Quadrupole Tandem Mass SpectroChromatography-tandem mass spectroElectrospray Ionization MProteomicsLiquidSpectrometerProteinsProteomicHPLCSubstancesQuantitationMetabolitesMetabolismSequence AnalysisSpecificityQuantificationSpectroscopyApplicabilityIdentificationSpectraSpectromSpectrometric analysisFragmentationForensicPharmacologyInstrumentationDataConcentrationsMethodIonsSeparationAromaticMultiple reactionMethodsSensitivitySpectrumExperimentalReactionPathwayAccuracyResults
Quadrupole Tandem Mass Spectro2
- In this study, the qualitative identification of 34 designer drugs, mainly synthetic cannabinoids and synthetic cathinones, was performed by gas chromatography-triple quadrupole-tandem mass spectrometry with two different ionization techniques, including electron ionization (EI) and chemical ionization (CI), focusing only on qualitative seized-drug analysis, not from the toxicological point of view. (ojp.gov)
- A sensitive, rapid, selective and accurate liquid chromatography coupled to quadrupole tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous identification and quantification of tamsulosin and finasteride in bulk and in their combined dosage form. (eurjchem.com)
Chromatography-tandem mass spectro10
- A fast and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination and pharmacokinetic study of nimodipine in human plasma. (unboundmedicine.com)
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements for PRA and PAC have become popular. (degruyter.com)
- Moreover, we analyzed changes in transcriptomics using RNA sequencing and metabolomics using gas chromatography-tandem mass spectrometry (GC-MS/MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) in PM 2.5 -treated HL-1 cells. (nih.gov)
- Therefore, we developed and validated a liquid chromatography-tandem mass spectrometry method to simultaneously analyze immunosuppressive drugs such as mycophenolic acid, antifungal drugs such as voriconazole and itraconazole, and its metabolite hydroxyitraconazole. (elsevierpure.com)
- Samples were analyzed for perchlorate, hiocyanate and nitrate using ion chromatography tandem mass spectrometry (Valentin-Blasini et al. (cdc.gov)
- Here a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous analyses of multiple steroid hormones in cetacean blubber is validated and applied to 10 stranded humpback whales (Megaptera novaeangliae). (nist.gov)
- 2011): Optimization, In-House Validation, and Application of a Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-Based Method for the Quantification of Selected Polyphenolic Compounds in Leaves of Grapevine (Vitis vinifera L. (boku.ac.at)
- The aim of the presented work was to develop and optimize a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to study the pattern and amount of selected polyphenols in leaves of Vitis vinifera L. The method is simple and does not require any sample cleanup. (boku.ac.at)
- Determination of 16 Per and Polyfluoroalkyl Substances (PFAS) in Food using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) (Version 2021). (fda.gov)
- In total, 791 annotated endogenous metabolites were measured by ultra-performance liquid chromatography-tandem mass spectrometry. (lu.se)
Electrospray Ionization M1
- A novel sensitive method utilizing nano extractive electrospray ionization mass spectrometry (nanoEESI-MS) has been developed for the rapid analysis of aerosol drug samples with quantitative information. (elsevierpure.com)
Proteomics2
- However, current proteomics workflows entail several manual sample preparation steps and are challenged by the microscopic mass-limited samples generated by LCM, impacting measurement robustness, quantification and throughput. (nih.gov)
- Here, we coupled LCM with a proteomics workflow that provides fully automated analysis of proteomes from microdissected tissues. (nih.gov)
Liquid3
- Mass spectrometry coupled to liquid chromatography is a powerful tool for the analysis of complex plant derivatives such as GADF. (unboundmedicine.com)
- We use a combination of MS techniques, namely liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) on a triple quadrupole, for the identification of the polyphenolic constituents of the soluble fraction of GADF. (unboundmedicine.com)
- Furthermore, with the most commonly used chemometrics algorithms, it is difficult to accurately extract and discriminate the huge amounts of data generated by mass spectrometry, such as ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF). (hindawi.com)
Spectrometer12
- The molecules of a given sample are ionized and the first spectrometer (designated MS1) separates these ions by their mass-to-charge ratio (often given as m/z or m/Q). Ions of a particular m/z-ratio coming from MS1 are selected and then made to split into smaller fragment ions, e.g. by collision-induced dissociation, ion-molecule reaction, or photodissociation. (wikipedia.org)
