Amino acid residues protein clone cells. Medical search. Frequent questions

The isolation, characterization, and expression of a full-length cDNA encoding the human T cell glycoprotein CD6 is described. (embl.de)
A 1034-bp cDNA encoding duck IL-17 (duIL-17) was cloned from ConA-activated splenic lymphocytes of ducks. (usda.gov)
5ʹ-Rapid amplification of cDNA ends, done on poly(A)+ RNA from human U87 cells, was used to identify 420 bp of novel 5ʹ sequence of a PDE4B cAMP-specific phosphodiesterase (PDE). (ox.ac.uk)
A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. (rupress.org)
Analysis of the cDNA and deduced amino acid sequences revealed a high degree of identity with the transmembrane and cytoplasmic domains of previously described proteoglycans but a unique extracellular domain sequence. (rupress.org)
On Northern blots the cDNA hybridized to a single 5.6-kb mRNA that was present in Schwann cells, neonatal rat brain, rat heart, and rat smooth muscle cells. (rupress.org)
A 16-kD protein fragment encoded by the cDNA was expressed in bacteria and used to immunize rabbits. (rupress.org)
Scholars@Duke publication: Molecular cloning of a cDNA of a camptothecin-resistant human DNA topoisomerase I and identification of mutation sites. (duke.edu)
cDNA clones of topoisomerase I were isolated from the CPT-resistant and the parental CPT-sensitive cell lines, respectively. (duke.edu)
In this article, we report cloning of the rat apelin receptor cDNA. (nih.gov)
Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
Its cDNA sequence contained an open reading frame encoding a 431-amino acid peptide that showed a significant homology with members of the P2X family. (aacrjournals.org)
In this investigation, we cloned a novel cDNA for mouse brain sialidase and expressed the cDNA in COS-7 cells. (elsevierpure.com)
This 1699 bp cDNA codes for a 41.6 kDa protein consisting of 372 deduced amino acid residues. (elsevierpure.com)
In COS-7 cells transiently transfected with the cDNA, a 250-fold increase was observed in specific activity toward 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid. (elsevierpure.com)
Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. (rndsystems.com)

Also known as overlapping peptide scan or pepscan analysis, this technique uses a library of oligo-peptide sequences from overlapping and non-overlapping segments of a target protein, and tests for their ability to bind the antibody of interest. (wikipedia.org)

The lymphocyte glycoprotein CD6 contains a repeated domain structure characteristic of a new family of cell surface and secreted proteins. (embl.de)
NovoSeven is a vitamin K-dependent glycoprotein consisting of 406 amino acid residues. (drugbank.com)
PeproTech's Recombinant Human ROR1 is a glycoprotein containing 377 amino acid residues, and has a calculated molecular weight of approximately 42.4 kDa. (peprotech.com)

Molecular cloning, characterization and three-dimensional structure prediction of Lipoxygenase from Finger millet [Eleusine coracana (L.) Gaertn. (molquest.com)

The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) has recently emerged as a leading candidate antigen against the blood-stage human malaria parasite. (nature.com)
In immunology, epitope mapping is the process of experimentally identifying the binding site, or epitope, of an antibody on its target antigen (usually, on a protein). (wikipedia.org)
The extracellular domain of CD6 is significantly related to the extracellular domain of the human and mouse T cell antigen CD5, the cysteine-rich domain of the bovine and mouse type I macrophage scavenger receptor, the extracellular domain of the sea urchin spermatozoa protein that crosslinks the egg peptide speract, the mammalian complement factor 1, and the human lung tumor antigen L3. (embl.de)
It promotes the interaction of B-cell maturation antigen (BCMA) on multiple myeloma cells with CD3-expressing T cells. (drugbank.com)
Antigen presenting cells cultured with piperacillin for 24h also activated the clones with 2.8% Lys541 modification at this time-point. (liverpool.ac.uk)
Piperacillin-albumin conjugates that had levels and epitopes identical to those detected in patients were synthesized and purified, and were shown to stimulate T cells in an antigen processing dependent manner. (liverpool.ac.uk)
These clones were found to be activated via the direct binding of the drug (metabolite) to MHC molecules on antigen presenting cells. (liverpool.ac.uk)
Functional recombinant FRβ protein was produced in insect cells and used as antigen to isolate a mAb, m909, from a human naïve Fab phage display library. (biomedcentral.com)
CD20 is a B-cell-specific antigen expressed on malignant FL cells. (spandidos-publications.com)

