• Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4A-I and eIF4A-II, two other members of the DEAD box protein family. (wikipedia.org)
  • Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)
  • spMex67p, a 596-amino-acid-long protein, has considerable sequence similarity to the Saccharomyces cerevisiae Mex67p (scMex67p) and human Tap. (ox.ac.uk)
  • The RCC1 gene, a regulator for the onset of chromosome condensation was found to encode a protein with a molecular mass of 45 kD, determined using the antibody against the synthetic peptides prepared according to the amino acid sequence of the putative RCC1 protein. (silverchair.com)
  • A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)
  • A codon is a DNA or RNA sequence of three nucleotides (a trinucleotide) that forms a unit of genetic information encoding a particular amino acid. (genome.gov)
  • The glycolate cycle is thought to bring about the formation of the amino acids-glycine and serine-from the non-phosphorylated intermediates of photosynthetic carbon reduction cycle, i.e., glycerate to serine, or glycolate to glycine and serine in a sequence of reactions which involve chloroplasts, peroxisomes, mitochondria and cytosol. (exama2z.in)
  • Using pattern searches and position-dependent matrices, we have extracted the AT-hook motifs present in a non-redundant protein sequence database. (embl.de)
  • We have classified these motifs into three types according to their sequence similarity and have found that they are prevalent in many eukaryotic nuclear proteins in single or multiple copies. (embl.de)
  • The HMG family of proteins comprises members with multiple HMG domains that bind DNA with low sequence specificity, and members with single HMG domains that recognize specific nucleotide sequences. (embl.de)
  • The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
  • The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)
  • PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2). (novusbio.com)
  • Moreover, speci®c hy- found in narrow minor groove regions in a variety dration patterns seem to play a role in nucleotide sequence recognition by proteins (Otwinowski of oligonucleotide duplexes (Prive et al. (lu.se)
  • Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. (nih.gov)
  • Thus, RCC1 protein locates on the chromatin and is not a component of the nuclear matrix. (silverchair.com)
  • HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. (embl.de)
  • Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)
  • The experiments show that GFP-Foxg1 mobile molecules have a chromatin affinity that decreases in truncated mutants with the extension of the protein deletion. (sns.it)
  • Modified FANCD2 interacts with BRCA2 PROTEIN in a stable complex with CHROMATIN, and it is involved in DNA REPAIR by homologous RECOMBINATION. (bvsalud.org)
  • In contrast to scMEX67, spmex67 is essential for neither growth nor nuclear export of mRNA. (ox.ac.uk)
  • We identified a central region (149 to 505 amino acids) within spMex67p that associates with a complex containing Rae1p that complements growth and mRNA export defects of the rae1-167 Deltamex67 synthetic lethality. (ox.ac.uk)
  • Overexpression of spMex67p inhibits growth and mRNA export and results in the redistribution of the diffused localization of the (149-505)-GFP fusion to the nucleus and the nuclear periphery. (ox.ac.uk)
  • Presumably, RCC1 protein plays a role in regulating the onset of chromosome condensation, at the level of transcription or of mRNA maturation. (silverchair.com)
  • Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
  • In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein. (genome.gov)
  • These radicals may act on DNA molecule to produce mutations altering the transcription into mRNA and the translation into proteins. (exama2z.in)
  • In addition, free radicals and peroxides can affect the membranes by causing peroxidation of lipids and proteins. (exama2z.in)
  • This course will cover the structure and function of different biological molecules, including proteins, carbohydrates, lipids and nucleic acids. (uaeu.ac.ae)
  • Existing studies have confirmed that ferroptosis involves the metabolisms of the Fe, lipids, amino acid, each mechanism is mutually independent but interrelated, and they are formed a complex regulatory network. (frontiersin.org)
  • The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. (nih.gov)
  • Less active sections are placed near the nuclear lamina, which is close to membrane. (jonlieffmd.com)
  • They are bounded by a single membrane and have an interior or matrix which is amorphous or granular Micrbodies are most easily distinguished from other cell organelles by their content of catalase enzyme. (exama2z.in)
  • They have a single limiting unit membrane of lipid and protein molecules, which encloses their granular matrix. (exama2z.in)
  • The latter are transported and sorted into the inner membrane and matrix compartments of the mitochondria ( Neupert & Herrmann, 2007 ). (life-science-alliance.org)
  • Cox6, on the other hand, is a subunit of yeast COX that is peripherally bound to this complex on the matrix side of the inner membrane ( Rathore et al, 2019 ). (life-science-alliance.org)
