AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Peptide MappingPeptidesPeptide FragmentsAmino AcidsChromosome MappingAmino Acid SequenceMolecular WeightTrypsinMolecular Sequence DataElectrophoresis, Polyacrylamide GelSequence Homology, Amino AcidPeptide LibraryChromatography, High Pressure LiquidBase SequenceBinding SitesCloning, MolecularAntimicrobial Cationic PeptidesKineticsPeptides, CyclicProtein BindingCyanogen BromideProtein ConformationEpitope MappingMass SpectrometryEpitopesCell LineRecombinant ProteinsEscherichia coliOligopeptidesModels, MolecularPeptide HydrolasesCattlePeptide BiosynthesisMutationSubstrate SpecificityAmino Acid SubstitutionStructure-Activity RelationshipPhosphorylationProtein PrecursorsPhosphopeptidesProteinsSequence AlignmentViral ProteinsMacromolecular SubstancesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationProtein Structure, SecondaryAntibodies, MonoclonalProtein Structure, TertiaryRabbitsIsoelectric FocusingSpecies SpecificityMutagenesis, Site-DirectedEndopeptidasesNatriuretic Peptide, BrainDisulfidesProtein Processing, Post-TranslationalBacterial ProteinsChromatography, Ion ExchangeRestriction MappingProtein BiosynthesisChromatography, GelChromatography, AffinityCysteineSerine EndopeptidasesLysineProtein KinasesChymotrypsinRNA, MessengerMembrane ProteinsDNACross ReactionsGlycoproteinsCells, CulturedAmino Acid MotifsRecombinant Fusion ProteinsSequence Homology, Nucleic AcidChickensVasoactive Intestinal PeptideIsoenzymesCalcitonin Gene-Related PeptideHydrolysisCell-Penetrating PeptidesIsoelectric PointCell MembraneCarrier ProteinsBrain MappingElectrophoresis, Gel, Two-DimensionalAffinity LabelsSequence AnalysisGlycosylationCricetinaeSerineDNA, ComplementaryBrain ChemistryPhysical Chromosome MappingGenesSwineLeucinePeptide YYImmunosorbent Techniques
ProteinsAntibodiesSequenceEpitopeEpitopesResiduesTogether by peptide bondsSequencesJoined amino acidsCharacterizeCollagen peptidesOverlapping peptide librariesTryptic peptidePolypeptidesConformationalProtein or peptideSynthesisCustom peptideMoleculeSyntheticEnzymesKinaseCleavageNonribosomalMicroarraysProteasesTarget aminoChemistryBondsTrypsinSubstrateHydrophobicAntigenicAtomsChainsMassLigandImmuneCarboxylTerminusProteomicsOxidationAbsorptionMotifTumorCellsHydrationBlocking
Proteins19
  • Peptides and proteins are chains of amino acids held together by peptide bonds (and sometimes by a few isopeptide bonds). (wikipedia.org)
  • Organisms use enzymes to produce nonribosomal peptides, and ribosomes to produce proteins via reactions that differ in details from dehydration synthesis. (wikipedia.org)
  • The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
  • Figure 1: The amino acid sequences for three small proteins. (customuniversitypapers.com)
  • Polyclonal antibodies are derived from immunization of rabbits, goats, and sheep using peptides, recombinant proteins, or small chemical molecules conjugated to immune stimulating molecules to generate specific immune responses. (avivasysbio.com)
  • Aviva Systems Biology offers a broad selection of polyclonal antibodies in purified forms to recognize proteins, peptides, small molecules, and other substances for a variety of experimental applications. (avivasysbio.com)
  • Two dimensional phosphotryptic peptide maps of the human bcl-3 and the human 37-kD (I kappa B alpha) proteins reveal that the phosphopeptides from the 37-kD (I kappa B alpha) protein are nested within the bcl-3 protein. (duke.edu)
  • Copper ions bind to biomolecules (e.g., peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. (mdpi.com)
  • The identities of the peptides attributed to selected gluten proteins were confirmed using MS/MS with BioMultiView to match the predicted and measured partial amino acid sequences. (unboundmedicine.com)
  • Research in our laboratory focuses on the structural studies of plant proteins, peptides and natural products. (cimap.res.in)
