• hsa-miR-221 and hsa-miR-124 promoted and hsa-miR-140-5p inhibited neuronal differentiation, hsa-miR-26a-5p promoted and hsa-miR-424 suppressed angiogenic differentiation, and hsa-miR-135 and hsa-miR-143 inhibited differentiation within myogenic lineages. (biomedcentral.com)
  • In addition to these intermediate transcriptional regulators, we show that atonal directly regulates a novel gene, dilatory, that is directly associated with ciliogenesis during neuronal differentiation. (tcd.ie)
  • Region-spe-cific quantification of IHC labeling showed that IGF-1 treatment promoted neuronal differentiation, increased subcorti-cal myelination, and attenuated synaptogenesis in a region-dependent and time-dependent manner. (lu.se)
  • Monocot leaves, where cell proliferation and differentiation are displayed along a linear developmental gradient, provide an ideally suited experimental system to study these processes. (biomedcentral.com)
  • It is not dissimilar to the gradient of proliferation and differentiation along developing roots [ 2 ] but, in contrast, it provides a unique opportunity to study chloroplast biogenesis and differentiation. (biomedcentral.com)
  • Recent studies show that the human parathyroid calcium sensing receptor (CaSR) is expressed in human colon epithelium and functions to regulate epithelial proliferation and differentiation. (aacrjournals.org)
  • Both agents concomitantly induced a series of changes in the CBS cells that influence proliferation and differentiation, but cellular responses to the two agents were not identical. (aacrjournals.org)
  • It has been reported that TGF-β influence different cell functions, including growth, proliferation and differentiation. (biomedcentral.com)
  • A specialized type of differentiation, known as terminal differentiation, is of importance in some tissues, including vertebrate nervous system, striated muscle, epidermis and gut. (wikipedia.org)
  • During terminal differentiation, a precursor cell formerly capable of cell division permanently leaves the cell cycle, dismantles the cell cycle machinery and often expresses a range of genes characteristic of the cell's final function (e.g. myosin and actin for a muscle cell). (wikipedia.org)
  • Differentiation may continue to occur after terminal differentiation if the capacity and functions of the cell undergo further changes. (wikipedia.org)
  • In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)
  • Among these, the neoplastic tumor-suppressor genes (nTSGs) have been shown to fulfill two significant functions requisite for organ size control: first, establishing apicobasal cell polarity as a principle of epithelial tissue organization and appropriate timing of terminal differentiation and, second, exerting cell-proliferation control as a primary factor in tissue growth. (fsu.edu)
  • Serial analysis of gene expression identified genes involved in cellular differentiation/structure (cytokeratin-8 and -18) and growth/cell migration/mesoderm-epithelial interaction (stratifin and midkine), which could potentially be regulated by steroids like estrogen. (usda.gov)
  • It is concluded that CaSR may function in the colon to regulate epithelial differentiation and that loss of CaSR expression may be associated with abnormal differentiation and/or malignant progression. (aacrjournals.org)
  • The MAP score is enriched for genes involved in epithelial de-differentiation, cellular division, and the tumor microenvironment. (vanderbilt.edu)
  • The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
  • However, the full promise of regenerative medicine has been difficult to achieve so far, partly due to our incomplete understanding of the genetic and epigenetic mechanisms regulating differentiation of stem cells to specific lineages and tissues. (confex.com)
  • It also suggests a model for how subtype differentiation in different neuronal lineages may be regulated by different proneural factors. (tcd.ie)
  • Exosomes are a non-cellular resource and therefore free from the potential to differentiate into unintended cellular lineages, which is a major problem of MSC transplantation [ 18 ]. (biomedcentral.com)
  • miRNAs involved in odontogenic/osteogenic differentiation were then included in a bioinformatic analysis. (biomedcentral.com)
  • Thirteen miRNAs promoted and 17 suppressed odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • hsa-miR-140-5p, hsa-miR-218 and hsa-miR-143 were more frequently reported suppressing the odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • KEGG pathway analysis revealed MAPK, PI3K-Akt and FoxO as key signaling pathways involved in the odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • Molecular analysis of melanocytes and melanoma cells from mouse models: transcriptomics, bioinformatics, proteomics. (ubc.ca)
  • Dr. Maity obtained his Ph.D. in Cellular and Molecular Biology from the Life Science and Biotechnology department at Jadavpur University, India in 2017, where he studied the DNA damage repair protein WRN and autophagy. (nih.gov)
  • Dr. Jyoitirindra Maity has focused on cellular and molecular biology in rare disease models and cancer and stem cell modeling throughout his career. (nih.gov)