  • Analysis of the blue coral transcriptome revealed enrichment of genes involved in stress response, including heat-shock proteins and antioxidants, as well as genes participating in signal transduction and stimulus response. (nature.com)
  • Rather, it is way that genes are utilized differently in each type of cell that determines which proteins will produce unique structures. (jonlieffmd.com)
  • In fact, recent research ( ENCODE) shows that pieces of DNA can be taken from multiple different "genes" to make one protein, making the entire definition of gene suspect. (jonlieffmd.com)
  • If less than 2% are so-called "genes" and 40% of the RNA is useful, then the regulatory RNA is 20 times more than those that code for proteins. (jonlieffmd.com)
  • COX, responsible for the reduction of oxygen to water, consists of three catalytic core subunits (Cox1, Cox2, and Cox3), each derived from a mitochondrial gene and of 8-10 additional structural subunits that are expressed from nuclear genes. (life-science-alliance.org)
  • A transcriptional co-repressor is a protein that interacts with other DNA-binding proteins to suppress the activity of certain genes, although it cannot attach (bind) to DNA by itself. (medlineplus.gov)
  • These include 1) signaling events, 2) the transcriptional and post-transcriptional regulation of nuclear-encoded genes that regulate mitochondrial turnover, 3) import of proteins into the organelles, 4) mitochondrially-mediated apoptosis, 5) the regulation of mitochondrial morphology via fission and fusion, and 6) mitophagy, as mediated by lysosomal activity. (yorku.ca)
  • The glycolate pathway also generates C 1 compounds and serves as the generator of precursors for nucleic acid biosynthesis. (exama2z.in)
  • of nucleic acid structure (Westhof, 1988). (lu.se)
  • Further investigation led me to discover that Foxg1 undergoes a proteolytic cleavage and the resulting C-terminal peptide localizes to mitochondria, as evidenced by CFP-Foxg1 wt-YFP fusion protein as well as by other biochemical and immunohistochemical assays. (sns.it)
  • Considering that Foxg1 does not have a classical N terminal mitochondrial targeting signal, I looked for Foxg1 amino acids able to drive a fused fluorescent tag into mitochondria and identified amino acids 277-302 as critical. (sns.it)
  • To determine if Atco is also a precursor of COX, we pulse-labeled Cox6 in isolated mitochondria of a cox6 nuclear mutant with COX6 in mitochondrial DNA. (life-science-alliance.org)
  • (C) Newly expressed nuclear-derived proteins are then imported into mitochondria through translocases of the outer, and then inner membranes (TOM and TIM, respectively). (yorku.ca)
  • (D) The exercise stimulus increases protein synthesis within mitochondria, which promotes the activation of the UPRmt. (yorku.ca)
  • A pull down experiment showed that a 'mitochondrial' Foxg1 peptide interacts with some proteins involved in mitochondrial trafficking and function such as voltage dependent anion channel 1 and 2. (sns.it)
  • Normal human peripheral blood cells were stimulated overnight with Human IL-2 Recombinant Protein (Product # 14-8029-81), Anti-Human CD3, and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81). (thermofisher.com)
  • Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 produced in E.coli. (thermofisher.com)
  • The lamin family of proteins make up the matrix and are highly conserved in evolution. (nih.gov)
  • Since we proposed the concept of a matrix degrading phenotype in TB a decade ago, diverse data implicating MMPs as key mediators in TB pathology have accumulated. (ersjournals.com)
  • Conversely, the immobile fraction does not decrease gradually with the extension of protein deletion but is significantly higher for a truncation associated with a severe phenotype, suggesting a possible dominant negative function of some RTT mutants. (sns.it)
  • Special protein structures alter polymerase activity. (jonlieffmd.com)
  • It is a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target protein. (biosensis.com)
  • Explain The Various Ways That Enzymes Can Be Regulated, Including Post-Translational Modifications Of Proteins And Their Degradation. (uaeu.ac.ae)
  • Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
  • Proteins that do not bind the receptor are directed to lysosomal degradation. (nature.com)
  • DNA strands are wound on spools of histone protein molecules and then molded into a variety of structures. (jonlieffmd.com)
  • For each type of process, specific large protein molecules aid the formation of 3D structures. (jonlieffmd.com)
  • Usually, the NOE and NMRD in B-DNA is expected to be less ``integral'' than methods can only provide bounds on the residence water molecules buried inside proteins. (lu.se)
  • The extended CAG region changes the structure of atrophin 1 and how the protein interacts with other proteins to control gene function. (medlineplus.gov)
  • Krüppel-like factors (KLFs) are highly conserved zinc-finger proteins that regulate cellular transcription machinery [ 1 , 2 ]. (medsci.org)