The encoded protein, predicted to consisted of 169 amino acids, displayed a molecular weight of 18.8 kDa, a 29 residue NH2-terminal signal peptide, a single potential N-linked glycosylation site, and 6 cysteine residues conserved with mammalian IL-17s. (usda.gov)

This protease activity has recently been ascribed to serine metalloprotease(s), including pregnancy-associated plasma protein-A (PAPP-A), which was first detected in human follicular fluid nearly 20 yr ago. (bioone.org)
The cytoplasmic domain of CD6 contains two serine residues, one or both of which are substrates for phosphorylation during T cell activation. (embl.de)
In agreement with published findings regarding different HEA and LEA B. napus cultivars, comparison of FAE1 protein sequences from HEA and LEA Brassicaceae revealed one crucial amino acid difference: the serine residue at position 282 of the HEA FAE1 sequences is substituted by phenylalanine in LEA B. napus cv. (unboundmedicine.com)
Using site directed mutagenesis, the phenylalanine 282 residue was substituted with a serine residue in the FAE1 polypeptide from B. napus cv. (unboundmedicine.com)

Primer extension and polymerase chain reaction amplification were used to obtain additional 5' protein coding sequences. (rupress.org)
Although we detected no genetic alteration in the coding sequences, one of four rhabdomyosarcoma cell lines examined had completely lost expression of this gene. (aacrjournals.org)
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
The betA gene sequence reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)
Moreover, the sequence analysis of choline dehydrogenase from our isolate shows several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)

Within the V region families, certain amino acid residues occurring at particular positions in the L-chain sequence render those chains more amyloidogenic, with a combination of such residues increasing the chances of a particular L-chain protein being associated with tissue amyloid deposition. (medscape.com)
Why certain amino acid and glycosylation characteristics in L chains predispose to amyloid formation remains unknown. (medscape.com)

the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)

abstract = "Sialidase (EC 3.2.1.18) catalyzes the release of sialic acid from sialo-oligosaccharides, gangliosides, or sialo-glycoproteins. (elsevierpure.com)
volume = {285}, number = {32}, pages = {24307--24312}, abstract = {Inhibitory serpins are metastable proteins that undergo a substantial conformational rearrangement to covalently trap target peptidases. (cnrs.fr)

The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr-576 and Tyr577) in the kinase domain activation loop. (fishersci.com)
In addition, phosphorylation of other Tyr residues (Tyr-925, and Tyr-861) creates binding sites for SH2 domains of intracellular signaling molecules such as Src, PI3 kinase, and Grb2. (fishersci.com)
cAMP-dependent protein kinase (PKA) mediates intracellular signaling in response to neurotransmitters such as dopamine and norepinephrine. (yu.edu)
A novel brain-enriched RII{dollar}\beta{dollar}-binding protein, AKAP (A Kinase Anchor Protein), has been identified and characterized in two species in the Rubin laboratory (Bregman et al. (yu.edu)
Recent reports demonstrate that the expression of protein kinase C alpha (PKCα) in triple-negative breast cancer (TNBC) correlates with decreased survival outcomes. (oncotarget.com)
Receptor-tyrosine-kinase-like orphan receptor 1 (ROR1) is a tumor-associated, surface protein predominantly expressed during embryogenesis, where it is involved in organ morphogenesis, nervous system development, and neural progenitor cell maintenance and survival. (peprotech.com)
First identified during PCR-based cloning of a human neuroblastoma cell line in search of tyrosine kinases similar to tropomyosin-receptor-kinase (Trk) neurotropic receptors, ROR1, along with the related receptor tyrosine kinase (RTK) ROR2, was catalogued as an "orphan" receptor due to the fact its related ligand remained elusive. (peprotech.com)
Proteins kinase CK2 (previously called casein kinase 2) is a pivotal and ubiquitously expressed member of the protein CX-6258 manufacture kinase CMGC subfamily which includes cyclin-dependent kinases (CDKs) mitogen-activated protein kinases (MAPKs) glycogen synthase kinases (GSKs) and CDK-like kinases (CLKs). (immune-source.com)
is a constitutively active protein kinase and is further fully activated by association with CK2? (immune-source.com)

G protein-coupled receptors [GPCRs]) and multi-subunit proteins (e.g., ion channels) are key targets of drug discovery. (wikipedia.org)
Cloning of a novel bacteria-binding receptor structurally related to scavenger receptors and expressed in a subset of macrophages. (embl.de)
Macrophage scavenger receptors are integral membrane proteins whose ability to bind and degrade modified LDL has implicated them in the process of atherosclerotic foam cell formation. (embl.de)
On these cells, it binds to receptors that recognize MCP-1, MCP-3, and RANTES. (rupress.org)
Clones expressed chemokine receptors consistent with a mixed Th1/Th2 response. (liverpool.ac.uk)
In addition to RFC and PCFT, a limited number of cells express folate receptors (FRs) that can mediate unidirectional transportation of folates into cells. (biomedcentral.com)