  • In summary, we deciphered the cellular heterogeneity and the molecular dynamics during fibrocartilage differentiation. (elifesciences.org)
  • CONCLUSION: The butyrate-induced responses of the U138B cell line provide a convenient model system for studying the molecular events accompanying the differentiation of glioblastoma cells. (northwestern.edu)
  • Taken together, our data suggest that Mahjong and Lgl belong to a molecular pathway that plays an evolutionarily conserved role in regulating cellular competitiveness. (fsu.edu)
  • Through the study of temporal and spatial regulation of important genes and pathways in our model systems, we will develop a more comprehensive picture of the molecular mechanisms orchestrating egg development and gain a deeper understanding of how basic cellular functions such as proliferation, differentiation, and growth are regulated in development. (fsu.edu)
  • Molecular analysis has demonstrated a mutation detection rate of as much as 56% in the CBP gene in patients with Rubinstein-Taybi syndrome. (medscape.com)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • Here, we report that adipocytes from tumor-invasive mammary fat undergo de-differentiation to fibroblast-like precursor cells during tumor progression and integrate into the tumor microenvironment. (ghenry.info)
  • Inducing de-differentiation by Xbp1s overexpression promotes tumor progression despite lower adiposity. (ghenry.info)
  • This protein is involved in different signaling pathways and in basic cellular functions, such as DNA repair, cell growth, differentiation, apoptosis, and tumor suppression. (medscape.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • We will also shortly discuss how leveraging single cell multi-omic approaches can be used to accelerate cellular biomarker discovery during clinical trials to predict response to therapy, follow responsive cell types, and define novel druggable target pathways. (frontiersin.org)
  • These data demonstrate that Akt2 expression is activated during cellular differentiation and suggest that it functions in the signaling pathways of some adult tissues. (nih.gov)
  • Results show lambs administered lipopolysaccharide treatments exhibited greater oxidative potential, as indicated by increased rectal temperatures, and upregulated expression of mRNA protein pathways essential for cellular differentiation, proliferation, and apoptotic events. (unl.edu)
  • Additionally, bioinformatic analyses were conducted to discover target genes, signaling pathways and gene ontologies associated with the identified miRNAs. (biomedcentral.com)
  • Whilst the role of proneural factors in fate specification is well studied, the link between neural specification and the cellular pathways that ultimately must be activated to construct specialised neurons is usually obscure. (tcd.ie)
  • The CSF-1 receptor triggers signaling pathways that control many important cellular processes, such as cell growth and division (proliferation) and maturation of the cell to take on specific functions (differentiation). (medlineplus.gov)
  • Bioinformatic analysis of the proteomes revealed shared Gene Ontology (GO) terms and signaling pathways within the clusters of co-expressed proteins (Tables 1-3 and S4. (lu.se)
  • Thus, my research interests are to develop novel single-cell genomics methods to better understand how changes in the epigenetic landscape during normal development regulates cellular differentiation, information that is critical towards realizing the full potential of regenerative medicine. (confex.com)
  • Further Chromatin immunoprecipitation (ChIP) analysis confirmed that the KLF2 and active epigenetic marks (H3K27Ac and H3K4me3) were upregulated in the promoter region ATG7 during OB differentiation. (nih.gov)
  • A growing body of evidence shows that lncRNAs play important functional roles in diverse biological processes, such as epigenetic regulation, cell cycle regulation, cellular growth and differentiation, by regulating the level of target genes [ 3 , 4 ]. (biomedcentral.com)
  • Astrocytes have essential functions in brain homeostasis that are established late in differentiation, but the mechanisms underlying the functional maturation of astrocytes are not well understood. (nature.com)
  • The cellular maturation process, called differentiation, has long been thought to be affected by methylation. (bwfund.org)
  • To view the results, click on 2D-DIGE analysis of B cell maturation. (lu.se)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • Following this, the differentially expressed genes (DEGs) were included in Gene Ontology enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, protein‑protein interaction network and survival analyses. (cancerindex.org)
  • Therefore, a metabolic shift from mitochondrial respiration to the glycolytic pathway occurs during OB differentiation. (nih.gov)
  • A miRNA-mRNA network was constructed, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. (biomedcentral.com)
  • We determine that atonal activates this cellular differentiation pathway in several ways. (tcd.ie)