  • Major ongoing research activities are, (i) Crystallographic structural studies of plant proteins, especially enzymes involved in plant secondary metabolite pathways (ii) Developing co-crystallization methods to enhance the bio-availability of natural product drug candidates (iii)Structure and folding of synthetic polypeptides containing non-natural amino acids (iv) Mass spectrometric identification and sequencing of biologically active plant peptides in medicinal and aromatic plants (MAPs). (cimap.res.in)
  • Peptides, proteins - they're pretty easy to confuse. (gainful.com)
  • However, peptides and proteins are not exactly the same thing - so what's the difference? (gainful.com)
  • Peptides benefit the body in similar ways to proteins, but they are smaller than proteins, so they are even easier for the body to absorb and use. (gainful.com)
  • Animal or plant sources of proteins like meat, dairy, fish and shellfish, beans and lentils, whole grains, soy and eggs all have peptides. (gainful.com)
  • however, proteins are longer chains of amino acids that are tied together by peptide bonds. (gainful.com)
  • As you know, proteins are made of many amino acids. (gainful.com)
  • Collagen peptides are made by breaking down whole collagen proteins into smaller pieces. (medlineplus.gov)
  • Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)
Antibodies10
  • For indirect mapping, peptide-specific rabbit antibodies were raised to block IgE against surface-exposed epitopes on folded Art v 1. (nih.gov)
  • Aviva's flagship polyclonal antibodies, designated with a beginning code of ARP or AVARP, were generated using small peptide immunogens which allow you to precisely map protein structure in experiments and monitor protein function in a cellular context. (avivasysbio.com)
  • Whether it is a post-translational modification, a conjugate for ELISA or immunofluorescence, amino, carboxyl termini or the middle of our 15,000+ protein and non-protein targets, our antibodies are directed against human, mouse, rat and other species to provide deep coverage of proteomic space. (avivasysbio.com)
  • The epitope mapping that Integral Molecular has done has greatly supported both our mechanistic understanding and the intellectual property claims of our proprietary antibodies. (integralmolecular.com)
  • OBJECTIVE: To distinguish the difference among the pathogenesis of 60 000 SSA antigen mono-antigen peptides (MAPs), and to discuss the nosogenesis of anti-60 000 SSA antibodies in correlative rheumatic diseases. (elsevierpure.com)
  • ELISA against recombinant 60 000 SSA antigen MAPs were done to detect anti-MAPs antibodies in 59 sera with anti-SSA antibodies, and analyzing the relations of anti-MAPs antibodies and organism injuries. (elsevierpure.com)
  • anti-MAP(6) antibodies have negative relation to heart lesion. (elsevierpure.com)
  • CONCLUSIONS: The appearance of some anti-MAPs antibodies implies the lesions of some organs, while the appearance of some other anti-MAPs antibodies have the protection to some organs. (elsevierpure.com)
  • We concluded that it is maybe the different anti-MAPs antibodies that result in different clinical manifestations. (elsevierpure.com)
  • CONCLUSION: In FMDV type C, amino acid substitutions seen in mutants that are resistant to neutralization by monoclonal antibodies (MAbs) map to predominantly surface-oriented residues with solvent-accessible side-chains not involved in interactions with other amino acids, whereas residues which are accessible but not substituted are found to be more frequently involved in protein-protein interactions. (ox.ac.uk)
Sequence11
  • Four overlapping peptides incorporating surface-exposed amino acids representing the full-length Art v 1 sequence were synthesized and used to search for IgE reactivity to sequential epitopes. (nih.gov)
  • The metal abstraction peptide (MAP) tag is a tripeptide sequence capable of abstracting a metal ion from a chelator and binding it with extremely high affinity at neutral pH. (ku.edu)
  • The armadillo (Arm) repeat is an approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila melanogaster segment polarity gene armadillo involved in signal transduction through wingless. (embl.de)