The gene for bovine AKAP 75 was cloned and expressed in mammalian cells (Hirsch et al. (yu.edu)
We report on the cloning and sequencing of two Tranosema rostrale ichnovirus (TrIV) genes, and assess their relatedness to TrV1, the gene encoding the most abundant TrIV transcript in last-instar Choristoneura fumiferana larvae parasitized by T. rostrale . (gc.ca)
In 1992, Douglas Prasher was successful in cloning the wild-type GFP gene, and in 1994, Martin Chalfie expressed the coding sequence in E. coli and C. elegans cells. (ibs.fr)
Through cloning of functional p53-binding sites (p53-tagged sites) from the human genome, we isolated a novel gene inducible by wild-type p53. (aacrjournals.org)
This protein also revealed a similarity to RP-2 , a gene activated in thymocytes undergoing programmed cell death. (aacrjournals.org)
synthesized very long chain monounsaturated fatty acids that are not normally found in yeast, while fatty acid profiles of yeast cells expressing the FAE1 gene from LEA B. napus were identical to control yeast samples. (unboundmedicine.com)
Westar, the mutated gene was expressed in yeast and GC analysis revealed the presence of very long chain monounsaturated fatty acids (VLCMFAs), indicating that the elongase activity was restored in the LEA FAE1 enzyme by the single amino acid substitution. (unboundmedicine.com)
The gene bgl, encoding β-glucosidase, was cloned and sequenced. (hud.ac.uk)
Expression of pET-bgl together with a chaperone gene cloned in vector pGro7 in Escherichia coli dramatically enhanced the crude enzyme activity to a specific activity of 256.3 U/mg wet cells, which resulted in a 9.2-fold increase of that obtained from the expression without any chaperones. (hud.ac.uk)
CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. (rndsystems.com)
Clonal plasma cell proliferative diseases in which the V l 6 gene is expressed are always associated with amyloid deposition. (medscape.com)

The most common light chain-type amyloidosis precursor proteins are L chains of the lambda (l) class. (medscape.com)

Duck IL-17 shared 84% amino acid sequence identity to the previously described chicken IL-17 (chIL-17) and 36-47% to mammalian homologues and the open reading frame 13 of Herpesvirus saimiri (HVS 13). (usda.gov)

This identified an open reading frame encoding a putative 721-residue 'long-form' PDE4B splice variant, which we term HSPDE4B3. (ox.ac.uk)
The putative extracellular domain contains three potential glycosaminoglycan attachment sites, as well as a domain rich in Thr and Pro residues. (rupress.org)
The peptide apelin, recently isolated from bovine stomach tissue extracts, has been identified as an endogenous ligand of the human putative receptor protein related to the angiotensin receptor AT(1) (APJ). (nih.gov)

This work identifies Drosophila S2 cells as a clinically-relevant platform suited for the production of 'difficult-to-make' proteins from Plasmodium parasites, and identifies a PfRH5 sequence variant that can be used for clinical production of a non-glycosylated, soluble full-length protein vaccine immunogen. (nature.com)
Immunogen: Recombinant fragment (87 amino acid residues around 1-150) of human ARG1 protein. (scytek.com)

Complex target antigens, such as membrane proteins (e.g. (wikipedia.org)
Membrane proteins frequently have short antigenic regions (epitopes) that fold correctly only when in the context of a lipid bilayer. (wikipedia.org)
As a result, mAb epitopes on these membrane proteins are often conformational and, therefore, are more difficult to map. (wikipedia.org)
Tandem repeats of SRCR domains are common in the membrane bound proteins. (embl.de)
A novel murine plasma membrane protein has been identified in subpopulations of macrophages. (embl.de)
Transfected COS cells synthesized a native trimeric plasma membrane protein that bound labeled bacteria and acetylated LDL, but not yeast or Ficoll. (embl.de)
The results suggest that the novel protein is a macrophage-specific membrane receptor with a role in host defense, as it shows postnatal expression in macrophages, which are considered responsible for the binding of bacterial antigens and phagocytosis. (embl.de)
The predicted CD6 sequence is 468 amino acids long, with the typical features of a type I integral membrane protein. (embl.de)
The deduced amino acid sequence predicted a 353 amino acid polypeptide with a single membrane spanning segment and a 34 amino acid hydrophilic COOH-terminal cytoplasmic domain. (rupress.org)
Therefore, how a complex LHCGR protein, embedded within the membrane lipid bilayer, could be secreted as soluble receptor remained unexplained. (biomedcentral.com)
In normal cells, folates are taken in through the reduced folate carrier (RFC) or proton-coupled folate transporter (PCFT), which are membrane-spanning proteins that facilitate bidirectional transportation of reduced folate across the plasma membrane and endosome membranes [ 1 ]. (biomedcentral.com)
Numerous in vitro experiments have indicated that betaine acts as an osmoprotectant by stabilizing both the quaternary structure of proteins and cellular membrane against the adverse effects of high salinity and extreme temperatures ( Gorham, 1995 ). (scialert.net)