  • Bifurcation and sensitivity analyses show that external signaling cues are necessary for macrophage commitment and emergence to a phenotype, but that the intrinsic macrophage metabolism is equally important. (biorxiv.org)
  • During the cellular differentiation process, cellular metabolism alters as the cell prepares for ongoing changes. (nih.gov)
  • Innate immune activation and differentiation is accompanied by comprehensive changes in cellular metabolism. (lu.se)
  • During his postdoc, he carried out research to determine the role of Kruppel-like factor 2 (KLF2) during osteoblast (OB) differentiation of dental pulp-derived stem cells (DPSC). (nih.gov)
  • This systematic review aims to provide a panel of miRNAs that regulate the differentiation of hDP-MSCs including hDPSCs and SHEDs. (biomedcentral.com)
  • The findings of this systematic review support the potential application of the specific miRNAs to regulate the directed differentiation of hDP-MSCs in the field of regenerative therapies. (biomedcentral.com)
  • Our analysis demonstrates how early cell fate specification factors can regulate structural and physiological differentiation of neuronal cell types. (tcd.ie)
  • Recently, genetic analyses in Drosophila have revealed various genes that regulate the competitive behavior of cells. (fsu.edu)
  • To understand how the genome and epigenome regulates cellular function, development of high-throughput sequencing methods, known as next-generation sequencing, are beginning to unravel genome-wide correlations between the genome, epigenome and transcriptome within a large population of cells or tissues. (confex.com)
  • WRN critically regulates autophagy in various cellular stresses, including starvation, endoplasmic reticulum (ER) stress conditions to rescue the cell, and basal situations to restore proper cellular function. (nih.gov)
  • These results provide evidence that the involvement of mitophagy is crucial during OB differentiation, and KLF2 critically regulates it. (nih.gov)
  • The ultrastructural study of the cellular modifications (during the life cycle reversion of T. nutricula) showed the presence of both degenerative and apoptotic processes. (researchgate.net)
  • Basally extruded apoptotic cells were excluded from the analysis. (fsu.edu)
  • GO and KEGG analyses indicated that numerous DE lncRNAs might be involved in diverse biological processes related to phosphate, such as lipid metabolic processes, catalytic activity, cell membrane formation, signal transduction, and nitrogen fixation. (biomedcentral.com)
  • Nevertheless, a lack of knowledge relating to the mechanisms regulating their differentiation has limited their clinical application. (biomedcentral.com)
  • Although MSC-derived EVs (mEVs) are beneficial for acute lung injury and pulmonary fibrosis, mechanisms of mEV uptake by lung fibroblasts and their effects on myofibroblastic differentiation have not been established. (nih.gov)
  • The DECM seeded with human nasal chondrocytes (HPCH) were cultured in the glass reactor for 6 weeks and examined by histological and immunohistochemical staining, biochemical analyses and real time-PCR at 14, 28 and 42 days. (uni-augsburg.de)
  • Due to similar morphological features of PTPR with a number of other papillary-like tumors of the pineal region, immunohistochemical analysis is crucial to its distinction. (surgicalneurologyint.com)
  • Here, through the use of conditional knock-out mice, we demonstrate a requirement for ILK in oligodendrocyte differentiation and axonal myelination in vivo . (jneurosci.org)
  • Intracellular flow cytometry is a powerful technique for the identification of cell types and the analysis of signaling and functional responses within cell lines and heterogeneous cell samples. (bdbiosciences.com)
  • Murine Rab11a and Rab11b were found to localize in distinct cellular compartment, suggesting functional differentiation [9]. (researchsquare.com)
  • Additionally, some gene sequences overlapped with transposons, suggesting replication and functional differentiation of carotenoid-related genes during carrot evolution. (bvsalud.org)
  • From an engineering perspective, gene expression regulation can be viewed as the output of a network of complex chemical and physical processes, and understanding how these processes interact and integrate to govern cellular phenotypes has been a major focus of my graduate and postdoctoral research. (confex.com)
  • The purpose of this study was to develop a convenient in vitro model system for glioblastoma differentiation and to then characterize it using conventional techniques and flow cytometry. (northwestern.edu)
  • Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. (wikipedia.org)
  • However, metabolic composition does get altered quite dramatically where stem cells are characterized by abundant metabolites with highly unsaturated structures whose levels decrease upon differentiation. (wikipedia.org)
  • This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
  • We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
