The way they fold depends in part on the way the amino acids interact with each other. (nih.gov)
Scientists are beginning to solve that puzzle with research into how amino acids interact, and they are using powerful new computer programs that help predict protein motifs. (nih.gov)
The receptor can be activated by a range of simple amino acids including glycine , β-alanine and taurine, and can be selectively blocked by the high-affinity competitive antagonist strychnine . (bionity.com)
Approximately 100 amino acids, corresponding to the middle region of class I/II HCPs, are absent, although N- and C-terminal regions and residues for metallocluster ligands are conserved among all HCP classes. (nature.com)
posttranslational modifications, amino acid variations, computational mutation analysis, protein PTM predictor, network biology Introduction Protein PTMs are biochemical alterations of amino acids that change the physicochemical properties of target proteins, leading to structural changes and therefore regulating protein-protein interactions and cellular signal transduction in developmental and cancer pathways [1]. (deepdyve.com)
The Multiple allignments of the selected sequences revealed two highly conserve motif regions of amino acids that enabled us to design a degenerate primers. (ac.ir)
The polyclonal antiserum reacts with human and mouse cyclin C. A polypeptide fragment containing amino acids 290-303 (QGPNGSQNSSYSQS) at the C-terminus of human cyclin C, with the addition of a C-terminal cysteine (C) residue to facilitate conjugation to KLH, was used as immunogen. (bdbiosciences.com)
Full-length ChChd3 has 227 amino acids ARP 100 with an N-terminal myristoylation motif followed by a DUF737 domain (domain of unknown function) and a coiled-coil helix-coiled-coil helix (chch) domain (Fig. 1 and amino acid sequence and exon organization of ChChd3. (sciencepop.org)
These changes in amino acids affect properties of proteins-such as hemagglutinin (HA) or neuraminidase (NA), the surface proteins of influenza viruses. (cdc.gov)
Genetic changes in amino acids-the substitution of one amino acid for another-can affect the properties of a virus such as how well a virus grows, how well it transmits between hosts or its susceptibility to antiviral drugs. (cdc.gov)
These are identical through amino acid 727, but then diverge, with hGRα having an additional 50 amino acids and hGRβ having an additional, nonhomologous 15 amino acids. (medscape.com)
The NTD of the hGRα contains a major transactivation domain, termed activation function (AF)-1, which is located between amino acids 77 and 262 of the hGRα and is ligand-independent. (medscape.com)
This review aims to link recent molecular data, often translated into amino acid sequences and predicted three dimensional structural motifs, to known mechanical properties. (bioone.org)
A putative late gene promoter motif sequence, AGTAT was found in the 3' upstream sequences of these genes. (umass.edu)
The retrieved sequences from Genbank amino acid sequence databases were analyzed for designing degenerate primers. (ac.ir)
Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
Availability of genome sequences facilitated the identification of pneumococcal surface proteins bearing characteristic motifs such as choline-binding proteins (Cbp) and peptidoglycan binding (LPXTG) proteins. (biomedcentral.com)
Pauling believed that protein shapes-independently of amino acid sequences-determined antibody specificity and biological specificity in general. (ucpress.edu)
The inventory of amino acid changes in H5N1 influenza viruses helps researchers identify amino acid sequences that may increase pandemic potential. (cdc.gov)
A group of influenza subject matter experts compiled the H5N1 Inventory of amino acid changes in viruses' genetic sequence or amino acid motifs (varying combinations of amino acid sequences) by: (1) conducting a literature review of published and publically available information on H5N1 genetic changes, and (2) using a text mining process that examined existing PubMed H5N1 publications for information about changes in the genetic makeup of H5N1 viruses. (cdc.gov)
Contiguous amino acid sequences within a carbohydrate-active enzyme with discrete folds having carbohydrate binding activity. (bvsalud.org)
MRNA2
Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)
Homo sapiens chemokine (C-X-C motif) receptor 4 (CXCR4), mRNA. (lu.se)
Ligand2
Chemokine (C-X-C motif) ligand 9 (CXCL9), also commonly known as monokine induced by interferon-gamma (MIG), is a small cytokine belonging to the CXC chemokine family and is closely related to two other ELR-negative CXC chemokines: CXCL10 and CXCL11. (immunochemistry.com)
The human GR is a modular protein composed of distinct regions illustrated in panel B in the image below, as follows: (1) The amino-terminal A/B region, also called immunogenic or N-terminal domain (NTD) and (2) the C, D, and E regions, which correspond to the DNA-binding domain, the hinge region, and the ligand-binding domain, respectively. (medscape.com)
Homology1
It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. (nih.gov)
Analyzed amino acid1
7] analyzed amino acid variations of 15 different PTMs and indicated that about 4.5% of amino acid variations may affect protein function through disruption of PTMs, and the mutation of 238 PTMs sites in human proteins was causative of disease. (deepdyve.com)
Recognition7
The largest group of single strand RNA-binding protein is the eukaryotic RNA recognition motif (RRM) family that contains an eight amino acid RNP-1 consensus sequence. (wikipedia.org)
Most PTMs are catalyzed by highly specific protein modifying enzymes, which have some specific recognition motif. (deepdyve.com)
As this motif is different in the two CBR subtypes, we mutated lysine 402 of CB(1)R into glycine, to obtain a cholesterol recognition/interaction amino acid sequence and consensus similar to that of CB(2)R. Both mutated and wild-type receptors were transiently expressed in human neuronal SH-SY5Y cells, and their localization and functioning were investigated using biochemical assays and immunofluorescence labelling. (unboundmedicine.com)