Specificity: This antibody recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). (scytek.com)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
Manufactured using AbAb's Recombinant Platform with variable regions (i.e. specificity) from the chicken B-cell clone 4S1. (absoluteantibody.com)

Camptothecin (CPT), a plant alkaloid with antitumor activity, is a specific inhibitor of eukaryotic DNA topoisomerase I. We have previously isolated and characterized a CPT-resistant topoisomerase I isolated from a CPT-resistant human leukemia cell line, CPT-K5. (duke.edu)
Members of the discoidin (DS) domain family, which includes the C1 and C2 repeats of blood coagulation factors V and VIII, occur in a great variety of eukaryotic proteins, most of which have been implicated in cell-adhesion or developmental processes. (embl-heidelberg.de)
and it has been within many diseases especially cancer rendering it an interesting focus on inside CX-6258 manufacture the druggable category of eukaryotic proteins kinases (Pagano et al. (immune-source.com)

The entire thyroglobulin molecule contains about 5000 amino acids, and it provides the conformation required for tyrosyl coupling and iodide organification necessary for the formation of diamino acid thyroid hormones. (medscape.com)

96% amino acid identity with HSPDE4B3 and is proposed to reflect the rat homologue of this human enzyme and is thus called RNPDE4B3. (ox.ac.uk)
The resistance index (greater than 125) of the CPT-K5 fusion topoisomerase I is similar to that of the native CPT-K5 topoisomerase I. These results indicate that either or both of the two amino acid changes identified in the mutant enzyme is responsible for the resistance to CPT. (duke.edu)
Amino-acid sequence and structure comparison suggested that the enzyme belongs to a group of enzymes classified as archaeal Holliday junction-resolving enzymes, which are typically divalent metal ion-binding dimers that are able to cleave X-shaped dsDNA-Holliday junctions (Hjs). (iucr.org)
Author Manuscript and RNA repair protein AlkB-derived enzyme mixture was first used to remove methylations at the Watson-Crick face. (cdc.gov)

A dominant antigenic epitope on SARS-CoV spike protein identified by an avian single-chain variable fragment (scFv)-expressing phage. (absoluteantibody.com)
Before the discovery that the major fibril component in these patients was an immunoglobulin fragment, patients with light chain-type amyloidosis were described as having primary amyloidosis (primary in the sense of idiopathic) or, when the burden of monoclonal plasma cells was large, myeloma-associated amyloidosis. (medscape.com)

Furthermore, a minor splice variant lacking a part of exon 1 that would encode residues corresponding to transmembrane domain M1 was relatively more abundant in two of seven sarcoma cell lines, one of which was derived from a rhabdomyosarcoma, and the other was derived from an osteosarcoma. (aacrjournals.org)

This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the "Focal Adhesion Targeting" sequence (FAT). (fishersci.com)
This suggests that the 75-residue form with the characteristic NH2 terminus of an MCP is the biologically relevant species. (rupress.org)
In contrast, deletion of the first seven amino acids of K17F or substitution of phenylalanine by an alanine residue at the C-terminus completely abolished the activity of the peptide. (nih.gov)
2 and methods 2.1 Construction of the expression plasmid The coding region corresponding to amino-acid residues Met1-Gln334 of human CK2?2 was amplified by the polymerase chain reaction (PCR) and cloned into the vector pGEX-6P-1 (GE Healthcare) at the restriction sites BamHI-EcoRI providing the construct in a GST-fused form at the N-terminus. (immune-source.com)
The fragments always include the amino terminus of the chain and range in mass from 5000 to 16,000 d. (medscape.com)

J Cell Biol (1992) 117 (1): 191-201. (rupress.org)

