In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)
To date, three major classes of Ca 2+ -binding proteins, including calcium dependent protein kinases (CDPK), calmodulins (CaM) and CaM-like proteins (CaML) and calcineurin B-like proteins (CBL), have been characterized in higher plants [ 6 , 7 ]. (biomedcentral.com)
Protein11
KCa2.2 is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. (wikipedia.org)
Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. (tamu.edu)
Characterizing the roles of Arabidopsis calmodulin-like protein, CML39, in hormonal regulation of early seedling development and fruit formation. (weebly.com)
Our chemical-shift based secondary structure analysis reveals the human DBNDD1 as an intrinsically disordered protein. (springer.com)
We performed a Basic Local Alignment Search Tool (BLAST) analysis to identify regions of local similarity between the human DBNDD1 and protein sequences from other species (Fig. 1 ). (springer.com)
In the analysis of subcellular localization,we found BoPID protein localized in the cell membrane and cytoplasm,indicating that it might be an amphibious protein and anchored on the membrane and synapses in the cytoplasm. (ahs.ac.cn)
The second is a signaling role: assembles signaling molecules including neuronal nitric oxide synthase (nNos) growth factor receptor-bound protein 2 (Grb2) Calmodulin and AG-014699 Calmodulin-dependent kinases (Anderson et al. (researchassistantresume.com)
This increased host vulnerability at higher temperatures is due to downregulated expression of CALMODULIN BINDING PROTEIN 60-LIKE G (CBP60g) and SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1), which encode master regulators of SA production. (wlu.ca)
Protein kinase D1 (PKD1), together with PKD2 and PKD3, constitute a family classified within the calcium/calmodulin-dependent protein kinase superfamily 7 . (nature.com)
I have a lot of experience in the following methodologies: protein isolation, protein synthesis assays, Western blotting, genotyping, RNA isolation and qPCR miRNA/mRNA arrays, bioinformatics and statistical analyses. (otago.ac.nz)
Although biochemical and biophysical assays and cell biology experiments advance these insights, full elucidation of the pathophysiology caused by human disease requires a broader approach, including assessment of the impact that cells with a pathogenic mutation have on neighboring cells that do not express the mutant protein and consideration of system-wide responses to a diseased organ. (rupress.org)
Phenotype3
We found that deletion of NUP2 delayed pairing and synapsis during meiosis, and led to decreased spore viability, similar to the ndj1Δ mutant phenotype. (biorxiv.org)
Mutant phenotype analyses have demonstrated that the knock-out mutation of different key genes in the symbiotic signaling pathway block AM-associated endoreduplication. (biomedcentral.com)
Hence, the novel surrogate myristoyl moiety not only failed to increase NOS2 activity when introduced in a wild-type sequence environment, but was also unable to rescue the inactive phenotype of the Cys3Ser mutant. (biologists.com)
However, their calcium-binding domain is restricted by calmodulin from metazoans. (mdpi.com)
In this study, we developed red GECI, called FRCaMP, using calmodulin (CaM) from Schizosaccharomyces pombe fungus as a calcium binding domain. (mdpi.com)
Gene7
Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)
Indirect confirmation that ploidy increases are part of the AM accommodation program comes from gene expression increases in all major endoreduplication markers (such as MtCCS52A and MtAPC subunit 2 ) and from the analysis of mutant plant lines that cannot be colonized by AM fungi, where no change in nuclear size and ploidy are recorded [ 19 ]. (biomedcentral.com)
Here, we present a genome-wide analysis of the CDPK gene family in maize for the first time, and this genomic analysis of maize CDPK genes provides the first step towards a functional study of this gene family in maize. (biomedcentral.com)
While many studies have focused on structural-functional analysis of in skeletal muscle mass to develop gene therapy [examined by (Blankinship et al. (researchassistantresume.com)
The introduction of a normal, or wild type, copy of the proposed gene - referred to as BRUSH - into the brush mutant did not correct the problems with its roots. (elifesciences.org)
