Amino acid sequences amino acid substitution. Medical search. Frequent questions

In bioinformatics, the BLOSUM (BLOcks SUbstitution Matrix) matrix is a substitution matrix used for sequence alignment of proteins. (wikipedia.org)
In order to fill in this gap, Henikoff and Henikoff introduced BLOSUM (BLOcks SUbstitution Matrix) matrix which led to marked improvements in alignments and in searches using queries from each of the groups of related proteins. (wikipedia.org)
The predicted structures of the Gimap proteins show common sequences and motifs, such as GTP-binding domains in the N-terminal half, but with differing C-terminal ends [ 2 , 3 ]. (hindawi.com)
The fact that the Gimap genes are located together in a tight cluster on RNO4 (and in conserved synteny with many other species), combined with their sequence similarities, suggests the possibility that the proteins carry out similar function. (hindawi.com)
Additionally, the investigation of nucleotide sequences that code for important proteins, such as phaseolin and iron-reductase, encoded respectively by the Phs and FRO1 genes, should allow new and informative markers to be generated, helping us to gain a better understanding of intraspecific variation in P. vulgaris. (usp.br)
the alphabet is the 20 proteinogenic amino acids for proteins and the sense codons (i.e., the 61 codons that encode amino acids in the standard genetic code ) for aligned protein-coding gene sequences. (wikipedia.org)
In human clinical trials, proteins from two companies, each containing an unnatural amino acid, appear to hold promise as longer-lasting treatments for multiple sclerosis (MS) and growth hormone deficiency. (acs.org)
However, "the native 20 amino acids that comprise proteins limit one's ability to do precise chemical modifications," says Ho Sung Cho, chief technology officer of - Ambrx , a La Jolla, Calif.-based biotechnology company specializing in developing drugs containing unnatural amino acids. (acs.org)
His team's most advanced technique for inserting unnatural amino acids into proteins borrows two pieces of the protein translation apparatus from a single-celled microorganism called Methanococcus jannaschii -a transfer RNA and an enzyme called an aminoacyl tRNA synthetase that loads that tRNA with an amino acid. (acs.org)
Importantly, the amino acid diversity of EMAs is distributed throughout the length of the proteins. (biomedcentral.com)
Amino acid substitution scoring matrices specific to intrinsically disordered regions in proteins. (bvsalud.org)
An amino acid substitution scoring matrix encapsulates the rates at which various amino acid residues in proteins are substituted by other amino acid residues, over time . (bvsalud.org)
However, widely used substitution scoring matrices, such as BLOSUM series, have been developed using aligned blocks that are mostly devoid of disordered regions in proteins . (bvsalud.org)
Hence, these substitution-scoring matrices are mostly inappropriate for homology searches involving proteins enriched with disordered regions as the disordered regions have distinct amino acid compositional bias , and therefore expected to have undergone amino acid substitutions that are distinct from those in the ordered regions. (bvsalud.org)
We, therefore, developed a novel series of substitution scoring matrices referred to as EDSSMat by exclusively considering the substitution frequencies of amino acids in the disordered regions of the eukaryotic proteins . (bvsalud.org)

Our aim was to clone and sequence the cDNA of the BB diabetes prone (DP) and diabetes resistant (DR) alleles of all seven Gimap genes in the congenic DR. lyp rat line with 2 Mb of BB DP DNA introgressed onto the DR genetic background. (hindawi.com)
Therefore, the aims of this study were to sequence the -Phs and FRO1 genes, to assess SNP frequencies in coding and non-coding regions of both genes, and to assess the possible use of these polymorphisms for investigating the genetic diversity of 31 cultivated genotypes of common bean and one of P. lunatus. (usp.br)
As a result, we further demonstrate the acquisition of novel human sequence, including novel genes not previously identified by traditional genomic sequencing. (columbia.edu)
In a comparison excluding the composite sequencing data, the genomes and the open reading frames are fairly well conserved with differences only in 2, out of 80, genes. (usda.gov)
Comparison of the genome sequences of phenotypically different strains could help to identify genes involved in virulence. (usda.gov)
Sequence analysis revealed two variant CYP2D6 genes, CYP2D6Ch1 and CYP2D6Ch2, having mutations yielding two and eight amino acid substitutions, respectively. (aspetjournals.org)
Comparison of non-synonymous (amino acid altering) substitution rates with synonymous substitution rates of D. galeata mt protein-coding genes demonstrated that ATPase8 and COI genes were the most and least susceptible, respectively, to the evolutional forces selecting the aa substitutions. (unboundmedicine.com)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
Two nucleotides were different between the E. coli (Tn3) and H. ducreyi (pCb) genes that affected the amino-acid sequence. (lu.se)
This project aims to estimate the ratio of non-synonymous to synonymous substitutions (dN/dS) utilizing the PAML framework in an effort to unravel the selective pressures sculpting the molecular landscape of ubiquinone biosynthesis genes in sponges, contributing to a deeper understanding of their evolutionary strategies. (lu.se)
Neutral evolution is anticipated when genes become redundant, while purifying selection safeguards essential amino acid sequences. (lu.se)

