Loading...
  • cytotoxicity
  • Inhibition of MDA-MB-231 cell autophagy, by reduction of ATG7 or 3-methyladenine treatment, did not block this 8-NH 2 -Ado-mediated cytotoxicity. (aacrjournals.org)
  • Gemcitabine activates DNA damage pathways that signal to the tumor suppressor p53 ( 1 ), and a functional p53 pathway improves gemcitabine cytotoxicity ( 2 ). (aacrjournals.org)
  • The nucleoside analogues 8-amino-adenosine and 8-chloro-adenosine have been investigated in the context of B-lineage lymphoid malignancies by our laboratories because of the selective cytotoxicity they exhibit toward multiple myeloma (MM), chronic lymphocytic leukemia (CLL), and mantle cell lymphoma (MCL) cell lines and primary cells. (biodiversityhotspot.org)
  • Taken collectively, these results reveal that G6PD activity antagonizes the cytotoxicity of 8-substituted adenosine analogues and shows that administration of the agents to individuals with B-cell malignancies exhibiting regular levels of manifestation may be especially efficacious. (biodiversityhotspot.org)
  • triphosphate
  • A mixture containing 200 nM 5′- 32 P-radiolabeled RNA primer, 1 ng bovine PAP/μL reaction, 250 μM analog triphosphate, 10 mM Tris-HCl (pH 8.5), 0.5 mM MnCl 2 , 1% polyvinyl alcohol was incubated at 30 °C for 30 mins. (nih.gov)
  • Molecular
  • To explain these experimental observations and probe the possible structural mechanisms, molecular modeling was employed to examine the interactions between PAP and various ATP analogues. (nih.gov)
  • cells
  • therefore, we asked if 8-NH 2 -Ado could kill breast cancer cells without activating the p53-pathway. (aacrjournals.org)
  • Regardless of the breast cancer subtype tested or the p53 status of the cells, 8-NH 2 -Ado was more cytotoxic than either gemcitabine or etoposide. (aacrjournals.org)
  • 8-NH 2 -Ado induced apoptotic death of MCF-7 cells and apoptosis was not inhibited by knockdown of functional p53. (aacrjournals.org)
  • Interestingly, 8-NH 2 -Ado caused the MDA-MB-231 cells to detach from the plate with only limited evidence of apoptotic cell death markers and the cell death was not inhibited by Z-VAD. (aacrjournals.org)
  • Importantly 8-NH 2 -Ado was highly cytotoxic to triple-negative breast cancer cells and worked through a pathway that did not require wild-type p53 for cytoxicity. (aacrjournals.org)
  • A unique RNA-directed nucleoside analog is cytotoxic to breast cancer cells and depletes cyclin E levels. (nih.gov)
  • Intro The book nucleoside analogues 8-amino-adenosine (8-NH2-Ado) and 8-chloro-adenosine (8-Cl-Ado) possess undergone extensive preclinical advancement for tumor treatment by our laboratories because of the exclusive RNA-directed results they elicit in tumor cells. (biodiversityhotspot.org)
  • This idea is supported with the observation that cells missing TAK 165 adenosine kinase appearance are totally resistant to 8-NH2-Ado or 8-Cl-Ado treatment , [2]. (biodiversityhotspot.org)
  • Deposition of high micromolar to low millimolar degrees of 8-Cl-ATP in peripheral bloodstream mononuclear cells (PBMC) pursuing administration of 8-Cl-Ado to mice and rats provides proof helping the auspicious scientific leads of 8-substituted adenosine analogues to take care of lymphoid Rabbit Polyclonal to GABA-B Receptor neoplasms. (biodiversityhotspot.org)
  • 8-Chloro-adenosine (8-Cl-Ado), a nucleoside analog, can inhibit proliferation in a variety of human tumor cells. (chemweb.com)
  • Flow cytometric analysis of nuclear DNA distribution Cells (2×106) were collected, rinsed and fixed overnight with 70% cold ethanol. (biobeds.info)
  • Patients
  • Gemcitabine is a nucleoside analog that is approved by the U.S. Food and Drug Administration to be used in combination with paclitaxel for the first-line treatment of patients with metastatic breast cancer after they have received anthracycline chemotherapy. (aacrjournals.org)
  • Poly
  • The nucleotide substrate specificity of bovine poly(A) polymerase (PAP) towards C8-modified ATP analogues was examined using primer extension assays. (nih.gov)
  • various
  • The halogen in position 2 of the adenine nucleobase can be used for various sustitutions including spacers and labels. (biolog.de)
  • Agents
  • 2. The method of claim 1, wherein the composition provides sustained release of the one or more active agents into or in the vicinity of one or more sinonasal cavities for a period of at least 5 days. (sumobrain.com)
  • mice
  • Male C57BL/6 (Taconic, Germantown, NY) body weight-matched A 3 R KO mice 2 (crossed back to Taconic C57BL/6 mice at least 16 times), 8 to 12 weeks old, were used for TAC or control. (ahajournals.org)
  • administration
  • 5. The method of claim 2, wherein said effective concentration is achieved by intravenous administration to a mammal. (google.es)
  • treatment
  • Therefore, 8-NH 2 -Ado should be considered for the treatment of triple-negative breast cancers that are chemotherapy resistant. (aacrjournals.org)
  • activation
  • In addition, both acid production and radioligand binding of adenosine analogs to isolated cell membranes were potently blocked by selective A2BR antagonists, whereas ligands for A 1 , A 2A , and A 3 adenosine receptors failed to abolish activation. (physiology.org)
  • The primary stimulatory processes are considered to be of histaminergic nature via G s -coupled H 2 receptor activation and of cholinergic nature via activation of G q -coupled muscarinic receptors or receptors for gastrin ( 69 ), but other actors are also on the stage. (physiology.org)
  • found
  • The released DNA was structurally intact (but found primarily in the open conformation after 2 d of release) and transfection competent. (imbc-med.ru)