RejectionTransplantationSprague-DawleyRenalTransplantOrthotopicWistarDysfunctionLungTherapeuticSurvivalAntigenLiverFibrosisHistologicalVascularBoneCyclosporineAirway1987TumorMurineOrganHypoxicExperimentalUnderwentImmunosuppressiveFemursPathwayRecombinantInhibitionReconstructionInduceModelFunctionalVivoEffectivelyResults
Rejection30
- As the simultaneous transplantation of two or more organs into a single recipient has become increasingly common, asynchronous allograft rejection has become an important clinical problem. (northwestern.edu)
- To investigate the cellular and molecular mechanisms underlying differential organ rejection, we developed a rat model in which heart and lung allografts were transplanted sequentially. (northwestern.edu)
- These animals were then given a second organ allograft from the same strain or a third party to assess whether they exhibited donor specific tolerance and whether the acceptance or rejection of the first allograft would influence the survival of the second transplant. (northwestern.edu)
- We conclude that major histocompatibility complex antigens as well as other antigens are involved in the differential rejection of heart and lung allografts. (northwestern.edu)
- In the present study, we investigated whether viral infections could induce airway damage in rat lung transplants in the absence or presence of chronic rejection. (rug.nl)
- This study shows that a respiratory viral infection aggravates the airway damage in rat lung allografts with chronic rejection. (rug.nl)
- This study was to investigate the expression of IL-22 mRNA in the corneal grafts and the role of IL-22 in the immune rejection after corneal transplantation in rats. (cjeo-journal.org)
- The median survival time of grafts in the allograft keratoplasty group was 10 days, and that in the anti-rejection group was 17 days, showing a significant survival extention in the anti-rejection group ( χ 2 =16.442, P =0.000). (cjeo-journal.org)
- The expression level of IL-22 RNA up-regulates in the grafts with immuno-rejection.Topical administration of tobramycin and dexamethasone eye drops inhibits the rejection after keratoplasty.AhR plays a regulative role to the expression of IL-22 in rats after keratoplasty. (cjeo-journal.org)
- Allograft Rejection in the Middle Ear of the Rat. (wikipedia.org)
- The focus of the Streblow laboratory is on defining the role of human cytomegalovirus (HCMV) in the development of vascular disease and chronic rejection of organ allografts. (ohsu.edu)
- Determine viral and host gene expression during the development of atherosclerosis and transplant vascular sclerosis, the vascular lesion associated with chronic allograft rejection. (ohsu.edu)
- Determining the mechanisms of viral acceleration of chronic allograft rejection from latently infected donor, which is the most common cause of HCMV-associated disease in transplant patients associated with expression during the different stages of viremia associated with CMV including. (ohsu.edu)
- Renal biopsy remains the gold-standard for detecting allograft rejection but is associated with significant morbidity and mortality. (hindawi.com)
- Several animal models of allograft rejection exhibit monocyte and macrophage infiltration in allograft tissue [ 13 - 17 ], and these cells have a central role in human chronic allograft damage [ 18 , 19 ]. (hindawi.com)
- CD103 is thought to play a role in allograft rejection. (bdbiosciences.com)
- Mycophenolate mofetil has also been shown to reverse ongoing acute rejection in the canine renal and rat cardiac allograft models. (nih.gov)
- Combined use of the JAK3 inhibitor CP-690,550 with mycophenolate mofetil to prevent kidney allograft rejection in nonhuman primates. (lclabs.com)
- My research focuses on the molecular mechanisms and biomarkers of rejection after allograft transplantation. (stanford.edu)
- I am currently conducting surgeries to gain insight into chronic rejection after liver transplants in rat liver transplant models, as well as high-dimensional mass cytometry (CyTOF) analysis using the samples of vascularized composite tissue transplant to discern cell populations and subsets involved in the rejection. (stanford.edu)
- Allograft inflammatory factor-1 (AIF-1 or Iba1) is a 17kDa cytoplasmic, cytokine-induced, calcium-binding protein first discovered in rat cardiac allografts with chronic rejection. (ihcworld.com)
