• The green synthesized Ag NPs were characterized by diverse techniques such as powder X-ray diffraction (XRD), UV-visible, Fourier transform-infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM) coupled with X-ray energy dispersive spectroscopy (EDS) techniques . (omicsonline.org)
  • He has developed new experimental techniques involving 1 and 2 dimensional high-resolution and high-pressure nuclear magnetic resonance spectroscopy to study proteins and protein folding. (wikipedia.org)
  • Techniques include Raman and Rayleigh laser scattering methods for spectroscopy, Fourier transform infrared spectroscopy (FTIR) and photo-chemically induced dynamic nuclear polarization (Photo-CIDNP). (wikipedia.org)
  • The structural and conformational changes of proteins are of great concern and several spectroscopic methods e.g. circular dichroism, 74-76 Fourier-transformed infrared spectroscopy (FTIR), 77,78 NMR, 79 small-angle X-ray scattering (SAXS) 80 and electron paramagnetic resonance 81 have been used. (azpdf.net)
  • We provide thorough characterization of the effect of pH, effect of salts and protein conformation on self-assembled collagen samples via several complementary biophysical techniques, including circular dichroism (CD), Fourier Transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), atomic force microscopy (AFM), scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA) and thermogravimetric analysis (TGA). (biomedcentral.com)
  • The chemical nature of the non-tryptophan (non-Trp) fluorescence of porcine and human eye lens proteins was identified by Mass Spectrometry (MS) and Fluorescence Steady-State and Lifetime spectroscopy as post-translational modifications (PTM) of Trp and Arg amino acid residues. (edinst.com)
  • They were identified as silk based on structures by morphological observation and chemical components revealed by Fourier Transform Infrared spectroscopy (FTIR). (springeropen.com)
  • The article describes detailed procedure for the isolation (by Column Chromatography) and structural characterization (by FTIR, UV-Visible Spectroscopy and HPTLC) of β-sitosterol from Muntingia Calabura bark. (scientificarchives.com)
  • Dr. Wang's research focuses on the application of peptide synthesis and FTIR spectroscopy in the pathology study of Alzheimer's disease and Parkinson's disease. (mtsu.edu)
  • 82 The basic idea is that if the Trp is buried deep inside the hydrophobic protein moiety, it normally displays an emission maximum at 308 nm but whenever the protein becomes unfolded and the Trp residue is exposed to water and undergoes a red shifting to 355 nm. (azpdf.net)
  • Besides autodock studies, Schiffer-Edmundson helical wheel diagrams of LTB in α-helix domain suggested that LTB hydrophobic core is a highly effective region, which was able to form favorable non-polar interactions of the protein's binding surface (with amino acids residues such as Tyr, Leu, Ile) with EPS-LM. (hal.science)
  • Our results favor the "hydrophobic collapse" product of protein folding for PfP2. (dna-alkylating.com)
  • OPG presented spherical shape, smooth surface, smaller particle size and polydispersity index [51.0 (6.07) nm and 0.40 (0.05)], and better chemical interaction between the nonpolar amino acids and the hydrophobic oil chain. (biomedcentral.com)
  • Other polypeptides and proteins , such as amylin and the Alzheimer's beta protein do not have a simple consensus sequence and are thought to operate by hydrophobic association. (wikidoc.org)
  • Among the hydrophobic residues, aromatic amino acids are found to have the highest amyloidogenic propensity. (wikidoc.org)
  • The docking simulations study revealed that these synthesized compounds displayed hydrogen bonding, electrostatic and hydrophobic interactions with active site residues. (biomedcentral.com)
  • Insight into the Function of Active Site Residues in the Catalytic Mechanism of Human Ferrochelatase. (uga.edu)
  • Isotope-edited FTIR in H2O: Determination of the conformation of specific residues in. (mtsu.edu)
  • When peptides are in a beta-sheet conformation, particularly when the residues are parallel and in-register (causing alignment), glutamines can brace the structure by forming intrastrand hydrogen bonding between its amide carbonyls and nitrogens . (wikidoc.org)
  • These include electron paramagnetic resonance (EPR), resonance Raman, FTIR, and UV/visible/near-IR absorption and natural and magnetically induced circular dichroism (CD and MCD). (uga.edu)
