Results In this study, we uncover an adaptive resistance mechanism to PARP inhibition mediated by tumor-associated macrophages (TAMs) in the TME. (bmj.com)
Mechanistically, PARP inhibition elevates the STAT3 signaling pathway in tumor cells, which in turn promotes protumor polarization of TAMs. (bmj.com)
STAT3 ablation in tumor cells mitigates polarization of protumor macrophages and increases tumor-infiltrating T cells on PARP inhibition. (bmj.com)
HCC-derived exosomes participate in changing tumor progression by regulating the tumor microenvironment and tumor immune status [ 14 , 15 ]. (hindawi.com)
The association between EBV gene expression and inhibition of MHC class I HLA expression implies that antigen display is either directly inhibited by EBV, facilitating immune evasion by tumor cells, and/or that tumor cells with inhibited presentation are selected for their ability to sustain higher levels of EBV to take maximum advantage of EBV oncogene-mediated tumor-promoting actions. (gsea-msigdb.org)
Prostate cancer cells1
Ectopic expression of Wnt5a in prostate cancer cells attenuates the antiproliferative effect of AR inhibition, while its suppression in drug-resistant cells restores partial sensitivity, a correlation also evident in an established mouse model. (uth.edu)
Biological2
Description: A competitive Inhibition ELISA kit for detection of Ghrelin from Rabbit in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (myelisakit.com)
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Pig Ghrelin (GHRL) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (myelisakit.com)
Expression2
ALOX5 arachidonate 5-lipoxygenase: macrophages infected with Candida albicans, Aspergillus flavus or Aspergillus fumigatus or treated with Curdlan, a selective agonist of pattern recognition receptor for fungi Dectin-1, displays increased expression of ALOX5, ALOX15 and PTGS2 Karnam et al, 2015 . (polygenicpathways.co.uk)
Among the human genes whose inhibition was most strongly correlated with increased EBV gene expression were multiple MHC class I HLA genes involved in regulating immune response via antigen presentation. (gsea-msigdb.org)
Role1
Arachidonic acid 15-lipoxygenases (ALOX15) play a role in mammalian erythropoiesis but they have also been&nb. (cn1699.cn)
Pharmacological inhibition of 122
According to Dr. Klein, "These results indicate that pharmacological inhibition of 12/15-LO in vivo can improve bone mass and strength during skeletal development as well as offset the bone loss that accompanies estrogen deficiency. (nih.gov)
The goal of this study was to evaluate the effect of pharmacological inhibition of 12/15LO in the triple transgenic mice (3xTg), which develop amyloid plaques and tau tangles together with significant memory and learning deficits. (nih.gov)
Effect of pharmacological1
In a further phase of the study, the researchers investigated the effect of pharmacological inhibitors of the 12/15-LO enzyme. (nih.gov)
Vivo2
However, while ZOL-induced growth inhibition was similar between the tumor groups in vivo, ZOL enhanced B and T lymphocyte infiltration into the orthotopic tumors with down-regulated CD73. (bvsalud.org)
In addition, reduction of 15-LO-1 in vivo significantly alleviated OA. (nature.com)
Mediators1
15-Lipoxygenase-1 (15-LO-1) belongs to a lipoxygenase family of enzymes that catalyze the stereospecific addition of molecular oxygen across double bonds in polyunsaturated fatty acids to form a series of biologically active lipid mediators. (nature.com)
Lipoxygenase4
NECD also appears due to the non-allergic action of NSAIDs in inhibiting the production of COX anti-inflammatory metabolites while promoting the production 5-lipoxygenase and 15-lipoxygenase pro-inflammatory metabolites and the overproduction of certain pro-allergic chemokines, e.g. eotaxin-1, eotaxin-2, RANTES, and interleukin-5. (wikipedia.org)
Subsequently, their studies of the genes located within this region showed that the Alox15 gene, which produces an enzyme called 12/15-lipoxygenase (12/15-LO), was much more active in the low-bone mass strain than in the high-bone mass strain. (nih.gov)