  • A core region of beta-catenin, composed of 12 copies of a 42 amino acid sequence motif known as an armadillo repeat, mediates these interactions. (embl.de)
  • You can block the antibody by the specific target amino acid sequence of peptide. (blockingantibody.com)
  • The function of a protein depends on its three-dimensional shape, which in turn depends on the protein's primary structure = its amino acid sequence. (customuniversitypapers.com)
  • If polypeptides from closely related species have the same amino acid sequence with the same amino acid residues at the same location, then treatment with the same enzyme should lead to synthesis of similar length fragments. (customuniversitypapers.com)
  • METHODS The anti-CTLA4 monoclonal antibody was characterized by liquid-mass technique from the aspects of intact molecular weight, subunit molecular weight, amino acid sequence coverage, disulfide bond, N -linked glycosylation site and glycoform. (magtechjournal.com)
  • METHODS: MAPs were artificially synthesized according to the amino acid sequence of 20 positive epitopes and 1 control segment of 60 000 SSA antigen. (elsevierpure.com)
  • To do so, we mutated the proteolytically revealed TL sequence of rPAR 2 and examined the impact on stimulating intracellular calcium transients and mitogen-activated protein (MAP) kinase. (aspetjournals.org)
  • Turn any protein sequence into a linear or conformational peptide librarie. (pepperprint.com)
Epitope6
Epitopes4
  • Mugwort-allergic patients and humanized mice sensitized by allergen inhalation showed IgE reactivity and/or basophil activation mainly to folded, complete Art v 1 but not to unfolded, sequential peptide epitopes. (nih.gov)
  • Peptides short amino acid chains or epitopes or blocking antagonists. (blockingantibody.com)
  • Map T-cell epitopes and monitor cellular immune responses. (pepperprint.com)
  • Using PEPperPRINT's unique laser printing technology, entire protein targets can be translated into highly overlapping peptide libraries in a convenient microarray format, allowing for both in-depth and high-throughput screening of immunogenic epitopes. (pepperprint.com)
Residues8
  • Tertiary structure is formed by interactions among amino acid side chains and involves weak interactions including hydrophobic / hydrophilic interactions and hydrogen bonds, ionic bonds, and covalent bonds such as disulfide bridges formed between two cysteine residues. (customuniversitypapers.com)
  • Integral Molecular was able to identify critical binding residues for an antibody for which no mapping had been successfully done after years in development. (integralmolecular.com)
  • In practice, this parameter is defined by the number of amino acid residues shared by neighboring peptides. (proteogenix.science)
  • In contrast, when offset numbers are low and peptides short, the resulting library will have more peptides, making it easier to identify the interface residues but also resulting in more time-consuming experiments. (proteogenix.science)
  • First, the major histocompatibility complex (MHC) and T-cell receptor (TCR) contact residues were identified by analysis of single site substituted analogue peptides for HLA-DR4 binding and cognate T cell recognition using both T hybridomas and polyclonal T cells from peptide-immunized HLA-DR4 transgenic mice. (biomedcentral.com)
  • The amino acids Ala268, Ser269, Glu271 and Thr272 constituted the major TCR contact residues, as substitution at these positions did not affect HLA-DR4(B1*0401) binding but abrogated T cell responses. (biomedcentral.com)
  • The amino acid distributions, at all positions in neoepitopes of all lengths, contain more hydrophobic residues than the wild-type sequences. (lifetein.com)
  • The results of cDNA cloning, peptide mapping, and amino acid sequencing of PPAE revealed that PPAE is synthesized as prepro-PPAE with 441 amino acid residues and is activated from pro-PPAE by cleavage of a peptide bond between Lys152 and Ile153. (lookformedical.com)
Together by peptide bonds1
Sequences7
  • That means their amino acid sequences are highly conserved. (customuniversitypapers.com)