Finally, I performed gene expression analysis of CBP60g and SARD1, and determined that the different temperature-resilient accessions showed varied gene expression profiles that did not always correspond with their pathogen resilience. (wlu.ca)
The powerful high throughput screening approach, developed in my group, enables me to study all the possible combinations between a phenotypic reporter of choice and a collection of gene mutants throughout the yeast genome in a few months (while a conventional manual test would take more than several decades). (gu.se)
Genes4
Sequence analysis suggests that a 36-amino acid N-terminal regulatory domain may be present in all Arabidopsis CAX-like genes. (manchester.ac.uk)
overexpression similarly decreased the manifestation of several Crz1p target genes as determined by Northern analysis (data not demonstrated). (gasyblog.com)
In this study, we performed a bioinformatics analysis of the entire maize genome and identified 40 CDPK genes. (biomedcentral.com)
Expression analyses suggested that maize CDPK genes are important components of maize development and multiple transduction pathways. (biomedcentral.com)
Viability2
gem homozygous receptor mutant HCs display normal cell viability, afferent synaptogenesis, and peripheral innervation, yet exhibit strongly reduced extracellular potentials (∼50% of wild-type potentials). (jneurosci.org)
We found that both mutations were unable to fully rescue the viability of the respective null mutants of the Drosophila homologs, hts and Gcn5, suggesting that they are indeed pathogenic in flies. (nih.gov)
Kinase2
mutants suggest that the kinase is important in many essential cell functions. (gasyblog.com)
BoPID may have kinase activity based on the kinase phosphorylation prediction analysis of its sequence,its sequence is highly homologous with AtPID at the chromosome level and has strong species specificity when clustering with cruciferous plants such as turnip, Arabidopsis and Brassica napus in the phylogeny and collinearity analysis. (ahs.ac.cn)
Phenotypes1
Overexpression analyses and rescue experiments of Cobl loss-of-function phenotypes with Cobl mutants in primary neurons and in tissue SNX-2112 slices demonstrated the importance of CaM binding for Cobl's functions. (healthanddietblog.info)
Abnormalities1
Our ongoing phenotypic analysis of cml39 knock-out plants has identified several additional developmental abnormalities in these mutants. (weebly.com)
Phenotypic1
Here, we present preliminary data for the delineation of the interaction domain along with the phenotypic analysis of KO mutants of CML39IP. (weebly.com)
Rescue1
While the KAT2B/Gcn5 mutation additionally showed a significantly reduced ability to rescue morphological and functional defects of cardiomyocytes and nephrocytes (podocyte-like cells), this was not the case for the ADD3 mutant rescue. (nih.gov)
Yeast1
Analysis of a full-length CAX1 clone suggested that the CAX1 open reading frame contains an additional 36 amino acids at the N terminus that were not found in the original clone identified by suppression of yeast (Saccharomyces cerevisiae) vacuolar Ca2+ transport mutants. (manchester.ac.uk)
Calcineurin2
nevertheless inhibition of calcineurin does not suppress the development flaws of mutant cells possess cytokinesis flaws this observation Ki16425 shows that Hrr25p may play a significant function in cell parting. (gasyblog.com)
The calmodulin-binding transcription activators (CAMTA) play critical roles in plant growth and responses to environmental stimuli. (frontiersin.org)
Wild type6
Partners of wild type Grb7 and a mutant lacking its calmodulin-binding domain. (nih.gov)
Mice (n = 8 mice per group) fed an HFD were inoculated by gavage with 1:1 mixtures of the wild-type (WT) and either hap43Δ/Δ mutant or HAP43 reintegrant ( HAP43 AB) cells (1 × 10 8 CFU per mice). (elifesciences.org)
During the course of experiments, mice (n = 6 mice per group) fed HFD were received drinking water supplemented with or without NAC (1.5 g/L) and then inoculated by gavage with 1:1 mixtures of the wild-type (WT) and hap43Δ/Δ mutant cells (1 × 10 8 CFU per mice). (elifesciences.org)
Mice (n = 8) fed a normal Fe diet (NFD) were inoculated by gavage with 1:1 mixtures of the wild-type (WT) and hap43Δ/Δ mutant cells (1 × 10 8 CFU per mice). (elifesciences.org)
