group, 2-methacryloyloxyethyl- phosphoryl choline (MPC) was introduced on the surface of uniformly sized, hydrophilic macro-porous polymer particle. (go.jp)
  • SMT WCX Bulk Packings are silica-based Weak Cation eXchange packing materials developed for separation of cationic compounds. (thomassci.com)
  • It is of two types- cation exchange and anion exchange. (sigmaaldrich.com)
  • Our selection includes a series of more than 38 different multi-anion and multi-cation reference materials solutions. (sigmaaldrich.com)
  • We offer 15 TraceCERT ® cation certified reference material solutions, including ammonium and some of the most commonly tested alkali, alkaline earth, and transition metal ions. (sigmaaldrich.com)
Strongly basic anion exchange resin2
  • DIAION™ HPA25L is a highly porous type strongly basic anion exchange resin. (biokal.com)
  • AmberChrom ™ 1X8 is a strongly basic anion exchange resin which is used as an absorbent in solid phase extraction. (sigmaaldrich.com)
Amperometric Detection1
HPLC7
  • Di-n-butyl phthalate may be determined by high resolution gas chromatography with an electron capture detector (HRGC/ECD) (EPA 1984b), gas chromatography/mass spectrometry (GC/MS) (EPA 1994a), gas chromatography with a flame ionization detector (GC/FID) (NIOSH 1994), gas chromatography/Fourier transform infrared spectrometry (GC/FTIR) (EPA 1994b), high performance liquid chromatography/mass spectrometry (HPLC/MS) (Coldham et al. (cdc.gov)
  • 1998), or high performance liquid chromatography/ion-trap mass spectrometry (HPLC/Ion Trap-MS) (Coldham et al. (cdc.gov)
  • mono-n-butyl phthalate is chromatographically resolved by reverse-phase HPLC, detected by negative ion atmospheric pressure chemical ionization (APCI) tandem mass spectrometry, and quantified by isotope dilution. (cdc.gov)
  • The wide variety of equipment, columns, eluent and operational parameters involved makes high performance liquid chromatography (HPLC) method development seem complex. (pharmtech.com)
  • Based on high purity silica bonded with an ion-exchange ligand, Hypersil GOLD SAX HPLC columns provide outstanding peak shape and sensitivity. (fishersci.com)
  • Based on the change in existing form of poly-MPC modified on the surface of stationary phases, ion-exchange properties of poly-MPC were controllable to result in interesting selectivity changes in HPLC. (go.jp)
  • Phospholipids are extracted and deacylated to glycero-head groups, which are then separated by anion-exchange HPLC and detected by suppressed conductivity measurements. (elsevierpure.com)
Detection4
  • Otherwise, several strategies can be used to increase signal to noise ratio by combining the elution conditions, column chemistries and detection methods to remove interference from other major anions. (thermofisher.com)
  • U.S. EPA Method 300.0, written in 1993, uses ion chromatography with suppressed conductivity detection. (thermofisher.com)
  • On-chip sample injection, separation, and detection of seven common anions are successfully demonstrated with a sensitivity of 1 ppm. (caltech.edu)
  • Sulphate was measured in diets, faeces (ileal effluent in ileostomists), and urine by anion exchange chromatography with conductivity detection. (bmj.com)
Resin8
  • Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). (wikipedia.org)
  • In solution, the resin is coated with positively charged counter-ions (cations). (wikipedia.org)
  • This ion-exchange resin has not been specially processed nor cleaned. (sigmaaldrich.com)
  • CrVI was extracted from standard solutions and simulated samples and retained on an ion exchange resin via ultrasonic extraction in alkaline solution. (cdc.gov)
  • CrVI was separated from trivalent- chromium (CrIII) and other cations on a strong anion exchange resin using a slightly basic ammonium-sulfate buffer solution. (cdc.gov)
  • Breakthrough of CrVI from the anion exchange resin and recovery of CrVI were examined by separating, eluting, and analyzing solutions of various buffer strengths spiked with CrVI and CrIII. (cdc.gov)
  • Over 95% of the CrVI was eluted from the anion exchange resin using 5 milliliters of the buffer solution containing 0.1M ammonium and 0.5M ammonium-sulfate. (cdc.gov)
  • Ion chromatography (IC) separates charged molecules based on their affinity to the ion-exchange resin. (sigmaaldrich.com)
Mass spectrometry2
  • In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr. (chromatographytoday.com)
  • The feasibility of using anoxic anion exchange chromatography (AEC) coupled to dynamic reaction cell-inductively coupled plasma-mass spectrometry to separate aqueous selenide was investigated with the goal of quantifying this anion to determine its importance in reducing waters. (trentu.ca)
Conductivity1
  • An integrated ion liquid chromatography chip is demonstrated, which has on-chip column, filters, injection structure, and conductivity detector. (caltech.edu)
Size-exclusion chro3
Inorganic2
  • The method is the original IC standard for inorganic anion analysis in different types of waters, such as ground, surface, drinking and wastewater. (thermofisher.com)
  • Our portfolio includes a comprehensive range of more than 40 different single component inorganic and organic anion standard solutions from the TraceCERT ® brand. (sigmaaldrich.com)
Separations2
  • Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. (fishersci.com)
  • We describe here simple anion-exchange chromatographic approaches to accomplish these separations. (chromatographytoday.com)
Proteins5
  • Anion exchange chromatography is commonly used to purify proteins, amino acids, sugars/carbohydrates and other acidic substances with a negative charge at higher pH levels. (wikipedia.org)
  • Copper ions bind to biomolecules (e.g., peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. (mdpi.com)
