Alpha binding protein. Medical search. Frequent questions

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. (thermofisher.com)
also called orosomucoid) is synthesized in the liver and structurally belongs to the lipocalin superfamily of secretory proteins, such as retinol-binding protein and α1-microglobulin. (cdc.gov)

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)

Scholars@Duke publication: A 30-kDa alternative translation product of the CCAAT/enhancer binding protein alpha message: transcriptional activator lacking antimitotic activity. (duke.edu)
Full-length (42 kDa) CCAAT/enhancer binding protein alpha (C/EBP alpha) (p42) has been implicated in the transcriptional activation of adipocyte genes including the 422(aP2) and C/EBP alpha genes during differentiation of 3T3-L1 preadipocytes. (duke.edu)
2014. Deficiency of CCAAT/enhancer binding protein-epsilon reduces atherosclerotic lesions in LDLR-/- mice. . (oregonstate.edu)
Functional genomics data indicate that 2 of the suggestive SNPs (rs2446823 and rs2446824) are located within putative binding sites for the CCAAT/Enhancer Binding Protein (CEBP) and Hepatocyte Nuclear Factor 4, Alpha transcription factors (TFs), respectively. (cdc.gov)

We report here that the nucleocapsid (N) protein of HTNV was able to inhibit TNF-α-induced activation of NF-κB, as measured by a reporter assay, and the activation of endogenous p65, an NF-κB subunit. (mssm.edu)
Tetracycline inhibits bacterial protein synthesis by binding with 30S and, possibly, 50S ribosomal subunit(s). (medscape.com)

Filamin A primarily attaches (binds) to another protein called actin and helps it form the branching network of filaments that make up the cytoskeleton. (medlineplus.gov)
Like other transcription factors, the RUNX1 protein attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (medlineplus.gov)

Iron-binding proteins are carrier proteins and metalloproteins that are important in iron metabolism and the immune response. (wikipedia.org)

Description: A sandwich ELISA kit for detection of C4 Binding Protein Alpha from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (tissue-cell-culture.com)

GTP-Binding Protein alpha Subunits" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
The GTPase-containing subunits of heterotrimeric GTP-binding proteins. (jefferson.edu)
The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems. (jefferson.edu)
This graph shows the total number of publications written about "GTP-Binding Protein alpha Subunits" by people in this website by year, and whether "GTP-Binding Protein alpha Subunits" was a major or minor topic of these publications. (jefferson.edu)
Below are the most recent publications written about "GTP-Binding Protein alpha Subunits" by people in Profiles. (jefferson.edu)
It is a tetramer consisting of two alpha and two beta subunits. (wikipedia.org)
Oxygen binding is fully cooperative for each of the subunits because as the first oxygen binds to one of the four heme groups, the protein undergoes a drastic conformational change that sharply increases the oxygen affinity of the other three subunits. (wikipedia.org)
In vitro binding studies suggested that binding of j4R to alpha NAC did not interfere with the binding of alpha- and beta NAC subunits (the BTF3 transcription factor). (ox.ac.uk)

Nickel ABC transporter, substrate-binding protein NikA / Solute-binding protein family 5, conserved site / Bacterial extracellular solute-binding proteins, family 5 signature. (pdbj.org)

However, analogous proteins may have structural homology although this is not a prerequisite. (hindawi.com)
Here, we designed a set of homology-directed repair donor templates that enable efficient tagging of endogenous proteins with affinity tags by transient transfection and selection of genome-edited cells in various human cell lines. (bvsalud.org)

Estrogen receptor-alpha (ERalpha) promotes proliferation of breast cancer cells, whereas tumor suppressor protein p53 impedes proliferation of cells with genomic damage. (nih.gov)
also folate receptor alpha and folate binding protein 1/FOLBP1) is a 40-50 kDa member of the folate receptor family. (rndsystems.com)
Kada transferinski protein napunjen gvožđem naiđe na transferinski receptor na površini ćelije , npr. (wikipedia.org)
Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). (rndsystems.com)

In vitro translation of wild-type C/EBP alpha mRNA or transient transfection with a wild-type C/EBP alpha vector gave rise to similar levels of p42 and p30. (duke.edu)
Although transfection of 3T3-L1 preadipocytes with a strong p30C/EBP alpha expression vector is insufficient to induce differentiation, this vector advances the differentiation program. (duke.edu)

Marques JT , Trindade GS , da Fonseca FG , dos Santos JR , Bonjardim CA , Ferreira PCP , Characterization of ATI, TK and IFN-alpha/betaR genes in the genome of the BeAn 58058 virus, a naturally attenuated wild orthopoxvirus. (cdc.gov)
The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
RafK carries a C-terminal regulatory domain present in a subset of ATP-binding proteins that has been involved in both direct regulation of transporter activity (inducer exclusion) and transcription of transporter genes. (lu.se)
Pneumococci lacking RafK showed a 50- to 80-fold reduction in expression of the raf operon genes aga (alpha-galactosidase) and rafEFG (raffinose substrate binding and permease genes), and both glucose and sucrose inhibited raffinose uptake through inducer exclusion. (lu.se)

