Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. (wikipedia.org)
The aim of the present study is to determine whether histone H3, αA-crystallin (CRYAA), β-galactosidase (GLB1), and p53 are involved in the pathogenesis of ARC. (techscience.com)
A total of 99 anterior lens capsules (ALCs) of patients with ARC of various nuclear grades, ultraviolet models of ALCs, and two human lens epithelial cell lines (FHL-124 and SRA01/04) were used, and the expression of histone H3, CRYAA, GLB1, and p53 were detected by immunoblotting and reverse transcription and real time-quantitative polymerase chain reaction. (techscience.com)
Destabilized proteins1
αA- and αB-crystallins are small heat shock proteins that bind thermodynamically destabilized proteins thereby inhibiting their aggregation. (nih.gov)
AlphaB-crystallin4
To elucidate the effect of deamidation on the structural and functional properties of human alphaB-crystallin. (nih.gov)
Site-directed mutagenesis was used to generate three deamidated mutants of alphaB-crystallin: N78D, N146D, and N78D/N146D. (nih.gov)
Recombinant native alphaB-crystallin (wild type [WT]) and the three mutated alphaB species were expressed, and each species was purified to homogeneity by ion-exchange chromatography followed by hydrophobic interaction chromatography. (nih.gov)
The results show that the deamidation of N146 but not of N78 have profound effects on the structural and functional properties of alphaB-crystallin. (nih.gov)
Lens15
Highly expressed in the mammalian lens, the α-crystallins have been postulated to play a critical role in the maintenance of lens optical properties by sequestering age-damaged proteins prone to aggregation as well as through a multitude of roles in lens epithelial cells. (nih.gov)
Here, we have examined the role of α-crystallins in the development of the vertebrate zebrafish lens. (nih.gov)
For this purpose, we have carried out morpholino-mediated knockdown of αA-, αBa- and αBb-crystallin and characterized the gross morphology of the lens. (nih.gov)
These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. (nih.gov)
A more consistent and severe lens phenotype was evident in maternal/zygotic αA-crystallin mutants compared to those observed by morpholino knockdown. (nih.gov)
The penetrance of the lens phenotype was reduced by transgenic expression of rat αA-crystallin and its severity was attenuated by maternal αA-crystallin expression. (nih.gov)
These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts. (nih.gov)
Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. (wikipedia.org)
alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. (wikipedia.org)
Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. (lookformedical.com)
Glycation of lens alpha-crystallin occurs in vivo and may contribute to cataractogenesis. (kansascity.edu)
Expression of the murine alpha B-crystallin gene is not restricted to the lens. (research.com)
His Cell biology research incorporates elements of Heat shock protein, Alpha-Crystallin A Chain, Apoptosis, Lens and Genetically modified mouse. (research.com)
To extend and confirm these findings, we generated an αA-crystallin knockout zebrafish line. (nih.gov)
Jan. 25, 2018-Studies of alpha-B crystallin in zebrafish could ultimately lead to improved treatment for cataracts and heart disease. (vumc.org)
HspX2
HspX or α-crystallin, a 16 kDa protein, is one of the dominant antigens expressed during the latent stages of M. tuberculosis infection and under various other conditions like low oxygen tension, nutrient starvation or hypoxia 7 , 8 . (nature.com)
This review describes the regulation and function of the major stress proteins within mycobacteria such as the GroEL, GroES and DnaK homologues along with hspX (alpha-crystallin) and others. (caister.com)
Glycation1
Methylglyoxal-induced glycation of alpha-crystallin caused aggregation as evidenced by increased 90 degrees light scattering. (kansascity.edu)
Recombinant10
Specificity: Natural and recombinant Bovine Alpha-lactalbumin GeneName: LALBA ProteinName: Alpha-lactalbumin Alternative: Lactose synthase B protein,ALACTA Assay. (researchd.com)
In this study, a genetically stable, marker-free recombinant MVA expressing α-crystallin of M. tuberculosis (rMVA. (nature.com)
ELISA5
Bovine NAGA/Alpha-N-acetylgalactosaminidase ELISA Kitis available at Gentaur for Next week Delivery. (researchd.com)
Bovine CSN1S2/Alpha-S2-casein ELISA Kitis available at Gentaur for Next week Delivery. (researchd.com)
Bovine ACTN4/Alpha-actinin-4 ELISA Kitis available at Gentaur for Next week Delivery. (researchd.com)
Bovine ADRA1A/Alpha-1A adrenergic receptor ELISA Kitis available at Gentaur for Next week Delivery. (researchd.com)
Description: A sandwich ELISA kit for detection of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (chemcarbindia.com)
Antigens2
Hence, boosting BCG with latency associated antigens like α-crystallin may prove to be an effective strategy for controlling TB. (nature.com)