2.2 Protein expression and purification Escherichia coli strain HMS174 (DE3) cells (Novagen) were transformed with pGEX-hCK2?2. (immune-source.com)
Escherichia coli JM109 and plasmid pTZ57R/T (MBI Fermentas, Hanover, Maryland, USA) were used as transformation host and cloning vector, respectively. (scialert.net)

After maturation, these three amino-acids form a para-HydroxyBenzylideneDimethylImidazolinone (p-HBDI) structure, responsible for the light absorption and emission properties of the protein. (ibs.fr)
One of the most interesting features of the GFP chromophore is that it does not require a catalytic system to be formed, but is autocatalytically assembled after protein production, requiring only molecular oxygen as a cofactor for its full maturation. (ibs.fr)

Anti-merozoite vaccine studies have long relied on the standardized in vitro assay of growth inhibition activity (GIA) 12 , whereby purified IgG antibodies are tested against parasites cultured in human red blood cells (RBC) in the absence of other cell types. (nature.com)
Human alveolar adenocarcinoma epithelial A549 cells were maintained in Ham's F12-K containing 10% FCS. (cdc.gov)
As shown for the human APJ receptor, the rat apelin receptor expressed in the cell line was negatively coupled to adenylate cyclase. (nih.gov)
By challenging HCT116 human cells with either synthetic dsRNA or Sindbis virus (SINV), we identified the heparan sulfate (HS) pathway as a crucial factor for dsRNA entry, and we validated SINV dependency on HS. (cnrs.fr)
Using a genome-wide screening approach, we set to identify genes involved in dsRNA entry, sensing, and apoptosis induction in human cells. (cnrs.fr)
We examined the possibility that the C2 domain, amino acid residues 2173-2332, of factor VIII (fVIII) contains a binding site for von Willebrand factor (vWf) to clarify previous data showing that some monoclonal and human inhibitor antibodies with epitopes in C2 prevent fVIII-vWf binding. (embl-heidelberg.de)
The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. (rndsystems.com)
Proteins containing CCCH Znf domains include Znf proteins from eukaryotes involved in cell cycle or growth phase-related regulation, e.g. human TIS11B (butyrate response factor 1), a probable regulatory protein involved in regulating the response to growth factors, and the mouse TTP growth factor-inducible nuclear protein, which has the same function. (embl.de)
Another protein containing this domain is the human splicing factor U2AF 35kDa subunit, which plays a critical role in both constitutive and enhancer-dependent splicing by mediating essential protein-protein interactions and protein-RNA interactions required for 3' splice site selection. (embl.de)
Immunoglobulin L and H chains are two of 20 different fibril proteins that have been described in human amyloidosis. (medscape.com)
Although 15% of human L chains bear sugar residues, almost one third of amyloidogenic L chains are glycosylated. (medscape.com)
We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. (biomedcentral.com)
Piperacillin is β-lactam antibiotic that is known to covalently modify lysine residues on proteins such as human serum albumin. (liverpool.ac.uk)
aegypti ingest blood from a viremic human and the virus infects midgut epithelial cells. (cdc.gov)

With the aid of mass spectrometry, we identified the main lysine residues that piperacillin modifies and synthesized a piperacillin modified peptide incorporating amino acid residues of albumin to establish a standard curve for quantification of binding. (liverpool.ac.uk)

MCP-4 shares the pyroglutamic acidproline NH2-terminal motif and 56-61% sequence identity with the three known monocyte chemotactic proteins and is 60% identical to eotaxin. (rupress.org)

Experimental epitope mapping data can be incorporated into robust algorithms to facilitate in silico prediction of B-cell epitopes based on sequence and/or structural data. (wikipedia.org)
B-cell epitope mapping studies suggest that most interactions between antigens and antibodies, particularly autoantibodies and protective antibodies (e.g., in vaccines), rely on binding to discontinuous epitopes. (wikipedia.org)
Epitope mapping can reveal how a mAb exerts its functional effects - for instance, by blocking the binding of a ligand or by trapping a protein in a non-functional state. (wikipedia.org)
Many therapeutic mAbs target conformational epitopes that are only present when the protein is in its native (properly folded) state, which can make epitope mapping challenging. (wikipedia.org)
Epitope data have been central to several high-profile legal cases involving disputes over the specific protein regions targeted by therapeutic antibodies. (wikipedia.org)
Finally, neither technique can distinguish key epitope residues (energetic "hot spots") for mAbs that bind to the same group of amino acids. (wikipedia.org)
This clone recognizes and binds an epitope present between amino acid residues 456-650 of the spike protein (Lee et al. (absoluteantibody.com)