  • Recent studies have focused on asymmetric synthesis of structurally complex amino acids, using what has proved to be a very versatile radical-based methodology and also the use of structurally unusual heterocycles for masking the reactivity of synthetically valuable functional groups. (exeter.ac.uk)
  • The use of radical-based methodology in the synthesis of highly substituted amines and amino acids. (exeter.ac.uk)
  • The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. (novusbio.com)
  • The AT-hook is a small DNA-binding protein motif which was first described in the high mobility group non-histone chromosomal protein HMG-I(Y). Since its discovery, this motif has been observed in other DNA-binding proteins from a wide range of organisms. (embl.de)
  • The 1st identified mobile receptor because of this nuclear proteins was the receptor for advanced glycation end items (Trend), which mediates the relationships between advanced glycation end item (Age group)Cmodified proteins as well as the endothelium and additional cell types (7). (exposed-skin-care.net)
  • Its differentiation and function are mainly regulated by macrophage colony-stimulating factor (M- CSF), receptor activator of nuclear factor kappa B(RANK) ( 7 ). (frontiersin.org)
  • RANK binds to transmembrane protein nuclear factor-κB receptor activating factor ligand (RANKL) to activate RANK/RANKL, TRAF6/RANKL/MAPKs, TRAF6/ RANKL/NF-κB, Wnt/RANKL/RANKL, and JAK2/STAT3/RANKL. (frontiersin.org)
  • Several other mutations associated with Paget disease have been identified, many affecting the RANK (receptor activator of nuclear factor kappa-B) signaling pathway that is critical for osteoclast generation and activity. (msdmanuals.com)
  • It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells. (thermofisher.com)
  • Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. (thermofisher.com)
  • Hence, these proteins could be candidate antigen targets for the development of vaccines aimed at the control and prevention of O. erraticus infestations and the diseases this tick transmits. (biomedcentral.com)
  • Environmental sequences of ten novel plastid lineages and structural innovations in plastid proteins confirm that plastids in apicomplexans and their relatives are widespread and share a common, photosynthetic origin. (elifesciences.org)
  • Eukaryotic initiation factor 4A-III is a protein that in humans is encoded by the EIF4A3 gene. (wikipedia.org)
  • Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. (thermofisher.com)
  • Mutagenesis of the nucleocapsid protein of Nipah virus involved in capsid assembly. (cdc.gov)
  • Mapping of domains responsible for nucleocapsid protein-phosphoprotein interaction of Henipaviruses. (cdc.gov)
  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. (wikipedia.org)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and binds to DNA. (silverchair.com)
  • To fit 2 yards of DNA into a tiny nucleus is a monumental engineering feat. DNA is highly compacted yet has to be instantly available to rapidly make proteins in neurons with a momentary change of thought. (jonlieffmd.com)
  • Speaking more specifically, β-catenin is an integral structural component of cadherin-based adhesive junctions as well as it a key nuclear effector of canonical in nucleus Wnt signaling. (chemdiv.com)
  • The cleavage of terminally misfolded protein aggregates in the matrix and the release of their peptide fragments, block ATF5 mitochondrial entry, thus redirecting it to the nucleus to upregulate transcription of mitochondrial chaperones and proteases and equip the organelle with an augmented capacity for protein folding. (yorku.ca)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • Further, there are signaling pathways that participate in regulation, such as Wnt channel, mitogen-activated protein kinase (MAPK) channel, bone morphogenetic protein (BMPs) channel, peroxisome proliferator activated-receptors (PPARs) channel, and Notch channel. (frontiersin.org)
  • Although the exact function of this protein is unknown, it appears to play an important role in nerve cells (neurons) in many areas of the brain. (medlineplus.gov)
  • This altered protein accumulates in neurons and interferes with normal cell functions. (medlineplus.gov)
  • Macrophage inflammatory protein-2: chromosomal regulation in rat intestinal epithelial cells. (qmul.ac.uk)
  • It is most interesting that this motif seems to be quite specific to known or predicted chromosomal/DNA-binding proteins, suggesting that it may act as a versatile minor groove tether. (embl.de)
  • Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)
  • An amino acid is the fundamental molecule that serves as the building block for proteins. (genome.gov)
  • Tripan Blue and proteinase K protection experiments in living cells show that nearly 80% of the protein reside in the mitochondrial matrix. (sns.it)
  • Unfortunately, protein and some elements in existing dispersion media are suboptimal for potential nose-to-brain transport of nanomaterials because olfactory transport has size- and ion-composition requirements. (cdc.gov)
  • Therefore, we designed a protein-free dispersion media containing phospholipids and amino acids in an isotonic balanced electrolyte solution, a solution for nasal and olfactory transport (SNOT). (cdc.gov)