- These fragments are then introduced into the second mass spectrometer (MS2), which in turn separates the fragments by their m/z-ratio and detects them. (wikipedia.org)
- Typical tandem mass spectrometry instrumentation setups include triple quadrupole mass spectrometers (QqQ), multi-sector mass spectrometer, quadrupole-time of flight (Q-TOF), Fourier transform ion cyclotron resonance mass spectrometers, and hybrid mass spectrometers. (wikipedia.org)
- Q-TOF mass spectrometer combines TOF and quadrupole instruments, which cause high mass accuracy for product ions, accurate quantitation capability, and fragmentation experiment applicability. (wikipedia.org)
- Hybrid mass spectrometer consists of more than two mass analyzers. (wikipedia.org)
- Multiple stages of mass analysis separation can be accomplished with individual mass spectrometer elements separated in space or using a single mass spectrometer with the MS steps separated in time. (wikipedia.org)
- and BEBE - four-sector (reverse geometry) mass spectrometer. (wikipedia.org)
- The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. (unboundmedicine.com)
- The analyte ions of interest were guided into an ion trap mass spectrometer for tandem mass analysis. (elsevierpure.com)
- Finally, most of the identities were unequivocally confirmed by accurate mass measurements on the TOF spectrometer. (unboundmedicine.com)
- MS/MS detection was carried out by monitoring the fragmentation of 408.74 → 227.29 ( m/z ), 373.11 → 304.96 ( m/z ) and 255.75 → 166.15 ( m/z ) for tamsulosin, finasteride and diphenhydramine (IS), respectively, on a triple quadrupole mass spectrometer. (eurjchem.com)
- The eluant from the column is ionized using an electrospray interface to generate and transmit negative ions into the mass spectrometer. (cdc.gov)
Proteins10
- A set of tools for detecting differentially abundant peptides and proteins in shotgun mass spectrometry-based proteomic experiments with tandem mass tag (TMT) labeling. (bioconductor.org)
- A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides. (wikipedia.org)
- While progress has been made in establishing a renal cortical proteome using 1-D or 2-DE and mass spectrometry, the number of proteins definitively identified by mass spectrometry has remained surprisingly small. (biomedcentral.com)
- Low coverage of the renal cortical proteome as well as our interest in diabetes-induced changes in proteins found in the renal cortex prompted us to perform an in-depth proteomic analysis of mouse renal cortical tissue. (biomedcentral.com)
- Many of these proteins, such as low-abundance proteins, membrane proteins and proteins with extreme values in pI or MW are traditionally under-represented in 2-DE-based proteomic analysis. (biomedcentral.com)
- Based on immunological and mass spectrometric analysis, SALSA was estimated to constitute up to 4-10% of the total protein amount in meconium, making it one of the most abundant proteins. (researchgate.net)
- Although mass spectrometry (MS) can be used to quantify proteins in a complex mixture, the quantitative information on the peptide-level is sparse. (aau.dk)
- A database was constructed with the developed tool, and it was used to identify caspase target proteins from tandem mass spectrometry data from two different proteomic experiments. (biomedcentral.com)
- 140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry. (lu.se)
- Many proteins with ill-defined functions were identified as well as a number of proteins that at the time of the analysis were described only as ORFs. (lu.se)
Proteomic1
- The possibility to create a database from predicted caspase cleavage products for the whole genome could significantly aid in identifying novel caspase targets from tandem mass spectrometry based proteomic experiments. (biomedcentral.com)
HPLC3
- We report a large scale analysis of mouse renal cortical proteome using SCX prefractionation strategy combined with HPLC - tandem mass spectrometry. (biomedcentral.com)
- This Special Issue aims to create a forum of discussion on novel spectral analysis methods, like UV-vis, IR, Raman, NMR, MS, HPLC-DAD, and HPLC-MS, combining chemometrics algorithms for application to food quality and safety. (hindawi.com)
- Title : Evaluation of an Isotope Dilution HPLC Tandem Mass Spectrometry Candidate Reference Measurement Procedure for Total 17-β Estradiol in Human Serum Personal Author(s) : Botelho, Julianne Cook;Ribera, Ashley;Cooper, Hans C.;Vesper, Hubert W. (cdc.gov)
Substances1
- Due to the significant detection interference caused by the complex food matrix, false negative results caused by trace amounts of harmful substances, and the failure of targeted detection caused by endless adulteration, the specificity, and sensitivity of spectrum analysis need to be further improved. (hindawi.com)
Quantitation1
- performs statistical analysis steps, that follow peptide identification and quantitation. (bioconductor.org)