This protein interacts with another protein called core binding factor beta or CBFβ (produced from the CBFB gene), which helps RUNX1 bind to DNA and prevents it from being broken down. (medlineplus.gov)
It interacts also with anticoagulant protein S and with serum amyloid P component. (lu.se)

Unlike p42C/EBP alpha, which inhibits cell proliferation, p30C/EBP alpha is not antimitotic. (duke.edu)
Dive into the research topics of 'Hantaan virus nucleocapsid protein binds to importin α proteins and inhibits tumor necrosis factor alpha-induced activation of nuclear factor kappa B'. Together they form a unique fingerprint. (mssm.edu)
Metformin Enhances the Effect of Regorafenib and Inhibits Recurrence and Metastasis of Hepatic Carcinoma After Liver Resection via Regulating Expression of Hypoxia Inducible Factors 2α (HIF-2α) and 30 kDa HIV Tat-Interacting Protein (TIP30). (cancerindex.org)
Azathioprine antagonizes purine metabolism and inhibits synthesis of DNA, RNA, and proteins. (medscape.com)

Adducin, an alpha/beta heterodimeric protein found in the renal tubule is thought to regulate ion transport through changes in the actin cytoskeleton. (nih.gov)

In both human cells in a dish (in vitro) and living mouse brains (in vivo) alpha-synuclein was present in the exact same location as the DNA repair proteins, suggesting alpha-synuclein binds directly to DSBs, and helps repair those breaks. (parkinson.org)
DLDH also bound directly to RafK both in vitro and in vivo, indicating the possibility that DLDH regulates raffinose transport by a direct interaction with the regulatory domain of the transporter. (lu.se)

Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)

Filamin A is also involved in the organization of the extracellular matrix, which is the lattice of proteins and other molecules outside the cell. (medlineplus.gov)
Filamin A binds to proteins called integrins, which span the cell membrane and anchor cells to the extracellular matrix. (medlineplus.gov)
Through this binding, cells are correctly positioned and signals can be exchanged between the cell and the extracellular matrix. (medlineplus.gov)

Scope includes mutations and abnormal protein expression. (cancerindex.org)
NEMO consists of 10 exons, and most mutations cause deletions of exons 4-10, resulting in a truncated protein. (medscape.com)
Hypomorphic mutations may impair but not abolish NEMO protein function. (medscape.com)

Because this genetic change affects CBF, the condition is classified as core binding factor AML (CBF-AML). (medlineplus.gov)

However, molecular weight is also important due to potential steric incompatibilities within protein cores. (hindawi.com)

In addition, structural data confirms the paradoxical behavior of glucose, where it replaces the high-affinity ligand(s) (disaccharide α-glycosides) from the active site of the protein. (rcsb.org)
Structural basis for Hif-1 alpha /CBP recognition in the cellular hypoxic response. (expasy.org)
SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
Combined with technological advancements in single-particle cryogenic electron microscopy, this strategy allows efficient structural studies of endogenous proteins captured in their native cellular environment and during different cellular processes. (bvsalud.org)