  • Lamin B2 contributes to the proliferation of bladder cancer cells via activating the expression of cell division cycle‑associated protein 3. (nih.gov)
  • OA onset results in the upregulation of several inflammatory cytokines including interleukin 1 (IL-1) and tumor necrosis factor-alpha (TNF-α) which in turn upregulate the expression of proteolytic enzymes that break down the cartilage matrix [ 3 , 4 ]. (biomedcentral.com)
  • Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. (thermofisher.com)
  • Many of these proteins are regulators of gene expression. (embl.de)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • Zymography assay demonstrated that KLF6 inhibited the activities of matrix metalloproteinase 9 (MMP-9) and weakened the expression of mesenchymal markers, such as snail, slug, and vimentin. (medsci.org)
  • Dysregulated expression of lysyl oxidase-like 2 (LOXL2), matrix metalloproteinase 9 (MMP9) and lipocalin 2 (LCN2) are associated with ECM remodelling. (bvsalud.org)
  • New insights into the role of sequestosome 1/p62 mutant proteins in the pathogenesis of Paget's disease of bone. (msdmanuals.com)
  • Taken together, these results suggest the following hypothesis: Foxg1 is a nuclear and mitochondrial protein that upon its internal processing localizes in the mitochondrial compartment and becomes a key coordination part of the machinery that brings about cell differentiation, replication and bioenergetics. (sns.it)
  • (E) Mitochondrial fusion proteins Mfn1/2 along with Opa1 facilitate the fusion of the outer and inner membranes, respectively, allowing for improved sharing of metabolites amongst neighbouring organelles. (yorku.ca)
  • Nitrogen is also present in amino acids, proteins, and nucleic acids, which play important roles in the human body. (sinap.ac.cn)
  • Draw And Explain The Reactions Involved In Photosynthesis And The Nitrogen Cycle Including Amino Acid Anabolism. (uaeu.ac.ae)
  • The aim of these studies has been to develop a much-needed general synthetic route to amino acids (and other amine derivatives) possessing a quaternary chiral centre alpha -to the nitrogen atom and in particular, alpha , alpha -disubstituted alpha -amino acids. (exeter.ac.uk)
  • Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)
  • In this study, protein-protein interaction assays indicated that LCN2 and LOXL2 interactions and LCN2 and MMP9 interactions occurred both intracellularly and extracellularly, but interactions between LOXL2 and MMP9 only occurred intracellularly. (bvsalud.org)
  • During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. (nih.gov)
  • Product Description google Mouse anti-Ectoderm-heural cortex protein 1 (ENC-1) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA. (biosensis.com)
  • The aim of this course is to introduce basic biochemical laboratory techniques, with emphasis on protein biochemistry. (uaeu.ac.ae)
  • WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif. (embl.de)
  • In general, it appears that the AT-hook motif is an auxiliary protein motif cooperating with other DNA-binding activities and facilitating changes in the structure of the DNA either as a polypeptide on its own [e.g. (embl.de)
  • ENC-1 is an actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. (biosensis.com)
  • Although the structural subunits do not participate directly in either the reduction of oxygen or transfer of protons to the matrix, with a few exceptions, they are essential for assembly and stability of COX ( Tzagoloff & Dieckmann, 1990 ). (life-science-alliance.org)
  • Additionally, the dense avascular matrix of cartilage is made up of a network of collagen II enmeshed with densely packed aggrecans comprising a high density of negatively charged sulfated glycosaminoglycans (GAGs), which hinders the passive diffusion of drugs in cartilage [ 5 ]. (biomedcentral.com)
  • The major antibody class found in the blood, IgG, has a remarkable persistence, with a serum half-life of 20-23 days, compared with only hours or a few days for other circulating proteins 5 . (nature.com)
  • Quantified proteins were classified into 23 functional categories and their abundance compared between sexes. (biomedcentral.com)
  • They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. (wikipedia.org)
  • This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)
  • This region is devoid of RNA-binding, N-terminal nuclear localization, and the C-terminal nuclear pore complex-targeting regions. (ox.ac.uk)
  • Increasingly, these "epigenetic" mechanisms (that is, mechanisms outside of the simple procedure of assigning an amino acid directly from a code in a gene) are being found to be vastly more complex than ever imagined. (jonlieffmd.com)
  • It has been known for some time that the shape of proteins determines their function and the folding is very complex involving four levels of folding (see post Protein Shape Determines Function ). (jonlieffmd.com)
  • In yeast, this bridging involves interactions between the WW domains in the splicing factor PRP40 and a proline-rich domain in the branchpoint binding protein, BBP. (embl.de)
  • High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. (embl.de)