Metabolites1
- They extracted deoxyribonucleic acid (DNA) for metagenomic sequencing and metabolites for untargeted tandem mass spectrometry (MS/MS) analysis. (news-medical.net)
Metabolism2
- Pathway enrichment analysis highlighted branch-chained and aromatic amino acid synthesis/metabolism, aminoacyl-tRNA biosynthesis, and sphingolipid metabolism. (lu.se)
- This meta-analysis aimed to evaluate current human epidemiological evidence on the relationship between PFAS exposure and glucolipid metabolism in childhood and adolescence. (bvsalud.org)
Sequence Analysis1
- Ravi K, García-Hidalgo J, Brink DP, Skyvell M, Gorwa-Grauslund MF, Lidén G. Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium. (lu.se)
Specificity2
- In recent years, chemometrics have been widely used in spectral analysis to increase the specificity and intelligence of the analysis. (hindawi.com)
- The specificity of most spectrum analysis is only targeted at a very limited number of reaction groups of compounds. (hindawi.com)
Quantification1
- This experiment is commonly performed to identify transitions used for quantification by tandem MS. In a neutral loss scan, the first mass analyzer scans all the masses. (wikipedia.org)
Spectroscopy1
- The molecular mass of SARS-CoV-2 M pro as determined by mass spectroscopy is 33797.0 Da, consistent with its theoretical molecular mass of 33796.8 Da. (nature.com)
Applicability1
- The applicability of tandem mass spectrometry to the quality analysis of metal-surface protect surfactants without any pretreatment or purification of the sample solution was investigated. (elsevierpure.com)
Identification5
- The implementation of CI source facilitates the determination of molecular mass and the identification of seized designer drugs. (ojp.gov)
- Each instrumental configuration utilizes a unique mode of mass identification. (wikipedia.org)
- Tandem mass spectrometry was used to confirm the identification of important compounds in each aerosol drug sample. (elsevierpure.com)
- As technology develops, spectrum analysis has become the main method for the analysis and identification of compounds. (hindawi.com)
- Detection and Identification of Explosives by Mass Spectrometry (D.D. Fetterolf). (routledge.com)
Spectra5
- Currently, the qualitative seized-drug analysis is mainly performed by gas chromatography-electron ionization-mass spectrometry (GC-EI-MS) in which most of these emerging designer drug derivatives are extensively fragmented, not presenting a molecular ion in their mass spectra. (ojp.gov)
- In addition, CI mass spectra and MRM mass spectra of these designer drug derivatives can be used as a potential supplemental database along with EI mass spectral database. (ojp.gov)
- Recently, tandem mass spectrometry (MS/MS) based approaches seemed to be reaching the limit on the amount of information that could be extracted from MS/MS spectra. (nih.gov)
- However, a closer look reveals that a common limiting procedure is to analyze each spectrum in isolation,even though high throughput mass spectrometry regularly generates spectra from related peptides. (nih.gov)
- Spectral networks algorithms for de novo interpretation of tandem mass spectra / Nuno Bandeira. (nih.gov)
Spectrom1
- Rapid Commun Mass Spectrom 2012 Jul 15;26(13):1533-40. (cdc.gov)
Spectrometric analysis1
- Forensic Drug Testing by Mass Spectrometric Analysis of Hair (W.A. Baumgartner, C.-C. Cheng, T.D. Donahue, G.F. Hayes, V.A. Hill, and H. Scholtz). (routledge.com)
Fragmentation3
- The fragmentation step makes it possible to identify and separate ions that have very similar m/z-ratios in regular mass spectrometers. (wikipedia.org)
- This is a method of mass spectrometry that ion fragmentation (m/z) ratio determined through a time of flight measurement. (wikipedia.org)
- fragmentation method that can be performed on mass spectrometry platforms capable of ion-ion reactions. (casss.org)
Forensic5
- Uhl M. "Tandem mass spectrometry: a helpful tool in hair analysis for the forensic expert" Forensic Sci Int . 2000;107(1-3):169-79. (erowid.org)
- An unsurpassed combination for rendering an expert opinion based on hair analysis may be: a forensic expert using diligence and experience, coupled with the performance of a sophisticated analytical instrument. (erowid.org)
- Forensic Applications of Mass Spectrometry combines the most current developments in applications of mass spectrometry techniques to forensic analyses. (routledge.com)
- Forensic Applications of Pyrolysis-Mass Spectrometry (T.O. Munson). (routledge.com)
- Use of Isotope Ratios in Forensic Analysis (J.L. Brazier). (routledge.com)
Pharmacology1
- Our data demonstrate that nanoEESI tandem mass spectrometry is a fast and sensitive technique for the analysis of aerosol drug preparations, showing promising applications in pharmacology studies and in situ analysis of aerosol drugs on the market. (elsevierpure.com)
