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Chromatography, AffinityChromatography, High Pressure LiquidChromatographyChromatography, GelChromatography, Ion ExchangeMolecular WeightElectrophoresis, Polyacrylamide GelChromatography, GasMolecular Sequence DataKineticsAmino Acid SequenceGelsChromatography, Thin LayerChromatography, LiquidChromatography, DEAE-CelluloseChromatography, AgaroseAmino AcidsHydrogen-Ion ConcentrationSubstrate SpecificityMass SpectrometryChemistryChemical PhenomenaGas Chromatography-Mass SpectrometrySepharoseChromatography, PaperIsoelectric FocusingChemical FractionationCattleSpectrophotometry, UltravioletCarbohydratesHydroxyapatitesChromatography, Reverse-PhaseIsoelectric PointCarbohydrate SequenceEscherichia coliTemperatureMagnetic Resonance SpectroscopyImmunodiffusionRabbitsMethodsChemical PrecipitationFermentationLiverHydrolysisAmmonium SulfateOligosaccharidesDrug StabilityElectrophoresis, Gel, Two-DimensionalBase SequenceTrypsinStreptomycesPhysicochemical PhenomenaPeptide FragmentsChemistry, PhysicalSolubilityPlant ExtractsRecombinant ProteinsProtein BindingCentrifugation, Density GradientCloning, MolecularMacromolecular SubstancesSpectrometry, Mass, Electrospray IonizationElectrophoresisGlycoside HydrolasesHot TemperatureIndicators and ReagentsTandem Mass SpectrometryEnzyme StabilityIsoenzymesMolecular StructureSpectrometry, Mass, Fast Atom BombardmentDurapatiteElectrophoresis, DiscGlycoproteinsCarbohydrate ConformationPeptidesPolysaccharidesBinding SitesCountercurrent DistributionSwineImmunoelectrophoresisSpectrophotometryUltracentrifugationSpectrophotometry, InfraredBacterial ProteinsRadioimmunoassayBiological AssayElectrophoresis, Agar GelSpecies SpecificityStereoisomerismCell LineSequence Homology, Amino AcidBrain ChemistryGlycosidesFatty AcidsGlycopeptidesGlycolipidsMicrochemistryCytosolPronase
Thin-layer chromatoPermeationPreparativeExpanded bed adsorpStationary phaseSeparationHPLC ColumnsResinsPurificationAffinityFiltrationLiquidNormal phase chromatoSolventExchange chromatographyProteinsAdsorbentsCompoundsWide range of molecular weightsAnion-exchangeAcetoneSilica columnResinFlorisilCharacterizationEluteGravityPoreInertDetectorSpectroscopyUHPLCSuitableChemistryMixturesTechniquesMethodWidelyMethodsDetectionMixtureParticle sizeHighInorganicSizeMassPorous polymerMolecularElectrophoresis
Thin-layer chromato4
Permeation40
Preparative5
  • In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. (spandidos-publications.com)
  • Preparative high performance liquid chromatography (prep-HPLC) can shorten the production cycle, has relatively low operating costs, and is simple to carry out. (spandidos-publications.com)
  • Amerigo Scientific offers a wide range of high quality products for analytical HPLC, UHPLC, preparative HPLC, as well as nano flow and capillary LC systems. (amerigoscientific.com)
  • Our HPLC accessories used for analytical HPLC, preparative HPLC and UHPLC systems, include HPLC connecting accessories, column accessories, and maintenance tools, which are manufactured through a rigorous process to ensure operational efficiency, system performance and laboratory safety. (amerigoscientific.com)
  • Usage is both analytical for small amounts and preparative for bulk amounts. (lookformedical.com)
Expanded bed adsorp1
Stationary phase20
  • The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. (wikipedia.org)
  • For the dry method, the column is first filled with dry stationary phase powder, followed by the addition of mobile phase, which is flushed through the column until it is completely wet, and from this point is never allowed to run dry. (wikipedia.org)
  • For the wet method, a slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. (wikipedia.org)
  • The individual components are retained by the stationary phase differently and separate from each other while they are running at different speeds through the column with the eluent. (wikipedia.org)
  • The stationary phase or adsorbent in column chromatography is a solid. (wikipedia.org)
  • The most common stationary phase for column chromatography is silica gel, the next most common being alumina. (wikipedia.org)
  • There is an important ratio between the stationary phase weight and the dry weight of the analyte mixture that can be applied onto the column. (wikipedia.org)