BLOSUM matrices are used to score alignments between evolutionarily divergent protein sequences. (wikipedia.org)
BLOSUM matrices with high numbers are designed for comparing closely related sequences, while those with low numbers are designed for comparing distant related sequences. (wikipedia.org)
We calculated p16 evolution using amino acid substitution matrices and nucleotide substitution distances. (nih.gov)
We have been optimising amino acid substitution matrices for protein sequence alignments. (uni-hamburg.de)
Database search methods make use of substitution scoring matrices to identify sequences with homologous relationships. (bvsalud.org)
Areas that will be covered include: sequence databases, pairwise and multiple sequence alignment, searches in sequence databases, amino acid substitution matrices, secondary structure, prediction of RNA and polypeptides, and models for protein classification. (lu.se)

METHODS: We have used a combination of a multiple sequence alignment of orthologous BRCA1 sequences and a measure of the chemical difference between the amino acids present at individual residues in the sequence alignment to classify missense variants and in-frame deletions detected during mutation screening of BRCA1 . (xenbase.org)
The amino acid residues Asp30, Glu32, Asp112 and Asp251 are the 3'-5' exonuclease active sites of IME199 DNAP, while residues Asp596 and Tyr639 are essential for DNA synthesis by IME199 DNAP. (bvsalud.org)
Partial Order Optimum Likelihood (POOL), a machine learning method that uses input features from Theoretical Microscopic Titration Curve Shapes (THEMATICS), was used to identify amino acid residues most likely involved in catalytic activity. (bvsalud.org)

Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. (jneurosci.org)
Sequence analysis of BRCA1 and BRCA2: correlation of mutations with family history and ovarian cancer risk. (xenbase.org)
In nearly all patients with Apert syndrome, the cause is 1 of 2 FGFR2 mutations involving amino acids (Ser252Trp, Pro253Arg). (medscape.com)
Both AU and GN gene sequences contained other similar silent and missense mutations. (weedscience.org)
Background De novo mutations are emerging as an important cause of neurocognitive impairment, and whole exome sequencing of case-parent trios is a powerful way of detecting them. (bmj.com)
This experiment was conducted to study hybridization between CL rice and red rice using simple sequence repeats (SSR) markers, identify mutations in the ALS gene of imazethapyr-resistant red rice, and to detect the introgression of the ALS-resistant gene from CL rice into red rice. (weedscience.com)
Overall our comparative sequence analysis of virulent GaHV-1 strains has identified both intragenic and intergenic deletions as well as single nucleotide polymorphism mutations that differentiate the Serva vaccine genome from the genomes of 4 virulent strains. (usda.gov)
Complete genomic sequences of 12 different coronavirus strains demonstrated that the viral structure, with small irrelevant mutations, was stable in the transmission chains and showed remarkable traits of infectious traceability. (ersjournals.com)

This report describes SARS-CoV-2 genomic surveillance conducted by the Department of Defense (DoD) Global Emerging Infections Surveillance Branch and the Next-Generation Sequencing and Bioinformatics Consortium (NGSBC) in response to the COVID-19 pandemic. (health.mil)
With recent availability of next-generation sequencing (NGS), it is becoming more common to pursue disease-targeted panel testing rather than traditional sequential gene-by-gene dideoxy sequencing. (molvis.org)

Arbitrary genome sequences as well as genic sequences were used for this purpose. (usp.br)
S-Y. Chang and colleagues report in Poultry Science that they performed a multiple sequence alignment and phylogenetic analyses on the 24 viral genome sequences as well as on 45 genome sequences available from the GenBank database. (thepoultrysite.com)