Instrumentation1
- The instrumentation used for hair analysis is a powerful analytical tool: a Varian 3400 gas chromatograph linked to a Finnigan Tandem-MS (TSQ 700). (erowid.org)
Data5
- To do this, we designed and synthesized the fluorescently labelled substrate Mca-AVLQ↓SGFRK(Dnp)K, derived from the N-terminal autocleavage sequence of the viral protease, for time-dependent kinetic analysis (Extended Data Fig. 1e ). (nature.com)
- developed to aid data analysis throughput of structural assignments. (casss.org)
- Human cancer genome sequencing studies have generated ample, publicly available data, and analyses of these data have substantially broadened the knowledge of somatic mutations accumulating in tumours. (who.int)
- METHODS: Data from the National Health and Nutrition Examination Survey (NHANES) between 2005 and 2008 were utilized for this cross-sectional analysis. (bvsalud.org)
- DATA COLLECTION AND ANALYSIS: Two review authors independently screened the titles and abstracts of the records recovered by the search, read through the full-text articles of potentially eligible studies, and extracted data. (cdc.gov)
Concentrations1
- In the noncompartmental analysis, peak triamcinolone concentrations of 5.4 ng/mL were detected within 22.1 hours after administration. (hindawi.com)
Method3
- The authors concluded that FAB CID-MS/MS will be a good analytical method for direct quality analysis without any pretreatment or purification of the metal-surface protect surfactant for industrial use. (elsevierpure.com)
- The simplicity and sensitivity of this method allows its use in the quality control of the cited drugs and can be extended for routine analysis of the drugs in their pharmaceutical preparations. (eurjchem.com)
- The method is applicable to the determination of perchlorate in 0.05 μg/L of urine over the range 0.005 to 1000 μg/L. The limit of detection for thiocynate was 20 μg/L and for nitrate 700 μg/L . Stable isotope labeled internal standards were employed in the analysis of perchlorate, thiocyanate and nitrate. (cdc.gov)
Ions1
- By doing tandem mass spectrometry in time, the separation is accomplished with ions trapped in the same place, with multiple separation steps taking place over time. (wikipedia.org)
Separation2
- In tandem mass spectrometry in space, the separation elements are physically separated and distinct, although there is a physical connection between the elements to maintain high vacuum. (wikipedia.org)
- Li K, Al-Rudainy B, Sun M, Wallberg O, Hulteberg C, and Tunå P. Membrane Separation of the Base-Catalyzed Depolymerization of Black Liquor Retentate for Low-Molecular-Mass Compound Production. (lu.se)
Aromatic1
- Comparative analysis of aromatic diisocyanate conjugation to human albumin utilizing multiplexed tandem mass spectrometry. (cdc.gov)
Multiple reaction1
- Developed multiple reaction monitoring (MRM) mode may increase sensitivity and selectivity in the analysis of seized designer drugs. (ojp.gov)
Methods2
- There is a need for analytical techniques and methods for accurate, low level analysis of Ti in environmental samples, biological tissues, and other samples. (spectroscopyonline.com)
- Reaction gas methods can use either on-mass or mass-shift measurement. (spectroscopyonline.com)
Sensitivity1
- A sensitivity analysis was used to evaluate the robustness and reliability of the combined results. (bvsalud.org)
Spectrum2
- Tandem in time MS instruments do not use the modes described next, but typically collect all of the information from a precursor ion scan and a parent ion scan of the entire spectrum. (wikipedia.org)
- Tandem MS in space uses the coupling of two instrument components which measure the same mass spectrum range but with a controlled fractionation between them in space, while tandem MS in time involves the use of an ion trap. (wikipedia.org)
Experimental1
- There are a number of different tandem MS/MS experimental setups and each mode has its own applications and provides different information. (wikipedia.org)
Reaction1
- Tandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. (wikipedia.org)
Pathway1
- Using bioinformatic analysis with ingenuity pathway analysis (IPA) software, we found that the changes in the transcriptome and metabolome are highly related to changes in biological functions, including homeostasis of Ca 2+ , depolarization of mitochondria, the function of mitochondria, synthesis of ATP, and cardiomyopathy. (nih.gov)
Accuracy1
- We evaluate the accuracy of these methodologies to grasp the global dynamics of phosphorylation, and we raise awareness on ambiguities inherent to these analyses. (acs.org)
Results2
- Results: A total of 12 studies were included in this analysis. (bvsalud.org)
- We presented the results by study design and combined clinically homogeneous studies in meta-analyses using random-effects models. (cdc.gov)