• The information within the KdpD protein that confers SRP interaction was found in the amino-terminal cytoplasmic domain of KdpD, particularly at residues 22-48. (nature.com)
  • Within this sequence a Walker A motif is present at residues 30-38. (nature.com)
  • When the KdpD peptides (residues 22-48) were fused to sfGFP the targeting to the membrane was observed by fluorescence microscopy. (nature.com)
  • We found that the amino acid residues R22, K24 and K26 are important for SRP interaction, whereas the residues G30, G34 and G36, essential for a functional Walker A motif, can be replaced with alanines without affecting the affinity to SRP-FtsY and membrane targeting. (nature.com)
  • Furthermore, it is suggested that the binding of SRP to the signal sequence is promoted by the presence of basic amino acid residues through electrostatic interactions 18 . (nature.com)
  • Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
  • The intracellular loops of GPCRs are crucial for G-protein interactions, and activation of CXCR1 involves both amino-terminal residues and extracellular loops. (rcsb.org)
  • Our previous nuclear magnetic resonance studies indicate that IL-8 binding to the N-terminal residues is mediated by the membrane, underscoring the importance of the phospholipid bilayer for physiological activity. (rcsb.org)
  • The N-proximal portion is devoid of positively charged residues as well as the amino acids glycine and proline. (silverchair.com)
  • KdpD is a four-spanning membrane protein that has two large cytoplasmic domains at the amino- and at the carboxyterminus, respectively. (nature.com)
  • The deduced amino acid sequence predicted a 353 amino acid polypeptide with a single membrane spanning segment and a 34 amino acid hydrophilic COOH-terminal cytoplasmic domain. (rupress.org)
  • Analysis of the cDNA and deduced amino acid sequences revealed a high degree of identity with the transmembrane and cytoplasmic domains of previously described proteoglycans but a unique extracellular domain sequence. (rupress.org)
  • The mouse EphA5 cDNA encodes an 877 amino acid (aa) precursor that includes a 26 aa signal sequence, a 386 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 444 aa cytoplasmic domain (5). (rndsystems.com)
  • The MUC16CTD anchors the protein at the cell surface and consists of a 229 amino acid extracellular region containing a potential proteolytic cleavage site, a 23 residue transmembrane domain, and a 31 amino acid cytoplasmic tail. (biomedcentral.com)
  • Ankyrin repeats are about 33 amino acids long and occur in at least four consecutive copies. (embl.de)
  • In contrast, isoform 3 (UniProtKB: Q9H9R9-3) carries a 100 amino acids long N-terminal sequence extension. (springer.com)
  • The primary sequence of PrP is 253 amino acids long before posttranslational modification. (biolegend.com)
  • We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)
  • Their amino acid sequences suggest that they are mainly cytosolic or nuclear proteins partly associating with membranes (Talbot et al. (springer.com)
  • We propose that Toc64 functions early in preprotein translocation, maybe as a docking protein for cytosolic cofactors of the protein import into chloroplasts. (silverchair.com)
  • The sequence conservation of the putative dysbindin domain across all selected species is notable (Fig. 1 shaded region). (springer.com)
  • Eleven candidate effector genes were identified based on features common to characterized fungal effectors, i.e. they encode small, soluble (lack of transmembrane domain), cysteine-rich proteins with a putative SP. (biomedcentral.com)
  • In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
  • In general, the signal for insertion into the inner bacterial membrane is located in the first hydrophobic transmembrane domain and insertion is catalysed by the Sec translocase and/or YidC insertase. (nature.com)
  • The TIM23 complex is a hub for translocation of preproteins into or across the mitochondrial inner membrane. (frontiersin.org)
  • The biogenesis of most proteins in the mitochondrial matrix and the inner membrane relies on the function of the TIM23 complex. (frontiersin.org)
  • The gating of this protein-selective pore has been extensively studied in patch clamp experiments on isolated mitochondrial membranes. (frontiersin.org)
  • Progress on sequencing and alignment analysis of higher plant mitochondrial genomes. (chinacrops.org)
  • 4]Xu G W, Cui Y X, Schertz K F, Hart G E. Isolation of mitochondrial DNA sequences that distinguish male sterility inducing cytoplasms in Sorghum bicolor L. Moench. (chinacrops.org)
  • Mitochondrial and chloroplast proteins are therefore synthesized in the cytosol as preproteins with an NH 2 -terminal targeting sequence, which is both necessary and sufficient for directing the preproteins to the correct organelle. (silverchair.com)
