• These toxins are 36-37 amino acids long and show high homology. (wikipedia.org)
  • The Open reading frame (ORF) of the AnpeNPV consists of 738 nucleotides encoding 245 amino acids with molecular masses of 29 kDa. (scirp.org)
  • The deduced amino acids were significant homol-ogy with other baculoviruses, such as Attacus ricini NPV (ArNPV) and Autographa californica NPV (AcNPV). (scirp.org)
  • By using sequence alignment of herpesviruses homologs, we identified conserved amino acids in the putative pUL105 ATP binding site and in the putative pUL70 zinc finger pattern. (frontiersin.org)
  • Mutational analysis of several of these amino acids both in pUL105 and pUL70, proved that they are crucial for viral replication. (frontiersin.org)
  • We also constructed, by homology modeling, a theoretical structure of the pUL105 N-terminal domain which indicates that the mutated conserved amino acids in this domain could be involved in ATP hydrolysis. (frontiersin.org)
  • To this end we use unsupervised learning to train a deep contextual language model on 86 billion amino acids across 250 million protein sequences spanning evolutionary diversity. (biorxiv.org)
  • The learned representation space has a multi-scale organization reflecting structure from the level of biochemical properties of amino acids to remote homology of proteins. (biorxiv.org)
  • The cDNA for CpHsp19.8 was 795 bp encoding 177 amino acids. (usda.gov)
  • The cDNA for CpHsp19.9 was 749 bp encoding 175 amino acids. (usda.gov)
  • The cDNA for CpHsp22.2 was 737 bp encoding 192 amino acids. (usda.gov)
  • Analysis of the protein sequences of the three CpHsps indicated the presence of 83 amino acids with homology to the a-crystallin domain. (usda.gov)
  • In bacteria, yeast, and fungi, the main role of this enzyme is limited to the utilization of exogenous D-amino acids (primarily D-Ala) [ 1 , 2 ]. (actanaturae.ru)
  • In higher eukaryotes - vertebrates and especially in mammals, the main role of DAAO is to maintain a certain level of D-amino acids, which are regulators of many important processes, primarily nervous activity. (actanaturae.ru)
  • The K a /K s ratio can be used to examine the action of natural selection on protein-coding regions, [5] [6] it provides information about the relative rates of nucleotide substitutions that change amino acids (non-synonymous substitutions) to those that do not change the encoded amino acid (synonymous substitutions). (wikipedia.org)
  • the alphabet is the 20 proteinogenic amino acids for proteins and the sense codons (i.e., the 61 codons that encode amino acids in the standard genetic code ) for aligned protein-coding gene sequences. (wikipedia.org)
  • In fact, substitution models can be developed for any biological characters that can be encoded using a specific alphabet (e.g., amino acid sequences combined with information about the conformation of those amino acids in three-dimensional protein structures [7] [8] ). (wikipedia.org)
  • Furthermore, using sequence analysis of human genomic DNA, researchers concluded that the amino acids important for catalysis by COX-1 are conserved and are equally important for catalysis by COX-2. (medscape.com)
  • from random sequences of amino acids is addressed. (lu.se)
  • ments of the amino acids along the protein chains. (lu.se)
  • To under- denoted the AB model, consists of chains of two kinds of stand the statistical distribution of hydrophobicity along proteins ``amino acids'' interacting with Lennard-Jones potentials. (lu.se)
  • These types of RNA help assemble protein building blocks (amino acids) into functioning proteins. (medlineplus.gov)
  • Alignment of the amino acid sequence of aprotinin with the Kunitz domains of human proteins allowed the identification and design of a family of peptides, named Angiopeps. (aspetjournals.org)
  • At the amino acid sequence level, human beta-Defensin 2 shares 98% and 51% homology with the proteins from rhesus monkey and rat, respectively. (rndsystems.com)
  • Its carboxyl-terminal portion (aa 180-251) shares only limited amino acid homology with other members of the FGF family of proteins. (quidel.com)
  • Computational methods bridge this sequence-annotation gap typically through homology-based annotation transfer by identifying sequence-similar proteins with known function or through prediction methods using evolutionary information. (rostlab.org)
