• These results show that although the sequences of NA CT and TMD per se are not absolutely essential for the virus life cycle, specific amino acid sequences play a critical role in providing structural stability, enzyme activity, and lipid raft association of NA. (asm.org)
  • Together with American colleagues, the Mainz researchers have reported in the latest edition of the scientific journal Science Advances that the interactions of specific amino acid sequences of the protein molecules generate water domains with increased order and stronger hydrogen bonds. (mpg.de)
  • For many purposes the primary amino acid sequence of a protein can be directly used to predict important structural parameters. (caltech.edu)
  • In turn, each residue's contact number can be partially predicted from primary amino acid sequence, assisting tertiary fold analysis from sequence data. (biomedcentral.com)
  • In particular we study the effect of minor mutations of insulin's primary amino acid sequence on its interaction with 1,2-Dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) model lipid layers. (frontiersin.org)
  • In other aspects, replicable recombinant cloning vehicles are provided which code for plant 1-deoxyxylulose-5-phosphate synthases, or for a base sequence sufficiently complementary to at least a portion of 1-deoxyxylulose-5-phosphate synthase DNA or RNA to enable hybridization therewith. (osti.gov)
  • In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a plant 1-deoxyxylulose-5-phosphate synthase. (osti.gov)
  • 2. A recombinant expression vector comprising the nucleic acid of claim 1 operably linked to a transcription regulatory element. (patents.com)
  • Such a cleavage is essential for viral infectivity because in the acidic pH of the endosome it exposes a membrane fusion peptide at the amino terminus of the HA2 subunit required for membrane fusion ( 26 ). (asm.org)
  • HA cleavability was enhanced by a single amino acid substitution that abrogated glycosylation near the HA cleavage site. (cdc.gov)
  • In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof. (thefreedictionary.com)
  • Molecules with the varied properties required for these multifarious functions are built up of a mere 20 units: the protein-constituent amino acids, connected primarily in a single structural mode (a linear sequence) but finally arranged in space in intricate ways to molecules of the required size, shape and properties. (springer.com)
  • Allelic variation of the DRB1 DNA sequence can result in 3rd HVR amino acid sequences that differ for overall 3rd HVR charge and can impact the steric interaction between HLA molecules and T cells. (biomedcentral.com)
  • They are carbon compounds, made from an amine group (-NH 2 ), carboxylic acid group (-COOH), and a chain of carbon molecules of varying types. (biologywise.com)
  • Amino acid sequence of rabbit muscle aldolase and the structure of the active center. (uniprot.org)
  • This means that a change of a single amino acid has the potential to change the overall 3D shape of the entire protein, which could limit or remove its function(s). (gleekguide.com)
  • Single amino acid substitutions in influenza haemagglutinin change receptor binding specificity. (cdc.gov)
  • Their sequence is created through the bonding of the carboxyl group of one amino acid, with the amino group of another. (biologywise.com)
  • The sequence is generally reported from the N-terminal end containing free amino group to the C-terminal end containing free carboxyl group. (soscholar.com)
  • All proteinogenic amino acids possess common structural features, including an α-carbon to which an amino group, a carboxyl group, and a variable side chain are bonded . (wikipedia.org)
  • As a result, one of the most powerful approaches for gaining insight into genome sequences is based simply on amino acid sequence comparisons between new, uncharacterized amino acid sequences predicted from genome DNA sequences, and amino acid sequences of known function. (thefreedictionary.com)
  • Both of these tandem repeat sequences were identified at other locations in the chicken genome by Southern blot hybridization. (labome.org)
  • Although this bacterium is unable to degrade cellulose, the sequence analysis of its genome revealed the presence of a large cellulosomal gene cluster ( 12 ). (asm.org)
  • Comparative CD36 amino acid sequences and structures and CD36 gene locations were examined using data from several vertebrate genome projects. (mdpi.com)