The secreted proteins were folded and sensitive to glycosidases suggesting N-linked glycosylation. (biomedcentral.com)
Among the four isoforms of FRs identified (α, β, γ, and δ), α and β isoforms of FR are glycosylphosphatidylinositol (GPI)-anchored proteins with two N-glycosylation sites, and both have high affinity (K D of approximately 1 nM) for folate [ 2 ]. (biomedcentral.com)

N-terminal elongation of K17F with a tyrosine or the N-terminal deletion of the first four amino acids did not modify the inhibitory action of K17F on cAMP production. (nih.gov)
Thyroglobulin contains 2 subunits, which together contain 115 tyrosine residues, each of which is a potential site for iodination. (medscape.com)

In RT4-E5 cells, glucocorticoids elicited an increase in the abundance of AKAP 150 polypeptide, whereas RII{dollar}\beta{dollar} accumulated following forskolin administration. (yu.edu)

May bind bacterial antigens in the protein MARCO. (embl.de)
FAK's ability to bind numerous structural and signaling proteins via a variety of interactions is important for FAK activation level, and for FAK interaction with a variety of substrates localized to sites of cell adhesion. (fishersci.com)
They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
The present study demonstrated that CA125 could bind to RTX and reduce its tumor cell killing activity. (spandidos-publications.com)
To cause sensitization, a chemical must bind to macromolecules (proteins) in the skin. (cdc.gov)

293T cells and chicken fibroblast DF-1 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS). (cdc.gov)

LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. (biomedcentral.com)

The antibodies induced by one protein variant were shown to be qualitatively similar to responses induced by other vaccine platforms. (nature.com)
In this regard, the Amgen v. Sanofi/Regeneron Pharmaceuticals PCSK9 inhibitor case hinged on the ability to show that both the Amgen and Sanofi/Regeneron therapeutic antibodies bound to overlapping amino acids on the surface of PCSK9. (wikipedia.org)
COS cells transfected with the CD6 clone express a 90-kD protein that reacts with all available anti-CD6 monoclonal antibodies. (embl.de)
The resulting antibodies reacted on immunoblots with the core protein of a detergent extracted heparan sulfate proteoglycan. (rupress.org)
When the anti-core protein antibodies were used to stain tissue sections immunoreactivity was present in peripheral nerve, newborn rat brain, heart, aorta, and other neonatal tissues. (rupress.org)

As shown on the figure (so called Jablonski diagram), when a molecule (e.g. a fluorescent protein) in the electronic ground state absorbs a photon of suitable wavelength, it accesses the first electronically excited state. (ibs.fr)
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)

These changes are thought to lead to tumor-microenvironment interactions via factors secreted by stromal or epithelial cells in response to lymphoma cell-produced cytokines ( 7 ). (spandidos-publications.com)

The particulate nature of PKA-II{dollar}\beta{dollar} is due to interactions between the regulatory subunit (RII{dollar}\beta{dollar}) and anchor proteins in intracellular structures. (yu.edu)
While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)
co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)
The hormonal activation occurs through specific ligand-receptor interactions on the surface of the target cells. (biomedcentral.com)
Together, our results define in immunological, chemical and quantitative terms the drug immune receptor interactions that can drive a T-cell response. (liverpool.ac.uk)

Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. (rndsystems.com)
A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. (rndsystems.com)

it encodes a 102 amino acid protein showing 79% similarity to TrV1. (gc.ca)

The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. (rndsystems.com)
Regarding piperacillin, we characterized the piperacillin reactive T-cells response of piperacillin hypersensitive patients with cystic fibrosis in terms of proliferation, cytokine secretion in addition to TCR-Vβ and chemokine receptor expression. (liverpool.ac.uk)

Wnt-5a has since been suggested as a candidate ligand for ROR1, and ROR1 has been implicated to function as a pseudokinase, promoting proliferation and resistance to apoptosis in cancer cells through interaction with Wnt-5a, and TCL1-co-activation of AKT. (peprotech.com)
A different experimental approach by West and Cooke [ 10 ] demonstrated the secretion of M r 80-90K hCG-LH receptor complex into the culture media from ligand-induced rat and mouse leydig cells. (biomedcentral.com)
instead, a specific intra-cellular role of truncated receptor in regulating the cell surface expression of a full-length ligand-binding Lhcgr has been proposed [ 15 ]. (biomedcentral.com)

This allowed us to determine that the heparan sulfate pathway and the conserved oligomeric Golgi complex are key determinants allowing entry of both dsRNA and viral nucleic acid leading to cell death. (cnrs.fr)

