Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). (thermofisher.com)
Molecule6
METHODS: Polyclonal and monoclonal antibodies were raised against a 13 amino acid peptide sequence from the cytoplasmic portion of the alpha chain of the human CD8 molecule. (bmj.com)
CD8 receptor binds to the class-I MHC molecule of the MHC 1 peptide(antigen) complex. (biotechfront.com)
CD8 is binds to the Alpha 3 domain of this MHC 1 molecule. (biotechfront.com)
Intact class I molecules consist of an alpha heavy chain bound to a beta-2 microglobulin molecule. (msdmanuals.com)
It contains the binding site for the T-cell accessory molecule CD8. (microbenotes.com)
Antigen2
Because every nucleated cell expresses class I MHC molecules, all infected cells can act as antigen-presenting cells for CD8 T cells (CD8 binds to the nonpolymorphic part of the class I heavy chain). (msdmanuals.com)
anti nf kb p65 antibody 783 anti stat6 antibody 784 anti cd 19 antibody 785 anti cd 38 antibody 786 anti pten ab 787 anti ck9 antibody 788 anti gh antibody 789 anti lh beta antibody 790 anti tsh alpha antibody 791 anti fsh alpha antibody 792 anti hpv antibody 793 anti ebv nuclear antigen ab 794 anti ebv latent memb. (tendersindelhi.com)
Binds3
CD8 alpha chains binds to class I MHC molecules alpha-3 domains. (lu.se)
CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. (thermofisher.com)
An interferon regulatory factor that binds upstream TRANSCRIPTIONAL REGULATORY ELEMENTS in the GENES for INTERFERON-ALPHA and INTERFERON-BETA . (lookformedical.com)
Chains7
CD8 is a dimeric glycoprotein consisting of either two alpha chains or an alpha and beta chain. (rndsystems.com)
CD8 Co-receptor is expressed as a dimer consisting of two polypeptide chains, Alpha chain and Beta chain. (biotechfront.com)
Both polypeptide chains are encoded by genes in the HLA-DP, -DQ, or -DR region of chromosome 6. (msdmanuals.com)
T-cell receptors composed of CD3-associated gamma and delta polypeptide chains and expressed primarily in CD4-/CD8- T-cells. (edu.au)
This class of the MHC class I is made up of two chains i.e a transmembrane glycoprotein with a molecular weight of 45,000, which is noncovalently associated with a non-MHC-encoded polypeptide with molecular weight of 12,000 that is known as β2-microglobulin. (microbenotes.com)
MHC Class I molecules in both human and mouse consist of two polypeptide chains that dramatically differ in size. (microbenotes.com)
Class II MHC molecules in both humans and mice consist of two polypeptide chains that have a similar, albeit not identical size. (microbenotes.com)
Transmembrane4
Various patterns of differential splicing of CD8 alpha transcripts involve excision of the transmembrane or cytoplasmic domains. (lu.se)
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (thermofisher.com)
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). (edu.au)
The transmembrane and the cytoplasmic domains ensure that the alpha chain spans the membrane and is properly expressed by the cell. (microbenotes.com)
Molecules5
This is based on the expression of two accessory molecules CD4 and CD8 on their plasma membrane. (biotechfront.com)
The T cells expressing CD8 molecules on the plasma membrane are designated as CD8 positive T cells . (biotechfront.com)
CD4 and CD8 molecules are known as Co-receptors . (biotechfront.com)
T cells that express CD8 molecules react with class I MHC molecules. (msdmanuals.com)
X-ray crystallography showed that the peptide-binding site in the MHC Class I molecules looks like a cleft that has a ''floor'' and two ''walls'' formed by spiral shaped portions of the alpha chain, called alpha 1 and alpha 2. (microbenotes.com)
Peptide3
Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence. (bmj.com)
AIMS: To evaluate whether cytotoxic/suppressor T cells can be detected in paraffin wax embedded human tissue samples using antibodies to a synthetic CD8 peptide sequence. (bmj.com)
Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (scancell.co.uk)
Genes2
Genes up-regulated in comparison of naive CD8 T cells versus naive CD4 [GeneID=920] T cells. (gsea-msigdb.org)
Although the expression of RAR alpha-target genes was suppressed in the kidneys of Tg26 mice and of patients with HIVAN, the expression of RAR alpha in the kidney was not different between patients with HIVAN and minimal change disease. (a-inhibitor.com)
Receptor5
alpha- and beta-interferon crossreact with common receptors, while gamma-interferon initiates its biological effects through its own specific receptor system. (lookformedical.com)
A ubiquitously expressed heterodimeric receptor that is specific for both INTERFERON-ALPHA and INTERFERON-BETA . (lookformedical.com)
The interferon alpha-beta receptor signals through the action of JANUS KINASES such as the TYK2 KINASE. (lookformedical.com)