  • Proteases are enzymes that cut (cleave) polypeptides at specific amino acid sequences. (customuniversitypapers.com)
  • Removal of the amino-terminal sequences of the bcl-3 protein generates a protein that inhibits the DNA binding of the p50-p65 heterodimer but, like the 37-kD (I kappa B alpha) protein, is no longer able to inhibit the binding of the p50 and p49 homodimers with kappa B DNA. (duke.edu)
  • We evaluated the ability of different trypsin-revealed tethered ligand (TL) sequences of rat proteinase-activated receptor 2 (rPAR 2 ) and the corresponding soluble TL-derived agonist peptides to trigger agonist-biased signaling. (aspetjournals.org)
  • Identify and characterize antibody binding sequences with amino acid resolution. (pepperprint.com)
  • The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
  • The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
Joined amino acids1
Characterize1
  • Control peptides were used to further characterize the chirality of the final nickel-peptide complex, and DFT calculations were performed to validate the hypothesized position of the chiral inversions. (ku.edu)
Collagen peptides15
Overlapping peptide libraries1
  • Identify single amino acid effects with maximally overlapping peptide libraries. (pepperprint.com)
Tryptic peptide2
  • Comparison of tryptic peptide maps of the bcl-3 protein synthesized in vitro, and p56 and p38 from HeLa cells, shows that they are all structurally related. (duke.edu)
  • iii), identify exposed regions of the intact and truncated TIMP-1 molecules by measuring the rate of tryptic peptide release and (iv), locate sites of aberrant proteolysis seen when recombinant human TIMP-1 was purified at large scale. (kent.ac.uk)
Polypeptides1
  • till polypeptides that are long, continuous, and unbranched synthetic peptide chains. (blockingantibody.com)
Conformational4
  • In living organisms, the process is normally catalyzed by enzymes known as peptidases or proteases, although there are reports of peptide bond hydrolysis caused by conformational strain as the peptide/protein folds into the native structure. (wikipedia.org)
  • A nonnative isomer of some peptide groups can disrupt the conformational folding significantly, either slowing it or preventing it from even occurring until the native isomer is reached. (wikipedia.org)
  • Blocking of allergic patients' IgE with peptide-specific rabbit antisera identified a hitherto unknown major conformational IgE binding site in the C-terminal Art v 1 domain. (nih.gov)
  • Identification of the new major conformational IgE binding site on Art v 1, which can be blocked with IgG raised against non-IgE reactive Art v 1 peptides, is an important basis for the development of a hypoallergenic peptide vaccine for mugwort allergy. (nih.gov)
Protein or peptide1
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
Synthesis5
  • The amide bond is synthesized when the carboxyl group of one amino acid molecule reacts with the amino group of the other amino acid molecule, causing the release of a molecule of water (H2O), hence the process is a dehydration synthesis reaction. (wikipedia.org)
  • These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. (blockingantibody.com)
  • The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs. (blockingantibody.com)
  • With ProteoGenix's cost-effective method of peptide library synthesis , you can now employ high-quality peptides for a fraction of the cost. (proteogenix.science)
  • Why choose ProteoGenix for overlapping peptide library synthesis? (proteogenix.science)
Custom peptide1
Molecule4
  • Proteolytic events included removal of a peptide from the surface of the precursor molecule that connected the NH2- and COOH-termini of the mature protein. (rupress.org)
  • The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. (evidentic.com)
  • This procedure accounted for the entire molecule, except two short peptides of 2 and 4 amino acids in length. (kent.ac.uk)
  • Peptide binding to a Human leukocyte antigen (HLA) molecule is a requirement for raising adaptive immunity. (lifetein.com)
Synthetic4