  • Mechanisms by which E. chaffeensis establishes intracellular infection, and avoids host defenses are not well understood, but involve functionally relevant host-pathogen interactions associated with tandem and ankyrin repeat effector proteins. (frontiersin.org)
  • Despite its small genome and limited number of effector proteins, Ehrlichia efficiently establishes an intracellular infection and avoids immune defenses in vertebrate and invertebrate hosts through complex molecular and cellular reprogramming strategies. (frontiersin.org)
  • Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
  • Features important for intracellular G-protein activation and signal transduction are revealed. (rcsb.org)
  • Multiple EphA5 splice variants exist and differ in their extracellular and intracellular sequences (5). (rndsystems.com)
  • Deletion of Mgr2p has been found to delay protein translocation into the matrix and accumulation in the inner membrane. (frontiersin.org)
  • The core of the TIM23 complex contains a pore that is essential for protein insertion into or translocation across the inner membrane. (frontiersin.org)
  • GTPase domain of the 54-kD subunit of the mammalian signal recognition particle is required for protein translocation but not for signal sequence binding. (escholarship.org)
  • In contrast, when SRP(-54G) was assayed for its ability to promote the translocation of preprolactin across microsomal membranes, it was completely inactive, apparently because it was unable to interact normally with the SRP receptor. (escholarship.org)
  • Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)
  • This is thought to make globular proteins stable and soluble in the watery environment of the cell. (anl.gov)
  • Sequences of ARF-GAP domains show no recognizable similarity to those of other GAPs, and contain a characteristic Cys-X(2)-Cys-X(16-17)-Cys-X(2)-Cys motif. (embl.de)
  • We performed a Basic Local Alignment Search Tool (BLAST) analysis to identify regions of local similarity between the human DBNDD1 and protein sequences from other species (Fig. 1 ). (springer.com)
  • Prion (PrP) is highly conserved through mammals and comparison between primates ranges from 92.9-99.6% similarity in amino acid sequence. (biolegend.com)
  • Caliciviruses are similar to picornaviruses in the pres- image reconstruction of recombinant Norwalk virus-like particles ence of VPg and in sequence similarity of their RNA-directed (left). (cdc.gov)
  • Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
  • Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. (shengsci.com)
  • This is the first report of any bacterial species possessing two genes for homologs of this major outer membrane protein. (shengsci.com)
  • During its biogenesis KdpD binds to the signal recognition particle (SRP) of Escherichia coli that consists of a 48-kDa protein Ffh and a 4.5S RNA. (nature.com)
  • Based on the Escherichia coli OmpA model, an eight-stranded amphipathic beta-barrel model for the membrane assembly of the N-terminal half of OmpAI and OmpAII was predicted. (shengsci.com)
  • An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. (shengsci.com)
  • PAPbeta, a protein that binds to and is phosphorylated by the non-receptor tyrosine kinase PYK2, contains several modular signaling domains including a pleckstrin homology domain, an SH3 domain, ankyrin repeats and an ARF-GAP domain. (embl.de)
  • Our recent studies suggested that the ret proto-oncogene protein is a cell surface receptor with a tyrosine kinase domain. (drugbank.com)
  • Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. (rndsystems.com)
  • The protein kinase is found in the cytosol of pea mesophyll cells or in wheat germ extract. (silverchair.com)
  • This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
  • In contrast to the signal sequences of exported proteins, the bacterial SRP signal sequences are more hydrophobic and are mostly "uncleaved signal sequences" present in membrane proteins that remain in the final protein-chain as transmembrane anchor sequences. (nature.com)
  • SRP at the ribosomal exit tunnel scans a nascent chain for bearing a hydrophobic SRP signal sequence 7 . (nature.com)
  • Current hypotheses on protein folding suggest that folding is initiated by formation of secondary structures followed by association of the hydrophobic regions of the emerging structure to orient them toward the interior of the protein in a process termed hydrophobic collapse. (anl.gov)
  • However, previous work by this team of researchers from the University of Notre Dame and The University of Chicago has shown that, contrary to popular belief, intrinsically disordered proteins, which sample a variety of conformational forms the way the autotransporter proteins do, can remain quite extended in water even if they have high ratios of hydrophobic to charged amino acids. (anl.gov)
