• volume = {122}, number = {Pt 24}, pages = {4505--4515}, abstract = {In Drosophila, the humoral response characterised by the synthesis of antimicrobial peptides (AMPs) in the fat body (the equivalent of the mammalian liver) and the cellular response mediated by haemocytes (blood cells) engaged in phagocytosis represent two major reactions that counter pathogens. (cnrs.fr)
  • The current paradigm is that peptides secreted in the hemolymph during the systemic immune response are either bona fide antimicrobial peptides or likely ones. (cnrs.fr)
  • Silencing of Velo led to constitutive expression of the IMD pathway dependent antimicrobial peptides (AMPs), and Escherichia coli stimulation further enhanced the AMP expression. (cnrs.fr)
  • S. aureus amidase (AM) protein, a cell wall hydrolase that cleaves the amide bond between N-acetylmuramic acid and L-alanine residue of the stem peptide, is several fold over-expressed under biofilm conditions. (bvsalud.org)
  • LytM is a glycyl-glycyl endopeptidase, hydrolyzing the pentaglycine interpeptide crossbridge, and SsaA belongs to the CHAP amidase family, members of which such as LysK and LytA have been shown to have D-alanyl-glycyl endopeptidase activity, cleaving between the crossbridge and the stem peptide. (hal.science)
  • Enzymes carrying NlpC/p60 domains, for instance RipA and RipB from Mycobacterium tuberculosis, are bacterial peptidoglycan hydrolases that cleave the peptide stems and contribute to cell wall remodelling during cell division. (scilifelab.se)
  • Peptide- N -Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N -linked glycoproteins (1). (neb.com)
  • The applicability of different enzymes such as glycosidases, glycosyltransferases, glycophosphorylases and glycosynthases have been de-scribed around the review article considering their advantages and disadvantages of these biocat-alysts in the stereoselective and regioselective synthesis of different types of glycosylated mole-cules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glyco-peptides and glycoproteins with a clear focus on food and pharmaceutical chemistry. (preprints.org)
  • In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. (wikipedia.org)
  • Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F". Biochemistry. (wikipedia.org)
  • We carried out a structure-based small molecule design using the crystal structure of AM protein catalytic domain to identify inhibitors that can block amidase activity and therefore inhibits S. aureus biofilm formation. (bvsalud.org)
  • A putative amidase gene encoding a 480 amino acid polypeptide was cloned and homologously expressed in A. niger. (scilifelab.se)
  • This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides. (wikipedia.org)
  • Two recently identified exo-lytic N -acetylmuramidases (Tf_NamZ1 and Tf_NamZ2) specifically cleave the peptide-free, exogenous (nutrition source) PGN in the periplasm and release the MurNAc and disaccharide substrates for the transporters Tf_MurT and Tf_AmpG, respectively, whereas the peptide-containing, endogenous (the self-cell wall) PGN stays unattached. (karger.com)
  • Salvage of polymeric PGN presumably requires the removal of peptides from PGN by an unknown amidase, concomitantly with the translocation of the polymer across the outer membrane. (karger.com)
  • Uncoupled expression of genes encoding lytic transglycosylases or amidases did not restore growth to a WalKR-depleted strain. (hal.science)
  • Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F". Biochemistry. (wikipedia.org)
  • Peptide-N 4 -( N -acetyl-β-glucosaminyl) asparagine amidase F (PNGase F) is an amidase/amidohydrolase that hydrolyzes the β-aspartylglycosylamine bond of asparagine-linked glycopeptides and glycoproteins. (go.jp)
  • This peptide also cleaves alpha 1,3 linkages, and has been named PNGase F-II. (wikipedia.org)
  • Type II pullulanases cleave both -1,4 and -1,6 glucosidic linkages in a variety of polysaccharides [21]. (bioinf.org)
  • This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides. (wikipedia.org)
  • (B) A schematic picture of an N-linked glycan and examples of bacterial enzymes that cleave carbohydrate residues, along with three examples of enzymes in the transit from bacterial pathogenesis to glycoengineering applications. (medscape.com)
  • The bacterial cell wall consists of a three-dimensional peptidoglycan layer, composed of peptides linked to the sugars N-acetylmuramic acid (MurNAc) and GlcNAc. (bvsalud.org)
  • Pullulanases cleave the -1,6 glucosidic bonds in pullulan, which is a linear polymer of maltotriose models joined by -1,6 glucosidic bonds. (bioinf.org)
  • A phosphorylated ADF peptide with the full TCGA cohorts to predict binding affinity prediction, named BiComp-DTA. (bethanchristopher.com)