Conversely, major histocompatibility complex class I polypeptide-related sequence A (MHC1A) (O.R.=0.6) and natural cytotoxicity triggering receptor 3 (NCR3) (O.R.=0.46) were associated with lower odds. (medrxiv.org)
differentiation markers by activating its receptor-alpha (RAR alpha). (a-inhibitor.com)
Cytotoxic2
RESULTS: These antibodies specifically detected the native form of the CD8 polypeptide when tested by immunoprecipitation with radiolabelled T cells, and gave the expected staining pattern for cytotoxic/suppressor T cells in cryostat sections. (bmj.com)
Furthermore, interferon leads to upregulation of MHC I and therefore to increased presentation of viral peptides to cytotoxic CD8 T cells, as well as to a change in the proteasome (exchange of some beta subunits by b1i, b2i, b5i - then known as the immunoproteasome) which leads to increased production of MHC I compatible peptides. (wikidoc.org)
Peptides1
Homocitrullinated peptides from aldolase and cytokeratin were identified, that stimulated CD8-mediated responses in vivo. (scancell.co.uk)
Residues2
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. (bvsalud.org)
One such modification is carbamylation (homocitrullination) that results in the formation of homocitrulline (Hcit) residues in a non-enzymatic reaction of cyanate with the lysine residues in the polypeptide chain. (scancell.co.uk)
Extracellular1
STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. (thermofisher.com)
Heterodimer1
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. (lu.se)
Interferons1
They include alpha- and beta-interferons ( INTERFERON-ALPHA and INTERFERON-BETA ). (lookformedical.com)
The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (CD3 COMPLEX). (edu.au)
Chain3
CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. (thermofisher.com)
The β-2-microglobulin chain is also vitally important for the proper expression of the alpha chain. (microbenotes.com)
CD8 responses were assessed in vitro for cytotoxicity and in vivo tumor therapy. (scancell.co.uk)
Importantly, in vivo the homocitrullinated aldolase specific response was associated with efficient CD8 dependent antitumor therapy of the aggressive murine B16 tumor model indicating that this epitope is naturally presented in the tumor. (scancell.co.uk)
Cells6
CD8 positive cells could also be detected in paraffin wax embedded tissues. (bmj.com)
Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-Human CD8 Magnetic Particles - DM according to the Magnetic Labeling Protocol. (bdbiosciences.com)
and CD8 + T cells play a major role as beta cell killers [ 6 , 7 ]. (springer.com)
COVID-19 plasma exosomes induced proinflammatory responses in CD4 + T cells, CD8 + T cells, and CD14 + monocytes but not significantly in regulatory T cells, Th17 T cells, or central memory T cells. (wphobby.com)
A pharmacological inhibitor of TLR3 considerably reduced cytokine and chemokine production by CD4 + and CD8 + T cells but not by CD14 + monocytes, highlighting divergent signaling pathways of immune cells in response to COVID-19 plasma exosomes. (wphobby.com)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
Responses1
Thus, Hcit-specific CD8 T-cell responses have potential in the development of future anticancer therapy. (scancell.co.uk)
Human4
Detects human CD8 alpha in direct ELISAs. (rndsystems.com)
Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution. (lu.se)
Leukocytes were labeled with BD IMag™ Anti-Human CD8 Magnetic Particles - DM (Cat. (bdbiosciences.com)
For flow cytometric analysis, fresh PBMC (left panel), the negative fraction (middle panel) and the positive fraction (right panel) were stained with PE Mouse Anti-Human CD8 (Cat. (bdbiosciences.com)
Positive1
No. 552311), and the negative (CD8-) and positive (CD8+) fractions were collected according to the accompanying protocol. (bdbiosciences.com)
Mice3
Here, we report that Am580, a water-soluble RAR alpha-specific agonist, attenuated proteinuria, glomerosclerosis, and podocyte proliferation, SN-38 cell line and restored podocyte differentiation markers in kidneys of Tg26 mice. (a-inhibitor.com)
Furthermore, RAR alpha-/- Tg26 mice developed more severe kidney and podocyte injury than did RAR alpha-/- Tg26 mice. (a-inhibitor.com)
injury in RAR alpha-/- Tg26 mice, confirming our hypothesis that Am580 acts through RAR alpha. (a-inhibitor.com)
Cell2
CD8 is thought to play a role in the process of T-cell mediated killing. (lu.se)
In addition, alpha cell dysfunction is recognised as a prominent feature of the disease [ 3 ]. (springer.com)
Potential1
Hence, RAR alpha agonists may be potential agents for the treatment of HIVAN. (a-inhibitor.com)
Mouse1
In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed. (rndsystems.com)
Gamma1
In early 2021, three variants B.1.1.7 (Alpha), B.1.351 (Beta) and P.1 (Gamma) were identified as variants of concern (VOC 1 ). (nature.com)