  • This observation has led them to try and understand how proteins with high hydrophobic ratios can maintain these extended conformations. (anl.gov)
  • They made different versions of the pertactin domain by swapping amino acids to change the clustering of hydrophobic amino acids but keep the overall sequence and hydrophobicity ratio the same. (anl.gov)
  • They found that swapping just 6 of the 334 amino acids (2%) in the N-terminal domain of pertactin to increase hydrophobic clustering was enough to induce significant contraction of the structure. (anl.gov)
  • This finding suggests that spacing of hydrophobic amino acids must be under selective pressure in bacterial proteins to facilitate their virulence functions. (anl.gov)
  • This hypothesis was borne out by analysis of other autotransporter sequences that also appear to have patterning based on spacing of hydrophobic clusters. (anl.gov)
  • The central region is rich in the hydroxylated amino acids serine and threonine and overall is positively charged. (silverchair.com)
  • SRP is universally conserved in its core region that consists of a ribonucleoprotein particle, the SRP RNA (4.5S RNA in E. coli ) and the protein component SRP54 (Ffh in E. coli for "fifty-four-homolog") that binds to the conserved RNA domain IV 4 . (nature.com)
  • Interestingly, the in-silico analysis revealed that HomA/B consists of 8 discontinuous N and C terminal β-strands forming a small β-barrel, along with a large surface-exposed globular domain. (nature.com)
  • The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. (biolegend.com)
  • SRP54 consists of two separable domains, a 33-kD amino-terminal domain that contains a GTP-binding site (SRP54G) and a 22-kD carboxy-terminal domain (SRP54M) containing binding sites for both the signal sequence and SRP RNA. (escholarship.org)
  • Research has shown that the entire system consists of a protein complex that forms a large chamber that is anchored into the membrane. (diamond.ac.uk)
  • It shows a stack of six IkappaBalpha ankyrin repeats facing the C-terminal domains of the NF-kappaB Rel homology regions. (embl.de)
  • The encoded proteins (Mr = 33,564 and 32,536 for mature OmpAI and OmpAII, respectively) had only 64% identity with each other and otherwise had the highest level of homology to OmpA proteins from the members of the family Enterobacteriaceae. (shengsci.com)
  • 2009 ). The designated dysbindin paralogs show very limited sequence homology which raised the question whether DBNDD1 and DBNDD2 are dysbindin-like proteins or proteins that share a less conserved domain with DTNBP1 in the context of otherwise unrelated sequences (Ghiani and Dell'Angelica 2011 ). (springer.com)
  • Our chemical-shift based secondary structure analysis reveals the human DBNDD1 as an intrinsically disordered protein. (springer.com)
  • 2021 ), https://pfam.xfam.org/ ] predicts human DBNDD1 mainly as an intrinsically disordered protein (IDP) and also the recently released AlphaFold database (Jumper et al. (springer.com)
  • Unlike other dysbindin family proteins, DBNDD1 isoforms are probably non-classical secretory proteins (Talbot et al. (springer.com)
  • The 54-kD subunit of the signal recognition particle (SRP54) binds to signal sequences of nascent secretory and transmembrane proteins. (escholarship.org)
  • Researchers from the University of Sheffield found that while the proteins varied, there were structurally similar portions that interacted with the machinery. (diamond.ac.uk)
  • They occur in a large number of functionally diverse proteins mainly from eukaryotes. (embl.de)
  • ADP ribosylation factors (ARFs), which are members of the Ras superfamily of GTP-binding proteins, are critical components of vesicular trafficking pathways in eukaryotes. (embl.de)
  • The dysbindin domain-containing protein 1 (DBNDD1) is a conserved protein among higher eukaryotes whose structure and function are poorly investigated so far. (springer.com)
  • The dysbindin (dystrobrevin-binding protein) protein family is a group of evolutionarily related proteins of moderate size (Mw 13-45 kDa) in higher Eukaryotes. (springer.com)
  • The protein is targeted to the inner membrane surface and is released after contacting the SRP receptor protein FtsY. (nature.com)
  • This result and other findings suggested that Mgr2p controls the lateral release of inner membrane proteins harboring a stop-transfer signal that follows an N-terminal amino acid signal. (frontiersin.org)
  • This dual sorting mechanism is currently being investigated, and in yeast appears to be regulated by a recently discovered subunit, the Mgr2 protein. (frontiersin.org)