  • Results -Several proteins ranging in molecular mass from 55 to 75 kd were unique to the hemolytic strain. (avma.org)
  • Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
  • Most commonly, this approach leverages high-resolution mass spectrometric measurements of proteolyzed proteins, combined with tandem peptide fragmentation to match observed spectra with those expected from their amino acid composition. (biorxiv.org)
  • Protein location can be predicted either from the sequence of a protein alone by identification of targeting peptide sequences and motifs, or by homology to proteins of known location. (biomedcentral.com)
  • A third approach, which is complementary, exploits the differences in amino acid composition of proteins associated to different cellular locations, and can be useful if motif and homology information are missing. (biomedcentral.com)
  • Calibration of the method using predicted values of amino acid exposure allows classifying proteins without 3D-information with an accuracy of 62% and discerning proteins in different locations even if they shared high levels of identity. (biomedcentral.com)
  • It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. (nih.gov)
  • Our analysis has been the amino acid sequences in proteins differ from what is carried out using two different methods, which differ substantially expected from random sequences in a statistically significant from what is used in ref. 3, although the starting point is similar. (lu.se)
  • PROT data base (6) of functional proteins, this method yields model containing only two amino acid types, hydrophobic and clear evidence for nonrandomness. (lu.se)
  • The results show that nonnative proteins are more structured and less solvent exposed than commonly believed. (lu.se)
  • Prediction of protein antigenic determinants from amino acid sequences. (scirp.org)
  • These embeddings originate from deep learned language models (LMs) for protein sequences (SeqVec) transferring the knowledge gained from predicting the next amino acid in 33 million protein sequences. (rostlab.org)
  • Oligonucleotide primers, designed on the basis of amino acid sequences of 2 tryptic peptides derived from 1 such protein and conserved regions of the C and B genes from members of the repeats in the structural toxin (RTX) family of bacterial toxins, were used to amplify cytotoxin-specific genes from M bovis genomic DNA. (avma.org)
  • However, even within one related group (bacteria, yeasts and fungi, mammals), DAAOs show very low homology between amino acid sequences. (actanaturae.ru)
  • Using a homology search, we obtained a set of candidate sequences, modelled the tertiary structure of the selected enzymes, and compared them with experimental and model structures of known DAAOs. (actanaturae.ru)
  • Currently, most protein sequences in databases are the result of translation of hypothetical transcripts derived from genomic sequencing data [ 3 ]. (biomedcentral.com)
  • As a result, gene duplication history can be inferred from the output independent of gene sequences. (biomedcentral.com)
  • Its gene encodes a 251 amino acid protein. (quidel.com)
  • Conclusions and Clinical Relevance -A gene was identified in M bovis that encodes a protein with sequence homology to other RTX toxins. (avma.org)
  • We propose an approach that uses bioinformatic methods in combination with general 3D structure and active center structure analysis to confirm that the gene found encodes D-amino acid oxidase and to predict the possible type of its substrate specificity. (actanaturae.ru)
  • Conversely, the transcription of COX-2 yields a 4.5-kb mRNA that encodes a 70-kd protein with roughly 70-75% homology to the COX-1 protein. (medscape.com)
  • The traditional phylogenetic principles and methods are based on amino acid or nucleotide sequence to assess homology, determine orthology and paralogy, and deduce relationships within gene families and/or reconstruct gene evolutionary events. (biomedcentral.com)
  • Exenatide is a synthetic version of exendin-4, a 39 amino acid peptide originally isolated from the venom of the Gila monster. (hindawi.com)
  • The amino-terminal portion of FGF-23 (aa 1-24) is hydrophobic and is likely to serve as a signal peptide allowing its secretion into the blood circulation. (quidel.com)
  • Gliadorphin is a 7 amino acid long peptide: Tyr-Pro-Gln-Pro-Gln-Pro-Phe which forms when the gastrointestinal system is compromised. (greenmedinfo.com)