Altered G Protein Coupling in Olfactory Neuroepithelial Cells From Patients With Schizophrenia. (jefferson.edu)
The FLNA gene provides instructions for producing the protein filamin A, which helps build cells' extensive internal network of protein filaments called the cytoskeleton. (medlineplus.gov)
The cytoskeleton is also important for certain processes inside the cells, such as the movement of proteins within the cell and the breakdown of unneeded proteins. (medlineplus.gov)
Filamin A can also bind to many other proteins in the cell to carry out various functions, including the attachment of cells to one another (cell adhesion), cell movement (migration), determination of cell shape, the relay of signals within cells, and cell survival. (medlineplus.gov)
The African swine fever virus (ASFV) j4R protein is expressed late during the virus replication cycle and is present in both the nucleus and the cytoplasm of infected cells. (ox.ac.uk)
By using the yeast two-hybrid system, direct binding, and coprecipitation from cells, we showed that the j4R protein binds to the alpha chain of nascent polypeptide-associated complex (alpha NAC). (ox.ac.uk)
Confocal microscopy indicated that a proportion of j4R and alpha NAC interact in areas close to the plasma membrane, as well as through the cytoplasm in cells. (ox.ac.uk)
Surprisingly, there was no defect in the degradation of the inhibitor of NF-κB (IκB) protein, nor was there any alteration in the level of p65 expression in HTNV N-expressing cells. (mssm.edu)
However, immunofluorescence antibody staining demonstrated that cells expressing HTNV N protein and a green fluorescent protein-p65 fusion had limited p65 nuclear translocation. (mssm.edu)
These clumping proteins are called Lewy Bodies that can be found in an area of the brain stem where dopamine cells die. (parkinson.org)
However, what if the unhealthy alpha-synuclein (which becomes Lewy Bodies) causes the loss of DSB repair, leading to healthy cells dying? (parkinson.org)
Researchers then measured the amount of DSBs in healthy human cells and human cells where alpha-synuclein was completely removed (known as knock-out cells). (parkinson.org)
The researchers found that the cells without alpha-synuclein (knock-out cells) had higher levels of DSBs compared to healthy cells, suggesting that alpha-synuclein plays a role in repairing DSBs. (parkinson.org)
They also found that the healthy human cells repaired DSBs more rapidly compared to the knock-out cells, again supporting the role of alpha-synuclein in aiding DNA repair. (parkinson.org)
In both healthy human cells and living mouse brains, the laser-induced DSBs, triggered alpha-synuclein to move to the site of DNA damage. (parkinson.org)
In diseased human cells and mice carrying the abnormal form of alpha-synuclein, the laser-induced DSBs, impaired alpha-synuclein from moving to the site of DNA damage. (parkinson.org)
Removing alpha-synuclein in human cells lead to increased DSB levels after receiving chemotherapy drug (bleomycin) treatment, and a reduction in the ability to repair these DSBs, compared to healthy human cells. (parkinson.org)
Giving healthy human alpha-synuclein to the alpha-synuclein knock-out mice, restored the mice cells' DNA damage response to normal levels. (parkinson.org)
[ 6 ] NF-KB protects cells from apoptosis in response to tumor necrosis factor-alpha (TNF-alpha). (medscape.com)
We demonstrated this strategy by tagging six different human proteins in both HEK293T and Jurkat cells. (bvsalud.org)

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. (therabio.org)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human C4 Binding Protein Alpha (C4BPa) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. (tissue-cell-culture.com)
Description: Quantitative sandwich ELISA for measuring Human C4 Binding Protein Alpha (C4BPA) Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (tissue-cell-culture.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat C4 Binding Protein Alpha (C4BPa) in serum, plasma and other biological fluids. (tissue-cell-culture.com)
Detection of the Tau protein in human serum by a sensitive four-electrode electrochemical biosensor. (mdx.ac.uk)

Clusters are often associated with cysteine residues in the protein chain. (wikipedia.org)
It is composed of approximately 700 residues and exists mainly as a tetramer, with the monomer:tetramer ratio being 1:4 at 10 μM protein concentrations. (wikipedia.org)
The long alpha-helical tail from residues 54 to 355 was shown to be involved in self-interactions, whereas the region from residue 54 to 177 was involved in heterodimerization with KfrC, another RA3-encoded alphahelical protein. (waw.pl)
VH is derived from a single protein domain of 35 residues [ 9 ]. (hindawi.com)

Heme proteins are proteins that contain a heme prosthetic group. (wikipedia.org)
Each of the four monomeric units contain a heme prosthetic group in which a ferric cation is bound between four nitrogen atoms of a porphyrin ring. (wikipedia.org)
These proteins act as electron shuttles by switching the oxidation state of the heme iron atom between ferrous (Fe2+) and ferric (Fe3+). (wikipedia.org)

What pathways are this gene/protein implicaed in? (cancerindex.org)
AMPK pathways inhibit biosynthetic pathways with energy consumption, such as protein, fatty acid and glycogen synthesis. (springer.com)

Researchers suspect that the variants involved in frontometaphyseal dysplasia may change the way the filamin A protein helps regulate processes involved in skeletal development, but it is not known how changes in the protein relate to the specific signs and symptoms of the condition. (medlineplus.gov)

For both, disease pathogenesis is thought to be immunologically mediated and there have been numerous reports of patients with elevated levels of proinflammatory and inflammatory cytokines, including tumor necrosis factor alpha (TNF-α), in their sera. (mssm.edu)

Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. (nih.gov)
During differentiation of 3T3-L1 preadipocytes and during hepatocyte development, the cellular p42C/EBP alpha/p30C/EBP alpha ratio changes, raising the possibility of a regulatory role. (duke.edu)

An example of this is in hemoglobin, where the porphyrin works together with a histidine side chain and a bound O2 molecule, forming an octahedral complex. (wikipedia.org)
Furthermore, we were able to detect an interaction between HTNV N protein and importin α, a nuclear import molecule responsible for shuttling NF-κB to the nucleus. (mssm.edu)

The tertiary structure is composed of two lobes, termed N and C lobes, each containing one iron-binding pocket. (wikipedia.org)

Hemoglobin has a binding affinity for carbon monoxide that is 250 times greater than for oxygen. (wikipedia.org)