  • The precursor forms of prominent chloroplast proteins like the small subunit of stromal Rubisco (SSU), the thylakoid a/b binding protein (LHCP), or the thylakoid lumen localized subunits of the oxygen evolving complex of 23 kD and 33 kD (OE23, OE33) contain a phosphorylation site within the chloroplast targeting signal ( Waegemann and Soll 1996 ). (silverchair.com)
  • There has been controversy over the last 50 years in the UGT field with respect to the explanation for the phenomenon of latency: full UGT activity revealed by chemical or physical disruption of the microsomal membrane. (mdpi.com)
  • The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. (rndsystems.com)
  • The present article is based on the large amount of sequence data accumulated in these years and focuses on the viral nucleic acids and proteins and their function. (pdfroom.com)
  • This suggests that variants of other genes and proteins may also be involved in providing protection. (elifesciences.org)
  • Methods Probands from an AI cohort were screened by single molecule molecular inversion probes or targeted hybridisation capture (both a custom panel and whole exome sequencing) for COL17A1 variants. (bmj.com)
  • Results Nineteen unrelated probands with isolated AI (no co-segregating features) had 17 heterozygous, potentially pathogenic COL17A1 variants, including missense, premature termination codons, frameshift and splice site variants in both the endo-domains and the ecto-domains of the protein. (bmj.com)
  • However, unlike the other components of the harpoon, there are distinct variants of the TssA protein that contain radically different amino acid sequences at one end of the protein. (diamond.ac.uk)
  • CXCR1 is a class A, rhodopsin-like G-protein-coupled receptor (GPCR), the largest class of integral membrane proteins responsible for cellular signal transduction and targeted as drug receptors. (rcsb.org)
  • The team led by David Rice and Mark Thomas worked on a protein component of the harpoon called TssA which they already knew was an integral piece of the machinery. (diamond.ac.uk)
  • Many studies have shown that this protein is an integral part of the T6SS machinery, but research into the DNA that encodes this protein show that that between species, there is a lot of variation. (diamond.ac.uk)
  • We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
  • In bacteria, the function of the Rabbit Polyclonal to SAA4 tmRNA molecules is to release BMS-354825 cost ribosomes that have become stalled during protein synthesis and to tag incomplete and unnecessary peptides for proteolysis. (baxkyardgardener.com)
  • Nearly 39% of the ESTs were predicted to encode proteins of fungal origin and among these, 162 ESTs were predicted to contain N-terminal signal peptides (SPs) in their deduced open reading frames (ORFs). (biomedcentral.com)
  • Here, we present the backbone and side chain nuclear magnetic resonance assignments for the human DBNDD1 protein. (springer.com)
  • of480 bp, orf160 gene was cloned in CMS line, which located in the downstream of atp4 , its N-terminal was homologous with atp6 , and the C-terminal was homologous with nuclear sequence, and encoded 159 amino acids that were homologous with membrane proteins and cell cycle protein. (chinacrops.org)
  • Most chloroplastic proteins are nuclear encoded, synthesized in the cytosol, and posttranslationally imported into the organelle. (silverchair.com)
  • After the appressorium has been formed, a thin infection or penetration peg emerging from the base of the appressorium pierces the host cuticle and cell wall, and grows in between the plant cell wall and plasma membrane. (biomedcentral.com)
  • These hyphae are likely to interact with the host plasma membrane but pull away after plasmolysis (Figure 1A ), indicating either initial or weak host-pathogen association. (biomedcentral.com)
  • This situation differs from that of haustoria (analogous to primary hyphae) and the biotrophic invasive hyphae produced by obligate biotrophic fungi, such as powdery mildews and rusts, and other hemibiotrophs like Magnaporthe oryzae , the causal agent of rice blast, where the host plasma membrane remains adhered to fungal structures upon plasmolysis [ 2 ]. (biomedcentral.com)
  • Autotransporter proteins then remain in an unfolded state in the periplasm until they pass through the outer bacterial membrane, folding properly along the way. (anl.gov)
  • The nucleotide sequence of the cDNA obtained indicated that the 5' terminal 330 nucleotides differs from the published sequence of the ret proto-oncogene. (drugbank.com)
  • Nucleotide sequencing of the M gene of MHV-A59 and MHV-JHM (Armstrong et al. (pdfroom.com)
