Leucine aminopeptidaseAlanineMethionine AminopeptidaseEnzymeInsulin-regulated aminopeptidaseIRAPLeucylDipeptidylEndoplasmic reticulumInhibitorsERAP1ProteinInhibitorPorcineFingerprintSubstrateMembrane-boundAnpepProteinsAmino acidsGeneBacterialActivityReceptorHumanCOMPLEXHighChemistryPresentDomainEssential
Leucine aminopeptidase3
- A heat-stable aminopeptidase with similar biochemical properties to eukaryotic leucine aminopeptidase was purified to homogeneity from Escherichia coli K-12 by Vogt and named aminopeptidase I. The bacterial leucyi aminopeptidases range from 491 to 524 amino acids in length. (gla.ac.uk)
- During human pregnancy, a circulating form of insulin-regulated aminopeptidase (IRAP EC 3.4.11.3), often termed oxytocinase or placental leucine aminopeptidase (PLAP), is present in plasma. (monash.edu)
- Upon exposure to LAP (leucine aminopeptidase: LAP), the amide bond in CPT-p-Leu will be cleaved, followed by an intramolecular 1,6-elimination, which triggers the active anticancer drug (CPT) release and recovers the fluorescence of CPT. (bath.ac.uk)
Alanine4
- In particular, we evaluated 6 different substrates to reproduce broad-specificity aminopeptidase N, broad-specificity aminopeptidase C, glutamyl aminopeptidase A, peptidase with high specificity for leucine and alanine, proline iminopeptidase, and X-prolyl dipeptidyl aminopeptidase activities releasing different N-terminal amino acids. (unipr.it)
- Increasing salt concentration slowed down broad-specificity aminopeptidase C, glutamyl aminopeptidase A, proline iminopeptidase, and peptidase with high specificity for leucine and alanine. (unipr.it)
- The kidney effects of halothane, enflurane and isoflurane were evaluated by using the ratio of urinary excretion of alanine aminopeptidase (AAP) to urine creatinine. (hacettepe.edu.tr)
- The authors conclude that a significant risk of overexposure to cadmium dust did exist at this facility and that subclinical effects such as significant increases in mean levels of urinary tubular enzymes alanine-aminopeptidase and N-acetyl- glucosaminidase are apparent in cadmium exposed workers with urine cadmium levels above 10microg/g of creatinine compared to those below this level. (cdc.gov)
Methionine Aminopeptidase4
- Methionine aminopeptidase (MetAP) is a ubiquitous enzyme required for cell survival and an attractive target for antibacterial and anticancer drug development. (biomedcentral.com)
- Herein, we report the application of a robust nonlinear regression approach in determination of the number of the catalytically relevant metal ion required by the metalloprotease methionine aminopeptidase (MetAP). (biomedcentral.com)
- Methionine aminopeptidase 1 (MtMET-AP1) is a hydrolase that mediates the necessary post-translational N-terminal methionine excision (NME) of peptides during protein synthesis, which is necessary for bacterial proliferation and is a potential target for the treatment of tuberculosis. (bvsalud.org)
- The proteins LTA4H (leukotriene A-4 hydrolase), METAP1 (methionine aminopeptidase 1), BID (BH3-interacting domain death agonist), SMAD1 (mothers against decapentaplegic homologue), PRKRA (interferon-inducible double-stranded RNA-dependent protein kinase activator A), YES1 (tyrosine-protein kinase Yes) and EGFL7 (epidermal growth factor-like protein 7) were significantly upregulated in EGFRI-stimulated peripheral blood mononuclear cell cultures, and validated in the lesions. (bvsalud.org)
Enzyme8
- One important aminopeptidase is a zinc-dependent enzyme produced and secreted by glands of the small intestine. (wikipedia.org)
- This review discusses novel insights (including the data of recent clinical trials) with regard to interference with the renin-angiotensin system, focusing on the enzymes aminopeptidase A and angiotensin-converting enzyme 2 (ACE2) in the brain, as well as the substrate of renin- angiotensinogen-in the liver. (nih.gov)
- First, this investigation confirmed the gene was transcribed and the transcript encoded a functional enzyme with M1 aminopeptidase characteristics. (mtsu.edu)
- The modeling approach shows that to highlight the individual and interactive effects of chemical-physical variables on enzyme activities, it is helpful to determine the true potential of an aminopeptidase in cheese. (unipr.it)
- Its carboxy-terminal domain located inside endosomes is responsible for the aminopeptidase activity of the enzyme, while its amino-terminal domain located in the cytosol functions as an endosomal trafficking adaptor. (cri1149.fr)
- This protein is an aminopeptidase, which is an enzyme that cuts (cleaves) other proteins into smaller fragments called peptides. (medlineplus.gov)
