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Chromatography, Ion ExchangeAnion Exchange ResinsChromatography, AffinityChromatography, GelChromatography, High Pressure LiquidAnionsMolecular WeightElectrophoresis, Polyacrylamide GelChromatographyMolecular Sequence DataAmino Acid SequenceKineticsHydrogen-Ion ConcentrationSubstrate SpecificityAmino AcidsCattleCarbohydrate SequenceCarbohydratesOligosaccharidesGelsChromatography, LiquidMass SpectrometryRecombinant ProteinsChromatography, GasCarbohydrate ConformationChromatography, Thin LayerSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationChromatography, DEAE-CelluloseEscherichia coliPeptide FragmentsBase SequenceGlycoproteinsSepharoseAnion Exchange Protein 1, ErythrocyteCloning, MolecularHydrolysisProtein BindingAnion Transport ProteinsIsoenzymesChromatography, AgaroseGlycosylationIsoelectric FocusingCytosolBlotting, WesternIsoelectric PointBinding Sites4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidChloride-Bicarbonate AntiportersRabbitsChloridesSulfatesSLC4A ProteinsCells, CulturedCarrier ProteinsAntiportersIon ExchangeEnzyme ActivationTrypsinMacromolecular SubstancesTemperatureLectinsBicarbonatesChemical FractionationCell LinePeptidesCell MembraneAffinity LabelsSequence Homology, Amino AcidLiverBinding, CompetitiveErythrocytesGas Chromatography-Mass SpectrometryIon Exchange ResinsSpectrophotometry, UltravioletChemistryImmunodiffusionMagnetic Resonance SpectroscopyChemical PhenomenaSolubilityPolysaccharidesProtein ConformationPlant LectinsAmmonium SulfateBacterial ProteinsSpectrometry, Mass, Electrospray IonizationElectrophoresis, Gel, Two-DimensionalSwineEnzyme StabilityProteinsTandem Mass SpectrometryIndicators and ReagentsBiological TransportMembrane ProteinsRecombinant Fusion ProteinsDNAGlycoside HydrolasesMethodsBlood ProteinsErythrocyte Membrane
PurificationResinsHydrophobicRelative binding afCation exchangeFiltration chromatographyHPLCSilica gelProteinAnalyticalChromatographicProteinsLigandAgaroseSeparationExchangersPositively chargedStationary phaseHydrophilicImpuritiesSeparationsWeakColumnsColumnEluteSubstancesSeparateFluidIonicLiquidHighInteractionsTechniqueSizeMethodTechniquesRange
Purification14
  • Purification by affinity chromatography using immobilized sheep IgG, IgM, IgA. (yoproteins.com)
  • Purification by gel filtration and anion-exchange chromatography to remove protein aggregates and free peroxidase. (yoproteins.com)
  • An alternate approach using affinity and anion-exchange column chromatogrpahy (macro-prep DEAE) provided a 125-fold purification. (uleth.ca)
  • The widely used method of extracting lectins from marine samples is a simple two-step process employing a polar salt solution and purification by column chromatography. (bvsalud.org)
  • Explore a complete line of chromatography resins and media with various base bead and ligand chemistries for your lab-scale and bioproduction purification needs. (bio-rad.com)
  • From high-throughput resin screening to small-scale method development and scale-up optimization, find chromatography resins in prepacked formats for the entire purification development cycle. (bio-rad.com)
  • AEX chromatography resins and membrane adsorbers are frequently used for downstream purification in the biotechnology industry. (biopharminternational.com)
  • Protein A chromatography is a very robust purification procedure and is used as a capture step due to its specificity. (tosohbioscience.com)
  • For the downstream workflow of virus production, affinity chromatography purification has been found to effectively remove impurities such as plasmid DNA and host cell RNA/DNA. (biotage.com)
  • The CaptureSelect TM AAVX affinity matrix is specifically designed to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. (biotage.com)
  • PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step. (biotage.com)
  • PhyTip columns process samples by dual flow chromatography which is a gentle purification process for high levels of biologically active protein. (biotage.com)
  • Confirmation of GC analysis is typically accomplished using gas chromatography with mass spectroscopy (GC-MS). The preparation of samples for GC analysis typically involves sample extraction with an organic solvent (e.g., hexane or diethyl ether) and purification of the extract (e.g., by column chromatography or further extraction). (cdc.gov)