An antibody is used to precipitate a protein along with bound proteins. (genomicglossaries.com)
The IgG1 m909 showed much higher (femtomolar) avidity as measured by ELISA, and it bound to FRβ positive cells in a dose-dependent manner, but not to parental FRβ negative cells. (biomedcentral.com)

Tristetraprolin (TTP), the prototype of a class of CCCH zinc finger proteins, is a phosphoprotein that is rapidly and transiently induced by growth factors and serum in fibroblasts. (embl.de)

However it has proved challenging to identify a heterologous expression platform that can produce a soluble protein-based vaccine in a manner compliant with current Good Manufacturing Practice (cGMP). (nature.com)
The neuron-like cell line RT4-E5 and the glial-like cell line RT4-D6 were used to study the hormonal regulation of AKAP 150 expression. (yu.edu)
Briefly, eight RNA polymerase I plasmids (for the synthesis of the eight influenza A viral RNAs) together with plasmids for the expression of the PB2, PB1, PA, and NP proteins derived from the influenza A virus strain A/WSN/33 (H1N1) were transfected into 293T cells. (cdc.gov)
green fluorescent protein (GFP) fusions revealed egl-2 expression in a subset of neurons and muscles that could explain the mutant behavioral defects. (jneurosci.org)
This study shows that the expression of both myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) correlates with PKCα expression in TNBC. (oncotarget.com)
The results suggest that P2XM may play a significant role in the proliferation and/or differentiation of skeletal muscle cells and that its altered expression may be involved in the development of some sarcomas. (aacrjournals.org)
Virtually absent from normal pediatric and adult tissues, with the exception of low-level expression in a subset of immature b cell precursors known as hematogones and adipocytes, ROR1 is notably overexpressed, and considered a survival factor, in a number of B lymphoid and epithelial malignancies: including chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), marginal zone lymphoma, lung adenocarcinoma. (peprotech.com)
Additionally, the truncated Lhcgr, misrouted the full-length Lhcgr in ER, thereby decreasing the cell surface expression of the mature functional receptor. (biomedcentral.com)
The detection of GPR15 in the colon suggests it may be important for transmission of HIV and SIV, and the authors speculate that the high expression in the colon suggests it may be present in non-lymphoid cells [ 11 ]. (guidetopharmacology.org)
Anatomical profiling of G protein-coupled receptor expression. (guidetopharmacology.org)
Piperacillin-albumin conjugate clones expressed a restricted pattern of TCR-Vβ with high expression of TCR-Vβ 9 in many clones. (liverpool.ac.uk)
Most clones were CD4 T-cells and they displayed a restricted pattern of TCR-Vβ expression. (liverpool.ac.uk)

Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
A ribonuclease protection assay was used to quantitate levels of the core protein mRNA. (rupress.org)
Southern and Northern analyses, using DNA and RNA extracted from infected Cf-124T cells, revealed the presence of both TrV1- and TrV4-carrying genome segments and transcripts, but neither DNA, at least in episomal form, nor mRNA persisted for more than a few days p.i. (gc.ca)

1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

Conformational epitopes epitopes are formed by amino acids that are nearby in the folded 3D structure but distant in the protein sequence. (wikipedia.org)

The RII-binding protein is a neuronal dendritic protein that is highly expressed in forebrain regions such as basal ganglia and cerebral cortex. (yu.edu)
The results suggest that the bifunctional AKAP 150 protein tethers PKA-II{dollar}\beta{dollar} to the dendritic cytoskeleton, thereby creating a discrete target site for the reception and propagation of signals carried by cAMP. (yu.edu)
Piperacillin responsive T-cells displaying a similar phenotype were also generated from naïve healthy volunteers using a dendritic cell-T-cell priming technique. (liverpool.ac.uk)

Genomic fatty acid elongation 1 (FAE1) clones from high erucic acid (HEA) Brassica napus, Brassica rapa and Brassica oleracea, and low erucic acid (LEA) B. napus cv. (unboundmedicine.com)

Thus, for the first time, the low erucic acid trait in canola B. napus can be attributed to a single amino acid substitution which prevents the biosynthesis of the eicosenoic and erucic acids. (unboundmedicine.com)

Cloned and expressed in hamster kidney cells, the protein is catalytically active in a two-chain form. (drugbank.com)
Amino acid sequence identities to rat and hamster sialidases (84% and 77%, respectively) suggest that this form of sialidase is conserved in rodents. (elsevierpure.com)