  • LTD by dopamine + 1 S ,3 R -ACPD coapplication was also blocked by postsynaptic injection of synthetic MAP-K substrate peptide. (jneurosci.org)
  • Peptide libraries are collections of synthetic peptides, invaluable for studying protein-protein interactions. (proteogenix.science)
  • Furthermore, the TL-derived synthetic peptide SLAAAA-NH 2 failed to cause PAR 2 -mediated calcium signaling but did activate MAP kinase, whereas SLIGRL-NH 2 triggered both calcium and MAP kinase signaling by all receptors. (aspetjournals.org)
  • Thus, PAR 2 can signal to multiple pathways that are differentially triggered by distinct proteinase-revealed TLs or by synthetic signal-selective activating peptides. (aspetjournals.org)
Enzymes6
  • Some peptides, like alpha-amanitin, are called ribosomal peptides as they are made by ribosomes, but many are nonribosomal peptides as they are synthesized by specialized enzymes rather than ribosomes. (wikipedia.org)
  • For example, the tripeptide glutathione is synthesized in two steps from free amino acids, by two enzymes: glutamate-cysteine ligase (forms an isopeptide bond, which is not a peptide bond) and glutathione synthetase (forms a peptide bond). (wikipedia.org)
  • Both of these mechanisms for lowering the activation energy have been observed in peptidyl prolyl isomerases (PPIases), which are naturally occurring enzymes that catalyze the cis-trans isomerization of X-Pro peptide bonds. (wikipedia.org)
  • Cooperative phosphorylation at these sites by both enzymes was observed in vitro and reflected in vivo by the predominance of the peptide phosphorylated on both sites, as opposed to singly phosphorylated peptides. (nih.gov)
  • Analysis of inactivated enzymes by peptide mapping and isoelcetric focusing have revealed that more than one inactive enzyme species is formed with each inactivator. (bl.uk)
  • Natural products classed as polyketides, nonribosomal peptides and hybrids of both are often biosynthesized by giant, complex enzymes known as polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). (iucr.org)
Kinase7
  • Consistently, LTD by dopamine + 1 S ,3 R -ACPD coapplication, as well as the two other forms of LTD (LTD by dopamine + tetani and LTD by 1S,3R-ACPD + tetani), was blocked by bath application of MAP-K kinase inhibitor PD98059. (jneurosci.org)
  • We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kDa ribosomal S6 kinase (RSK) and mitogen-activated protein kinase (MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. (nih.gov)
  • The major phosphopeptides derived from biosynthetically labeled c-Fos correspond to phosphopeptides generated after phosphorylation of c-Fos in vitro with both RSK and MAP kinase. (nih.gov)
  • The phosphorylation sites identified for RSK (Ser-362) and MAP kinase (Ser-374) are in the transrepression domain. (nih.gov)
  • This study suggests a role for nuclear RSK and MAP kinase in modulating newly synthesized c-Fos phosphorylation and downstream signaling. (nih.gov)
  • Upon trypsin activation, all constructs stimulated MAP kinase signaling, but only the wt-rPAR 2 and rPAR 2 -Ala 39-42 triggered calcium signaling. (aspetjournals.org)
  • The peptides AAIGRL-NH 2 and LSIGRL-NH 2 triggered neither calcium nor MAP kinase signals. (aspetjournals.org)
Cleavage2
  • The chase data suggest the occurrence of a simultaneous cleavage and formation of a peptide bond, raising the possibility that annealment of the fragments to give rise to the mature subunit involves a transpeptidation event rather than cleavage and subsequent religation. (rupress.org)
  • The presence of a particular BoNT serotype is demonstrated by mass spectrometric detection of the peptide cleavage products corresponding to their specific toxin-dependent location. (cdc.gov)
Nonribosomal1
  • Indanomycin is biosynthesized by a hybrid nonribosomal peptide synthase/polyketide synthase (NRPS/PKS) followed by a number of `tailoring' steps to form the two ring systems that are present in the mature product. (iucr.org)
Microarrays3
  • How do PEPperCHIP ® Peptide Microarrays work? (pepperprint.com)