15-Lipoxygenase-1 (15-LO-1) is involved in many pathological processes. (nature.com)
Metabolites1
9 15( S )-hydroperoxyeicosatetraenoic acid (15( S )-HETE) and 13( S ) hydroperoxyoctadecadienoic acid (13( S )-HODE) are primary metabolites of arachidonic acid and linoleic acid catalyzed by 15-LO-1, respectively. (nature.com)
Enzyme4
These drugs had already been developed to inhibit 12/15-LO in other conditions, such as atherosclerosis (clogged arteries), cancer, and asthma, in which this enzyme is also thought to play a role. (nih.gov)
17. Expression of DHA-Metabolizing Enzyme Alox15 is Regulated by Selective Histone Acetylation in Neuroblastoma Cells. (nih.gov)
Silencing 15-LO-1 was able to markedly alleviate mechanical loading-induced cartilage ECM secretion, cartilage-degrading enzyme secretion and ROS production. (nature.com)
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat Amyloid Beta Peptide 1-42 (Ab1-42) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. (myelisakit.com)
Acid2
Arachidonic acid ( AA ), i.e. 5 Z , 8 Z ,11 Z ,14 Z -eicosatetraenoic acid is an ω-6 fatty acid with four double bonds in the cis configuration (see Cis-trans isomerism ), each located between carbons 5-6, 8-9, 11-12, and 14-15. (thcscience.wiki)
The inhibition constant of L-ascorbic acid was also obtained, which seemed to imply that enhancing reaction temperature could depress the substrate inhibition. (nadph-oxidasesignaling.com)
Expression5
14. Inhibition of histone acetyltransferase by naringenin and hesperetin suppresses Txnip expression and protects pancreatic β cells in diabetic mice. (nih.gov)
The levels of 15-LO-1 expression were measured by western blotting and quantitative real-time PCR in articular cartilage from the OA rat models and OA patients. (nature.com)
To further investigate the effects of 15-LO-1 on chondrocyte functions, such as extracellular matrix (ECM) secretion, the release of matrix-degrading enzymes, the production of reactive oxygen species (ROS), cell proliferation and apoptosis, we decreased or increased 15-LO-1 expression in chondrocytes by means of transfecting with siRNA targeting 15-LO-1 and plasmid encoding 15-LO-1, respectively. (nature.com)
The results showed that 15-LO-1 expression was obviously increased in articular cartilage from OA rats and OA patients. (nature.com)
It was also found that many factor-related OA, such as mechanical loading, ROS, SNP and inflammatory factor, significantly promoted 15-LO-1 expression and activity in chondrocytes. (nature.com)
Survival1
Since tumor tissue has a higher demand for copper and is more susceptible to copper homeostasis, copper may modulate cancer cell survival through reactive oxygen species (ROS) excessive accumulation, proteasome inhibition and anti-angiogenesis. (mdpi.com)
Results3
The results of this study merit further animal and human investigation of the role of 12/15-LO in the development of osteoporosis. (nih.gov)
Coupled with published data, the results suggest that inhibition of activation of PKR or its effect on viral replication is staged early in infection by VHS, postsynthesis of VP16 and VP22 by the gamma(1)134.5 protein, and very late in infection by the U(s)11 protein. (micrornamimic.com)
Taken together, these results indicate that 15-LO-1 has an important role in the disease progression of OA. (nature.com)
Effects1
Follow-up experiments confirmed the effects of 12/15-LO on bone mass in mice. (nih.gov)
Target1
Thus 15-LO-1 may be a good target for developing drugs in the treatment of OA. (nature.com)
Cell3
ZOL-induced tumor growth inhibition was found to be augmented with B cell depletion in sh-NT tumors, but not in sh-CD73 tumors. (bvsalud.org)
Description: A competitive Inhibition ELISA kit for detection of Amyloid Beta Peptide 1-42 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (myelisakit.com)
Description: A competitive Inhibition ELISA kit for detection of Corticotropin Releasing Hormone from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (wildpalm.net)