• Additionally, BRDs contain several catalytic domains that enable them to act as methyltransferases, ATP-dependent re-modellers or histone acetyltransferases and helicases ( 8 ) Bromodomain and extra-terminal domain (BET) proteins are a family of transcriptional mediators that regulate gene expression ( 8 , 9 ). (frontiersin.org)
  • Post-translational modification of proteins at lysine residues by reversible acetylation is catalyzed by the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs), which act on both histone and non-histone substrates despite their names. (biomedcentral.com)
  • A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. (bvsalud.org)
  • Other names in common use include beta-endorphin acetyltransferase, peptide acetyltransferase, protein N-terminal acetyltransferase, NAT, Nalpha-acetyltransferase, amino-terminal amino acid-acetylating enzyme, and acetyl-CoA:peptide alpha-N-acetyltransferase. (wikipedia.org)
  • Here we report that nonglycosylated full-length and C-terminal truncated amyloid precursor protein (APP) accumulates exclusively in the protein import channels of mitochondria of human AD brains but not in age-matched controls. (jneurosci.org)
  • The major constituent of a senile plaque is β-amyloid (Αβ), which is a 40-43 amino acid peptide produced by the action of secretory pathway-associated proteases, namely β and γ secretases, at the C terminus of a type I membrane-spanning glycoprotein termed amyloid precursor protein (APP). (jneurosci.org)
  • The substrate specificities of different NAT enzymes are mainly determined by the identities of the first two N-terminal residues of the target protein. (hmdb.ca)
  • Protein N-terminal acetylation is among the most common types of protein modifications occurring on approximately 50% of all yeast proteins and more than 80 % of all human proteins. (biocat.no)
  • Surprisingly, there is yet no clear functional understanding of how N-terminal acetylation affects proteins in general, although recent data indicate roles in protein degradation and targeting. (biocat.no)
  • An N-terminal acetyltransferase (NAT) transfers an Acetyl group from Acetyl Coenzyme A to the alpha-amino group of the Nterminal amino acid residue of the protein. (biocat.no)
  • The NUP98/KDM5A fusion protein contains the Phe-Gly (FG) repeats of the N-terminal part of NUP98. (atlasgeneticsoncology.org)
  • Fatty acid synthase is a multifunctional protein. (thermofisher.com)
  • From the identified 2938 proteins and 7503 unique peptides, we characterized N-terminal methionine excision, co- and posttranslational Nα acetylation, protein maturation, and proteolytic processing of proteins in human platelets. (ashpublications.org)
  • N-acetyl amino acids can be produced either via direct synthesis of specific N-acetyltransferases or via the proteolytic degradation of N-acetylated proteins by specific hydrolases. (hmdb.ca)
  • 21750686 ). Several proteins from prokaryotes and archaea are also modified by N-terminal acetylation. (hmdb.ca)
  • Bromodomains are proteins that contain modules of ~110 amino acids that recognize and bind acetylated lysine residues in histones and other proteins. (frontiersin.org)
  • Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. (embl-heidelberg.de)
  • This position of the promoter permits positive selection and attenuated expression of fusion proteins with C-terminal tags. (biomedcentral.com)
  • Changes in levels or in the amino acid sequence of aSyn (by duplication/triplication of the aSyn gene or point mutations in the encoding region) cause familial cases of synucleinopathies. (bvsalud.org)
  • The KDM5A (JARID1A) sequence starting with exon 28 still contains the sequence encoding the C-terminal PHD domain. (atlasgeneticsoncology.org)
  • Purification and N-terminal amino acid sequence of the Ah receptor from the C57BL/6J mouse. (aspetjournals.org)
  • The hallmark of the family is a domain of approximately 260 amino acids that has a high degree of sequence similarity in all sirtuins. (biomedcentral.com)
  • Methylation of different part of the N-terminal of H3 and H4 histones is linked with both repressed and active chromatin, depends on the particular amino acid that is modified in the histone tail. (microbiologynotes.org)
  • Sulphoacetaldehyde acetyltransferase yields acetyl phosphate: purification from Alcaligenes defragrans and gene clusters in taurine degradation. (rhea-db.org)
  • The K-histone acetyltransferase KAT3B (p300) could play a role. (cancer-research-network.com)
  • In addition, CPTH2 decreases the invasiveness of a clear cell renal carcinoma (ccRCC) cell line through the inhibition of acetyltransferase p300 (KAT3B). (cancer-research-network.com)
  • Results in deregulation of HOXA genes through recruitment of the histone acetyltransferase CBP / p300 (Thiollier et al. (atlasgeneticsoncology.org)
  • CDK8 can directly interact with Mad in vitro through the linker region between the DNA-binding MH1 (Mad homology 1) domain and the carboxy terminal MH2 (Mad homology 2) transactivation domain. (sdbonline.org)
  • In addition to the catalytic domain, eukaryotic sirtuins contain variable amino- and carboxy-terminal extensions that regulate their subcellular localizations and catalytic activity. (biomedcentral.com)
  • the molecular mass of the subunit was found to be between about 63 kDa (SDS/PAGE) and 65.3 kDa (matrix-assisted laser-desorption ionization-time-of-flight MS). The N-terminal and two internal amino acid sequences were determined, and PCR primers were generated. (rhea-db.org)
  • Structure and ligand of a histone acetyltransferase bromodomain. (embl-heidelberg.de)
  • The nature of the recognition of acetyl-lysine by the P/CAF bromodomain is similar to that of acetyl-CoA by histone acetyltransferase. (embl-heidelberg.de)