  • Therefore, in addition to epidermal Langerhans cells, other potential cellular origins for LCH include dermal langerin + dendritic cells, lymphoid tissue-resident langerin + dendritic cells, and monocytes that can be induced by local environmental stimuli to acquire a Langerhans cell phenotype. (medscape.com)
  • Autophagy is a cytoplasmic conserved catabolic phenomenon to maintain cellular homeostasis. (nih.gov)
  • Here we present high-resolution analyses of chromosomal organization in pluripotent and differentiated human and murine cell types determined using the Hi-C technique. (nih.gov)
  • In this review we will discuss how combinations and fusions of different -omic workflows on a single cell level can be used to examine cellular phenotypes, immune effector functions, and even dynamic changes, such as metabolomic state of different cells in a sample or even in a defined tissue location. (frontiersin.org)
  • Taking advantage of the developmental gradient in the bread wheat leaf, we provide a simultaneous quantitative analysis for the development of mesophyll cells and of chloroplasts as a cellular compartment. (biomedcentral.com)
  • Differentiation subsequently occurs in leaf primordia basipetally, resulting in a gradient of easily distinguishable cellular morphologies of distal differentiated cells towards the tip of the leaf, basal progenitor proliferating cells adjacent to the shoot apical meristem, and all possible intermediate stages in between. (biomedcentral.com)
  • The migration depth and the number of migrated cells were quantified by computational analysis. (uni-augsburg.de)
  • Furthermore, cultivation in the bioreactor enhanced differentiation of the cells during the first 14 days, but differentiation diminished in the course of further cultivation. (uni-augsburg.de)
  • Simultaneous measurements of glycolysis and oxidative phosphorylation in live cells revealed that OB differentiation decreased the oxygen consumption rate, which indicates reduced ATP production and mitochondrial respiration. (nih.gov)
  • Its effect and effectiveness appear to be highly connected with the differentiation status of the cancerous cells, as Ang II appears to play a crucial role in the early and late stages of malignant transformation. (spandidos-publications.com)
  • In summary, the three major areas of research in my lab are independent yet connected-proper differentiation of follicle cells is key to the establishment of oocyte polarity, and cell competition involves intercellular communication and regulation of cell proliferation. (fsu.edu)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • Specifically, a variety of other cellular populations have been identified that possess phenotypic characteristics similar to Langerhans cells, including expression of CD207 and Birbeck granules. (medscape.com)
  • Maintaining differentiation of chondrocytes and improving cellular migration by further optimizing culture conditions is an important prerequisite for future clinical application. (uni-augsburg.de)
  • Tasks: This project includes carrying out data integration, clustering and network analysis to define metabolic phenotypes in asthma. (lu.se)
  • Some differentiation occurs in response to antigen exposure. (wikipedia.org)
  • Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)
  • By applying classical OB differentiation methods and a phytoestrogen ferutinin stimulated OB differentiation procedure, Dr. Maity showed that the levels of KLF2 and autophagy-related molecules increase during osteoblast differentiation. (nih.gov)
  • However, each differentiation method has a different impact on cellular metabolic changes. (nih.gov)
  • Clinicopathological parameter correlation and in silico network analysis have also implicated. (hindawi.com)
  • In silico analyses indicated that IPF lung fibroblasts have increased expression of genes that are targets of mEV-enriched miRs. (nih.gov)
  • Intracellular flow cytometry also provides rich information concerning cellular function and signaling responses. (bdbiosciences.com)
  • In light of these findings, precisely how β1 integrin transduces ECM cues via signaling intermediates, and the mechanism by which this translates to cellular responses, need to be addressed. (jneurosci.org)
  • By contrast, cellular responses to cross-reactive epitopes may provide a substantial degree of protection against serologically distinct viruses ( 9 ). (cdc.gov)
  • CCK-8 assay, flow cytometric analysis, western blotting, orthotopic GBM model as well as transwell assay were performed to investigate the biological role of CASP5. (cancerindex.org)
  • Multiparameter flow cytometry is a useful technique for characterizing such differentiation. (northwestern.edu)
  • Flow cytometry analysis of forebrain-derived and developing CNS in rodents (Rietze et al. (lu.se)
  • Among the patients with survival data 27 patients had RO resections and these cases were considered in survival analysis. (biomedcentral.com)
  • In the univariate analysis, neither TGF-β1 nor p27 expression was related with patient survival. (biomedcentral.com)