Furthermore, this cleavage rate was improved by removing a competing protease recognition motif from the fusion partner. (edu.au)
Subunit1
Together, these findings suggest that agrin-induced phosphorylation of the β subunit motif increases the stoichiometry of rapsyn binding to the AChR, thereby helping to stably cluster the receptor and anchor it at high density in the postsynaptic membrane. (jneurosci.org)
A critical structural difference between class I and II HCPs is the N-terminal Cys-rich motif. (nature.com)
Those structural motifs are well known to play a key role in stabilizing protein structure and likely to be important in the protein folding process. (nih.gov)
The MICS program is developed based on a systematic study of the NMR chemical shift (and amino acid sequence) patterns observed for each type of structural motif by using a database of proteins of known structure and known NMR chemical shifts. (nih.gov)
Performs the secondary structure and structural motif classifications, then summarizes the predictions. (nih.gov)
Phosphorylation2
The amino acid variation S326C of human OGG1 disrupts Ser-326 phosphorylation site and affects susceptibility to a variety of cancers [9]. (deepdyve.com)
Following the evaluation of the model and the identification of motifs characteristic of the Flaviviridae family, data from previous publications on the treatment of HCV were incorporated, with the aim of detecting the ideal residues in the Spondweni model, which are similar to those of the HCV structure and are inhibitor targets. (spandidos-publications.com)
Glycine2
The glycine receptor , or GlyR , is the receptor for the amino acid neurotransmitter glycine . (bionity.com)
For instance, the Type I protein arginine methyltransferases are known to methylate a number of proteins that contain an arginineglycine glycine (RGG)-motif [6]. (deepdyve.com)
Gene1
The armadillo (Arm) repeat is an approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila melanogaster segment polarity gene armadillo involved in signal transduction through wingless. (embl.de)
Helix3
one binds in a groove at the barbed end of the actin monomer in a manner closely resembling the helix of WH2 domains, a motif found in many actin nucleation factors. (nih.gov)
These genes comprise 289 Transcription Factors (TFs) representing Basic Helix-loop Helix (bHLH), Ethylene Response Factors (ERFs), myeloblastosis (MYB), No apical meristem (NAC), and WRKY amino acid motif (WRKY) type major families known to be involved in the mechanism of stress tolerance. (frontiersin.org)
The chemical shifts, together with the PDB-extracted amino acid preference of the helix capping and beta-turn motifs, are then used as input data for training an artificial neural network algorithm, which outputs the statistical probability of finding each motif at any given position in the protein. (nih.gov)
Fragment1
The results showed that the amplified fragment between two motifs from V. dahliae with the reference protein has 66.3% similarities at the amino acid level. (ac.ir)
Proteomic analysis and prediction of amino acid variations that influence protein. (deepdyve.com)
To increase the utilization of current computational resources, we rst provide an overview of computational prediction of amino acid variations that influence protein PTMs and their functional analysis. (deepdyve.com)
During the prediction, MICS will generate a single output file ' predMICS.tab ' to store the the normalized probability of finding each motif at any given position in the protein, respectively. (nih.gov)
Specificity1
They are classified into 43 different families based on AMINO ACID SEQUENCE, binding specificity, and structure. (bvsalud.org)
Arginine1
There have been 17 different C-X-C chemokines described in mammals that are subdivided into two categories: those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the C-X-C motif (ELR-positive), and those without an ELR motif (ELR-negative). (immunochemistry.com)
Mature human IFNGR1 consists of an extracellular domain (ECD) with 2 Ig-like domains, a transmembrane domain and an intracellular domain with both Jak1 and Stat1 binding motifs. (rndsystems.com)
Two transmembrane domains were predicted in p11.7, and a DNA binding motif was found in p31.7 by amino acid sequence analysis, therefore p11.7 and p31.7 were predicted to be envelope and capsid protein, respectively. (umass.edu)
CXCR21
IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. (thermofisher.com)
Deletions1
By making deletions in the β loop we defined a 20 amino-acid sequence that is sufficient for clustering. (jneurosci.org)
Secondary1
The MICS output assigns a normalized probability for each residue to participate in any of the specific motifs, or to be part of a regular element of secondary structure. (nih.gov)
PTMs are specific to types of amino acid residues. (deepdyve.com)
Domain3
a Domain organization of the four subunits of human Pol δ and amino acid sequence of PCNA-interacting (PIP-box) motifs. (nature.com)
The extracellular domain of mouse Integrin beta 6 contains a collagen binding von-Willebrand factor A domain and multiple truncated EGF-like motifs. (rndsystems.com)
Mouse Integrin beta 6 extracellular domain shares 96% and 91% amino acid sequence identity with the corresponding regions of the rat and human protein, respectively. (rndsystems.com)
These data suggested that homologues transcripts encoded to WD40 repeat-contaning protein can be isolated from V. dahliae using degenerate primers from the common motif regions through a local allignment search method. (ac.ir)
Highly1
The viral genome sequence is highly divergent, and the encoded proteins have an average of only 55% amino acid sequence identity to those of other adenoviruses. (bvsalud.org)
Form1
Some sections of proteins form standard "motifs": corkscrew-like coils called alpha helices and flat sections called beta sheets. (nih.gov)