  • The GFP chromophore is observed in the visible region at 400 nm and the absorption bands due to the aromatic amino acid residues are observed in the near-UV region between 250 and 300 nm. (jascoinc.com)
  • In a complementary technique, peptide mass fingerprinting involves digestion of the native and modified protein with a protease such as trypsin. (chksignal.com)
  • Each hormone peptide has its own class B type G-protein coupled receptor 19 , 21 functioning along identical receptor activating schemes (Fig. 1c ). (nature.com)
  • Notably, the lifetime constants of these residues in a model peptide were considerably greater than those of their "free" counterparts. (edinst.com)
  • The latter facilitates the simultaneous use of two orthogonal split inteins within the same peptide or protein, an approach termed tandem protein trans-splicing (tPTS). (biorxiv.org)
  • The described setup and experimental design enable real-time monitoring of substrate turnover in light-activated enzymes on a molecular scale, thus opening the pathway to a deeper understanding of enzyme activity and protein-protein interactions. (elifesciences.org)
  • FTIR experimental studies are supported by the results of the static density functional theory calculations and Car-Parrinello molecular dynamics simulations. (researchgate.net)
  • A molecular dynamic (MD) modeling approach was applied to evaluate the effect of external electric field on gliadin protein structure and surface properties. (mdpi.com)
  • This review aims to link recent molecular data, often translated into amino acid sequences and predicted three dimensional structural motifs, to known mechanical properties. (bioone.org)
  • They were composed of glucose and mannose and 10 different amino acids and with molecular weights of 18,000 and 8000 Da, respectively. (scirp.org)
  • P contained 66.1% carbohydrate, 35.3% protein and 2.1% uronic acid and ranged in molecular weight from 850 to 20,000 Da. (scirp.org)
  • Moreover, a molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in Fe-S cluster biogenesis proteins. (uga.edu)
  • Research on Fe-S cluster biosynthesis in the Johnson group currently involves investigating the molecular mechanisms of Fe-S cluster assembly on scaffold proteins and the subsequent intact cluster transfer to acceptor or carrier proteins. (uga.edu)
  • Transcriptomic analysis of the two recombinant strains in comparison with the control strain showed that the correct folding of proteins assisted by molecular chaperones played a key role in the improvement of FumDM expression and its enzyme activity. (inra.fr)
  • Alginate is an anionic biopolymer of high molecular weight composed of linear chains of β-D-mannuronic acid joined by type bonds (1 → 4) and residues of its epimer, α-L-guluronic acid. (biomedcentral.com)
  • In this study, the binding affinity and mechanism of human odor-binding protein OBP2a to 14 typical odorants in water were first assessed using fluorescent competitive binding assays and molecular docking techniques. (springeropen.com)
  • Molecular dynamic simulations have been done for top most active compound for validating its α-glucosidase and α-amylase inhibitory potential, RMSD analysis of ligand protein complex suggested the stability of top most active compound 5o in binding site of target proteins. (biomedcentral.com)
  • Silks are composed principally of proteins with a predominance of alanine, serine and glycine and silk proteins are able to undergo irreversible transformations from soluble protein to insoluble fibres. (bioone.org)
  • S contained approximately 10% carbohydrate and 15% protein and was mostly soluble in ethanol. (scirp.org)
  • Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)
  • Therefore, encapsulates containing vegetable oils showed promising results regarding the acceptability of sensorial aspects and the stability of fatty acids and phenolic compounds [ 11 ]. (biomedcentral.com)
  • It is affected by factors such as medium pH and by the presence of certain substances, for example cholesterol, other lipids, fatty acids, amines, amino acids, or proteins, incorporated in the lipid bilayer. (degruyter.com)
  • For fluorescence probing, the polarity sensitive nature of the tryptophan (Trp) residue is widely used. (azpdf.net)
  • Such wavelength selection targets the population of tryptophan residues, side chains of which are exposed to the polar aqueous environment. (edinst.com)
