AlkBDegradation of alkanesHydroxylase systemAmmonia monooxygenasePseudomonasMethane monooxygenaseEnzymeCharacterizationSubunitOxidationDegradersGeneTranscriptionalCompounds2180Polysaccharide2023AlcoholsBiotransformationMicrobialOctanePredominantAbstractAbundanceMetabolismFattyComplexCarbon sourcesGenesCapabilitiesChainBiologicalTerminalPathwaysConversionHydrolaseOrganicAnalysis
AlkB4
- This was explored using 16S rRNA gene-based terminal restriction fragment length polymorphism, along with alkane hydroxylase (AlkB) and soluble di-iron monooxygenase (SDIMO) gene-based clone libraries. (int-res.com)
- Alkane monooxygenase (AlkB), a membrane-spanning metalloenzyme, converts straight chain alkanes to alcohols in the first step of the microbially-mediated degradation of alkanes, thereby playing a critical role in the global cycling of carbon and the bioremediation of oil. (stanford.edu)
- AlkB biodiversity is attributed to its ability to oxidize alkanes of various chain lengths, while individual AlkBs target a relatively narrow range. (stanford.edu)
- These findings provide insight into the catalytic mechanism of AlkB and shed light on its function in alkane-degrading microorganisms. (stanford.edu)
Hydroxylase system1
- As explained earlier the catalytic component of the alkane hydroxylase system is an integral membrane protein. (igem.org)
Ammonia monooxygenase1
- Interaction of Ammonia Monooxygenase from Nitrosomonas europaea with Alkanes, Alkenes, and Alkynes. (ncsu.edu)
Pseudomonas4
- Degenerate primers designed for PCR amplification of genes related to the binuclear-iron-containing alkane hydroxylase from Pseudomonas oleovorans were used to clone a related gene from strain CF8. (elsevierpure.com)
- A positive feedback mechanism controls expression of AlkS, the transcriptional regulator of the Pseudomonas oleovorans alkane degradation pathway. (igem.org)
- Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes. (igem.org)
- Product repression of alkane monooxygenase expression in Pseudomonas butanovora J Bacteriol, 188: 2586-2592. (ijabbr.com)
Methane monooxygenase3
- Methane monooxygenase (soluble). (cathdb.info)
- Crystal structures of the soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath) demonstrating geometrical variability at the dinuclear iron active site. (cathdb.info)
- Of manually curated genomes, 22 (18 are complete), ranging from 159 kilobase (kb) to 527 kb in length, were found to encode the pmoC gene, an enzymatically critical subunit of the particulate methane monooxygenase, the predominant methane oxidation catalyst in nature. (nature.com)
Enzyme4
- In enzymology, an alkane 1-monooxygenase (EC 1.14.15.3) is an enzyme that catalyzes the chemical reactions an alkane + reduced rubredoxin + O2 ⇌ {\displaystyle \rightleftharpoons } a primary alcohol + oxidized rubredoxin + H2O. (wikipedia.org)
- The systematic name of this enzyme class is alkane, reduced-rubredoxin:oxygen 1-oxidoreductase. (wikipedia.org)
- Less-stable activations were achieved if enzyme was incubated with Na2S2O4 under 1 kPa H2 or 101 kPa N2. (ncsu.edu)
- Enzyme Nomenclature, 1992) EC 1. (lookformedical.com)
Characterization2
- Of course, one of the first characterization experiments was to test growth of the E.coli strains carrying BBa_K398014 on alkanes. (igem.org)
- Characterization of a lytic polysaccharide monooxygenase from Aspergillus fumigatus shows functional variation among family AA11 fungal LPMOs. (cube-synergy.eu)
Subunit1
- This gene was tagged by insertion of the Tc1 transposon, and the wild-type gene was cloned by chromosomal walking 11 kb from ama-1, a cloned gene encoding the large subunit of RNA polymerase II. (ncsu.edu)
Oxidation3
- Two Distinct Monooxygenases for Alkane Oxidation in Nocardioides sp. (elsevierpure.com)
- These results indicate the presence of two distinct monooxygenases for alkane oxidation in Nocardioides sp. (elsevierpure.com)