The K a /K s ratio can be used to examine the action of natural selection on protein-coding regions, [5] [6] it provides information about the relative rates of nucleotide substitutions that change amino acids (non-synonymous substitutions) to those that do not change the encoded amino acid (synonymous substitutions). (wikipedia.org)
Several non-synonymous substitutions were found in ATPase8 and ATPase6 even in the comparison that no synonymous substitution was found. (unboundmedicine.com)

4-6 The first sequenced and publicly released SARS-CoV-2 genomes helped guide development of diagnostic assays and eventually vaccines. (health.mil)
However, the formidable size of the diploid human genome, approximately 6 gigabases, has prevented the routine application of sequencing methods to deciphering complete individual human genomes. (columbia.edu)
Only two ORFs, ICP4 and the US2 differed in amino acid (a.a.) lengths among the newly sequenced genomes. (usda.gov)
78 synonymous and 118 non-synonymous amino acid substitutions were identified in a comparison that included ORFs encoded within the genomes of the Serva and group I, III, V and VI strains. (usda.gov)

Scores for each position are obtained frequencies of substitutions in blocks of local alignments of protein sequences. (wikipedia.org)
Amino acid substitution during functionally constrained divergent evolution of protein sequences. (xenbase.org)

Phylogenetic analysis of Bma-RXR and related nuclear receptor sequences. (figshare.com)
Substitution models are used to calculate the likelihood of phylogenetic trees using multiple sequence alignment data. (wikipedia.org)
Substitution models are also central to phylogenetic invariants because they are necessary to predict site pattern frequencies given a tree topology. (wikipedia.org)
These site patterns are used to calculate the likelihood given the substitution model and a phylogenetic tree (in this case an unrooted four-taxon tree). (wikipedia.org)
Phylogenetic analyses based on the genomic and open reading frame 2/cap sequences demonstrated that all DuCV strains belonged to genotype 1 and were designated in a sub-cluster under genotype 1. (thepoultrysite.com)

Multiple sequence alignment (in this case DNA sequences) and illustrations of the use of substitution models to make evolutionary inferences. (wikipedia.org)

In sickle cell anemia, a point mutation on the β-globin gene results in glutamic acid substituting for valine at position 6 of the amino acid sequence. (medscape.com)

A) causes a substitution of a highly conserved amino acid Arginine to a Glutamine residue at position 41 (p.Arg41Gln). (academicjournals.org)
Ser amino acid substitution in a region that is a highly conserved in cytochromes P450 belonging to gene families 1 and 2. (aspetjournals.org)

However, in addition to being faster and significantly less expensive, this sequencing technology avoids the arbitrary loss of genomic sequences inherent in random shotgun sequencing by bacterial cloning because it amplifies DNA in a cell-free system. (columbia.edu)

Nucleotide sequences of the ALS gene from the rice cultivars were identical. (weedscience.com)
The nucleotide sequences of four virulent strains of gallid herpesvirus type 1, the causative agent of infectious laryngotracheitis, were determined. (usda.gov)
Sanger dideoxy sequencing, 454 pyrosequencing and Illumina sequencing were used to determine the nucleotide sequences of four groups of virulent GaHV-1 strains (groups I, III, V and VI). (usda.gov)

They scanned the BLOCKS database for very conserved regions of protein families (that do not have gaps in the sequence alignment) and then counted the relative frequencies of amino acids and their substitution probabilities. (wikipedia.org)
Sequence alignment is a fundamental research method for modern biology. (wikipedia.org)
The most common sequence alignment for protein is to look for similarity between different sequences in order to infer function or establish evolutionary relationships. (wikipedia.org)
Scoring metrics (statistical versus biological): When evaluating a sequence alignment, one would like to know how meaningful it is. (wikipedia.org)
This requires a scoring matrix, or a table of values that describes the probability of a biologically meaningful amino-acid or nucleotide residue-pair occurring in an alignment. (wikipedia.org)
Partial amino acid sequence alignment of keratoepithelin with ten of its orthologs. (molvis.org)
The majority of substitution models used for evolutionary research assume independence among sites (i.e., the probability of observing any specific site pattern is identical regardless of where the site pattern is in the sequence alignment). (wikipedia.org)

