• Apoptosis induced by antineoplastic drug treatment was significantly decreased in SW480/ANGPTL2 compared to control cells.Expression of anti-apoptotic BCL-2 family genes was upregulated in SW480/ANGPTL2 compared to SW480/Ctrl cells.To assess signaling downstream of ANGPTL2 underlying this effect, we carried out RNA sequencing analysis of SW480/ANGPTL2 and SW480/Ctrl cells. (nih.gov)
  • a) Relative mRNA expression of the anti-apoptotic BCL-2 family members BCL-2 (left) and BCL-XL (middle) and of pro-apoptotic BAX (right) in SW480/ANGPTL2-1, SW480/ANGPTL2-2, or SW480/Ctrl cells. (nih.gov)
  • 19) Therefore, we examined the expression of BCL-2 family mRNAs in SW480/ANGPTL2-1 and -2 cells or SW480/Ctrl cells. (nih.gov)
  • 19,20) Therefore, we analyzed that ratio in SW480/ANGPTL2-1, SW480/ANGPTL2-2, and SW480/Ctrl cells and found that BCL-2/BAX and BCL-XL/BAX ratios were greater in both ANGPTL2-overexpressing lines than they were in controls (Fig. 2b). (nih.gov)
  • Recently, several reports suggested that a high BCL-2/BAX or BCL-XL/BAX ratio is positively correlated with progression of several diseases or malignant tumors and that the BCL-2/BAX ratio may serve as a prognostic marker for patients with rectal carcinomas. (nih.gov)
  • The BCL-2 family is a key regulator of programmed cell death. (nih.gov)
  • Kanda, " Angiopoietin 2 stimulates migration and tube-like structure formation of murine brain capillary endothelial cells through c-Fes and c-Fyn," Journal of Cell Science, vol. (thefreedictionary.com)
  • Western Blot, RT-PCR and immunocytochemistry identified MMEC as the major source of Ang-2, at variance with MM cells and CD14+ BM monocytes. (ovid.com)
  • Human and mouse angiopoietin 2 share 85% sequence identity and are 60% identical to their Ang1 homologs. (genetex.com)
  • Circulating angiopoietin 2 correlates with mortality in a surgical population with acute lung injury/adult respiratory distress syndrome," Shock, vol. (thefreedictionary.com)
  • In Multiple Myeloma (MM), serum Ang-2 correlates with disease progression and response to therapy. (ovid.com)
  • Methods and Results- Human umbilical vein ECs (HUVECs), human microvascular ECs (HMECs), or bovine aortic ECs (BAECs) were subjected to either LS (15 dyn/cm 2 ) or OS (±5 dyn/cm 2 ) for 24 hours and used in Matrigel tubule formation or scratch migration assays. (ahajournals.org)
  • Human umbilical vein endothelial cells (HUVECs), bovine aortic ECs (BAECs), and human microvascular EC line (HMEC-1) were grown to confluence and exposed to unidirectional LS (5 or 15 dyn/cm 2 ), OS (±5 or ±15 dyn/cm 2 at 1 Hz frequency), or static control (ST) for 24 hours using a cone-and-plate device as described by us. (ahajournals.org)
  • Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively. (bosterbio.com)
  • In adult mice and humans, angiopoietin 2 is expressed at sites of vascular remodeling. (genetex.com)
  • MM Bone Marrow (BM) sera with high Ang-2 concentration specifically contributed to endothelial cell (EC) activation, while Ang-1 containing sera maintained EC stabilization. (ovid.com)
  • p = .01) in those with an elevated pulmonary dead-space fraction (p = .03 for interaction between pulmonary dead-space fraction and concentration of angiopoietin-2 relative to angiopoietin-1). (biomedsearch.com)
  • CONCLUSIONS: The ratio of concentration of angiopoietin-2 relative to angiopoietin-1 may be a prognostic biomarker of endothelial activation in acute lung injury patients and, along with pulmonary dead-space fraction, may be useful for risk stratification of acute lung injury patients, particularly in identifying subgroups for future research and therapeutic trials. (biomedsearch.com)
  • In further study the more specific attention should be focused on the correlation between cystatin C and even angiopoietin 2 , which appeared to be a relevant predictor of renal dysfunction in acute pancreatitis patients . (thefreedictionary.com)
  • In addition, Ang-2 exerted a weak stimulatory effect on leukocyte migration in the mouse paw. (aspetjournals.org)
  • However, when cell migration was elicited using zymosan, Ang-2 significantly inhibited leukocyte migration. (aspetjournals.org)
  • We have found that Ang-2 administered alone promotes vascular leakage that is characterized by restricted migration of leukocytes, whereas it acts as an inhibitor of zymosan-induced cell migration. (aspetjournals.org)
  • BOULEVARD (NCT02699450) 2 is a Phase II study designed to assess the efficacy and safety of RG7716 compared with ranibizumab in people with diabetic macular edema (DME). (chemdiv.com)
  • The density of yellow coloration is directly proportional to the mouse Angiopoietin-2 amount of sample captured in plate. (abcam.com)
  • Get better reproducibility in only 90 minutes with Mouse Angiopoietin 2 ELISA Kit ( ab209883 ) from our SimpleStep ELISA ® range. (abcam.com)
  • NO and prostaglandin E 2 levels in mouse paw following the injection of Ang-2 remained unaltered, suggesting that the action of Ang-2 does not involve these mediators. (aspetjournals.org)
  • Similarly, Ang-2 injected into the mouse air pouch produced only a modest effect on cell extravasation that peaked at 30 min. (aspetjournals.org)
  • While Ang-2 is widely expressed in the mouse embryo, it is restricted postnatally to pro-angiogenic regions including the placenta, ovaries, and uterus. (cloud-clone.com)