In situ hybridization and immunohistochemical staining have localized the protein to a subset of macrophages in the marginal zone of the spleen and the medullary cord of lymph nodes. (embl.de)

Studies utilizing cloned native and mutant forms of the scavenger receptor have provided insights into the structural basis for their function. (embl.de)
Subsequently a stable CHO cell line expressing the receptor fused at its C-terminal part with the enhanced green fluorescent protein (EGFP) was established, allowing to verify its cell surface distribution and to determine the affinity of various apelin and angiotensin fragments on the cloned receptor. (nih.gov)
The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)
Folate receptor beta (FRβ) is only detectable in placenta and limited to some hematopoietic cells of myeloid lineage in healthy people. (biomedcentral.com)

The original antibody was generated by immunizing chicken with recombinant spike proteins. (absoluteantibody.com)
This antibody binds the amino acid residues 456-650 of the spike protein. (absoluteantibody.com)
The binding of this antibody to SARS-CoV infected Vero E6 cells was confirmed using ELISA and immunocytochemical staining. (absoluteantibody.com)
Antibody-dependent cell-mediated cytotoxicity (ADCC) was evaluated with FRβ positive CHO cells as target cells and isolated peripheral blood monocytes as effector cells in an in vitro assay. (biomedcentral.com)
The mechanisms of action by which RTX kills FL cells include complement-dependent cytotoxicity (CDC) ( 3 ), antibody-dependent cellular cytotoxicity (ADCC) ( 4 , 5 )-herein referred to as 'effector function' mechanisms-as well as CD20 signaling effects ( 6 ). (spandidos-publications.com)

However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)

Nucleic Acids Res 19, no. 1 (January 11, 1991): 69-75. (duke.edu)

Subcellular localization prediction of predicted BoGST proteins was performed using Protcomp 9.0 from Softberry [96]. (molquest.com)

Cell surface-attached carbohydrate-binding domain, present in eukaryotes and assumed to have horizontally transferred to eubacterial genomes. (embl-heidelberg.de)

The binding site in Pra1 is in the C-terminal region of this 299 aa protein ( Łoboda and Rowińska-Żyrek 2017 ). (tcdb.org)

For FPs, one often compares the MB to that of enhanced Green Fluorescent Protein (EGFP), which is about 30000 M -1 .cm -1 . (ibs.fr)

Mouse brain sialidase has two amino acid sequence motifs common to bacterial sialidases: the 'F/YRIP' motif and the 'Asp-box' motif. (elsevierpure.com)

GSD type II, also known as alpha glucosidase deficiency (GAA, acid maltase deficiency) or Pompe disease, is a prototypic lysosomal disease. (medscape.com)

For example, cell adhesion to a fibronectin substratum involves concurrent activation of Src and phosphorylation of the FAK activation loop. (fishersci.com)
Thus, FAK activity is regulated by a complex set of phosphorylation sites, and thisphospho-regulation could be important for cell motility, cell growth, cytoskeletal organization, and adhesion-dependent cell survival. (fishersci.com)
In the Dictyostelium discoideum (Slime mold) cell adhesion protein discoidin, a related domain, named discoidin I-like domain, DLD, or DS, has been found which shares a common C-terminal region of about 110 amino acids with the FA58C domain, but whose N-terminal 40 amino acids are much less conserved. (embl-heidelberg.de)

Anatomy12

Organisms14

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes90

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science6

Health Care3

CDNA16

Overlapping peptide1

Glycoprotein3

Characterization1

Antigen9

Cysteine residues1

Serine4

Sequences5

Certain amino acid2

Peptides1

Abstract2

Kinase9

Receptors6

Gene11

Precursor1

Mammalian1

Putative3

Immunogen2

Membrane12

Specificity3

Eukaryotic3

Conformation1

Enzyme4

Fragment2

Transmembrane1

Terminus5

19921

Escherichia2

Maturation2

Human14

Lysine1

Sequence identity1

Epitope7

Glycosylation2

Tyrosine2

Polypeptide1

Bind5

Fetal1

Extracellular domain1

Antibodies5

Molecule2

Epithelial cells1

Interactions5

Lymphocytes2

Encodes1

Chemokine2

Ligand3

Nucleic acid1

Bound2

Transiently1

Expression12

MRNA4

Molecular weight1

Conformational1

Dendritic3

GENOMIC1

Substitution1

Hamster2

Situ hybridization1

Receptor5

Antibody5

Monocytes1

19911

Prediction1

Eukaryotes1

20171

Green fluores1

Motifs1

Lysosomal1

Adhesion3