  • A single amino acid alteration of cysteine to serine within repeat 10 of PPR10 further drives dimerization of PPR10. (rcsb.org)
  • The ankyrin repeat is one of the most common protein-protein interaction motifs in nature. (embl.de)
  • Printed in Great Britain Key words: coronaviruses/genome expression/protein structure REVIEW ARTICLE Coronaviruses: Structure and Genome Expression By W. SPAAN, 1. (pdfroom.com)
  • The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. (cdc.gov)
  • Sequence analysis of the complete genome of SARS-CoV has shown an RNA molecule of about 29,750 bases in length, with a genome organization similar to that of other coronaviruses ( 9 - 11 ). (cdc.gov)
  • In addition, we have completely sequenced the viral genome of the SARS-CoV HSR1 and compared it to other SARS-CoV strains recently isolated in disease-epidemic areas. (cdc.gov)
  • This study characterized high-quality whole-genome sequences of a sentinel, surveillance-based collection of 1710 Neisseria gonorrhoeae (GC) isolates from 2019 collected in the USA as part of the Gonococcal Isolate Surveillance Project (GISP). (cdc.gov)
  • A protein [virus protein, genome-linked by a capsid architecture with 32 distinct cup-shaped depressions. (cdc.gov)
  • Abbreviations: VPg, virus protein, genome-linked. (cdc.gov)
  • Using potato virus X (PVX) based transient expression assays, we showed that one of the candidate effectors, i. e. contig 8 that encodes a cerato-platanin (CP) domain containing protein, unlike CP proteins from other fungal pathogens was unable to elicit a hypersensitive response (HR). (biomedcentral.com)
  • 2021 ). The human DBNDD1 isoforms 1 (UniProtKB: Q9H9R9-1) and 2 (UniProtKB: Q9H9R9-2) differ only in the N-terminal region where 20 amino acids are additional in isoform 2. (springer.com)
  • Analysis of the signal sequence binding activity of free SRP54 and SRP54M supports the conclusion that SRP54M binds signal sequences with lower affinity than the intact protein. (escholarship.org)
  • The subcellular localization of onion epidermal cells showed the green fluorescence on cell membranes, so the mitochondria-targeting carrier can be normally expressed. (chinacrops.org)
  • Isolation of ret proto-oncogene cDNA with an amino-terminal signal sequence. (drugbank.com)
  • In the present study we have isolated and sequenced an additional cDNA clone. (drugbank.com)
  • A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. (rupress.org)
  • A 16-kD protein fragment encoded by the cDNA was expressed in bacteria and used to immunize rabbits. (rupress.org)
  • They are involved in protein-protein interactions. (embl.de)
  • The cellular FLICE inhibitory protein (cFLIP) regulates both recruitment and processing of pro-caspase-8 within the DISC [ 13 ]. (biomedcentral.com)
  • The increase of the hydrophobicity in the signal sequences of E. coli presecretory proteins makes it possible to re-route the SecA dependent preproteins into the SRP targeting cycle 17 . (nature.com)
  • Expression of ompAI and ompAII separately in E. coli indicated that both genes could be independently transcribed from their own promoters and that both gene products were assembled into the E. coli outer membrane. (shengsci.com)
  • Recent structural determination of GPCRs has advanced by modifying the receptors with stabilizing mutations, insertion of the protein T4 lysozyme and truncations of their amino acid sequences, as well as addition of stabilizing antibodies and small molecules that facilitate crystallization in cubic phase monoolein mixtures. (rcsb.org)
  • HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
  • A 5.0-kb BamHI fragment containing the two genes in tandem was subcloned in pBluescript and used for further subcloning and sequencing of the genes. (shengsci.com)
  • This high-resolution snapshot of genetic profiles of 1710 GC sequences, through a comparison with 2018 data (1479 GC sequences) within the sentinel system, highlights change in proportions and expansion of select GC strains and the associated genetic mechanisms of resistance. (cdc.gov)
  • With cross-linking studies, it could be shown that the hydrophobicity of the signal sequence is crucial for SRP binding, since lowering the hydrophobicity resulted in less efficient cross-linking 15 , 16 . (nature.com)
  • This protein is found in a cellular compartment called the mitochondria, which is responsible for supplying energy to the rest of the cell and therefore plays an important role in metabolic processes. (elifesciences.org)
  • Using a gene editing tool to selectively substitute some of the building blocks, also known as amino acids, that make up the ATP5G1 protein revealed that improvements to the mitochondria were caused by switching specific amino acids. (elifesciences.org)