  • Hydrophobic Interaction Chromatography (HIC) is a powerful tool for the process purification of biomolecules. (tosohbioscience.com)
Resins15
  • Purify affinity-tagged proteins, monoclonal antibodies, and other biomolecules with a range of affinity resins including immobilized metal affinity chromatography (IMAC), Protein A, and activated resins. (bio-rad.com)
  • Purify charged and polar molecules with anion and cation exchange chromatography (AEX, CEX) resins available in various particle sizes, ionic forms, and binding capacities. (bio-rad.com)
  • Experience unparalleled selectivity, resolution, yield and ease-of-use for a variety of biomolecules with the unique separation properties of our multimodal chromatography resins. (bio-rad.com)
  • Explore a wide selection of size exclusion (or gel filtration) chromatography resins for diverse applications, including those requiring use of organic eluents. (bio-rad.com)
  • The performance of commonly used AEX resins and membrane adsorbers, detailing dynamic binding capacity performance, efficiency, and a new disposable option for packed bed chromatography are compared. (biopharminternational.com)
  • Multiple product formats are commercially available, including chromatography resins, which are packed into chromatography columns, and membrane adsorbers, which are supplied in self-contained plastic housings. (biopharminternational.com)
  • On the other hand, the re-use of resins is well established for traditional packed bed chromatography and this facilitates lower overall material costs. (biopharminternational.com)
  • High performance, rigid resins with a higher volumetric throughput capability provide an advantage over soft-gel resins allowing for properly sized columns with smaller footprints similar to membranes. (biopharminternational.com)
  • Protein A resins are the most frequently used affinity resins in biomanufacturing. (tosohbioscience.com)
  • Tosoh Bioscience offers two Protein A affinity resins, both based on alkaline stable, recombinant ligands coupled to the proven TOYOPEARL polymethacrylate matrix. (tosohbioscience.com)
  • Separation technique in which the stationary phase consists of ion exchange resins. (lookformedical.com)
  • The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. (lookformedical.com)
  • TOYOPEARL and TSKgel Ether hydrophobic interaction chromatography (HIC) resins have the highest hydrophilicity (lowest hydrophobicity) of the HIC ligands offered by Tosoh Bioscience. (tosohbioscience.com)
  • TOYOPEARL and TSKgel Phenyl hydrophobic interaction chromatography resins are located in the middle of the hydrophobicity range of the five HIC ligands offered by Tosoh Bioscience. (tosohbioscience.com)
  • TOYOPEARL Butyl hydrophobic interaction chromatography (HIC) resins have the second highest hydrophobicity of the HIC ligands offered by Tosoh Bioscience. (tosohbioscience.com)
Hydrophobic4
  • So in water hydrophobic non polar substances appear toe have a strong net attraction or strong net affinity for each other. (pearson.com)
  • So we already said in our previous video that the hydrophobic effect is not explained by the strong net affinity of hydrophobic substances for each other. (pearson.com)
  • Here, we use SDC approach with CIM® C4 HLD monoliths under hydrophobic interaction chromatography (HIC) conditions to separate plasmid DNA (pDNA) isoforms under overloading conditions, where supercoiled (SC) isoform acts as a displacer of open circular (OC) or linear isoform. (biaseparations.com)
  • TOYOPEARL PPG (polypropylene glycol) hydrophobic interaction chromatography (HIC) resin has the second highest hydrophilicity (second lowest hydrophobicity) of the HIC ligands offered by Tosoh Bioscience. (tosohbioscience.com)
Relative binding af1
  • Sample displacement chromatography (SDC) is a chromatographic technique that utilizes differences in relative binding affinities of components in a sample mixture under chromatographic conditions. (biaseparations.com)
Cation exchange3
  • so a negatively-charged cation exchange resin is chosen. (blueprintprep.com)