  • CHIRALPAK IE-3 / SFC, CHIRALPAK IE / SFC uses a stationary phase in which an amylose derivative (Amylose tris (3,5-dichlorophenylcarbamate)) is immobilized on a silica gel carrier. (thelabstore.co.uk)
  • Chromatography adsorbents are used in the stationary phase of the process. (sorbeadindia.com)
  • Getting the best possible results in the high-performance liquid chromatography (HPLC), UHPLC or LC-MS analysis of small molecules depends on the selection of the best suitable stationary phase and mobile phase conditions. (sigmaaldrich.com)
  • The HPLC column is packed with either fully porous silica particles, superficially porous silica particles, polymeric particles or consists of a monolithic silica rod as the stationary phase. (sigmaaldrich.com)
  • Chromatography is essentially a separation technique - the sample is carried along by a mobile phase (either gas or liquid) and the components of the sample mixture are separated due to their affinity for the stationary phase. (chromatographytoday.com)
  • In size exclusion chromatography, the stationary phase is. (web.app)
  • The selectivity and separation efficiency are decided by the composition of the mobile and stationary phase and the column sizes. (amerigoscientific.com)
  • Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. (lookformedical.com)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
  • A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. (lookformedical.com)
  • It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
  • Typically, samples are injected into a mobile phase and is carried through an analytical column where components are separated based upon their relative affinities for a liquid mobile phase and the solid stationary phase of the column. (cbrnetechindex.com)
  • The guard column is usually a short column packed with a similar stationary phase. (galaklc.com)
Separation18
  • Silica gel may be used in separation of single-walled carbon nanotube (SWNT) by simple filtration. (sigmaaldrich.com)
  • A faster flow rate of the eluent minimizes the time required to run a column and thereby minimizes diffusion, resulting in a better separation. (wikipedia.org)
  • These diol groups provide the basis for separation of glycated and non-glycated components by boronate affinity chromatography 11a,11b,11c. (cdc.gov)
  • Immobilised metal affinity chromatography (IMAC) has been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. (biokal.com)
  • However, the quality of those measurements is still dependent on the quality of the separation, which itself remains dependent on the column. (pharmaceutical-technology.com)
  • Good resolution depends on matching the column pore size to the sample of interest and an ideal separation requires no interaction between the sample and the column material. (pharmaceutical-technology.com)
  • Viewers will learn about GPC/SEC and understand how the best measurements require powerful detectors and a good separation through the columns. (pharmaceutical-technology.com)
  • Hamilton offers 21 polymer-based stationary phases as well as two silica gels (C8 and C18) to satisfy most separation/purification needs. (hamiltoncompany.com)
  • Supel™ Carbon LC U/HPLC columns packed with porous graphitic carbon (PGC) particles, used for the HPLC, UHPLC, UPLC, and LC-MS separation of polar or charged compounds at high temperatures (up to 250 ˚), pressures up to 700 bar, and the mobile phase pH range of 1-14. (sigmaaldrich.com)
  • Separation of Salicylic acid, meets analytical specification of Ph. (sigmaaldrich.com)
  • For separation of polar compounds hydrophilic interaction chromatography (HILIC) and normal-phase chromatography are suitable as well, with HILIC being the preferred method. (sigmaaldrich.com)
  • For the separation of ionic compounds, ion-exchange separation modes and for inorganic anions or cations, ion chromatography can also be used. (sigmaaldrich.com)
  • Gpc columns perform a separation based on the molecular size of polymer. (web.app)
  • Theory of separation a column is made up of swollen gel particles and the solvent used to swell the gel in a suitable tubular container. (web.app)
  • We offer monodisperse chromatography media for usage from analytical to process scale separation. (amerigoscientific.com)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