  • We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
  • We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)
  • Static electric field strengths of 0.001 V/nm and 0.002 V/nm induced conformational changes in the protein but had no significant effect on its surface properties. (mdpi.com)
  • The bioactive metabolites were screened using the phytochemical analysis, UV, FTIR and HPLC analysis. (rroij.com)
  • Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
  • In the following, we refer to pump of halophilic archaebacteria, has become the these as the extracellular (EC) channel and the paradigm of membrane proteins in general and ion cytoplasmic (CP) channel. (lu.se)
  • The chemical composition of the mycelium of M. androsaceus has been found to primarily contain mannitol, cholesteryl, amino acids, hydroxyl-cinnamic acid, ergosterol, carbohydrates, proteins and organic acids and sterols. (scirp.org)
  • They are a source of carbohydrates, proteins, and free amino acids, which may be used as substrates for the biosynthesis of various compounds, such as flavors, hormones, coenzymes, or neurotransmitters. (ncsu.edu)
  • Fucoidan found in Fucucvesicuclosus is a compound consists primarily of L-fucose and sulfate along with small quantities of D-galactose, D-manose, D-xylose and uronic acid. (rroij.com)
  • Microbial products such as enzymes, proteins, and bio-surfactants are the key to capping and/or stabilizing of the NPs in the biosynthesis process [ 4 ]. (springeropen.com)
  • The constituent proteins are normally synthesised in specialised glands where the epithelial cells are responsible for the biosynthesis. (bioone.org)
  • Furthermore, gel column chromatography on Sephadex G-150 indicated that the elution curves of the carbohydrate and protein overlapped significantly in sample P ( Figure 1 ). (scirp.org)
  • The mannose and glucose contents were also investigated, as well as the concentrations of proteins, free amino nitrogen (FAN), and free amino acids. (ncsu.edu)
  • Thermo Scientific Acclaro Intelligence Technology software (standard in our premier NanoDrop One/One C and NanoDrop Eight spectrophotometers) uses full-spectrum data and advanced algorithms to identify common nucleic acid contaminants and provide corrected nucleic acid concentrations. (thermofisher.com)
  • The application of enzymes for waste treatment has been gaining pace commercially to solve concerns related to agricultural residues, wastewater, replacement of synthetic processes with natural ones, etc. (preprints.org)
  • Fe-S clusters are present in more than 300 different types of enzymes or proteins and play crucial roles in fundamental life processes such as respiration, photosynthesis and nitrogen fixation. (uga.edu)
  • The advantages of this nucleic acid quantitation method are that it is simple, direct, and consumes only a small volume of your sample. (thermofisher.com)
  • Microbial rhodopsins (Rhs) are transmembrane proteins that utilize light-induced isomerization of their retinal cofactor to function as light-sensitive ion channels, pumps and sensors. (elifesciences.org)
  • The gum contains alternating 4- O- substituted and 2- O -substituted α d -mannopyranose units and chains of 1 → 6 linked β d -galactopyranose units with side chains of l -arabinofuranose residues. (springeropen.com)
  • The self-association property of Nab3 adds to the previously documented interactions between these hnRNP-like proteins, RNA polymerase II, and the nascent transcript, Selleck Entinostat leading to a network of nucleoprotein interactions that define a higher order Nrd1-Nab3 complex. (chksignal.com)
  • Collagen, the most abundant protein in the animal kingdom, represents a promising biomaterial for regenerative medicine applications due to its structural diversity and self-assembling complexity. (biomedcentral.com)
  • Collagen is a fibrous protein found in all multicellular animals (Voet et al. (earthwormexpress.com)
  • The amino acid sequence in collagen is generally a repetitive tripeptide unit (Gly-X-Y), where X is frequently Pro and Y is Hyp (Nelson and Cox, 2004). (earthwormexpress.com)
  • Gelatin is a protein resulting from the controlled hydrolysis of collagen from different animal sources (porcine, bovine, and fish), with applications in the food, pharmaceutical, and cosmetic industries. (biomedcentral.com)
  • volume = {285}, number = {32}, pages = {24307--24312}, abstract = {Inhibitory serpins are metastable proteins that undergo a substantial conformational rearrangement to covalently trap target peptidases. (cnrs.fr)