In the absence of palmitoylation, introduction of a surrogate myristoylation site resulted in a mutant NOS2 with only 25% activity compared with the wild-type enzyme. (biologists.com)
The open and filled lozenges indicate the locations of the wild-type and mutant amino acids at position 769, respectively. (2medicalcare.com)
Nuclear1
We here compare the efficiency of three Fiji/ImageJ image analysis plugins in localizing and quantifying the increase in nuclear size - a hallmark of recursive events of endoreduplication - in M. truncatula roots colonized by the AM fungus Gigaspora margarita . (biomedcentral.com)
Cells3
In conclusion, our results indicate 3D Object Counter as the best performing Fiji/ImageJ image analysis script in plant root thick sections and its application highlighted endoreduplication as a major feature of the AM pre-penetration response in root cortical cells. (biomedcentral.com)
mutant cells noticed 5 h following the addition of blood sugar to KKY387. (irjs.info)
The calmodulin-like domain contains EF-hands for Ca 2+ binding. (biomedcentral.com)
However Dp116 expression in mouse mutants lacking both and Utrophin ('signaling domain name' in the absence of the 'mechanical domain name' function. (researchassistantresume.com)
Quantitative1
C ) Quantitative analysis using fluorescence intensity of DHE ( a ) in the colon. (elifesciences.org)
Arrays1
Previous studies have identified one mutant version of this plant, called brush , which develops abnormal roots with brush-like arrays of root hairs near the tip. (elifesciences.org)
Expression analysis2
The spatiotemporal expression analysis indicated that BoPID expressed higher in the stigma than that in other parts of floral organs,while it was up-regulated after 15 minutes of self-pollination and then down-regulated. (ahs.ac.cn)
Cloning and Expression Analysis of BoPID in Brassica oleracea [J]. Acta Horticulturae Sinica, 2021, 48(6): 1123-1134. (ahs.ac.cn)
Display3
CML39 knock-out (KO) mutants display developmental arrest in the absence of exogenous sucrose. (weebly.com)
This fusion complemented an (mutants are either inviable or display a severe development defect (Hoekstra et al. (gasyblog.com)
With regards to the stress history, mutants are either inviable or display a severe development defect (Hoekstra et al. (irjs.info)
Roots1
Further analysis revealed that it was actually the ratio between BRUSH and brush expression levels that was critical for determining how the plant's roots developed. (elifesciences.org)
The brush mutant was also unable to form a symbiosis with rhizobia, and structures that allow the bacteria to enter the plant stopped developing before they were complete. (elifesciences.org)
Diagnostic2
Furthermore, receiver operating characteristic (ROC) curve analysis was conducted to determine diagnostic efficiency, and a nomogram was developed to predict HF. (bvsalud.org)
MicroRNAs in Parkinson's disease: A systematic review and diagnostic accuracy meta-analysis. (otago.ac.nz)
Significantly2
H ) Treatment of the antioxidant N-acetyl-L-cysteine (NAC) is able to partially but significantly abrogate the commensal fitness of Hap43 mutant in HFD-fed mice. (elifesciences.org)
Kaplan-Meier survivorship analysis at twenty years revealed significantly lower survival rates for cemented implants as compared With cementless implants. (ilreceptor.com)
Survival1
Survival analysis showed a higher mortality risk in females with higher CLR levels (P (bvsalud.org)
Suggest1
Our findings of significant linkage of the alpha-adducin locus to essential hypertension and greater sensitivity to changes in sodium balance among patients with the mutant allele suggest that alpha-adducin is associated with a salt-sensitive form of essential hypertension. (nih.gov)
Patients1
A total of 913 patients were included in the analysis (611 males and 302 females) from October 2012 to May 2020, and data on demographic characteristics, blood biochemical markers, and cardiac ultrasound results were collected. (bvsalud.org)
Present1
2008 The as well as the Dystroglycan mutants had been a generous present from R. Ray. (researchassistantresume.com)
Stress1
In contrast, expression of an active dephosphorylation-resistant PKD1 mutant potentiates the IKK/NF-κB/SOD2 oxidative stress detoxification pathway and confers neuroprotection from in vitro and in vivo excitotoxicity. (nature.com)