  • Ion exchange (IEX) chromatography is used to separate proteins based on their net charge at a particular pH. (hydrocarbononline.com)
  • Phosphatidylinositol 4,5-biphosphate (PIP 2 ) modulates the function of numerous ion transporters and channels, as well as cell signaling and cytoskeletal proteins. (elsevierpure.com)
  • TcDH contains a unique metal site comprising Crated into proteins for the purposes of copper trafficking three copper ions in a configuration that enables two-electron and storage (3, 4) and electron transport and catalysis (5-7). (docslib.org)
Carbohydrates1
Ligand1
  • Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel. (brighton.ac.uk)
Aqueous3
  • These quaternary amine strong anion-exchange columns are highly stable for aqueous and low pH mobile phases. (fishersci.com)
  • Selenide is cited as a geochemically important selenium (Se) species, but it is unknown whether selenide is a stable aqueous ion in natural waters. (trentu.ca)
  • IC determines the ions in aqueous solutions down to the parts per trillion (ppt) range. (sigmaaldrich.com)
Seven common anions1
  • This method has Part A and B: Part A for the seven common anions and Part B for disinfection byproduct (DBP) anions including chlorate, chlorite, and bromate. (thermofisher.com)
Membrane1
Protein6
  • If the pH is above the pI, the protein will have a negative charge and bind to the matrix in an anion exchange column. (wikipedia.org)
  • Chapter Eight - Using Ion Exchange Chromatography to Purify a Recombinantly Expressed Protein. (wikipedia.org)
  • Ion Exchange PhyTip ® columns is used for efficient parallel purification of small protein samples. (biotage.com)
  • Development of a multi-layering protein grafting process on miniaturized monolithic columns for weak affinity nano liquid chromatography application purposes. (isa-lyon.fr)
  • The high concentration of salt ions (such as SO4 and NH4 of ammonium sulfate) have strong hydration power, which can seize the hydration layer of protein molecules and make The "loss of water", so the protein colloids coagulate and precipitate out. (medicilon.com)
  • This application note discusses a method that uses an ACQUITY UPLC H-Class Bio System and a Waters Protein-Pak Hi Res Q strong anion-exchange column to separate and quantify plasmid isoforms. (selectscience.net)
Disaccharides1
Resins1
  • Anion exchange resins will bind to negatively charged molecules, displacing the counter-ion. (wikipedia.org)
Molecules1
  • One of the most significant current discussions in Cu biochemistry relates to the mechanisms of the positive and negative actions of Cu ions based on the generation of reactive oxygen species, including radicals that can interact with DNA molecules. (mdpi.com)
Inositol1
Elution1
  • In this study, we validated gradient elution in microchip liquid chromatography (LC) and investigated the retention and bandwidth predictions of fluorescently labeled aliphatic amines in fast gradient elution chromatography using semi-empirical retention models. (go.jp)
Liquid3
Column3
  • Bromate can be separated from other anions based on different ion chromatography (IC) column chemistries, then detected by a variety of techniques. (thermofisher.com)
  • In EPA Method 302.0, bromate is partially resolved from matrix ions on the first dimension column (4mm internal diameter or i.d. (thermofisher.com)
  • The column is packed with 7 µm anion-exchange beads with a slurry packing technique. (caltech.edu)
Analysis2
  • Atomic absorption spectral analysis of malabarin raveled the association of zinc metal ion. (uni-mysore.ac.in)
  • It is one of the best methods to offer quantitative analysis of ions at the ppt level. (sigmaaldrich.com)
Strong2
  • Reverse phase is the choice for the majority of samples, but if acidic or basic analytes are present then reverse phase ion suppression (for weak acids or bases) or reverse phase ion pairing (for strong acids or bases) should be used. (pharmtech.com)
  • Three oligosaccharides were purified by strong-anion exchange high-performance chromatography. (maigrir-regime-musculation.com)
Electron1
  • However, some pre- three copper ions located at the corners of a near-isosceles tri- vious microbiological evidence showed that thiocyanate may be angle and facilitates a direct thiocyanate conversion into cya- degraded by cell-free extracts of haloalkaliphilic thiocyanate- nate, elemental sulfur, and two reducing equivalents without utilizing SOB in the presence of an electron acceptor via a involvement of molecular oxygen . (docslib.org)
Copper2
  • oxidation of thiocyanate ion to cyanate and elemental sulfur Malfunctioning of copper homeostasis leads to a number of disease states (8, 9). (docslib.org)
  • Here we present an unpreviously reported type of a Copper enzymes comprise a rather diverse, but not numerous, polynuclear center consisting of three copper ions, which form group of biocatalysts. (docslib.org)
Enzyme1
  • In vitro synthesis of RNA requires a purified DNA plasmid, purified RNA polymerase, metal-ion enzyme cofactors, and raw-material nucleotides. (bioprocessintl.com)
Process2
  • Numerical method is widely used for solving the mechanistic models of chromatography process, but it is time-consuming and hard to response in real-time. (bvsalud.org)
  • In this research, a proof-of-concept study was carried out to apply PINN to chromatography process simulation. (bvsalud.org)
Breakthrough1
  • A PINN for LKM (LKM-PINN) consisting of four neural networks, 12 layers and 606 neurons was then used for the simulation of breakthrough curves of chromatography processes. (bvsalud.org)
Analytes1
Negative ion2
Phase2
Method1
Methods1
  • It should be mentioned that anions such, as bromide, cannot be determined in EPA 317 and 326, which is a major limitation of these methods. (thermofisher.com)
Basic1
Article1