  • However, the organic acids and sterols in the mycelium of the fungus are very low in quantity and are water-insoluble. (scirp.org)
  • Amyloids are insoluble fibrous protein aggregations sharing specific structural traits. (wikidoc.org)
  • Here, we present a UV-Vis and FTIR study about the light-induced dynamics of a RGC from the fungus Catenaria anguillulae , which provides insights into the catalytic process. (elifesciences.org)
  • Cornish and co-workers could show that amino-acid substitutions along the putative PT pathway dramatically decreased the catalytic activities. (nature.com)
  • HAP nanoparticles have been used as vehicles for delivery due to their affinity to DNA, proteins, several drugs, and proper release activity. (encyclopedia.pub)
  • The cDNA encoding P2 was cloned into pPROEXHTa vector which itself codes Cyanoginosin-LR structure for a thirty residue affinity tag including 6 histidines. (dna-alkylating.com)
  • The protein was affinity purified on Ni-NTA beads (Sigma-Aldrich) and eluted with Tris buffer (pH seven.5 twenty mM) that contains NaCl (one hundred fifty mM), imidazole (250 mM) and DTT (5 mM). (dna-alkylating.com)
  • The intensity of their maximum absorbance peak at 260 nm is proportional to nucleic acid concentration. (thermofisher.com)
  • Significant differences between fluorescence lifetimes of "free" Trp derivatives hydroxytryptophan (OH-Trp), N-formylkynurenine (NFK), kynurenine (Kyn), hydroxykynurenine (OH-Kyn) and their residues were observed. (edinst.com)
  • 100 references, on organotin(IV) complexes of the carboxylic acid derivatives are presented with special reference to their methods of synthesis, spectroscopic and structural studies and their biological activities. (degruyter.com)
  • Green fluorescence protein (GFP) is a protein that emits green fluorescence when exposed to ultraviolet light and has been shown to have chiroptical properties 1 . (jascoinc.com)
  • We show that the cumulative fraction of OH-Trp, NFK and ArgP emission dominates the total fluorescence spectrum in both emulsified post-surgical human cataract protein samples, as well as in whole lenses and that this correlates strongly with cataract grade and age. (edinst.com)
  • These absorbance measurements allow scientists to measure nucleic acid concentration and have an indication of sample purity. (thermofisher.com)
  • they are stabilized by hydrogen bonds and intermolecular bonds (Silva and Penna, 2012), which are composed of three helical polypeptide chains, each with about 1000 amino acids, which are called an α chain. (earthwormexpress.com)
  • Manipulation of proteins by chemical modification is a powerful way to decipher their function or harness that function for therapeutic purposes. (biorxiv.org)
  • Chemical or genetic engineering of proteins provides great potential to study protein function and pharmacology or to generate proteins with novel properties. (biorxiv.org)
  • However, despite recent technical achievements 1 , 2 , the type of chemical modification that can be accomplished by genetic means (e.g. amber codon suppression) is limited to incorporation of non-canonical amino acids (ncAAs) due to the tolerance of the cell's translational machinery. (biorxiv.org)
  • This assembly results in a spontaneous, essentially traceless splicing reaction that covalently links the two flanking protein segments through native chemical ligation 9 . (biorxiv.org)
  • The Metschnikowia lysates showed different chemical profiles and the pool of individual amino acids was generally smaller. (ncsu.edu)
  • With advances in UV-Vis software, researchers can now selectively quantify nucleic acid absorbance in the presence of chemical and nucleic acid contaminants . (thermofisher.com)
  • Gluten is a storage protein found in wheat, it plays an important role in regulating its baking quality by determining the viscosity, cohesivity, water absorption and elasticity of the dough [ 1 ]. (mdpi.com)
  • We also collaborate with other groups for Mossbauer, electron-nuclear double resonance (ENDOR), X-ray absorption and X-ray crystallography studies of proteins prepared in our laboratory. (uga.edu)
  • 2020. Protein hydrolysates from Alphitobius diaperinus and Hermetia illucens larvae treated with commercial proteases. (eaap.org)