  • A detailed survey involving unlabeling of different amino acid types individually or in pairs reveals that the proposed approach is also robust to misincorporation of N-14 at undesired sites. (iisc.ernet.in)
  • 1. The assumed specifically active fragment of bovine fibrinopeptide B with the amino acid sequence 15-21 and the fragment 12-21 containing the tripeptide residue Asp 12 -Arg 13 -Pro 14 have been synthesized. (springer.com)
  • We find that bovine and human insulin, even though very similar in sequence, show a substantially different behavior when interacting with lipid films. (frontiersin.org)
  • 37 CFR 1.821 Nucleotide and/or amino acid sequence disclosures in patent applications. (uspto.gov)
  • b) Patent applications which contain disclosures of nucleotide and/or amino acid sequences, in accordance with the definition in paragraph (a) of this section, shall, with regard to the manner in which the nucleotide and/or amino acid sequences are presented and described, conform exclusively to the requirements of §§ 1.821 through 1.825 . (uspto.gov)
  • 37 CFR 1.821(a) presents a definition for "nucleotide and/or amino acid sequences. (bitlaw.com)
  • The requirement for compliance in 37 CFR 1.821(c) is directed to "disclosures of nucleotide and/or amino acid sequences. (bitlaw.com)
  • All sequence information, whether claimed or not, that meets the length thresholds in 37 CFR 1.821(a) is subject to the rules. (bitlaw.com)
  • 1) If the "Sequence Listing" required by § 1.821(c) is submitted on paper: The "Sequence Listing," setting forth the nucleotide and/or amino acid sequence and associated information in accordance with paragraph (b) of this section, must begin on a new page and must be titled "Sequence Listing. (bitlaw.com)
  • 2) If the "Sequence Listing" required by § 1.821(c) is submitted on compact disc: The "Sequence Listing" must be submitted on a compact disc in compliance with § 1.52(e) . (bitlaw.com)
  • The presentation of the "Sequence Listing" and other materials on compact disc under § 1.821(c) does not substitute for the Computer Readable Form that must be submitted on disk, compact disc, or tape in accordance with § 1.824 . (bitlaw.com)
  • Specifically defined" means those amino acids other than "Xaa" and those nucleotide bases other than "n" defined in accordance with the World Intellectual Property Organization (WIPO) Handbook on Industrial Property Information and Documentation, Standard ST.25: Standard for the Presentation of Nucleotide and Amino Acid Sequence Listings in Patent Applications (1998), including Tables 1 through 6 in Appendix 2, herein incorporated by reference. (uspto.gov)
  • Modifications, e.g. , methylated bases, may be described as set forth in WIPO Standard ST.25 (1998), Appendix 2, Table 2, but shall not be shown explicitly in the nucleotide sequence. (uspto.gov)
  • three bases provide the code for each amino acid. (thefreedictionary.com)
  • Using the single-letter code, plug in the amino acids you wish to locate and Sequencher will find and highlight the bases within the sequence that code for those amino acids. (genecodes.com)
  • Sequencher can easily go to the next set of bases that comply with the search by selecting the Find Next button in the Find Amino Acids window. (genecodes.com)
  • In aqueous solutions they are dipolar ions (zwitterions, or hybrid ions) that react with strong acids or bases in a way that leads to the neutralization of the negatively or positively charged ends, respectively. (britannica.com)
  • Because of their reactions with strong acids and strong bases, the amino acids act as buffers-stabilizers of hydrogen ion (H + ) or hydroxide ion (OH − ) concentrations. (britannica.com)
  • Amino acids with side chains that are neither aromatic not aliphatic control the piano and organ: the nine non-hydrophobics the piano, and the four hydrophobics the organ. (stephan-zielinski.com)
  • The three amino acids with aliphatic side chains control the low synthesizer, while the four with aromatics control the percussion. (stephan-zielinski.com)
  • One of the most difficult steps in protein crystallography is model-building, which consists of constructing a backbone and then amino acid side chains into an electron density map. (ebscohost.com)
  • Comparison of the two sequences shows that the heavy-chain variable regions are similar in length to those of the light chains. (biochemj.org)