- All of these factors are known to result in myocardial apoptosis(5) and the acceleration of allograft rejection or chronic allograft dysfunction. (researchsquare.com)
- BALB/c → C57BL/6 allografts were reconstituted with iPSC-derived MSCs (2 million/transplant/at d0), and allografts were examined for regulatory T cells (Tregs), oxygenation, microvascular blood flow, airway epithelium, and collagen deposition during rejection. (biomedcentral.com)
- Dr. Solomon's research interests include endothelial cell dysfunction and inflammation in pulmonary arterial hypertension, methods of detection of cardiac allograft cellular rejection, and organizational structures and outcomes in the delivery of critical care cardiology. (nih.gov)
- Therefore, the differential ability of dendritic cells and macrophages (splenic adherent cells [SACs]) to stimulate pancreatic islet allograft rejection in reversed alloxan-induced diabetic rats was examined. (diabetesjournals.org)
- Rats bearing established allografts were challenged with various dosages of donor-strain dendritic cells or SACs, and graft rejection was assessed by analysis of plasma glucose levels and/or histological criteria. (diabetesjournals.org)
- Previous studies indicated that as few as 10 3 dendritic cells initiate allograft rejection in nondiabetic recipients. (diabetesjournals.org)
- In 1944, Medawar showed that skin allograft rejection is a host versus graft response. (medscape.com)
- The degree to which allografts undergo rejection depends partly on the degree of similarity or histocompatibility between the donor and the recipient. (medscape.com)
- Histocompatibility antigens are encoded on more than 40 loci, but the loci responsible for the most vigorous allograft rejection reactions are on the major histocompatibility complex (MHC). (medscape.com)
Transplantation7
- Heterotopic heart allografts transplanted into DA recipients from PVG donors survived indefinitely if the recipients were given a short course of rabbit antirat thymocyte globulin or cyclosporine at the time of transplantation. (northwestern.edu)
- Six months after transplantation, 4 noninfected rats of each group were killed for histological investigation (another 4 noninfected allografted rats were killed 56 days later). (rug.nl)
- We found marked induction of HO mRNA and protein in renal allografts at day 5 after transplantation. (elsevierpure.com)
- Determining the mechanisms of CMV-accelerated vascular disease in both mouse models of atherosclerosis and rat organ transplantation models using a DNA microarray approach. (ohsu.edu)
- Two cohorts of mice underwent renal transplantation with either a syngeneic isograft or allograft kidney. (hindawi.com)
- Tofacitinib effectively blocked allograft vasculopathy in the rat model of aorta transplantation by inhibiting JAK3. (lclabs.com)
- Transplantation of vascular allograft. (fishpond.com)
Sprague-Dawley4
- Lung transplants were performed using Lewis donors and Sprague-Dawley recipients (allografts) or the same strain (isografts). (nih.gov)
- In the present investigation, we successfully implanted rat C6 cells via intracranial stereotaxic cannulation in adult Sprague-Dawley rats for development and histoimmunopathological characterization of an advanced orthotopic glioblastoma allograft model, which could be useful for investigating the course of glioblastoma development as well as for testing efficacy of new therapeutic agents. (elsevierpure.com)
- Our investigation indicated the successful development of intracranial cannulated orthotopic glioblastoma allograft in adult Sprague-Dawley rats, making it as a useful animal model of glioblastoma for pre-clinical evaluation of various therapeutic strategies for the management of glioblastoma. (elsevierpure.com)
- A cardiac fibrosis model was established in Sprague-Dawley rats through a subcutaneous injection of isoproterenol (ISO, 5 mg/kg/d). (dovepress.com)
Renal6
- Prominent expression of HO protein, as detected by immunofluorescence, was observed in the mononuclear cells infiltrating the renal allograft. (elsevierpure.com)
- Our studies provide the first demonstration of the induction of HO in the rejecting renal allograft as well as the first demonstration in vivo for the induction of HO in macrophages at the site of an inflammatory response. (elsevierpure.com)