  • We propose that the pH-dependent changes of the protonation states of glutamate/aspartate residues of APRs initiate switching between the amyloid and the folded, monomeric forms of the hormones. (nature.com)
  • The results from the mutations at these four residues show that the energies of PL or PM can be preferentially changed depending on the placement of a charged residue. (trpv-antagonist.com)
  • In the 9 M urea denatured condition the protein displays primarily non-native b structural propensity. (dna-alkylating.com)
  • Jiri Jonas is considered a pioneer in the use of magnetic resonance imaging at high pressure, developing techniques to study the dynamic structure of liquids and proteins. (wikipedia.org)
  • This approach has been used in the study of the arc repressor, a DNA-binding protein containing 53 amino acid residues. (wikipedia.org)
  • The amount of B-side electron transfer after excitation at 390 nm has been observed to be altered in the HE(L168)/ND(L170) mutant in a pH-dependent manner that has been interpreted as arising from the presence of ionizable amino acids residues (Haffa et al. (trpv-antagonist.com)
  • Since Lescher and col. prepared the nalidixic acid in 1962 [ 1 ], the synthesis of new quinolones continues (Figure 1 ). (hindawi.com)
  • In the present work we reported the synthesis, characterization, and biological activity results of a copper mixed complex (see Figure 1 ), with hsm and nalidixic acid. (hindawi.com)
  • While the locations and inferred H-bond connec- water molecules in a membrane protein, despite tivities of these internal water molecules are essen- being deeply buried and extensively H-bonded to tial, one would also like to quantify their mobility. (lu.se)
  • Biopolymers, such as proteins, DNA, and polysaccharides, which are available in almost unlimited quantity in nature, as well as many synthetic polymers, which are industrially produced on a large scale, belong to this fascinating class of polymers. (mdpi.com)
  • Semi-synthetic approaches offer an alternative means to manipulate proteins post-translationally, but these modifications have typically been performed in vitro 3 - 8 . (biorxiv.org)
  • The results of these studies suggest that a higher superficial surface area leads to higher protein adsorption, which is also reported in other studies [ 10 ] . (encyclopedia.pub)
  • And they are so for a very long time: there are studies out there that looked at 195 million year old proteins. (picamed.com)
  • The macro algae phaeophyceae (scytosiphongracilis) have high quantity of protein contents and was used in proteomic studies [ 7 ]. (rroij.com)
  • Our herein reported results can be of help to elucidate differences in self-assembly states of proteins using ITC to improve the design of energy responsive and dynamic materials for applications in tissue engineering and regenerative medicine. (biomedcentral.com)
  • The long-term objective of our work on biochemical systems is to contribute to the understanding of folding of proteins, folding intermediates and structure of pressure denatured states. (wikipedia.org)
  • This toxicity is exerted, at least in part, through the generation of reactive oxygen species (ROS), which is well-known to induce oxidative stress in the cell that damages important macromolecules such as proteins, DNA, and membranes [ 14 ]. (biomedcentral.com)
  • where they found that HAP nanopowders containing 100 nm particles adsorbed more proteins than 1 µm [ 9 ] . (encyclopedia.pub)
  • Copper in biological systems is usually found surrounded by imidazole moieties from histidine residues [ 13 , 14 ]. (hindawi.com)
  • The solubilized protein was found to be stable in the dark for at least eight months at 277 K. (lu.se)
  • Iron-sulfur protein maturation in Helicobacter pylori:Identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets. (uga.edu)
  • ERY, marked as a risk prioritized macrolide antibiotic by 2015 released European Union watch list, most probably due to its protein inhibition capability considered third most widely used antibiotic. (afpm.org.my)
  • The method is called palaeoproteomics and focuses on the proteins in ancient to very ancient biological material. (picamed.com)
  • Moreover, the first organocatalyzed Mukaiyama-Michael vinylogous reaction of trimethylsilyloxyfuran to nitroalkenes is described.The reaction is promoted by Brønsted acids under solvent-free conditions, taking place in moderate to good yield with variously substituted nitroalkenes. (degruyter.com)
  • The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)