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  • peptides
  • Taking advantage of reliable metabolic labeling and accurate isobaric MS2 quantification, we developed a global in vivo terminal amino acid labeling (G-IVTAL) strategy by combining metabolic labeling and isotopic dimethyl labeling for quantifying tryptic peptides. (rsc.org)
  • tRNA
  • In this technique, truncated tRNAs are enzymatically ligated to chemically aminoacylated nucleotides, effectively decoupling the identity of the tRNA from that of the attached amino acid. (openwetware.org)
  • alanine
  • Here we initially reported a novel kind of gadofullerene nanoparticles functionalized with amino acid (β-alanine), which exhibited a superior antitumor activity in hepatoma H22 models via a novel therapeutic mechanism. (sigmaaldrich.com)
  • Stable
  • Stable isotope enrichments and amino acid levels were determined by gas chromatography-mass spectrometry in both plasma and whole blood and estimated (using hematocrit) in erythrocytes. (semanticscholar.org)
  • The methodology involves a continuous infusion of stable isotope tracer of essential amino acid and the three compartment model established in the leg muscle for the measurement of transmembrane amino acid transport of skeletal muscle and the rate of appearance of amino acids of both the whole body and of the skeletal muscle cells. (nii.ac.jp)
  • substrate
  • We used the Tc PAM structure in complex with ( E )-cinnamate, which functions as both a substrate and an intermediate, and the Pa PAM structure to account for the distinct β-amino acid stereochemistries. (msu.edu)
  • synthesis
  • In adrenalectomized rats receiving no glucocorticoids there was no statistical difference in amino acid oxidation, protein degradation or synthesis whether or not the rats had acidosis. (ndsl.kr)
  • Marked increase in transmembrane amino acid transport was observed after exercise, which may be an important mechanism of the increase in protein synthesis in skeletal muscle typically seen after exercise and/or the repeated muscle training. (nii.ac.jp)
  • Additional long-term continuous infusion of high-dose of insulin along with high-carbohydrate enteral feeding markedly improved protein balance of the skeletal muscle, protein synthesis and transmembrane amino acid transport. (nii.ac.jp)
  • These results may indicate that impaired transmembrane amino acid transport that provides the intracellular free amino acids used for protein synthesis is one of the important mechanism responsible for the increase in net protein loss causing negative protein balance in severely-burned patients and that the administration of anabolic hormone could overcome the impaired amino acid transport, resulting in the prevention of protein loss seen in critically ill patients. (nii.ac.jp)
  • tyrosine
  • Tyrosine-free amino acid mixture attenuates amphetamine-induced displacement of [11C]raclopride in striatum in vivo: a rat PET study. (ox.ac.uk)
  • Previous neurochemical and behavioural studies show that tyrosine depletion using a nutritionally balanced tyrosine-free amino acid mixture attenuates the dopamine-releasing and psychostimulant properties of amphetamine. (ox.ac.uk)
  • Here we investigate the effect of a tyrosine-free amino acid mixture on striatal binding of [(11)C]raclopride, and amphetamine-induced [(11)C]raclopride displacement, using positron emission tomography in the rat. (ox.ac.uk)
  • The tyrosine-free amino acid mixture (1 g/kg i.p.) caused a small (+7%) but statistically insignificant increase in [(11)C]raclopride DVR and attenuated, although it did not fully block, the amphetamine-induced reduction. (ox.ac.uk)
  • These data are in keeping with previous neurochemical, immunocytochemical, and behavioural studies showing that tyrosine-free amino acid mixtures reduce dopamine function and offer promise for future PET studies testing the effect of tyrosine-depleting paradigms on dopamine release in humans. (ox.ac.uk)
  • bacterial
  • In this report, we exploited one key difference, bacterial cell wall biosynthesis, to detect living bacteria using a positron-labeled D-amino acid. (physiciansweekly.com)
  • We believe that the ease of [(11)C] D-Met radiosynthesis, coupled with its rapid and specific in vivo bacterial accumulation, make it an attractive radiotracer for infection imaging in clinical practice. (physiciansweekly.com)
  • genes
  • Interestingly, the acid-stressed genes, hdeB and hdeD , were downregulated, and such downregulation partly accounted for the decrease in tolerance of the tyrR mutant under acidic conditions. (frontiersin.org)
  • Transfer of the genes encoding these enzymes into a chassis organism can potentially make various bioactive molecules in vivo or in vitro . (msu.edu)
  • metabolic
  • We conclude that metabolic acidosis accelerates amino acid oxidation and protein degradation in vivo, and that glucocorticoids are necessary but not sufficient to mediate the catabolic effects of metabolic acidosis. (ndsl.kr)
  • cells
  • Cells may be grown in presence of the canonical amino acid, and then removed from the growth medium and inoculated into growth medium containing none of the canonical amino acid, but an overabundance of a close structural analog. (openwetware.org)
  • Studies
  • Bioequivalence methodologies for topical drug products:in vitro and ex vivo studies with a cortiscosteroid and an anti-fungal drug. (bath.ac.uk)
  • specific
  • In order to visualise both Poly-, and Mono-, ADP-ribosylation in vivo, we engineered specific fluorescent probes. (uu.nl)
  • transport
  • Alterations of amino acid and protein kinetics and transmembrane amino acid transport of skeletal muscle seen in physiological circumstance and in severely-burned patients were reviewed. (nii.ac.jp)
  • In severely burned patients, on the other hand, transmembrane amino acid transport of skeletal muscle was markedly impaired and the long-term continuous high-carbohydrate enteral feeding providing enough calorie improved neither amino acid and protein balance in the leg nor amino acid transport. (nii.ac.jp)
  • No further improvement of amino acid kinetics and amino acid transport was observed by, the additional administration of growth hormone. (nii.ac.jp)
  • Publications] Yoichi Sakurai: 'Effects of long-term high carbohydrate enteral feeding on trans mew brane amino acid transport in severely-burned palieuts' Surgical Forum. (nii.ac.jp)
  • systems
  • The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris. (osti.gov)
  • necessary
  • We show that dPARP16 catalytic activity is necessary and sufficient for both amino-acid starvation induced mono-ADP-ribosylation and subsequent Sec body formation and cell survival. (uu.nl)
  • common
  • Furthermore, we characterize the photophysical properties of Acd, including quenching interactions with select natural amino acids and Förster resonance energy transfer (FRET) interactions with common fluorophores such as methoxycoumarin (Mcm). (oregonstate.edu)
  • increase
  • In contrast, chronically acidotic, adrenalectomized rats receiving glucocorticoids demonstrated accelerated whole body protein turnover with a 84% increase in amino acid oxidation and a 26% increase in protein degradation, compared to rats not receiving glucocorticoids or those given the same dose of glucocorticoids but without acidosis. (ndsl.kr)
  • levels
  • Effect of three different intensities of infrared laser energy on the levels of amino acid neurotransmitters in the cortex and hippocampus of rat brain. (semanticscholar.org)