- Dive into the research topics of 'Two Distinct Monooxygenases for Alkane Oxidation in Nocardioides sp. (elsevierpure.com)
Degraders1
- Environmental studies demonstrate the abundance of alkane degraders and have lead to the identification of many new species, including some that are (near) obligate alkanotrophs. (ijabbr.com)
Gene4
- Reverse transcription-PCR and Northern blot analysis showed that this gene encoding a binuclear-iron-containing alkanc hydroxylase was expressed in cells grown on alkanes above C 6 . (elsevierpure.com)
- We achieve this by investigating 1) Organismal response to perturbations in its environment, 2) Gene and metabolite function in situ, 3) Environmental applications of novel microbial chemistries. (wisc.edu)
- Bacteriophages (phages), viruses that infect and replicate within bacteria, are important in both natural and human microbiomes because they prey on bacterial hosts, mediate horizontal gene transfer, alter host metabolism and redistribute bacterially derived compounds via host cell lysis 1 . (nature.com)
- Soluble di-iron monooxygenase gene diversity in soils, sediments and ethene enrichments. (ijabbr.com)
Transcriptional1
- This review summarizes recent progress on the metabolism of n -alkanes and its transcriptional control in response to n -alkanes and to fatty acids in Y. lipolytica . (go.jp)
Compounds3
- CO is oxidized to CO(2), ammonia is oxidized to hydroxylamine, and some aromatic compounds and cyclic alkanes can also be hydroxylated, but more slowly. (cathdb.info)
- Alkanes are chemical compounds that consist only of hydrogen and carbon atoms and are bonded exclusively by single bonds without any cycles. (absoluteastronomy.com)
- A potent ability to assimilate hydrophobic compounds, including n -alkanes and fatty acids as carbon sources, is one of important characteristics of the yeast Yarrowia lipolytica , and has been studied for both basic microbiological interest and biotechnological applications. (go.jp)
21801
- 14 (1): 2180. (wikipedia.org)
Polysaccharide1
- The impact of reductants on the catalytic efficiency of a lytic polysaccharide monooxygenase and the special role of dehydroascorbic acid. (cube-synergy.eu)
20231
- 13(1): 19229, 2023 11 06. (bvsalud.org)
Alcohols2
- Using these values for the constituent blocks of TOPP results in a high blood:air partition coefficient for the terpene alcohols (approximately 147:1) and moderate values (2.7:1 to 5.1:1) for sulfur-containing constituents. (europa.eu)
- In the metabolism of n -alkanes, cytochromes P450ALK catalyze their initial hydroxylation to fatty alcohols, which are subsequently converted to fatty acids and utilized. (go.jp)
Biotransformation1
- An option which has been explored in literature [1] is the resting-cell assay a.k.a. biotransformation assay. (igem.org)
Microbial1
- Alkane hydroxylases involved in microbial alkane degradation Appl Microbiol Biotechnol, 74: 13-21. (ijabbr.com)
Octane3
- The alkanes octane and dodecane were tested as possible substrates in M9 minimal medium. (igem.org)
- Growth on butane and hexane was strongly inhibited by 1-hexyne, while 1-hexyne did not affect growth on octane or decane. (elsevierpure.com)
- A specific 30-kDa acetylene-binding polypeptide was observed for butane-, hexane-, octane-, and decane-grown cells but was absent from cells grown with octane or decane in the presence of 1-hexyne. (elsevierpure.com)
Predominant1
- The transcription of ALK1 , encoding a predominant cytochrome P450ALK, is regulated in response to n -alkanes by two basic helix-loop-helix transcription activators, Yas1p and Yas2p, and Opi1-family transcription repressor Yas3p. (go.jp)
Abstract1
- abstract = "Alkane monooxygenases in Nocardioides sp. (elsevierpure.com)
Abundance1