  • The results show that comprehensive two-dimensional liquid chromatography system is of great importance and high value in the separation of complex TCM. (edu.hk)
  • GC/MS analysis allows separation of compounds based on their retention time within a chromatography column and identification of each compound by the characteristic fragments into which a given chemical is broken following ionization of the compound. (medscape.com)
HPLC Columns8
  • Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil GOLD™ SAX HPLC columns. (fishersci.com)
  • Based on high purity silica bonded with an ion-exchange ligand, Hypersil GOLD SAX HPLC columns provide outstanding peak shape and sensitivity. (fishersci.com)
  • As part of Shodex, Leah provides sales and technical services for high-performance liquid chromatography (HPLC) columns produced by the Showa Denko Group. (pharmaceutical-technology.com)
  • High performance liquid chromatography (HPLC) columns use higher pressures and smaller solid particles, which not only overcome some limitations of standard LC, but also allow for more effective separations and wider applications, such as pharmaceutical factory, environment, forensics, and chemical industry. (amerigoscientific.com)
  • HPLC columns are the core components of liquid chromatographic analysis. (galaklc.com)
  • The following seven issues should be focused on during the usage of HPLC columns. (galaklc.com)
  • Some HPLC columns cannot be backflushed. (galaklc.com)
  • HPLC columns should be cleaned with a suitable solvent after use. (galaklc.com)
Resins4
  • The MCI Gel™ size exclusion columns and resins are based on hydrophilic polyhydroxymethacrylates suitable for the analysis and purification of water soluble polymers like oligosacharides, proteins, PEG etc. (biokal.com)
  • On this page: List of available columns and resins, followed by some sample chromatograms. (biokal.com)
  • Our specialty resins are offered in a wide variety of hardware dimensions, and while these sizes cover many applications in varying science focuses, you may need a specific Hamilton resin in a column size we don't offer as off-the-shelf. (hamiltoncompany.com)
  • Our packings and media mainly consist of silica gels or polymeric resins for normal phase, reversed phase, affinity, ion exchange, and hydrophilic interaction chromatography. (amerigoscientific.com)
Purification4
  • The Optio IMAC SepFast HighRes column (16 x 100 mm) has a particle size of 20 - 50 µm and is designed and fabricated for high performance chromatographic purification of proteins in packed columns, particularly in FPLC systems. (biokal.com)
  • various drawbacks have been identified in the purification techniques of ion resin adsorption ( 12 , 13 ), silica gel column chromatography ( 14 ) and supercritical extraction ( 15 ). (spandidos-publications.com)
  • Liquid chromatography (LC) is a common purification technique with liquid mobile phases. (amerigoscientific.com)
  • This can be explained by the difficulty involved in isolating sufficient purified quantities of these compounds because of their relatively small concentrations in cannabis plants and extracts-hence the need for accurate and high-throughput purification methods able to isolate sufficient quantities of pure cannabinoids for the production of standards for use in medicinal, analytical, and regulatory applications (8,13). (chromatographyonline.com)
Affinity1
  • The Primus instrument is a fully automated glycohemoglobin analyzer, which utilizes the principle of boronate affinity high performance liquid chromatography (HPLC)P 11dP. (cdc.gov)
Filtration3
  • as molecular mass standard in analytical gel filtration chromatography for Superdex ® 75 10/300 GL gel filtration column. (sigmaaldrich.com)
  • It can be used as a gel filtration molecular weight marker. (sigmaaldrich.com)
  • Filtration must be performed before using the instrument to prevent dust and other particulates from running the columns and interfering with the detectors. (eag.com)
Liquid12
Normal phase chromato1
Solvent10
  • Column chromatography can be done using gravity to move the solvent, or using compressed gas to push the solvent through the column. (wikipedia.org)
  • A solvent reservoir may be attached at the top of the column. (wikipedia.org)
  • The mobile phase or eluent is a solvent or a mixture of solvents used to move the compounds through the column. (wikipedia.org)