These antigens have a heavy chain and an associated light chain. (medscape.com)
Molecular3
The scientist's investigation covers issues in Molecular biology, Cell biology, Crystallin, Heat shock protein and Alpha-Crystallin A Chain. (research.com)
His Crystallin research is multidisciplinary, relying on both Cytoplasmic inclusion, Glutathione and Molecular mass. (research.com)
Eric F. Wawrousek spends much of his time researching Cell biology, Immunology, Crystallin, Molecular biology and Inflammation. (research.com)
Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses. (lookformedical.com)
Gamma-crystallins are similar in structure to BETA-CRYSTALLINS in that they both form into a Greek key-like structure. (lookformedical.com)
His study explores the link between Crystallin and topics such as Knockout mouse that cross with problems in Anatomy. (research.com)
Vitro2
Carnosine Disaggregates Glycated Alpha-Crystallin: An In Vitro Study" by Norbert W. Seidler, George Yeargans et al. (kansascity.edu)
We investigated this hypothesis using glycated alpha-crystallin as our in vitro model. (kansascity.edu)
Peptide2
Additionally, carnosine decreased tryptophan fluorescence polarization of glycated alpha-crystallin, suggesting that carnosine increased peptide chain mobility, which may contribute to the controlled unfolding of glycated protein. (kansascity.edu)
Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. (cipsm.de)
Superfamily1
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. (caslab.com)
Polymerase Chain Re1
Sequence-specific primers with 3′ end mismatches were used to identify the presence of specific allelic variants by polymerase chain reaction (PCR) amplification. (molvis.org)
His Cell biology research is multidisciplinary, incorporating elements of CD28, Genetically modified mouse, Retinal and Cathepsin D. His Crystallin study combines topics in areas such as Mutant and Notch signaling pathway. (research.com)
His work carried out in the field of Retinal brings together such families of science as Crystallin, Notch signaling pathway and Optic nerve. (research.com)
Kinase1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) in Tissue homogenates, cell lysates and other biological fluids. (chemcarbindia.com)
Heat2
Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. (wikipedia.org)
α-crystallin is a member of the small heat shock protein family, which helps in maintaining and thickening of the cell wall and in providing stability to proteins that allow the bacteria to survive under harsher conditions 7 . (nature.com)
Antibodies1
To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Alpha Diagnostics Antibodies. (mymetroofficeproducts.net)
Regulation1
The present study was conducted to determine whether corneal cells differentially express genes for T4 regulation, keratan sulfate proteoglycan (KSPG) synthesis, crystallins, and endothelial cell ion transporters during transparency development and whether these expressions are altered when E9 embryos are treated with T4. (unboundmedicine.com)
Methods1
All six exons of crystallin, beta A4 gene ( CRYBA4 ) were amplified by PCR methods and direct sequencing. (molvis.org)
Expression3
Immunohistochemistry was performed to validate the expression of two upregulated (EpCAM, FADD) and two downregulated (NDRG1, αB-crystallin) proteins in 190 paraffin-embedded tissue samples. (biomedcentral.com)
Using quantitative RT-PCR we studied the expression of TRPC3, interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) in monocytes from 15 patients with essential hypertension and 16 age- and sex-matched normotensive control subjects. (shengsci.com)
Molecule1
Beta 2 microglobulin is an 11.8-kD protein (see first image below), which forms one of the chains of the major histocompatibility complex (MHC) class I molecule normally present on the surface of every nucleated cell in the human body. (medscape.com)
Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. (lookformedical.com)
Glycosyl hydrolases family 2, Beta galactosidase small chain, Domain of unknown function(DUF4981) [Interproscan]. (ntu.edu.sg)
This light-protein chain, which can be shed into serum, is called beta 2 microglobulin. (medscape.com)
Serum1
The Crp 16 A Antibody Alpha Diagnostics reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (mymetroofficeproducts.net)
Human1
Human IgG antibody Laboratories manufactures the crp 16 a antibody alpha diagnostics reagents distributed by Genprice. (mymetroofficeproducts.net)
Study2
In one of the study from our laboratory, we have shown that a DNA vaccine expressing α-crystallin provides considerable protection to guinea pigs against M. tuberculosis infection 12 . (nature.com)
In addition, a previous study from our laboratory based on BCG priming followed by boosting with the DNA vaccine expressing α-crystallin imparted markedly better protection against M. tuberculosis in comparison to BCG in both guinea pigs and mice 13 . (nature.com)