  • Learn more about the research applications of PEPperCHIP ® Peptide Microarrays in this short video. (pepperprint.com)
  • Learn how to perform our assays in your own lab, or discover how other scientists have implemented PEPperCHIP® Peptide Microarrays in their research. (pepperprint.com)
Proteases1
  • A highly specific protease, trypsin, is used to cleave the protein into suitably sized peptides, although a combination of multiple proteases may also be used to generate more complete PTM site information. (sigmaaldrich.com)
Target amino1
Chemistry1
  • In organic chemistry, a peptide bond is an amide type of covalent chemical bond linking two consecutive alpha-amino acids from C1 (carbon number one) of one alpha-amino acid and N2 (nitrogen number two) of another, along a peptide or protein chain. (wikipedia.org)
Bonds5
  • The hydrolysis of peptide bonds in water releases 8-16 kJ/mol (2-4 kcal/mol) of Gibbs energy. (wikipedia.org)
  • The trans form is preferred overwhelmingly in most peptide bonds (roughly 1000:1 ratio in trans:cis populations). (wikipedia.org)
  • The digestive enzyme chymotrypsin, for example, cuts peptide bonds formed from the carbonyl groups of tryptophan, phenylalanine, and tyrosine. (customuniversitypapers.com)
  • Another protease, papain (derived from Papaya) cuts peptide bonds formed from leucine and glycine. (customuniversitypapers.com)
  • All of the five most carboxyl-terminal cysteines are used to form inter-chain disulfide bonds in dimers and higher multimers, while the four amino-terminal cysteines are not. (lu.se)
Trypsin1
  • The filter membrane retains the mAb of interest until the digestion procedure is complete, following which peptides can pass through the filter while excluding trypsin. (sigmaaldrich.com)
Substrate1
  • This method can detect all seven known BoNT serotypes and involves incubating BoNT with a peptide substrate that mimics each toxin's natural in vivo neuronal protein target. (cdc.gov)
Hydrophobic2
  • However, the activation energy can be lowered (and the isomerization catalyzed) by changes that favor the single-bonded form, such as placing the peptide group in a hydrophobic environment or donating a hydrogen bond to the nitrogen atom of an X-Pro peptide group. (wikipedia.org)
  • Outside the core domain a short hydrophobic region containing four seven-amino acid repeats (consensus: APVTYGA) was identified near the carboxy terminus of the 80-kD articulin, but near the amino terminus of the 86-kD articulin. (rupress.org)
Antigenic4
  • So the antigenic peptides can be detected by T cells. (lifetein.com)
  • These mutations are passenger mutations and the corresponding antigenic peptides are unique to the tumors in which they were identified. (lifetein.com)
  • There are significant changes in the structure of other antigenic loops and in some internal regions involved in protomer-protomer contacts, including the entire amino-terminal portion of VP2, described here for the first time for a picornavirus. (ox.ac.uk)
  • Antigenic sites have been identified by genetic and peptide mapping methods, and located on the capsid. (ox.ac.uk)
Atoms2
  • However, X-Pro peptide groups tend to have a roughly 30:1 ratio, presumably because the symmetry between the Cα and Cδ atoms of proline makes the cis and trans isomers nearly equal in energy, see the figure. (wikipedia.org)
  • Following incubation of the nickel-containing peptide in D2O and back-exchange into H2O, incorporation of deuterium into non-exchangeable positions is observed, indicating chiral inversion occurs at two of the alpha carbon atoms in the peptide. (ku.edu)
Chains1
  • The peptide containing the N -linked glycosylations site is EEQYNSTYR, and the N -glycosylation site is located at Asn298 of the heavy chains. (magtechjournal.com)
Mass6
  • However, motif redundancy among detected peptides by mass spectrometry (MS) leads to errors and ambiguities in the analysis. (biorxiv.org)
  • The use of a high-resolution mass spectrometry-based approach for the characterization is commonly referred to as a multi-attribute method (MAM) 1 , as the peptide mapping analysis yields multiple pieces of information about the protein in a single analysis. (sigmaaldrich.com)