- Disruption of the lysosomes by nonionic detergents resulted in the expression of latent activity of the enzyme dipeptidyl aminopeptidase-I(DAP-I). Duchenne lysosomes showed less structure-linked latency than those from normal controls, and sedimentation studies demonstrated that the difference was not caused by increased leakage of the enzyme from lysosomes. (utmb.edu)
- Leukotriene A 4 hydrolase (LTA 4 H) is a bi-functional enzyme that promotes a pro-inflammatory epoxy hydrolase (EH) pathway and an anti-inflammatory aminopeptidase (AP) pathway. (gmu.edu)
Insulin-regulated aminopeptidase1
- Insulin regulated aminopeptidase (IRAP) is a type II transmembrane protein with broad tissue distribution initially identified as a major component of Glut4 storage vesicles (GSV) in adipocytes. (cri1149.fr)
IRAP1
- The insulin-responsive aminopeptidase (IRAP/VP165/gp160) was identified originally in GLUT4-containing vesicles and shown to translocate in response to insulin, much like the glucose transporter 4 (GLUT4). (johnshopkins.edu)
Leucyl5
- Colloms, S. D. (2004) Leucyl aminopeptidase PepA. (gla.ac.uk)
- This chapter examines the structural chemistry and the biological aspects of Leucyl aminopeptidase PepA. (gla.ac.uk)
- Leucyl Aminopeptidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
- This graph shows the total number of publications written about "Leucyl Aminopeptidase" by people in this website by year, and whether "Leucyl Aminopeptidase" was a major or minor topic of these publications. (wakehealth.edu)
- Below are the most recent publications written about "Leucyl Aminopeptidase" by people in Profiles. (wakehealth.edu)
Dipeptidyl1
- Interestingly, this variable did not affect broad-specificity aminopeptidase N and positively affected X-prolyl dipeptidyl aminopeptidase. (unipr.it)
Endoplasmic reticulum7
- cDNA sequences are available for several aminopeptidases and a crystal structure of the open state of human endoplasmic reticulum Aminopeptidase 1 ERAP1 is presented here. (wikipedia.org)
- The ERAP1 gene provides instructions for making a protein called endoplasmic reticulum aminopeptidase 1. (medlineplus.gov)
- Endoplasmic reticulum aminopeptidase 1 has two major functions, both of which are important for normal immune system function. (medlineplus.gov)
- Second, endoplasmic reticulum aminopeptidase 1 cleaves many types of proteins into small peptides that can be recognized by the immune system. (medlineplus.gov)
- Each of the ERAP1 gene variants changes a single protein building block (amino acid) in endoplasmic reticulum aminopeptidase 1. (medlineplus.gov)
- Researchers believe that changes in the structure of endoplasmic reticulum aminopeptidase 1 could alter either of its two major functions. (medlineplus.gov)
- Haroon N, Tsui FW, Uchanska-Ziegler B, Ziegler A, Inman RD. Endoplasmic reticulum aminopeptidase 1 (ERAP1) exhibits functionally significant interaction with HLA-B27 and relates to subtype specificity in ankylosing spondylitis. (medlineplus.gov)
Inhibitors1
- Specific puromycin-sensitive aminopeptidase (PSA) inhibitors with a 3-(halogenated phenyl)-2,4(1H,3H)-quinazolinedione skeleton were prepared and their structure-activity relationships were investigated. (elsevierpure.com)
ERAP11
- The ER aminopeptidase, ERAP1, trims precursors to lengths of MHC class I peptides by a 'molecular ruler' mechanism. (medlineplus.gov)
Protein3
- The protein encoded by this gene belongs to the family of X-pro-aminopeptidases that utilize a metal cofactor, and remove the N-terminal amino acid from peptides with a proline residue in the penultimate position. (nih.gov)
- As for several other alphacoronaviruses including the porcine transmissible gastroenteritis virus (TGEV) and the human coronavirus 229E (HCoV-229E), the aminopeptidase N (APN) protein was reported to be a functional receptor for PEDV. (uu.nl)
- By comparison of systemic analysis results for Acanthamoeba isolates, we identified a novel secreted protein of Acanthamoeba, an M28 aminopeptidase (M28AP), which targets of the human innate immune defense. (ncku.edu.tw)
Inhibitor2
- The aminopeptidase inhibitor amastatin was 40-fold more potent as an inhibitor of AmM activity (IC50 = 0.2 microM) than of AmA activity (IC50 = 8 microM). (aspetjournals.org)
- The structural results are compared with a recent structure of AAP with an hydroxamate inhibitor in order to draw general functional conclusions which are relevant for this family of low molecular-weight aminopeptidases. (huji.ac.il)
Porcine1
- Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. (k-state.edu)
Fingerprint1