  • For example, if a cation exchange resin is chosen, all proteins that are positively charged will bind to the negatively charged column beads. (blueprintprep.com)
  • SMT WCX Bulk Packings are silica-based Weak Cation eXchange packing materials developed for separation of cationic compounds. (thomassci.com)
Filtration chromatography3
  • The purest enzyme preparation (354.4-fold) was obtained through a combination of affinity (Affi-gel blue) and gel filtration chromatography. (uleth.ca)
  • In this study, AKR from C. auris (CaAldO) was successfully expressed and purified using Ni-NTA affinity, Q anion exchange, and gel-filtration chromatography. (bdjn.org)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
HPLC2
Silica gel4
  • YMC-Pack Diol is a size exclusion chromatography column utilizing a silica gel base. (thomassci.com)
  • LiChrosorb® RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. (thomassci.com)
  • The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
  • The extracted DCA and MCA was then derivatized by ethylation or methylation and purified by column chromatography on silica gel using an ethyl acetate/benzene eluent. (cdc.gov)
Protein8
  • FBP was purified from osmotic-shock fluid using anion-exchange fast protein liquid chromatography (FPLC). (microbiologyresearch.org)
  • The monomeric protein (M r 34200 by SDS-PAGE and 37700 by gel-filtration FPLC) bound D-[U -14 C]-fructose stoichiometrically (1.17 nmol nmol FBP -1 ) and with high affinity (K D 0.49 μM) as shown by equilibrium dialysis. (microbiologyresearch.org)
  • TSKgel Protein A-5PW affinity columns for direct analysis of mAb titer. (selectscience.net)
  • so a positively charged anion exchange resin is chosen to capture this protein. (blueprintprep.com)
  • Protein A Chromatography relies on the specific and reversible binding of antibodies to an immobilized protein A ligand. (tosohbioscience.com)
  • Our AAV PhyTip columns are packed with a high capacity AAV affinity resin, which routinely generates purified samples of his-tagged protein with 60 - 80% sample recovery. (biotage.com)
  • Our Protein A PhyTip columns comes with dual flow chromatography protocols that have be validated with real protein and can be used on any automated liquid handling system. (biotage.com)
  • HIC is an excellent complement to ion exchange and size exclusion chromatography, particularly when protein isoforms exist or when feedstock impurities are of similar isoelectric point or molecular weight. (tosohbioscience.com)
Analytical1
  • Electrophoretic conditions and, more importantly, matrix used for sample dilution before gel loading can affect analytical results. (biaseparations.com)
Chromatographic1
  • We describe here simple anion-exchange chromatographic approaches to accomplish these separations. (chromatographytoday.com)
Proteins5
  • Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
  • To be able to gather a purified proteins remedy of CST4 extremely, both Ni-nitrilotriacetic acidity affinity chromatography and anion exchange chromatography had been used using 500 mL of cell tradition filtrate (previously gathered and filtered). (informationalwebs.com)
  • When an ion exchange chromatography column is loaded with a sample at a particular pH, all proteins that are appropriately charged will bind to the resin. (blueprintprep.com)
  • Even very similar components, such as proteins that may only vary by a single amino acid, can be separated with chromatography. (blueprintprep.com)
  • The selectivity differences exploited by HIC can also be used after affinity separations in which closely related proteins with similar recognition sites are not distinguishable by the affinity ligand. (tosohbioscience.com)
Ligand1
  • The dextran-coated magnetic nanoparticles are further coupled to either polymyxin B or histidine to provide a positively charged ligand which enhances the affinity to the negatively charged endotoxin. (springeropen.com)
Agarose2
  • After confirmation of the dual digestive function, agarose gel electrophoresis, and gene sequencing, 1 gL?1 CST4-pcDNA3.1 was transformed into COS-7 cells by lipofectamine 2000, cultivated in DMEM containing 10% FBS at 37C with 5% CO2 for 72 h. (informationalwebs.com)