0.05, the evolutionary substitution database must contain at least 3(M) variants, where M equals the number of codons in the gene. (nih.gov)
These data validate our hypothesis that detailed evolutionary analyses help predict the consequences of missense amino-acid variants. (nih.gov)
As demonstrated by the emergence of multiple SARS-CoV-2 variants, sequencing is needed to monitor viral evolution and inform mitigation strategies in DoD settings, including troop movement and hygiene measures. (health.mil)
Two sequence variants were identified in each of the four genomic segments that harbor hlyC, cbiQ-glyA, trxA-truB-rsuA, and rplS-tyrS-csdB, respectivly. (usda.gov)

Sanger sequencing of the entire coding region and the intron-exon junctions of the HMGCL gene identified a recurrent missense mutation in exon 2. (academicjournals.org)
Initial genetic analysis was performed on one family member with traditional Sanger single gene sequencing and/or panel-based testing, and ultimately, retinal gene-targeted NGS was required to identify the underlying cause of disease for individuals within the three families. (molvis.org)

Genome sequence alignments were used to identify two regions (5' terminus and the unique short/repeat junction) that contained various intragenic deletions. (usda.gov)

The current p16 evolutionary substitution database is too small to determine whether observations of 'absolute conservation' are statistically significant. (nih.gov)
Increasing the number of sequences from three to seven significantly improved the predictive value of evolutionary computations. (nih.gov)
In biology, a substitution model , also called models of DNA sequence evolution , are Markov models that describe changes over evolutionary time. (wikipedia.org)
Estimates of evolutionary distances (numbers of substitutions that have occurred since a pair of sequences diverged from a common ancestor) are typically calculated using substitution models (evolutionary distances are used input for distance methods such as neighbor joining ). (wikipedia.org)
It is also necessary to assume a substitution model to estimate evolutionary distances for pairs of sequences (distances are the number of substitutions that have occurred since sequences had a common ancestor). (wikipedia.org)
the four site patterns that differ between taxa 1 and 2 are indicated with asterisks) into an evolutionary distance (in this case d 12 =0.2635 substitutions per site). (wikipedia.org)
The method is trained by using severe and mild/moderate (called as non-severe) disease-causing variations and uses evolutionary conservation, properties of amino acids and sequence environment for prediction. (lu.se)

The exception is the final exon which not only codes the terminal portion of the receptor, but also includes a 2,147 bp non-coding sequence that follows. (creation.com)
Exon 9 of the CYP2D6Ch2 gene contained a sequence of 49 bases originating from the pseudogene CYP2D7P. (aspetjournals.org)

To address the gap of knowledge on this, the complete sequences of eight plasmids from Pandoraea spp. (frontiersin.org)

Alternative splicing generates an additional isoform with a substitution of the N-terminal 10 amino acids including the signal peptide. (rndsystems.com)
Redwood Bioscience builds libraries of peptide conjugates with unnatural amino acids. (acs.org)
They've developed technologies to introduce unnatural amino acids into a peptide sequence. (acs.org)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)

Since the beginning of the pandemic, viral sequencing has been a critical component of the response. (health.mil)
The demographics and clinical features of the 35 laboratory-confirmed cases were investigated and viral RNA of 12 cases was sequenced and analysed. (ersjournals.com)

BLOSUM62 is the matrix built using sequences with less than 62% similarity (sequences with ≥ 62% identity were clustered) - Note: BLOSUM 62 is the default matrix for protein BLAST. (wikipedia.org)
Two widely distributed lepidopteran herbivores, codling moth Cydia pomonella (Tortricidae) feeding in apples and pears, and the African cotton leafworm Spodoptera littoralis (Noctuidae), a moth feeding on foliage of a wide range of herbaceous plants, both express a receptor ortholog, OR19, which shares 58% amino acid identity and 69% amino acid similarity. (frontiersin.org)

Analysis of missense variation in human BRCA1 in the context of interspecific sequence variation. (xenbase.org)
Predicting the functional consequences of non-synonymous single nucleotide polymorphisms: structure-based assessment of amino acid variation. (xenbase.org)
PON-PS requires users to submit fasta-format amino acid sequence(s) and variation(s) in the corresponding sequence(s). (lu.se)
The variation(s) corresponding to a sequence should contain the same header line as the sequence. (lu.se)
The sequence(s) and variation(s) can be pasted in the correponding text-boxes or separate files containing sequence(s) and variation(s) can be submitted. (lu.se)

Together, these results indicate that the structure of the rhodopsin N terminus must be maintained by an appropriate amino acid sequence surrounding N2 and may require a carbohydrate moiety at N15. (jneurosci.org)