  • Procedures -For male goats, results of serum biochemical analyses collected from 1992 through 2003 were retrieved from computerized records, as were signalment, clinical diagnoses, and discharge status. (avma.org)
  • Areas covered include sequence databases, pairwise and multiple sequence alignment, homology searches in sequence databases and subcellular localization prediction. (lu.se)
  • Here, we used CRISPR-mediated homology-directed repair and somatic cell nuclear transfer to produce a live calf with a six amino acid substitution in the BVDV binding domain of bovine CD46. (unl.edu)
  • The result showed that the bacterium expression system was suitable for the virus gene expression. (scirp.org)
  • Disruption of the ECA39 gene in yeast results in an increased growth rate in comparison to wild type, such that G1 is shorter. (bdbiosciences.com)
  • The result was a gene-edited calf with dramatically reduced susceptibility to infection as measured by reduced clinical signs and the lack of viral infection in white blood cells. (unl.edu)
  • Results of cytotoxin neutralization assays support the hypothesis that M bovis cytotoxin is encoded by this gene and belongs in the RTX family of bacterial exoproteins. (avma.org)
  • A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene. (nih.gov)
  • The traditional phylogeny analysis within gene family is mainly based on DNA or amino acid sequence homologies. (biomedcentral.com)
  • The results obtained with in vitro and in vivo models provided evidence for greater passage of aprotinin across the BBB than of holo-transferrin. (aspetjournals.org)
  • Results In vitro, PR3 promoted monocyte-derived MGC formation using cells from patients with GPA but not from patients with MPA, and this was dependent on soluble interleukin 6 (IL-6), as well as monocyte MAC-1 and protease-activated receptor-2, found to be overexpressed in the cells of patients with GPA. (bmj.com)
  • Another viral sequence encoded a protein with 70% amino acid homology to the predicted RdRp. (virology.ws)
  • The deduced amino acid sequence encoded by this open reading frame was homologous to RTX toxins. (avma.org)
  • An open reading frame encoding a 927-amino acid protein with a predicted molecular mass of 98.8 kd was amplified from M bovis genomic DNA. (avma.org)
  • PG2 integrates genome and transcriptome sequencing to incorporate protein variants containing amino acid substitutions, insertions, and deletions, as well as non-canonical reading frames, exons, and other variants caused by genomic and transcriptomic variation. (biorxiv.org)
  • Mutations in MYH9 result in the May-Hegglin anomaly, and Fechtner and Sebastian syndromes. (nih.gov)
  • Thus, our results suggest that mutations in MYH9 result in three megakaryocyte/platelet/leukocyte syndromes and are important in the pathogenesis of sensorineural deafness, cataracts and nephritis. (nih.gov)
  • Autosomal dominant hypophosphatemic rickets (ADHR), a rare genetic disorder, results from one of several different FGF-23 mutations that make the protein resistant to proteolytic cleavage. (quidel.com)
  • 35 mutations were silent so the strains differed in 8 amino acid only. (cdc.gov)
  • These bindings result in a disulfide pseudo-knot, characteristic for a class of toxins that contain the 'inhibitor cystine knot motif' (ICK). (wikipedia.org)
  • RESULTS: Here, we report the characterization of a cDNA, 1RT126I22, which contains a TNF homology domain. (usda.gov)
  • The capacity of certain E coli strains to produce Shiga toxins appears to have resulted from transduction of the responsible genes by bacteriophages. (medscape.com)
  • Here we expand this approach taking into account amino acid composition at different levels of amino acid exposure. (biomedcentral.com)
  • Eden A, Simchen G, Benvenisty N. Two yeast homologs of ECA39, a target for c-Myc regulation, code for cytosolic and mitochondrial branched-chain amino acid aminotransferases. (bdbiosciences.com)
  • As a result, reactive oxygen species easily damage mitochondrial DNA, causing cells to malfunction and ultimately to die. (medlineplus.gov)
  • Gliadin can be broken down into various amino acid lengths or peptides. (greenmedinfo.com)