  • IE solvent-resistant chiral column, the amylose-tris (3,5-dichlorophenylcarbamate) covalently bonded to the surface of the silica gel. (thelabstore.co.uk)
  • Unrestricted use of solvents, a wide range of dissolution conditions, the use of any miscible solvent as a mobile phase, dissolved samples, chiral column regeneration. (thelabstore.co.uk)
  • This reflects the component nature of the instrumentation which often includes solvent degassers, autosamplers, and thermostatted column compartments in addition to the required solvent pumps, sample injector ports, and detectors. (cbrnetechindex.com)
  • Analytical methods used for this purpose often involve tedious and solvent-intensive sample preparation prior to analytical determination, making them unattractive. (bvsalud.org)
  • A rapid and solvent-sparing offline solid phase extraction (SPE) gas chromatography (GC) flame ionization detection (FID) method for the determination of endogenous n-alkanes in vegetable oils was, therefore, optimized and validated. (bvsalud.org)
  • And when using an eluent with a high sub-elution capacity to flush the column, the replacement of the mobile phase in the flow path system should be gradually transitioned with a solvent that is miscible. (galaklc.com)
  • When storing the HPLC column, the column should be filled with acetonitrile or methanol, and the column joints should be tightened to prevent the solvent from evaporating and drying. (galaklc.com)
Exchange chromatography2
Proteins2
  • Analytical and micropreparative high-performance gel chromatography of proteins with a short column. (nist.gov)
  • When a solution of proteins (e.g. hemolysate) is passed through the column, the glycated component is retained by the complexing of its diol groups with the boronate. (cdc.gov)
Adsorbents7
Compounds14
  • compounds move through the column at different rates, allowing them to be separated into fractions. (wikipedia.org)
  • A thin-layer chromatograph can show how a mixture of compounds will behave when purified by column chromatography. (wikipedia.org)
  • The composition of the eluent flow can be monitored and each fraction is analyzed for dissolved compounds, e.g. by analytical chromatography, UV absorption spectra, or fluorescence. (wikipedia.org)
  • Contrary to common misconception, methanol alone can be used as an eluent for highly polar compounds, and does not dissolve silica gel. (wikipedia.org)
  • Following this, he continued for another year using chromatography to study the uptake of these same compounds into the blood. (pharmaceutical-technology.com)
  • Gas chromatography-mass spectrometry (GC-MS) analyses has resulted in identification of 86 compounds, which were further confirmed through the Retention Indices. (org.pk)
  • This characteristic makes it a great compound for column chromatography as it can bind with several different chemical compounds effectively. (sorbeadindia.com)
  • Silica gel is an effective adsorbent that binds to different compounds and helps isolate them. (sorbeadindia.com)
  • Different instruments can be used to purify compounds on a preparatory scale, separate compounds on an analytical scale, or study the ions present in a mixture. (monmouthcollege.edu)
  • LC has wider applicability than gas chromatography in analyzing compounds with thermal instability or high boiling points. (amerigoscientific.com)
  • We provide different nano flow LC columns suitable to couple with mass spectrometry (MS) systems, allowing to detect complex biological samples, from small to high molecular weight compounds, which can be used in proteome, lipidome and metabolome research. (amerigoscientific.com)
  • This is particularly important for the production of (new) analytical-grade standards, used as reference materials, and for the execution of clinical trials to test the biological activity of the single compounds that could potentially be used as new drugs or active pharmaceutical ingredients (APIs). (chromatographyonline.com)
  • A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
  • An analytical technique for resolution of a chemical mixture into its component compounds. (lookformedical.com)
Wide range of molecular weights2
  • Using this form of size exclusion chromatography, a wide range of molecular weights can be characterized when compared to retention times of standard molecular weights. (eag.com)