  • Characterization of these factors is lacking in porcine conceptuses, therefore, the current study investigated mRNA expression of these factors in elongating conceptuses and primordial tissues as well as protein expression and cellular localization to better define their biological significance. (usda.gov)
  • This approach extends the analysis of complex systems from description to prediction, including control of complex dynamic behavior ranging from biological rhythms to catastrophic lethal arrhythmias. (mdpi.com)
  • The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)
  • OBJECTIVE: Induction of cellular differentiation continues to be an attractive therapeutic strategy for malignant glioma. (northwestern.edu)
  • Expression profiles analysis identifies the values of carcinogenesis and the prognostic prediction of three genes in adrenocortical carcinoma. (cancerindex.org)
  • In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. (wikipedia.org)
  • Cytogenic analysis and comparative genomic hybridization (CGH) studies have demonstrated that both WDLPS and DDLPS are characterized by supernumerary ring chromosomes or giant marker chromosomes and12q13-15 region amplifications, resulting in MDM2 and CDK4 overexpression. (sarcomahelp.org)
  • Genomic and transcriptomic analysis has furthered our understanding of many tumors. (vanderbilt.edu)
  • These megabase-long genomic structures, defined by prominent local chromatin interactions and separated by narrow boundary regions, likely function to restrict regulatory interactions between cis-acting elements and limit the spreading of heterochromatin during cellular differentiation. (nih.gov)
  • Similarly, in specific cases, such as cancers, viral infections and in certain cell-types during normal development, mutations and other structural variations within the genome also influences cellular functions. (confex.com)
  • Subsequently, analysis pipelines have become more time consuming and complicated, usually requiring highly extensive prevalidation steps. (ghenry.info)
  • Single cell proteome approaches already have changed how we explore cellular mechanism in disease and during therapy. (frontiersin.org)
  • Moreover, lncRNA-mRNA-miRNA and lncRNA-mRNA networks were constructed, and the results identified several promising lncRNAs that might be highly valuable for further analysis of the mechanism underlying the response of soybean to LP stress. (biomedcentral.com)
  • Differentiation happens multiple times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. (wikipedia.org)
  • Western blotting and/or immunohistochemistry were utilized to examine protein expression and cellular localization. (usda.gov)
  • However, the expression analysis of rab11 is so far lacking. (researchsquare.com)
  • Experimental studies assessing the promotive/suppressive effect of miRNAs on the differentiation of hDP-MSCs and studies evaluating changes to the expression of miRNAs during the differentiation of hDP-MSCs were included. (biomedcentral.com)
  • p27 expression is reduced in pancreatic adenocarcinomas and decreased protein levels of p27 may play a role in the differentiation of pancreatic cancer. (biomedcentral.com)
  • Northern blot and immunoblot analyses of c-myc expression were performed. (northwestern.edu)
  • RESULTS: Butyrate treatment produced a dose-dependent inhibition of cellular proliferation and changes in morphology, GFAP staining, and c-myc expression consistent with a differentiation response. (northwestern.edu)
  • The second phase is marked by the development of photosynthetic chloroplasts which occupy the available cellular space. (biomedcentral.com)
  • Together, our work demonstrates ILK as necessary for normal oligodendrocyte development, reinforces its role as a bridge between the actin cytoskeleton and cell membrane, and highlights the overarching compensatory capacity of oligodendrocytes in response to cellular milieu. (jneurosci.org)
  • This paper represents a valuable single-cell level analysis of tendon enthesis development. (elifesciences.org)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • Using cesarean-delivered preterm pigs as a model of preterm infants, we investi-gated the effects of supplemental IGF-1 on motor function and on regional and cellular brain development. (lu.se)
  • After a day of rest, lambs were harvested, and pre- rigor Longissimus dorsi-muscles were obtained for transcriptomic analysis. (unl.edu)
  • Cellular differentiation is the process in which a stem cell changes from one type to a differentiated one. (wikipedia.org)
  • genetics of a complex differentiation process. (lu.se)
  • ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
  • More striking, the pancreas in these mice appeared relatively normal, with well-demarcated islets, suggesting that Pax4 misexpression was inducing an orderly and spatially correct program of cellular conversion that probably recapitulated the normal cellular differentiation process. (medscape.com)
  • With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. (wikipedia.org)
  • Differentiation involves morphogenesis but also cell-appropriate organelle biogenesis programs. (biomedcentral.com)