  • In enzymology, a peptide alpha-N-acetyltransferase (EC 2.3.1.88) is an enzyme that catalyzes the chemical reaction acetyl-CoA + peptide ⇌ {\displaystyle \rightleftharpoons } Nalpha-acetylpeptide + CoA Thus, the two substrates of this enzyme are acetyl-CoA and peptide, whereas its two products are Nalpha-acetylpeptide and CoA. (wikipedia.org)
  • The systematic name of this enzyme class is acetyl-CoA:peptide Nalpha-acetyltransferase. (wikipedia.org)
  • A synthesized peptide derived from human FASN, corresponding to a region within N-terminal amino acids. (thermofisher.com)
  • 3473611 ). N-Acetyl-L-phenylalanine is a product of enzyme phenylalanine N-acetyltransferase [EC 2.3.1.53] Which is found in the phenylalanine metabolism pathway. (np-mrd.org)
  • Amino acid metabolism.biosynthesis.glutamate. (ntu.edu.sg)
  • Intracellular metabolism of 5,10-dideazatetrahydrofolic acid in human leukemia cell lines. (aspetjournals.org)
  • 30054468 ). NatA also exists in a monomeric state and can post-translationally acetylate acidic N-termini residues (D-, E-). NatB and NatC acetylate N-terminal methionine with further specificity determined by the identity of the second amino acid. (hmdb.ca)
  • In humans as in yeast, three NAT complexes NatA, NatB and NatC are believed to perform most N-terminal acetylations. (biocat.no)
  • 16 this range (primarily cytochrome P-450 mediated oxidation to fatty acids and alcohols) is slow, while the aromatics are metabolized faster (oxidation of alkyl site and/or ring, sometimes with formation of reactive intermediates, and conjugation with glutathione, glucuronic acid, or glycine) (ATSDR 1999). (cdc.gov)
  • 16465618 ). Aminoacylase I is a soluble homodimeric zinc binding enzyme that catalyzes the formation of free aliphatic amino acids from N-acetylated precursors. (hmdb.ca)
  • A newly developed β-lactamase (ΔW290) selection cassette contains a segment inside the β-lactamase open reading frame encoding a stretch of hydrophilic amino acids that result in a T7 promoter when back-translated. (biomedcentral.com)
  • We used a dedicated N-terminomics technique, iTRAQ terminal amine isotopic labeling of substrates (TAILS), to characterize the human platelet N-terminome, proteome, and posttranslational modifications throughout platelet storage over 9 days under blood-banking conditions. (ashpublications.org)
  • They occur at any stage in the "life cycle" of histone primarily on the flexible N-terminal tails protruding from the surface of the nucleosome as well as on the globular core region, and they are most often mediated by enzymatic activity. (creativebiomart.net)
  • Histone methylation is the introduction of methyl groups to certain amino acids in a histone and it plays a critical role in the epigenetic regulation of gene transcription and may serve as a global epigenetic mark. (creativebiomart.net)
  • Quantification of acetylated histone would provide meaningful information for further characterizing the acetylation patterns or sites, and thereby cause a better understanding about epigenetic regulation of gene activation, and exploration of drugs targeting histone acetyltransferase (HAT) or histone deacetylase (HDAC). (creativebiomart.net)
  • Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. (thermofisher.com)
  • In this study, using domain structure and amino acid alignment and phylogenetic tree construction, rimR 2, which located in the rimocidin biosynthetic gene cluster, was first found and identified as a larger ATP-binding regulators of the LuxR family (LAL) subfamily regulator. (biomedcentral.com)
  • Using a human cortical neuronal (HCN) culture system, we showed that full-length APP is targeted to both mitochondria and plasma membrane (PM) by virtue of N-terminal chimeric signals. (jneurosci.org)
  • calcium inflow releases neurotransmitter molecules from many vesicles by fusing the vesicle membranes to the nerve terminal membrane. (msdmanuals.com)
  • Interestingly, mitochondrial translocation of APP was incomplete because of an internal acidic domain present between 220 and 290 amino acids. (jneurosci.org)
  • tRNA synthetase class II core domain (G, Seryl-tRNA synthetase N-terminal domain [Interproscan]. (ntu.edu.sg)
  • Molydopterin dinucleotide binding domain, Molybdopterin oxidoreductase N-terminal domain [Interproscan]. (ntu.edu.sg)
  • Gives feature of a main globular domain and have an extended N-terminal tail. (microbiologynotes.org)
  • Through these studies, a complex and specific system of N-terminal acetylation has been revealed. (biocat.no)
  • Technically, N-Acetylglutamine is a biologically available N-terminal capped form of the proteinogenic alpha amino acid L-glutamine. (hmdb.ca)
  • The facultatively anaerobic bacterium Alcaligenes defragrans NKNTAU was found to oxidize taurine (2-aminoethanesulphonate) with nitrate as the terminal electron acceptor. (rhea-db.org)
  • The downstream gene presumably encoded the inducible phosphate acetyltransferase (Pta) found in crude extracts. (rhea-db.org)
  • Impulse transmission is chemical, caused by release of specific neurotransmitters from the nerve ending (terminal). (msdmanuals.com)
  • The c- Jun and c-Fos binding sites were localized to the C-terminal subregion of SRC-1 (amino acids 1101-1441) that encompasses the previously described histone acetyltransferase and receptor-binding domains. (elsevierpure.com)
  • They are activated by proteolytic cleavage of the N-terminal region in the extracellular compartment. (medscape.com)
  • Variable tags are available both in N-terminal or C-terminal position. (biomedcentral.com)
  • Further, the expression of phosphinothricin N-acetyltransferase also allowed for selection of modified plants during transformation. (cbd.int)
  • For axenic growth under laboratory conditions, it specifically relies on the external supply of N -acetylmuramic acid (MurNAc), which is an essential constituent of the peptidoglycan (PGN) of bacterial cell walls. (karger.com)
  • N-terminal acetylation is a co-translational process occurring on the ribosomes and is considered irreversible. (biocat.no)