  • Shimpack gpc 80m mixed gel columns 80mc, 80md mixed gel columns that produce linear calibration curves over a wide range of molecular weights. (web.app)
Anion-exchange1
  • These quaternary amine strong anion-exchange columns are highly stable for aqueous and low pH mobile phases. (fishersci.com)
Acetone1
  • Inside the analytical scale biotransformations using selected strains, 0.two g of 1 dissolved in two ml of acetone was equally distributed amongst flasks with fungal cultures. (calpaininhibitor.com)
Silica column2
  • For silica column chromatography, this ratio lies within 20:1 to 100:1, depending on how close to each other the analyte components are being eluted. (wikipedia.org)
  • From the project, the team was able to learn essential column chromatography techniques, such as basicification of silica column using ammonia hydroxide, as well as experimental design and trouble shooting methods. (rochester.edu)
Resin1
  • The columns are 7.5 mm x 600 mm (ID x L) packed with 10 µm particle size resin. (biokal.com)
Florisil1
Characterization2
  • After a novel composition is synthesized, the next steps are evaluation of its performance and analytical characterization. (aqueroco.com)
  • Consequently, the lack of a universally accepted standardized analytical method for the chemo- and enantio-selective analysis of phytocannabinoids and the limited number of cannabinoid reference standard materials make it difficult to obtain a comprehensive characterization of the sample in terms of purity, identification, and concentration of the single species. (chromatographyonline.com)
Elute2
  • At the end of the column they elute one at a time. (wikipedia.org)
  • Small molecules typically interact and enter the pores without difficulty and therefore take the longest to elute from the column. (eag.com)
Gravity2
Pore3
  • Its small spherical shaped particle and carefully controlled pore structure allows superior chromatographic resolution in a packed column operation. (biokal.com)
  • Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology. (sigmaaldrich.com)
  • Composed of semipermeable, porous polymer gel beads with well defined range of pore sizes. (web.app)
Inert1
  • Leco N, O AMG Analytical Services utilizes the inert gas fusion method for Nitrogen analysis. (amg-chrome.com)
Detector3
  • The glycated component binds to the boronate, while the non-glycated component passes through the column to the spectrophotometric detector, where it is detected at wavelength of 413-±2 nm. (cdc.gov)
  • The technique involved collection on a solid sorbent or in a midget impinger containing water, desorption with organic solvents and water, derivatization with 1,3-cyclohexadiene or morpholine, and analysis with gas chromatography using a flame ionization detector. (cdc.gov)
  • Presence (peak position) and concentrations (peak magnitude) of ionic solutes can be measured via conductivity detector and ion-exchange columns. (aqueroco.com)
Spectroscopy2
  • In addition, the team also learned analytical techniques such as UV-Vis spectroscopy and analysis. (rochester.edu)
  • Along with spectroscopy, chromatography is one of the major analytical techniques at the disposal of any chemical or biochemical researcher. (chromatographytoday.com)
UHPLC2
  • According to different applications and samples, qualified HPLC and UHPLC columns are available in various materials, particle sizes, and inner dimensions. (amerigoscientific.com)
  • We offer different specifications of HPLC/UHPLC columns and cartridges featuring high analytical resolution and sensitivity. (amerigoscientific.com)
Suitable1
Chemistry1
Mixtures1
  • Moreover, despite the large number of discovered cannabinoids, only roughly 20% are available as analytical standards, with some of them available only as racemic or scalemic mixtures, and not as single enantiomers (8). (chromatographyonline.com)
Techniques4
  • The analytical techniques used were silica gel column chromatography and high-performance size exclusion chromatography. (uniba.it)
  • Analytical Services is an ISO 17025 (UKAS) accredited laboratory who perform both UKAS and NON-UKAS work using XRF/XRD, ICP-OES, AAS, Particle Size Distribution techniques. (amg-chrome.com)
  • Additionally other NON-UKAS analytical techniques are performed such as Microscopy, Chromatography, Classical Gravimetric methods, among others. (amg-chrome.com)