  • Agarose gel electrophoresis (AGE) analysis is an important method for monitoring of plasmid DNA (pDNA) quality, with ability to separate pDNA isoforms (sc, oc, lin, multimer). (biaseparations.com)
Separation5
  • There are several ways to model the separation mechanism: partition, adsorption, ion exchange, and size exclusion. (springer.com)
  • NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
  • For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
  • Figure 2 shows how HILIC complements other areas of chromatography and extends the range of separation options. (springer.com)
  • Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)
Exchangers1
  • Two anion exchangers, CIMmultus PrimaS and H-Bond, were tested for their capacity for endotoxin removal in comparison to well known strong anion exchanger, CIMmultus QA. (biaseparations.com)
Positively charged2
Stationary phase3
Hydrophilic1
  • Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
Impurities2
Separations1
Weak1
  • The TSKgel DNA-NPR weak anion exchange column is designed to analyze nucleic acids. (selectscience.net)
Columns5
  • In most cases, traditional soft-gel FT columns are sized for the optimization of volumetric throughput to improve operating flow rate and decrease process bottlenecks, rather than sized for actual impurity binding capacity. (biopharminternational.com)
  • In addition, chromatography columns are easily scalable. (biopharminternational.com)
  • TSKgel FcR-IIIA-NPR affinity columns for direct analysis of mAb activity. (selectscience.net)
  • Chromatography on thin layers of adsorbents rather than in columns. (lookformedical.com)
  • The CaptureSelect TM AAVX PhyTip® columns with our proprietary Dual Flow Chromatography are compatible with most 8, 12, and 96-channel automation liquid handling robots. (biotage.com)
Column1
  • CIMmultus® EV, an AEX chromatography monolith column, can separate EVs in subpopulations based on charge and offers insight into the heterogenicity of particles. (biaseparations.com)
Elute2
Substances2
  • HILIC also allows the analysis of charged substances, as in ion chromatography (IC). (springer.com)
  • Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. (lookformedical.com)
Separate2
Fluid2
Ionic1
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
Liquid3
High4
  • A high-performance anion exchange resin performs well compared with membranes. (biopharminternational.com)
  • Late-phase process redesign or a complete switch to packed bed chromatography may be required due to high consumable costs or limited product formats not allowing for linear scale-up. (biopharminternational.com)
  • The Open University provides Anthias Consulting with the Training Facility for the high-quality, affordable, instrument-independent training courses that can boost your knowledge, confidence and results in all aspects of gas chromatography and GC-MS. These courses have a strong practical focus which can be applied the instant you return to your lab and are applicable to all levels of experience. (anthias.co.uk)
  • Three oligosaccharides were purified by strong-anion exchange high-performance chromatography. (maigrir-regime-musculation.com)
Interactions1
  • An important part of affinity chromatography design is choosing the proper binding material, so be on the lookout for any clues the tests gives us as to the interactions used by the target molecules. (blueprintprep.com)
Technique1
Size1
  • A mixture (including the target molecules) is filtered through a gel, inside of which are spherical beads containing pores of a specific size. (blueprintprep.com)
Method1
  • Different methods of the LAL assay are available: the gel-clot assay, the chromogenic substrate method, and the turbidimetric method [ 10 ]. (springeropen.com)
Techniques1
  • While the MCAT will not expect us to be chromatography experts, the test makers expect us to know the fundamentals, how/when to apply appropriate techniques, and will expect us to make conclusions based on reported results. (blueprintprep.com)
Range1
  • The facility boasts a wide range of equipment from multiple manufacturers covering instrumentation in gas chromatography such as comprehensive multi-dimensional gas chromatography, PTV injectors, autosamplers, thermal desorbers and pyrolysers. (anthias.co.uk)