  • The existence of heavy chain variable region subgroups is also deduced, from a comparison of these two sequences with those of another γ 1 chain, Eu, a μ chain, Ou, and the partial sequence of a fourth γ 1 chain, Ste. Carbohydrate has been found to be linked to an aspartic acid residue in the variable region of one of the γ 1 chains, Cor. (biochemj.org)
  • It consists of 51 amino acids arranged in two chains (A and B chains). (frontiersin.org)
  • Using the information from these parasite populations, structural analysis reveals that polymorphic amino acids within TH2 and TH3 colocalize to one side of the protein, surround, but do not involve, the hydrophobic pocket in CS, and predominately involve charge switches. (nih.gov)
  • The successful application of support vector regression to the prediction of protein contact number reported here, together with previous applications of this approach to the prediction of protein accessible surface area and B-factor profile, suggests that a support vector regression approach may be very useful for determining the structure-function relation between primary protein sequence and higher order consecutive protein structural and functional properties. (biomedcentral.com)
  • Prediction of protein three-dimensional structure from primary sequence is the central problem in structural bioinformatics. (biomedcentral.com)
  • Taken together, this study represents the first application of selective unlabeling for sequence specific resonance assignments and opens up new avenues to using this methodology in protein structural studies. (iisc.ernet.in)
  • Based on sequence homology, the CipA CBD structure appears to be very similar to that of the previously elucidated family 3a CBD ( 15 , 17 ), including a nine-stranded jellyroll topology which exhibits distinctive structural elements consistent with family 3 CBDs. (asm.org)
  • The algorithm I used is a bit complicated, but just in case you're curious: since the gene is expressed as a surface protein antibodies can sense, it's considered as a string of amino acids. (stephan-zielinski.com)
  • It has been shown previously that NA is not required for virus replication or budding except in the final step of releasing the virus particles from the cell surface sialic acid receptor as well as preventing aggregation among the progeny virus particles ( 29 ). (asm.org)
  • Herein, the authors focus on the formation of self-assembled particles using an amphiphilic amino acid (AA) sequence derived by solid-phase peptide synthesis (SPPS) and describe its purifn. (unibas.ch)
  • Finer M, Aho S, Gerstenfeld L, Boedtker H, Doty P. Unusual DNA sequences located within the promoter region and the first intron of the chicken pro-alpha 1(I) collagen gene. (labome.org)
  • The sequence similarity of phosphatidylserine decarboxylase to the product of the ORF next to the ASS gene, together with the genomic context conservation, suggests that the ORF encodes archaetidylserine decarboxylase, which may transform archaetidylserine to archaetidylethanolamine. (hindawi.com)
  • So, the order of a sequence, if known, can be used to decipher the corresponding DNA code segment or gene that created it, through reverse engineering. (biologywise.com)
  • The sequence of amino acid arrangement in a protein, is itself created according to the translation of the genetic code of a gene. (biologywise.com)
  • The sequences of betasatellites have three major features-a single conserved (in both position and sequence) gene (referred to as βC1), a region of sequence rich in adenine (A-rich) and a sequence conserved among all betasatellites (known as the satellite conserved region (SCR) [ 12 , 14 ]. (mdpi.com)
  • The full-length palI gene sequence of Klebsiella oxytoca was cloned in E. coli DH5α. (osti.gov)
  • DNA sequencing result shows that palI gene of Klebsiella oxytoca was inserted in-frame in pET-32b. (osti.gov)
  • These enzymes were used as BLAST queries to search for similar sequences in a database containing 10 complete metazoan genomes. (mdpi.com)
  • This letter system was defined by the IUPAC and IUBMB in 1983, and is as follows: K-Lysine D-Aspartic acid E-Glutamic acid L-Leucine Therefore, the sequence in three letter code is: Lys-Asp-Glu-Leu. (wikipedia.org)
  • The amino acid sequence of the predicted active form of the enzyme is closely related to the partial sequences obtained for other kallikrein-like serine proteases including rat submaxillary gland kallikrein, pig pancreatic and submaxillary gland kallikreins, the gamma subunit of mouse nerve growth factor, and rat tonin. (docphin.com)