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  • amino acid seq
  • The deduced amino acid sequence of the open reading frame of the human cDNA is 99% homologous to mouse Otp and demonstrates a high degree of conservation when compared to sea urchin and Drosophila. (nih.gov)
  • A single putative OTP gene product was found in 17-week human fetal brain tissue by Western blot analysis using a novel polyclonal antibody raised against a conserved 13-amino-acid sequence at the C-terminus of the OTP protein. (nih.gov)
  • functional
  • The striking functional equivalence of Hox3 paralogues was most dramatically demonstrated by swapping the Hoxa3 and Hoxd3 protein coding sequences, leaving the regulatory regions intact ( 15 ). (pnas.org)
  • cDNA
  • Human cDNA and genomic libraries were screened with probes derived from mouse Otp sequences to find the human homologue, OTP. (nih.gov)
  • In this study, a longan embryogenic callus (EC) cDNA library was sequenced using an Illumina HiSeq 2000 system. (biomedcentral.com)
  • reveals
  • High-throughput resequencing of 1 Mb of rat chromosome 14 from dmbo/dmbo rats, encompassing the Hmx1 locus, reveals numerous divergences from the rat genomic reference sequence, but no coding changes in Hmx1 . (biologists.org)
  • Further sequence analysis of this region reveals a 5777-bp deletion located ∼80 kb downstream in dmbo/dmbo rats that is not apparent in 137 other rat strains. (biologists.org)
  • Analysis of 2,086 conserved sequence blocks (CSBs), identified from 135 characterized enhancers, reveals most CSBs consist of shorter overlapping/adjacent elements that are either enhancer type-specific or common to enhancers with divergent regulatory behaviors. (biomedcentral.com)
  • Close inspection of enhancer MCSs reveals that these sequences are made up of smaller blocks of conserved sequences, designated here as 'conserved sequence blocks' (CSBs). (biomedcentral.com)
  • EvoPrint analysis of enhancer CSBs reveals that many have remained unchanged for over 160 million years (My) of collective divergence [ 9 ] (and see below). (biomedcentral.com)
  • significantly
  • Importantly, the sequence context of a binding site significantly influences binding energetics, and utilizing the full contextual information permits greater accuracy in annotating regulatory elements within a given genome. (pnas.org)
  • mutations
  • Mouse dmbo , mouse mpe and the known human Hmx1 allele causing OAS are all coding mutations that affect the Hmx1 DNA-binding homeodomain, and thus are predicted to result in loss of function. (biologists.org)
  • partial
  • Based on the partial homeobox sequences we cannot unambiguously distinguish between Scr and Antp homeobox fragments in Diplura, Archaeognatha, Ephemeroptera, Odonata, Plecoptera and Dermaptera. (nih.gov)
  • vertebrates
  • Comparison of Hoxb-1 regulatory regions from different vertebrates identified three related sequence motifs critical for rhombomere 4 (r4) expression in the hindbrain. (naver.com)
  • transcriptome
  • We applied a high throughput RNA sequencing (RNA-seq) strategy using Illumina GAIIx to characterize the entire retinal transcriptome from nondiabetic and from streptozotocin-treated mice 32 weeks after induction of diabetes. (molvis.org)
  • Analysis of the global transcriptome of longan (Dimocarpus longan Lour. (biomedcentral.com)
  • temporal sequence
  • These results illustrate that turning Wnt signaling off and on in the correct temporal sequence is essential for organ formation, a finding that might directly impact efforts to differentiate liver and pancreas tissue from stem cells. (biologists.org)
  • bind
  • Pax-6 5a, however, is able to bind to a recently described sequence called 5aCON ( 6 ). (pnas.org)
  • motifs
  • However, elucidating TF binding site (TFBS) motifs in higher eukaryotes has been challenging, even when employing cross-species sequence conservation. (biomedcentral.com)
  • insects
  • A Neighbour Joining analysis of only the six potentially unambiguous new homeobox sequences (lab, pb, Dfd, Ubx, Abd-B) groups all new fragments into the expected clades of homologs from other insects and thus confirms the results of NCBI Blast (Fig. 2), except for abd-A which appears paraphyletic. (nih.gov)