  • Indeed, most live cell applications of PTS utilize single split inteins for the purpose of N/C-terminal tagging 16 - 18 or manipulating protein assembly/expression 19 , 20 . (biorxiv.org)
  • A glycosylphosphatidylinositol (GPI) membrane anchor at the COOH-terminal tethers PrP to cell membranes. (biolegend.com)
  • Sequence analyses of unigenes revealed four potential groups: hydrolases, cell envelope associated proteins (CEAPs), candidate effectors and other proteins. (biomedcentral.com)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • Why don't all the proteins in the cell instead just stick to each other, aggregating into a big mess? (anl.gov)
  • Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. (cdc.gov)
  • The latter facilitates the simultaneous use of two orthogonal split inteins within the same peptide or protein, an approach termed tandem protein trans-splicing (tPTS). (biorxiv.org)
  • The receptor is in liquid crystalline phospholipid bilayers, without modification of its amino acid sequence and under physiological conditions. (rcsb.org)
  • The biochemical elucidation of the determinants for PPR10 dimerization may provide an important foundation to understand the working mechanisms of PPR proteins underlying their diverse physiological functions. (rcsb.org)
  • Furthermore, it can be cross-linked in intact chloroplasts to a high molecular weight complex containing both Toc and Tic subunits and a precursor protein. (silverchair.com)
  • A phosphorylated precursor protein can still bind to the chloroplast import machinery, but must become dephosphorylated before translocating through the membranes. (silverchair.com)
  • The 0 Å cross-linker CuCl 2 yields the reversible formation of disulfide bridge(s) between Toc64 and the established Toc complex subunits in purified outer envelope membranes. (silverchair.com)
  • Most variation between the two proteins was localized to the predicted surface loops and periplasmic turns, while the transmembrane strands and C-terminals domains were highly conserved. (shengsci.com)
  • The amino-terminal NG domain of SRP is bound to the ribosomal proteins uL23 and uL29, next to the tunnel exit and the carboxy-terminal M domain to the ribosomal 23S RNA 6 . (nature.com)
  • Taxonomic distribution of proteins containing ANK domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with ANK domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing ANK domain in the selected taxonomic class. (embl.de)
  • Crystal structure of the ARF-GAP domain and ankyrin repeats of PYK2-associated protein beta. (embl.de)
  • The crystal structure of the PAPbeta ARF-GAP domain and the C-terminal ankyrin repeats has been determined at 2.1 A resolution. (embl.de)
  • Human dysbindin domain-containing protein 1 (DBNDD1) is encoded by the gene DBNDD1 at chromosome locus 16q24.3. (springer.com)
  • As an outcome human DBNDD1 revealed a high sequence identity to dysbindin domain-containing proteins from other Hominidae (e.g. (springer.com)
  • Dysbindin domain-containing protein 1 (DBNDD1) is conserved in different species. (springer.com)
  • We also generated a MUC16 C-terminal domain (MUC16CTD) construct that was stably expressed in MUC16 negative SKOV3 cells. (biomedcentral.com)
  • For this work, they turned to the N-terminal passenger domain of the pertactin virulence protein from Bordetella pertussis, a typical autotransporter. (anl.gov)
  • The first step was to analyze the impact of the sequence of the amino acids in the passenger domain on the folding process. (anl.gov)
  • Griffithsin's antiviral activity stems from its ability to bind terminal mannoses present in high-mannose oligosaccharides and crosslink these glycans on the surface of the viral envelope glycoproteins. (mdpi.com)
  • In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
  • Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
  • Chemical or genetic engineering of proteins provides great potential to study protein function and pharmacology or to generate proteins with novel properties. (biorxiv.org)
  • could be used to identify and study other proteins that increase resilience to metabolic stress. (elifesciences.org)
  • The current study catalogues proteins putatively secreted at the in planta biotrophy-necrotrophy transition of C. truncatum . (biomedcentral.com)
  • The system involves the study of what are termed autotransporter proteins, which pathogenic bacteria secrete as virulence factors for infection. (anl.gov)
  • While all coronaviruses contains these proteins, a subset (HEV, HCV-OC43 and BCV) is now recognized to possess an additional glycopolypeptide (gp65), which is unrelated to S or M. N protein The number of amino acids in the N protein has been determined by cloning and sequencing for MHV strains A59 (Armstrong et al. (pdfroom.com)