- We investigated whether ultrasmall paramagnetic particles of iron oxide- (USPIO-) enhanced magnetic resonance imaging (MRI) can detect experimental chronic allograft damage in a murine renal allograft model. (hindawi.com)
- The role of monocytes and macrophages in chronic renal allograft damage has been well established [ 9 ]. (hindawi.com)
- Monocytes and macrophages are known to play a role in chronic renal allograft damage [ 10 ] and are key promoters of fibrosis in other organs, such as the liver [ 11 , 12 ]. (hindawi.com)
- Magnetic resonance imaging (MRI) offers detailed characterization of the kidney structure without using ionizing radiation and is suitable for monitoring renal allograft damage with repeated scanning. (hindawi.com)
Transplant5
- Animals tolerized to a heart allograft with ATG rejected an orthotopic lung transplant from the same strain as the original allograft, whereas recipients treated with CsA at the time of their heart transplant accepted a subsequent lung graft. (northwestern.edu)
- Surprisingly, animals treated with either ATG or CsA that had rejected a lung allograft accepted a subsequent heart transplant. (northwestern.edu)
- His, mentor in the 1980s was Professor Jean Marquet, Antwerp, Belgium - the father of Tympano-meatal and tympano-ossicular allograft transplant surgery. (wikipedia.org)
- Chronic allograft damage (CAD), characterised by interstitial fibrosis and tubular atrophy (IFTA), is the commonest cause of transplant failure following surgery [ 1 ]. (hindawi.com)
- Collectively, these data confirmed that iPSC-derived MSC-mediated immunosuppression has potential to establish immune tolerance and rescue allograft from sustained hypoxic/ischemic phase, and subsequently limits long-term airway epithelial injury and collagen progression, which therapeutically warrant a study of Cymerus iPSC-derived MSCs as a potential management option for immunosuppression in transplant recipients. (biomedcentral.com)
Orthotopic6
- Improved therapeutic outcomes of thermal ablation on rat orthotopic liver allograft sarcoma models by radioiodinated hypericin induced necrosis targeted. (oncotarget.com)
- This study aimed to investigate the hypothesis that radioiodinated hypericin ( 131 I-Hyp) may inhibit residual tumor recurrence after microwave ablation (MWA) on rat orthotopic liver allograft sarcoma models. (oncotarget.com)
- In conclusion, 131 I-Hyp induced necrosis targeted radiotherapy improved therapeutic outcomes of MWA on rat orthotopic liver allograft sarcoma models. (oncotarget.com)
- The purpose of this study was to determine if pirfenidone was protective against the development of fibro-collagenous injury in rat lung orthotopic transplants through altering L-arginine-arginase metabolic pathways. (nih.gov)
- The orthotopic glioblastoma allograft was generated by intracerebral injection of rat C6 cells through a guide-cannula system and after 21 post-inoculation days the brain tumor was characterized by histoimmunopathological experiments. (elsevierpure.com)
- Here, we demonstrated the impact of iPSC-derived human MSCs on the development of immune tolerance and long-term graft survival in mouse orthotopic airway allografts. (biomedcentral.com)
Wistar2
- Isolated male Wistar rat lungs were perfused in a recirculatory model in the presence of 5-nmol/liter relaxin (n = 17) or vehicle alone (n = 14). (nih.gov)
- Twenty male Wistar rats were divided into 2 groups: Group 1 (rats treated with FK506) and Group 2(rats treated with saline solution vehicle). (bvsalud.org)
Dysfunction3
- However, chronic allograft dysfunction in the form of bronchiolitis obliterans remains a major hurdle that threatens both the quality of life and long-term survival of the recipients. (ersjournals.com)
- After successful reduction of early complications, chronic allograft dysfunction has become the major obstacle to long-term survival. (ersjournals.com)
- Pulmonary injury leading to early allograft dysfunction is caused by ischemia-reperfusion injury (IR) and originates from multiple pathogenic events, including endothelial damage, neutrophil extravasation into tissue, and peroxidation of cell membrane lipids, followed by pulmonary cell alterations and edema. (nih.gov)
Lung5
- We compared the histopathology of the airways in 3 groups of rats: (1) nontransplanted LEW lungs, (2) LEW-to-LEW syngeneic lung transplants, and (3) BN-to-LEW allogeneic lung transplants. (rug.nl)