- A 146 base pair sequence, encoding amino acids 56-103, is unique in the C. elegans genome, and it hybridizes to a 1 kb mRNA of moderate abundance. (ncsu.edu)
Metabolism1
- Potential projects focused on this theme are (1) investigating the contributions of metabolism to prognosis in tick-borne diseases like Lyme's, and (2) the effect of metal contamination on sulfur metabolism of the gut microbiome. (wisc.edu)
Fatty1
- In higher plants, the microsomal oleate Δ12 -desaturase (fatty acid desaturase 2, FAD2) is a hydrophobic endoplasmic reticulum protein, catalyzing the reaction from monounsaturated oleic acid (C18:1) to polyunsaturated linoleic acid (C18:2) by introducing a double bond between the 12th and 13th carbon atoms of C18:1 [1]. (researchsquare.com)
Complex2
- Crystal Structure of Long-Chain Alkane Monooxygenase (LadA) in Complex with Coenzyme FMN: Unveiling the Long-Chain Alkane Hydroxylase. (igem.org)
- As described in detail in Section 1 of the registration substance, Turpentine Oil from Pulping Processes (TOPP) is a complex UVCB substance containing a range of constituent types. (europa.eu)
Carbon sources1
- We use genomics, enzymology and metabolomics-based technologies to determine the roles for specific genes, enzymes and metabolites in different conditions such as (1) bioplastic-precursor synthesis from waste carbon sources, (2) degradation of recalcitrant plastics in the environment, and (3) the ecology of the interactions between microplastics and Harmful Algal Blooms. (wisc.edu)
Genes1
- G. hirsutum contains four pairs of homoeologous FAD2 genes (GhFAD2-1 to GhFAD2-4). (researchsquare.com)
Capabilities2
- The logic behind this is to stall the growth of a large volume of cells by using nitrogen-deficient medium to test their alkane conversion capabilities at near-zero growth. (igem.org)
- Initial efforts in this theme are focused on the electron transfer and bioinorganic synthetic capabilities of anaerobes towards (1) enhanced wastewater treatment and (2) electricity production in photobioelectrochemical calls. (wisc.edu)
Chain2
- The idea behind this is that E.coli might inherently contain an ADH and ALDH that, while it might be at an extremely low activity, can be able to degrade larger chain alkanes thus releasing energy for growth. (igem.org)
- Strain CF8 can utilize alkanes ranging in chain length from C 2 , to C 16 . (elsevierpure.com)
Biological1
- S pilled oil is transported, and its composition and character altered, by a variety of physical, chemical, and biological processes ( Figure 4-1 ). (nationalacademies.org)
Terminal1
- Methionine aminopeptidase 1 (MtMET-AP1) is a hydrolase that mediates the necessary post-translational N-terminal methionine excision (NME) of peptides during protein synthesis, which is necessary for bacterial proliferation and is a potential target for the treatment of tuberculosis. (bvsalud.org)
Pathways1
- Besides many tiny cavities, this empty internal spa`ce may form cavities of specific functions, as well as tunnels and channels (or pores), representing potential transport pathways for small molecules, ions and water molecules [1] . (plos.org)
Hydrolase1
- The proteins LTA4H (leukotriene A-4 hydrolase), METAP1 (methionine aminopeptidase 1), BID (BH3-interacting domain death agonist), SMAD1 (mothers against decapentaplegic homologue), PRKRA (interferon-inducible double-stranded RNA-dependent protein kinase activator A), YES1 (tyrosine-protein kinase Yes) and EGFL7 (epidermal growth factor-like protein 7) were significantly upregulated in EGFRI-stimulated peripheral blood mononuclear cell cultures, and validated in the lesions. (bvsalud.org)
Organic2
Analysis1
- Extraction hydrocarbons from the medium using an apolar solvent (such as ethyl acetate) after the reaction and subsequent analysis by gas chromatography would indicate the presence of the corresponding alkanol and/or the decrease of alkane. (igem.org)