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  • recombinant
  • Like E. coli GspS, the recombinant enzyme also possesses amidase activity (EC 3.5.1.78), hydrolysing glutathionylspermidine to GSH and spermidine with a k cat of 0.38s −1 and a K m of 500 μ M. GspS can also hydrolyse trypanothione at about 1.5% of the rate with glutathionylspermidine. (biochemj.org)
  • Here, recombinant production and export of B. megaterium ATCC14945 penicillin G amidase (PGA) which is used in the reverse synthesis of β-lactam antibiotics were systematically improved. (biomedcentral.com)
  • Lytic
  • The enzymatic activity of Rv3717 was confirmed by isolation and identification of its enzymatic products by LC/MS. These studies indicate that Rv3717, an N-acetylmuramoyl-L-alanine amidase from M. tuberculosis, represents a new family of lytic amidases that do not have a separate CBD and are regulated conformationally. (docphin.com)
  • Enzymes
  • Specific Structural Features of the N-Acetylmuramoyl-l-Alanine Amidase AmiD from Escherichia coli and Mechanistic Implications for Enzymes of This Family. (ac.be)
  • superfamily
  • However, the amidase differs from members of the nitrilase superfamily that are known to form spirals including nitrilase, cyanide dihydratase and cyanide hydratase by lacking two insertions (see figure1, supplementary material). (salamanderthemes.net)
  • mechanism
  • High resolution crystallographic studies by ourselves and others have suggested a mechanism for the closely related amidases that involves the use of not one, but two glutamates in addition to the cysteine and lysine. (salamanderthemes.net)
  • Our proposed amidase reaction mechanism is shown in figure 2 (see additional attachments). (salamanderthemes.net)
  • However
  • However, the final bioactivated phosphorothiolate can subsequently block the amidase activation step and thus inhibit further bioactivation, which probably contributes to the relatively low mammalian toxicity of acephate (Mahajna et al. (thefreedictionary.com)