  • Peptide maps of tryptic digests of HPLC fractions were obtained from a tandem quadrupole time-of-flight mass spectrometer (MALDI QqTOF MS) and were submitted to the ProFound search engine. (unboundmedicine.com)
  • Creatine peptides help support muscle strength, short energy bursts, the power output of muscle, recovery and increases in lean body mass. (gainful.com)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • The Mass Distance Fin- gerprint is calculated from high accuracy measured peptide masses. (lu.se)
Ligand1
  • This tumor-specific peptide could be a potent and selective ligand for FRα. (lifetein.com)
Immune5
  • Amino acid substitutions can yield neoantigens that are detected by the immune system. (lifetein.com)
  • Since neoantigens are small peptides harboring tumor mutations, immunization with them usually needs strong immunostimulatory agents to produce an effective immune response. (lifetein.com)
  • Peptides as vaccines may not be able to stimulate the immune system powerfully enough on their own. (lifetein.com)
  • Different patients have different immune response to MAPs. (elsevierpure.com)
  • Peptides have a number of benefits for the skin, weight, immune system - even muscles. (gainful.com)
Carboxyl1
  • One loses a hydrogen and oxygen from its carboxyl group (COOH) and the other loses a hydrogen from its amino group (NH2). (wikipedia.org)
Terminus1
  • We showed recently that CTCF binds to cohesin through a conserved YxF motif in the amino terminus of CTCF. (cbinsights.com)
Proteomics1
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
Oxidation3
  • Peptide mapping, a bottom-up approach to protein characterization, enables the profiling of a protein's primary structure as well as its post-translational modifications (PTMs) such as oxidation, deamidation, and glycosylation. (sigmaaldrich.com)
  • In addition, minimizing protein degradation during sample preparation, such as amino acid oxidation and deamidation, is also crucial in obtaining an accurate depiction of the therapeutic protein. (sigmaaldrich.com)
  • The method uses filter-assisted sample preparation (FASP) 3 , along with a proprietary buffer solution that minimizes amino acid oxidation and deamidation. (sigmaaldrich.com)
Absorption2
  • The wavelength of absorption for a peptide bond is 190-230 nm, which makes it particularly susceptible to UV radiation. (wikipedia.org)
  • Oral Ingestion of AP Collagen Peptide Leads to Systemic Absorption of Gly-Pro-Hyp, Alleviating HO-Induced Dermal Fibroblast Aging. (medlineplus.gov)
Motif2
  • In addition motif redundancy can affect quantitative and statistical analysis and prevent a realistic comparison of peptide numbers between datasets. (biorxiv.org)
  • One approach to achieving a non-redundant quantitation of phospho-motifs is to quantify each peptide but only assign a reference peptide, according to some quality criteria, as the quantification of the motif. (biorxiv.org)
Tumor4
  • Adalimumab is a recombinant human IgG1 mAb glycoprotein of 1330 amino acids specific for human tumor necrosis factor (TNF). (sigmaaldrich.com)
  • This tumor-targeting peptide was proved by both phage homing experiment and fluorescence imaging. (lifetein.com)
  • 2. The peptide was injected intravenously into a tumor-bearing nude mouse. (lifetein.com)
  • Check the cancer peptide database for a list of tumor peptides. (lifetein.com)
Cells3
  • An FRα binding peptide C7 (Met-His-Thr-Ala-Pro-Gly-Trp-Gly-Tyr-Arg-Leu-Ser, MHTAPGWGYRLS) was found to bind to FRα expressing cells. (lifetein.com)
  • 2. FITC conjugated peptide was incubated with the cells for 4 hours at 37 °C. (lifetein.com)
  • Creatine peptides enhance energy production in your cells. (gainful.com)
Hydration1
  • This distribution comprises a small fraction of protein-specific hydration sites, where water rotation is strongly retarded, and a dominant fraction of generic hydration sites, where water rotation is as fast as in the hydration shells of small peptides. (lu.se)
Blocking1