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  • mobile phases
  • Among the various mobile phases evaluated, [acetonitrile-methanol(64:36)]-[25 mM tris(hydroxymethyl)aminomethane/pH 95:5) eluent systems produced the most satisfactory results. (usda.gov)
  • sorbent
  • The stationary phase comes in the form of a packed syringe-shaped cartridge, a 96 well plate, a 47- or 90-mm flat disk, or a microextraction by packed sorbent (MEPS) device, a SPE method that uses a packed sorbent material in a liquid handling syringe. (wikipedia.org)
  • column
  • running approximately 20 column volumes of mobile phase is recommended. (thermofisher.com)
  • ILs are attractive stationary phase materials for GC, offering several benefits, including high polarity, high viscosity, and low vapour pressure as a result of their ionic nature, which helps to improve GC column coating. (chromatographyonline.com)
  • Avoiding the use of pressure to introduce the mobile phase into the column, results in a number of important advantages. (wikipedia.org)
  • A second advantage of using electroosmosis to pass the mobile phase into the column is the plug-like flow velocity profile of EOF, which reduces the solute dispersion in the column, increasing column efficiency. (wikipedia.org)
  • The pumps deliver the desired flow and composition of the mobile phase through the column. (wikipedia.org)
  • The velocity of each component depends on its chemical nature, on the nature of the stationary phase (column) and on the composition of the mobile phase. (wikipedia.org)
  • The method consists of a gaseous mobile phase, usually an inert carrier gas, such as helium, and a liquid stationary phase adsorbed onto an inert cylindrical solid support called a column. (selectscience.net)
  • A reversed phase Ace C8 (150 × 4.6 mm i.d., 5 μm) column was placed in the column oven at 25°C. (omicsonline.org)
  • An injector near the entrance to the column is used to add the analyte. (britannica.com)
  • The mobile phase flows through the packed bed or column. (britannica.com)
  • The ′mobile phase′ in GC, it transports analytes through the column, between the injector and detector, when the analytes are not partitioned into the stationary phase. (sigmaaldrich.com)
  • One mixed-mode column can replace two or even more single mode columns, which is economic and eco-friendly for employing the stationary phase more sufficiently and reducing the consuming and 'waste' of raw materials. (wikipedia.org)
  • Another approach is "biphasic column", by packing two stationary phases separately in two ends of the same column. (wikipedia.org)
  • For the dry method, the column is first filled with dry stationary phase powder, followed by the addition of mobile phase, which is flushed through the column until it is completely wet, and from this point is never allowed to run dry. (wikipedia.org)
  • For the wet method, a slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. (wikipedia.org)
  • The individual components are retained by the stationary phase differently and separate from each other while they are running at different speeds through the column with the eluent. (wikipedia.org)
  • The flow rate of such a column can be increased by extending the fresh eluent filled column above the top of the stationary phase or decreased by the tap controls. (wikipedia.org)
  • The eluate is the mobile phase leaving the column. (wikipedia.org)
  • The two liquid phases come in contact with each other as at least one phase is pumped through a column, a hollow tube or a series of chambers connected with channels, which contains both phases. (wikipedia.org)
  • More often, however, one liquid acts as the stationary phase and is retained in the column while the mobile phase is pumped through it. (wikipedia.org)
  • One or more pumps deliver the phases to the column which is the CCC instrument itself. (wikipedia.org)
  • This improved economic performance is brought about by a valve-and-column arrangement that is used to lengthen the stationary phase indefinitely and allow very high solute loadings to the process. (wikipedia.org)
  • The stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside a piece of glass or metal tubing called a column (an homage to the fractionating column used in distillation). (wikipedia.org)
  • In a GC analysis, a known volume of gaseous or liquid analyte is injected into the "entrance" (head) of the column, usually using a microsyringe (or, solid phase microextraction fibers, or a gas source switching system). (wikipedia.org)
  • elution
  • Fayyad MK, Misha AK, Yousef Al-Musaimi OI (2010) Effect of Temperature, Wavelength, pH, Ion Pair Reagents and Organic Modifiers' Concentration on the Elution of Cystatin C. Stability of Mobile Phase. (omicsonline.org)
  • ionizable
  • Further experimental verification of chaotropicity theory and its applicability to the description of chromatographic behavior of wide range of ionizable analytes suggests the more complex mechanism than simple solvation desolvation equilibrium as it was originally thought. (shu.edu)