The K a /K s ratio (also called ω in codon substitution models) is a parameter of interest in many studies. (wikipedia.org)

4 We screened the coding sequence of ESPN (GenBank accession number AL136880) by sequencing PCR amplified ESPN exons from genomic DNA of affected individuals in the two families. (bmj.com)

The 20 amino acids translated by the genetic code vary greatly by the physical and chemical properties of their side chains. (wikipedia.org)
Only a few amino acids have reactive side chains, and unless only one copy of that reactive amino acid is present in a protein of interest, chemical modifications tend to result in heterogeneous protein mixtures, Cho says. (acs.org)

Case 4 was a phenotypically isolated case recruited into an undiagnosed rare disorders sequencing study. (bmj.com)

Changing a single amino acid in a protein may reduce its ability to carry out this function, or the mutation may even change the function that the protein carries out. (wikipedia.org)
The human coding sequence of MYH16 has a frameshift mutation that prevents translation of the functional product. (johnhawks.net)
Sequencing of the ALS gene revealed a point mutation resulting in an amino acid substitution at Trp 574 . (weedscience.org)
Moreover, to determine the protein stability upon change in amino acid various tools including SDM, I-Mutant, mCSM and DUET were used and found that the mutation, identified in this family, is protein destabilizing. (academicjournals.org)

Characterization of disease-associated single amino acid polymorphisms in terms of sequence and structure properties. (xenbase.org)
This single amino acid substitution results in the formation of sickle cell hemoglobin. (medscape.com)
Here we report the DNA sequence of a diploid genome of a single individual, James D. Watson, sequenced to 7.4-fold redundancy in two months using massively parallel sequencing in picolitre-size reaction vessels. (columbia.edu)
Comparison of the sequence to the reference genome led to the identification of 3.3 million single nucleotide polymorphisms, of which 10,654 cause amino-acid substitution within the coding sequence. (columbia.edu)
Overall, these results agree well with recent results of sequencing of a single individual by traditional methods. (columbia.edu)
A single amino acid substitution from Tryptophan 574 to Leucine has led to resistance to Inhibition of Acetolactate Synthase as indicated in the table below. (weedscience.org)

We collected draft genome sequence data and associated metadata data on 76 N. gonorrhoeae strains from around the globe and searched for known determinants of antibiotics resistance within the strains. (peerj.com)
Analysis of the capsid protein amino acid sequences of the 24 Korean DuCV strains showed 10 substitutions compared with that of other genotype 1 strains. (thepoultrysite.com)

To characterize genetic diversity and gene flow among Daphnia galeata populations, the complete nucleotide (nt) sequences of the mitochondrial (mt) DNAs of D. galeata clones isolated from five lakes in Japan (Lakes Shirakaba, Suwa, Kizaki, Kasumigaura, and Biwa) were determined. (unboundmedicine.com)
High-throughput sequencing of resistant clones and reference HSV-1/L2 parent strain was performed to identify the genetic determinants of the virus's resistance to the lead compound. (bvsalud.org)

Analysis of 2179 clonal sequences derived from hypervariable region 1 (HVR1) of the HCV genome in samples obtained from patients with acute (n = 49) and chronic (n = 102) HCV infection showed that intra-host HVR1 diversity was 1.8 times higher in patients with chronic than acute infection. (iospress.com)
Clinical and molecular genetic analysis of a Saudi family with five individuals affected with HMGCLD was performed by GC-MS, tandem MS and sequencing. (academicjournals.org)
Analysis of the sequencing data revealed numerous discrepancies with the published sequence of a composite genome. (usda.gov)
Sequence analysis of the haemagglutinin gene was performed in 64 selected influenza B isolates. (who.int)
The primary aim of the course is that the students shall acquire deeper understanding of, and skills in, basic concepts and tools for comparative sequence analysis, including various types of primary and secondary sequence databases. (lu.se)

To identify such signals we characterized conserved sequence motifs in the Serp deacetylase domain. (avhandlingar.se)

[3] thus, branch lengths and any other parameters describing the substitution process are often viewed as nuisance parameters . (wikipedia.org)

In this study, we identified a transcript variant of EZH2 in porcine fetal tissues by cloning and sequencing. (hindawi.com)