- A significantly increased peak airway pressure (PawP) with excessive collagen deposition was found in untreated lung allografts. (nih.gov)
- Pirfenidone treatment decreased PawP and collagen content in lung allografts. (nih.gov)
- In addition, pirfenidone decreased endogenous transforming growth factor (TGF)-beta level in lung allografts, and TGF-beta stimulated arginase activity in a dose-dependent manner in both lung tissue and fibroblasts. (nih.gov)
- These results suggest that pirfenidone inhibits local arginase activity possibly through suppression of endogenous TGF-beta, hence, limiting the development of fibrosis in lung allografts. (nih.gov)
Therapeutic1
- Histological staining and immunofluorescent labelings for TERT, VEGF, Bcl-2, survivin, XIAP, and GFAP revealed the distinct characteristics of glioblastoma in C6 allograft, which could be useful as a target for treatment with emerging new therapeutic agents. (elsevierpure.com)
Survival2
- The induction of prolonged survival and growth of allografts of either tumors or normal tissues which would ordinarily be rejected. (umassmed.edu)
- Combination treatment of Tofacitinib with mycophenolate mofetil (MMF) significantly improved allograft survival in nonhuman primates compared to treatment with MMF alone. (lclabs.com)
Antigen3
- Donor-specific antigen and cyclosporine in rat islet allografts. (umassmed.edu)
- There was a rat model, and the target antigen was known in the rat. (medscape.com)
- Megalin was the rat antigen, which rats have in their glomerulus, but humans don't. (medscape.com)
Liver1
- Overexpression of both sEng and sFlt1 in pregnant rats develops nephrotic-range proteinuria, severe hypertension, biochemical evidence of HELLP ("H" for hemolysis, "EL" for elevated liver enzymes, and "LP" for low platelet count), and intrauterine growth restriction of the pups [ 19 , 20 ]. (hindawi.com)
Fibrosis2
- Rats injected with ISO exhibited severe interstitial cardiac fibrosis and perivascular fibrosis, decreased left ventricular ejection fraction, and increased NOX activity. (dovepress.com)
- RESULTS: Allografts that underwent the three sterilization methods presents similar weight gain, cortical thickness similar to control group, and less fibrosis than the control group. (bvsalud.org)
Histological2
- These rats were killed for histological investigation 4, 7, 21, and 56 days after infection. (rug.nl)
- These rats were killed for histological investigation 4, 7, 21, and 56 days after infection.In the lungs of the noninfected rats of the nontransplanted and syngeneically transplanted groups, airway changes were absent. (rug.nl)
Vascular1
- Current imaging techniques for monitoring allograft function involve the use of ultrasound to exclude ureteric obstruction or vascular compromise in the failing kidney. (hindawi.com)
Bone9
- In 1984 Frootko, working with James Triffitt (now Emeritus Professor of Bone Metabolism in Oxford), was able to demonstrate new bone formation in human demineralised allograft ossicles used to reconstruct the ossicular chain. (wikipedia.org)
- Massive Bone Allograft Reconstruction in High-Grade Osteosar. (lww.com)
- From 1986 to 1994, 112 bone allograft reconstructions were performed in patients with high-grade osteosarcoma in whom neoadjuvant chemotherapy was administered. (lww.com)
- Clinical use of massive bone allografts was reported by Lexter 14 in 1908 using fresh allografts retrieved from amputated limbs. (lww.com)
- In the authors' institute, massive frozen allografts have been used as a means of reconstruction after segmental resection for bone tumors since 1984. (lww.com)
- PURPOSE: To compare three sterilization methods (autoclave, gamma irradiation and ethylene oxide) over non demineralized lyophilized bone allografts. (bvsalud.org)
- METHODS: Bone allografts were implanted on paravertebral muscles of 21 rats. (bvsalud.org)
- CONCLUSION: The three sterilization methods cause similar effects on bone allografts regarding macroscopic and histomorphometric parameters. (bvsalud.org)
- To test the hypothesis that bone quality may affect the stability of anchorage devices implanted in a rat model. (bvsalud.org)
Cyclosporine1
- 5. Abdelhadi M, Ericzon BG, Hultenby K, Sjoden G, Reinholt FP, Nordenstrom J. Structural skeletal impairment induced by immunosuppressive therapy in rats: cyclosporine A vs tacrolimus. (bvsalud.org)