Protein design methods use trial and error or more sophisticated methods like directed evolution or inverse folding to generate novel scaffolds or to find novel protein sequences folding into a defined scaffold, respectively. (hindawi.com)
Given the intimate relationship between a protein's structure and function, a way to design proteins with targeted properties is to start from a desired structure and find sequences able to fold into it, imposing additional constraints in the process [ 1 ]. (hindawi.com)
[18] The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. (wikipedia.org)

The fibroblasts then produce hyaluronan and the cytokines interleukin (IL)-1 beta, IL-6, IL-8, IL-16, tumor necrosis factor (TNF) alpha, and CD4 ligand. (medscape.com)

Computational design of novel proteins with well-defined functions is an ongoing topic in computational biology. (hindawi.com)

The RUNX1 gene provides instructions for making a protein called runt-related transcription factor 1 (RUNX1). (medlineplus.gov)
Activation of the transcription factor nuclear factor KB (NF-KB) requires the NEMO protein. (medscape.com)

Collectively, our data suggest that HTNV N protein can sequester NF-κB in the cytoplasm, thus inhibiting NF-κB activity. (mssm.edu)

The TNF-alpha binds and results in altered regulation of pro-inflammatory and protective proteins. (medscape.com)
Overlap of 2 suggestive SNPs with likely TF binding sites suggests possible roles in disruption of gene regulation. (cdc.gov)

Localization of the DNA-binding helix-turn-helix motif (HTH) was mapped to the N29-R52 region by protein structure modeling and confirmed by alanine scanning. (waw.pl)

Hydrophobicity was one of the most important physicochemical properties, due to the fact that it is involved in protein interactions, for example, by forming hydrophobic cores. (hindawi.com)

p30C/EBP alpha binds to the C/EBP sites within and activates reporter gene expression driven by the 422(aP2) and C/EBP alpha gene promoters. (duke.edu)

Lastly, they tried adding healthy human alpha-synuclein back into the mice without alpha-synuclein to see if it might restore the normal DNA damage response. (parkinson.org)
Removing alpha-synuclein in mice (the knock-out mice) also resulted in increased DSBs, following bleomycin treatment. (parkinson.org)

Import of these sugars occurs through the previously characterized raffinose ATP-binding cassette (ABC) transport system, encoded by the raf operon, that lacks the necessary ATP-binding protein. (lu.se)

The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. (nih.gov)
One of the methods that have been proposed is a multiobjective optimization, in which protein stability and catalytic activity are simultaneously optimized [ 4 , 5 ]. (hindawi.com)
Several methods have been proposed to design novel stable proteins, such as multi-objective optimization, in which protein stability and catalytic activity are simultaneously optimized. (hindawi.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)

In this work, we generated and optimized a new synthetic fusion protein using an evolutionary approach. (hindawi.com)

Protein se sastoji od alfa-heliksa i beta-listova koji formiraju dva domena . (wikipedia.org)
Disulfide-linked complex of C4bp alpha and beta chains of 3 possible sorts: a 570 kda complex of 7 alpha chains and 1 beta chain, a 530 kda homoheptamer of alpha chains or a 500 kda complex of 6 alpha chains and 1 beta chain. (lu.se)

This study reports crystal structures of an α-glycoside-binding protein (αGlyBP, ORF ID: TTHA0356 from Thermus thermophilus HB8) in complex with disaccharide α-glycosides namely trehalose (α-1,1), sucrose (α-1,2), maltose (α-1,4), palatinose (α-1,6) and glucose within a resolution range of 1.6-2.0 Å. (rcsb.org)
Oxygen binds to the empty sixth position to form an octahedral complex in the holo form. (wikipedia.org)
The African swine fever virus protein j4R binds to the alpha chain of nascent polypeptide-associated complex. (ox.ac.uk)
Together, these proteins form one version of a complex known as core binding factor (CBF). (medlineplus.gov)
The NEMO protein is required for the activation of the kinase complex. (medscape.com)

Anatomy18

Organisms13

Diseases1

Chemicals and Drugs143

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes55

Disciplines and Occupations1

Information Science3

SuperFamily2

Recombinant Proteins1

CCAAT4

Subunit2

Attaches2

Carrier Proteins1

ELISA Kit1

Subunits8

Substrate-binding1

Homology2

Receptor4

Transfection2

Genes4

Interacts2

Inhibits4

Actin1

Vitro2

Metabolism1

Extracellular3

Mutations3

Genetic1

Molecular Weight1

Structural4

Cells20

Serum6

Residues4

Heme3

Pathways2

Regulate1

Tumor necro1

Cellular2

Molecule2

Tertiary1

Carbon monoxide1

Sequences3

Cytokines1

Computational1

Transcription2

Cytoplasm1

Regulation2

Localization1

Interactions1

Activates1

Mice2

Cassette1

Stability4

Fusion protein1

Beta2

Complex5