Amino Acid SequenceMolecular Sequence DataModels, MolecularProtein BindingBinding SitesProtein ConformationProtein Structure, TertiaryAmino AcidsSequence Homology, Amino AcidMutagenesis, Site-DirectedAmino Acid SubstitutionBase SequenceProtein Interaction MappingProtein Structure, SecondaryTranscriptional ActivationHydrophobic and Hydrophilic InteractionsTwo-Hybrid System TechniquesMutationStructure-Activity RelationshipSequence AlignmentTranscription FactorsCell LineProteinsCrystallography, X-RayKineticsCloning, MolecularHydrogen BondingAmino Acid MotifsRecombinant ProteinsProtein FoldingEscherichia coliSequence Analysis, ProteinSubstrate SpecificityPeptidesPeptide FragmentsRecombinant Fusion ProteinsCatalytic DomainDNA-Binding ProteinsCatalysisThermodynamicsConserved SequenceStatic ElectricityDatabases, ProteinTransfectionDNACircular DichroismMutagenesisAlanineCysteineModels, ChemicalLeucineBacterial ProteinsSolventsCOS CellsLysinePlasmidsDNA, ComplementaryTranscription, GeneticMolecular StructureComputer SimulationDimerizationPoint MutationMagnetic Resonance SpectroscopyTrans-ActivatorsPromoter Regions, GeneticDNA PrimersPhosphorylationSequence DeletionIsoleucineTryptophanSequence Homology, Nucleic AcidPhenylalanineElectrophoresis, Polyacrylamide GelAmino Acids, AromaticCarrier ProteinsNuclear ProteinsHistidineSerineValineCattleMolecular WeightHeLa CellsAmino Acids, EssentialMembrane ProteinsSaccharomyces cerevisiaeArginineLigandsSoftwareNuclear Magnetic Resonance, BiomolecularAspartic AcidTrypsinAmino Acid Transport SystemsHydrogen-Ion ConcentrationCercopithecus aethiopsRestriction MappingComputational BiologyChromatography, High Pressure LiquidProtein Processing, Post-TranslationalTyrosineHydrolysis