Airway1
- In the lungs of the noninfected rats of the nontransplanted and syngeneically transplanted groups, airway changes were absent. (rug.nl)
19871
- Unfortunately this and all other reconstructive procedures using allografts were abandoned in 1987 because of the potential risk of transmission of HIV and Creutzfeldt-Jakob disease from donor material to recipient. (wikipedia.org)
Tumor1
- Parrish 19 in 1966 and Mankin et al 15 in 1976 reported good results in 80% of allograft reconstruction performed primarily for tumor resections. (lww.com)
Murine1
- Tofacitinib was evaluated in murine collagen-induced (CIA) and rat adjuvant-induced (AA) models of rheumatoid arthritis (RA). (lclabs.com)
Organ1
- 1. The immunological mechanisms involved in sustaining normal semi-allogeneic pregnancies and in the enhancement of organ allografts were investigated in inbred rats. (portlandpress.com)
Hypoxic1
- Normoxic CM and hypoxic CM were isolated from rat BMSCs cultured under normoxic (20% O 2 ) or hypoxic (1% O 2 ) condition. (researchsquare.com)
Experimental1
- Mycophenolate mofetil also inhibited proliferative arteriopathy in experimental models of aortic and cardiac allografts in rats, as well as in primate cardiac xenografts. (nih.gov)
Underwent1
- From August 1986 to December 1994, 112 patients with high-grade osteosarcoma underwent preoperative chemotherapy, limb-sparing resection, reconstruction with a massive allograft, and postoperative chemotherapy. (lww.com)
Immunosuppressive2
- Early identification of chronic allograft damage remains challenging but is crucial to allow intervention with immunosuppressive therapy. (hindawi.com)
- Schwartz and Dameshek, in 1959, showed that 6-mercaptopurine was immunosuppressive in rats, ushering in the era of immunosuppressive drug treatment. (medscape.com)
Femurs1
- The allograft reconstruction was used in arthrodesis in 44 cases (41 knees, three ankles), as an intercalary graft in 39 (28 femurs, 11 tibias), as an osteoarticular graft in 22 (three proximal and/or distal humeri, six distal femurs, 13 proximal tibias), and as an allograft and prosthesis composite in seven (two proximal humeri, one proximal femurs, four proximal tibias). (lww.com)
Pathway1
- 3. Jörgen Henriksson and Hans Tjälve (2000) Manganese Taken Up into the CNS via the Olfactory Pathway in Rats Affects Astrocytes. (ihcworld.com)
Recombinant3
- 2. The antigenic targets for alloantibodies formed after leucocyte transfusions and multiple allogeneic pregnancies were defined by the EA rosette inhibition (EAI) assay in several congenic and recombinant inbred rat strains. (portlandpress.com)
- responses to the latter three antigens could not be separated with available congenic recombinant inbred rat strains. (portlandpress.com)
- Evidence for the involvement of sFlt1 in the occurrence of PE was initially provided by an animal model in which gravid rats were infected with a recombinant adenovirus encoding sFlt1 and compared to animals infected with the empty control adenovirus. (hindawi.com)
Inhibition1
- Janus kinase 3 inhibition with CP-690,550 prevents allograft vasculopathy. (lclabs.com)
Reconstruction1
- Debate continues 6,9,11,12,22,23 relative to the effectiveness of massive allograft reconstruction compared with other techniques (endoprostheses), particularly for patients receiving intensive chemotherapy. (lww.com)
Induce1
- Nontransplanted and transplanted rats were treated with CsA to induce permanent graft acceptance of the allografts. (rug.nl)
Model1
- Orthodontic mini-implants did not present good primary stability in a rat model with osteopenia. (bvsalud.org)
Functional1
- The Streblow lab in conjunction with Dr. Susan Orloff's laboratory at OHSU has determined RCMV in vivo gene expression in infected rats and has begun functional studies of these genes. (ohsu.edu)
Vivo1
- In Vivo Activity of Genetically Modified Cells Preseeded in Rat Vascularized Composite Allografts. (harvard.edu)
Effectively1
- In addition, TMZ effectively inhibited EndMT in ISO-treated rat hearts and TGF-β-treated HUVECs, as manifested by increased CD31 expression, decreased α-SMA expression, and suppressed cell migration. (dovepress.com)
Results1
- Ottolenghi 17,18 and Volkov, 21 reported successful results in approximately 75% of patients using anatomic specimen frozen allografts. (lww.com)