Then, they calculated a log-odds score for each of the 210 possible substitution pairs of the 20 standard amino acids. (wikipedia.org)
They looked at more than 1100 base pairs of sequence from a chimpanzee, orangutan, macaque, and a dog, and they found that the coding sequence in humans has a small number of unexpected amino-acid-coding changes-changes that wouldn't be likely if the gene product was still being maintained by selection. (johnhawks.net)
Proximities between Amino Acids Deduced from Spontaneous Disulfide Bond Formation between Cysteine Pairs Engineered in Cytoplasmic Loops 1, 3 and 4. (cmu.edu)

The thiolate undergoes reversible (to sulfenic) or irreversible (to sulfinic and sulfonic acid) covalent modifications upon oxidation. (nature.com)
The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)
in December 2006, UniMod lists 495 modifications including 144 amino acid substitutions [6]. (lu.se)

From a series of 20,000 full sequence tests of BRCA1 carried out at Myriad Genetic Laboratories, a total of 314 different missense changes and eight in-frame deletions were observed. (xenbase.org)

Cloning of the ALS gene surrounding the Trp 574 region yielded two distinct ALS gene sequences: one producing Trp 574 and one producing Leu 574 . (weedscience.org)

Despite the small p16 sequence database, our calculations of high conservation correctly predicted loss of cell cycle arrest function in 75% of tested codons, and low conservation correctly predicted wild-type function in 80-90% of codons. (nih.gov)

The nuclear localization sequences of the BRCA1 protein interact with the importin-alpha subunit of the nuclear transport signal receptor. (xenbase.org)

Fifteen CaPsol lineages were identified based on didstinct combinations of sequence variations within these genetic loci. (usda.gov)
Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of one AGS-specific amino acid substitution in mediating cytoprotection by AGS ATP5G1. (elifesciences.org)

In fact, substitution models can be developed for any biological characters that can be encoded using a specific alphabet (e.g., amino acid sequences combined with information about the conformation of those amino acids in three-dimensional protein structures [7] [8] ). (wikipedia.org)

By comparing the sequences of humans and other primates, Stedman's team estimated that MYH16 had lost its function in human ancestors around 2.4 million years ago. (johnhawks.net)

Hansell Stedman and collaborators at the University of Pennsylvania had collected sequence data for a gene called MYH16 . (johnhawks.net)
Samples and sequence data were from SARS-CoV-2 infections occurring among Military Health System (MHS) beneficiaries from 1 March to 31 December 2020. (health.mil)
Sequence data were used to monitor transmission dynamics on U.S. Navy ships and at military training centers and installations. (health.mil)
[2] Substitution models are also necessary to simulate sequence data for a group of organisms related by a specific tree. (wikipedia.org)

Our web server is running and does threading using a mixture of sequence terms and a structural term based on Bayesian classification of fragment properties. (uni-hamburg.de)

Using a gene editing tool to selectively substitute some of the building blocks, also known as amino acids, that make up the ATP5G1 protein revealed that improvements to the mitochondria were caused by switching specific amino acids. (elifesciences.org)

Our results showed that the coding sequence of EZH2 was 2241 bp, encoding 746 amino acids. (hindawi.com)

This sequence was completed in two months at approximately one-hundredth of the cost of traditional capillary electrophoresis methods. (columbia.edu)
Methods The Deciphering Developmental Disorders study is using whole exome sequencing in family trios to investigate children with severe, sporadic, undiagnosed developmental delay. (bmj.com)

Anatomy9

Organisms16

Chemicals and Drugs98

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Phenomena and Processes92

Disciplines and Occupations2

Information Science4

Health Care2

Proteins15

Genes12

Matrices6

Residues3

Mutations8

Next-Generation Sequencing2

Genome sequences2

Synonymous substitutions2

Genomes4

Protein sequences2

Phylogenetic5

Multiple sequence1

Valine at position1

Highly conserved2

Genomic sequences1

Nucleotide sequences3

Alignment7

Variants4

Sanger2

Alignments1

Evolutionary7

Exon2

Complete sequences1

Peptide5

Viral2

Similarity2

Variation5

Rhodopsin1

Codon1

GenBank1

Side chains2

Phenotypically1

Mutation4

Single6

Strains2

Clones2

Analysis5

Motifs1

Lengths1

Variant1

Pairs3

Modifications3

Missense1

Distinct1

Conservation1

Nuclear1

Loci2

Biological1

Humans1

Data4

Properties1

Improvements1

Results1

METHODS2