  • When digestive enzymes are insufficient to break gliadorphin down into 2-3 amino acid lengths and a compromised intestinal wall allows for the leakage of the entire 7 amino acid long fragment into the blood, glaidorphin can pass through to the brain through circumventricular organs and activate opioid receptors resulting in disrupted brain function. (greenmedinfo.com)
  • This bleeding is painful and leads to long-term inflammation and deterioration of the joint (typically the ankles in children, and the ankles, knees, and elbows in adolescents and adults), resulting in permanent deformities, misalignment, loss of mobility, and extremities of unequal lengths. (medscape.com)
  • As a result, many (if not most) DAAO genes remain either unannotated or incorrectly annotated. (actanaturae.ru)
  • D-amino acid oxidase (DAAO, EC 1.2.1.2) plays an important role in the functioning of prokaryotes as well as of lower (yeast and fungi) and higher eukaryotes (mammals). (actanaturae.ru)
  • So the homology of WN-Volgograd-4 and WN-Romania-1996M strains was 99.77% for aa sequence and 99.58% for nt sequence. (cdc.gov)
  • By contrast, high levels of Gap1 expression under sev enhancer/promoter control using amplification by the UAS/GAL4 system in an otherwise wild type background results in a rough eye phenotype due to loss of photoreceptors (Rørth, 1996). (sdbonline.org)
  • As a result, extant angiosperms vary more than 1000-fold in genome sizes and nearly 50-fold in chromosome numbers [ 2 ]. (biomedcentral.com)
  • He has contributed significantly to the state-of-the-art by frequently presenting novel ideas, methods and results and have pioneered several important proof-of-concepts relevant for the project, including the conceptualization of DNA, RNA and protein variation to Variation Ontology (Vihinen, Genome Res . (lu.se)
  • Pleckstrin homology domain. (embl.de)
  • This damage results from a buildup of harmful molecules called reactive oxygen species, which are byproducts of energy production in mitochondria. (medlineplus.gov)
  • The trout TNFSF 13b predicted protein shares 54% amino acid identity with human and chicken TNFSF 13b, and 51% identity with mouse TNFSF 13b. (usda.gov)
  • Mouse PlGF shares 65% amino acid identity with human PlGF-2. (reliatech.de)
  • The method reaches an accuracy of 68% when using as input 3D-derived values of amino acid exposure. (biomedcentral.com)
  • Results:- Time of exposure is 0-5 years and in most of the cases the forced expiratory volume in one second is 70% + 5 but peak expiratory flow rate is markedly decreased which shows that lung functions are deteriorated to a considerable extent. (who.int)
  • Spontaneous mutation and acquired immunologic processes can result in this disorder, as well. (medscape.com)
  • Minor differences might arise as a result of 'genetic noise' or adaptation to host or vector. (cdc.gov)
  • This condition results from a combination of genetic, environmental, and lifestyle factors, many of which have not been identified. (medlineplus.gov)
  • However, native GLP-1 is degraded rapidly by the ubiquitous enzyme dipeptidyl peptidase-4 (DPP-4), resulting in a half-life of no more than 1~2 minutes [ 10 ], which limits its therapeutic usefulness in clinic. (hindawi.com)
  • The amino-terminal antibody is biotinylated for capture and the carboxyl-terminal antibody is conjugated with the enzyme horseradish peroxidase (HRP) for detection. (quidel.com)
  • D-amino acid oxidase is increasingly used in various fields, which requires the development of new variants of the enzyme with specific properties. (actanaturae.ru)
  • Regardless of the etiology, a deficiency of cyclooxygenase (COX), a key regulatory enzyme in the synthetic pathway of eicosanoid production, results in beneficial and detrimental physiologic conditions relative to imbalances of the eicosanoids. (medscape.com)
  • E. B. Carstens, A. Krebs, Gallerneault, C. E. (1986) Identifica-tion of an amino acid essential to the normal assembly of Auto-grapha californica nuclear polyhedrosis virus polyhedra. (scirp.org)
  • These results clearly show that at least two related but distinct RNA viruses are present in Nymph Lake. (virology.ws)
  • There are more than one record in the search results.Please specify issue. (go.jp)
  • The inhibition of inactivation results in excessive sodium influx. (wikipedia.org)
  • Furthermore, ECA39 is expressed in c-myc induced tumors and displays significant homology to the prokaryotic branched-chain amino acid aminotransferase (BCAT). (bdbiosciences.com)