  • AMG Analytical Services offers a number of techniques for particle size measurement from traditional classical methods, through sieve testing to the most popular - laser diffraction. (amg-chrome.com)
Method8
  • Two methods are generally used to prepare a column: the dry method and the wet method. (wikipedia.org)
  • Test results using the NIOSH analytical method S157 for acrylamide (79061) are reported. (cdc.gov)
  • Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. (sigmaaldrich.com)
  • The primary method for detecting methyl parathion and metabolites in biological tissues is gas chromatography (GC) coupled with electron capture (ECD), flame photometric (FPD), or flame ionization detection (FID). (cdc.gov)
  • We'll work with you to ship your used HPLC column back to us using a traceable shipping method. (hamiltoncompany.com)
  • As with any analytical method, sample preparation is crucial and AMG-Analytical was one of the early pioneers of the Borate glass bead technique. (amg-chrome.com)
  • An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. (lookformedical.com)
  • Depending on the composition of the mobile phase and column packing material a variety of samples can be analyzed using this method. (cbrnetechindex.com)
Widely2
  • They are widely used in foodstuffs as gelling agents and a source of fibre. (chromatographytoday.com)
  • LC is a very mature analytical technique and is used widely in industry and environmental applications. (cbrnetechindex.com)
Methods6
  • The purpose of this chapter is to describe the analytical methods that are available for detecting, measuring, and/or monitoring methyl parathion, its metabolites, and other biomarkers of exposure and effect to methyl parathion. (cdc.gov)
  • The intent is not to provide an exhaustive list of analytical methods. (cdc.gov)
  • Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits and/or to improve accuracy and precision. (cdc.gov)
  • Table 7-1 summarizes the analytical methods used to detect methyl parathion and its metabolites in biological tissues and fluids. (cdc.gov)
  • The absence of well-characterized reference standards imposes limitations on the identification and quantification capability of analytical methods. (chromatographyonline.com)
  • AMG Analytical Services carries out several classical wet methods of analysis. (amg-chrome.com)
Detection1
  • These standards are characterized using a battery of analytical tests, including gpc, gpc with light scatter ing detection. (web.app)
Mixture3
Particle size1
  • Silica gel allows a controlled fragmentation leading to form uniform polymer particles with narrow particle size distribution and high bulk density. (sigmaaldrich.com)
High5
  • The low- and high-pressure pumps transfer reagents through the analytical column, with reagent selection executed by a switching valve. (cdc.gov)
  • For the SFC columns CHIRALPAK IE - 3 / SFC, CHIRALPAK IE / SFC have the strength required for SFC specified in the High Pressure Gas Safety Act, and we will attach documents to prove this to the product. (thelabstore.co.uk)
  • Malvern's webinar will discuss how high-quality columns and detectors both contribute to high quality GPC/SEC measurements. (pharmaceutical-technology.com)
  • The AZURA Compact HPLC HPG is a complete analytical high pressure gradient HPLC system. (cbrnetechindex.com)
  • Sudden changes in temperature or dropping the column from a high point can affect the condition of the packing materials inside the column. (galaklc.com)
Inorganic1
  • AMG Analytical Services delivers first class inorganic analytical testing services to a diverse customer base for whom quality and integrity of service is paramount. (amg-chrome.com)
Size3
Mass1
  • If the screening test is positive, then that positive result must be confirmed, typically with gas chromatography/mass spectrometry (GC/MS). (medscape.com)
Porous polymer1
  • The analytical column contains aminophenylboronic acid bonded to a porous polymer support (gel). (cdc.gov)
Molecular1
  • Advanced GPC/SEC analysis using multi-detectors is increasingly being used to measure absolute molecular weight independent of column retention volume and to add other measurements such as those of intrinsic viscosity and hydrodynamic radius. (pharmaceutical-technology.com)
Electrophoresis1
  • Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (lookformedical.com)