- Dive into the research topics of 'Mammary gland development is delayed in mice deficient for aminopeptidase N'. Together they form a unique fingerprint. (elsevierpure.com)
Substrate3
- For methionine aminopeptidases (MetAPs), the exact location of this nucleophile, as well as of the substrate, with respect to the active site metal ion is unknown. (bepress.com)
- PepA is one of three broad-range aminopeptidases, with overlapping substrate specificity in E. coli and S. typhimurium. (gla.ac.uk)
- In addition it is demonstrated by fluorescence microscopy that viable, activated T-cells effectively cleave the fluorogenic aminopeptidase substrate bis-glycyl-rhodamine 110 and that the corresponding aminopeptidase activity is associated with the cell surface. (silverchair.com)
Membrane-bound1
- CD13/aminopeptidase N (APN) is a membrane-bound, zinc-dependent metalloproteinase that plays a key role in tumor invasion and angiogenesis. (elsevierpure.com)
Anpep1
- We have analysed the role of the cell surface protease aminopeptidase N (Anpep, APN, CD13) during these processes using Anpep deficient and Anpep over-expressing mice. (elsevierpure.com)
Proteins1
- Aminopeptidases are enzymes that catalyze the cleavage of amino acids from the amino terminus (N-terminus) of proteins or peptides (exopeptidases). (wikipedia.org)
Amino acids1
- The aim of this work was to investigate in which phases of ripening of Parmigiano Reggiano cheese lactic acid bacteria aminopeptidases present in cheese extract could be involved in release of free amino acids and to better understand the behavior of these enzymes in physical-chemical conditions that are far from their optimum. (unipr.it)
Gene3
- The M1 aminopeptidase family was then studied in depth to identify conservations within the gene structures that may indicate potential functional role(s) of RNPEPL1. (mtsu.edu)
- In conclusion, RNPEPL1 is a true gene that transcribes a functional M1 aminopeptidase. (mtsu.edu)
- C . difficile was presumptively identified on the basis of Gram stain and detection of L-proline aminopeptidase activity (Pro Disc, Remel, Lenexa, KS, USA) and confirmed by identification of the triose phosphate isomerase gene ( 9 ). (cdc.gov)
Bacterial1
- Within the bacterial leucyi aminopeptidases, there is also more sequence similarity between catalytic C-terminal domains than between N-terminal domains. (gla.ac.uk)
Activity2
- Structure of the human aminopeptidase XPNPEP3 and comparison of its in vitro activity with Icp55 orthologs: Insights into diverse cellular processes. (nih.gov)
- Role of aminopeptidase activity in the regulation of the pressor activity of circulating angiotensins. (aspetjournals.org)
Receptor1
- The requirement for aminopeptidase N (APN) as a receptor for TGEV, but not for PEDV, is well established. (illinois.edu)
Human3
- Aminopeptidase P isozyme expression in human tissues and peripheral blood mononuclear cell fractions. (nih.gov)
- Arginyl aminopeptidase-like 1 (RNPEPL1) was first reported as a hypothetical reading frame during various genomic studies to complete the human genome. (mtsu.edu)
- This study followed the twelve human members, eleven mouse members, and eleven rat members of the M1 aminopeptidase family through multiple alignments, phylogenetic trees, synteny studies, and exon structure analyses. (mtsu.edu)
COMPLEX1
- Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2. (nih.gov)
High2
- Some aminopeptidases are monomeric, and others are assemblies of relatively high mass (50 kDa) subunits. (wikipedia.org)
- The motivation for the detailed structural analysis of SGAP originated from a strong mechanistic interest in the family of double-zinc aminopeptidases, combined with the high potential applicability of these enzymes. (huji.ac.il)
Chemistry1
- Garza, LA & Birnbaum, MJ 2000, ' Insulin-responsive aminopeptidase trafficking in 3T3-L1 adipocytes ', Journal of Biological Chemistry , vol. 275, no. 4, pp. 2560-2567. (johnshopkins.edu)
Present2
- The aminopeptidases tested in this work were present in cell-free Parmigiano Reggiano cheese extract after a 17-mo ripening and were active when tested in model system. (unipr.it)
- SGAP is an aminopeptidase present in the extracellular fluid of Streptomyces griseus cultures. (huji.ac.il)
Domain1
- Alignment of E. coli PepA with bovine lens leucyi aminopeptidase reveals 44% sequence identity in the catalytic C-terminal domain, but only 18% identity in the N-terminal domain. (gla.ac.uk)
Essential2
- Aminopeptidases are used in essential cellular functions. (wikipedia.org)
- Trageting the aminopeptidases PfAM1 & PfAM17 essential for the parasite at various stages of its development, offers new opportunities in antimalarial therapeutics. (anr.fr)