Alkaline phosphatase activity. Medical search. Frequent questions

Multilocus phylogenetic analysis showed the emia, increased serum alkaline phosphatase activity, dogs were infected with a novel Rickettsia species related hypoglycemia, and hyponatremia (Table 1). (cdc.gov)
Idiopathic retroperitoneal fibrosis--is serum alkaline phosphatase a marker of disease activity? (medscape.com)
They may also contribute to the circulating pool of serum alkaline phosphatase under specific situations when there is increased stimulation of their production. (statpearls.com)
Serum alkaline phosphatase levels will vary with age in normal individuals. (statpearls.com)
[8] In individuals with blood groups O and B, serum alkaline phosphatase levels increase after consuming a fatty meal, due to contribution from the intestinal tract. (statpearls.com)
Measurement of serum alkaline phosphatase-in some cases, bone-specific alkaline phosphatase (BSAP)-can be useful in the diagnosis of Paget disease. (medscape.com)

Recombinant SEAP (secreted embryonic alkaline phosphatase) protein is a truncated form of human placental alkaline phosphatase that comprises 520 amino acids. (invivogen.com)
SPECIFICITY: Human placental alkaline phosphatase (Regan and Nagao isoenzymes). (researchd.com)
Intestinal alkaline phosphatase is coded by a separate gene, which is different from the gene that codes for placental alkaline phosphatase and the Regan isoenzyme (produced in excess amounts in Hodgkin lymphoma). (statpearls.com)

This is a result of additional alkaline phosphatase produced by the placenta and the liver. (wikipedia.org)
Additionally, abnormal levels of alkaline phosphatase in the blood could indicate issues relating to the liver, gall bladder or bones. (wikipedia.org)
In adults with normal liver function, approximately 50% of the total alkaline phosphatase activity arises from the liver and 50% from the bone. (medscape.com)
Alkaline phosphatases are a group of enzymes found primarily the liver and bone. (essense-of-life.com)
The primary importance of measuring alkaline phosphatase is to check the possibility of bone disease or liver disease. (essense-of-life.com)
In the liver, alkaline phosphatase is cytosolic and present in the canalicular membrane of the hepatocyte. (statpearls.com)
[1] Alkaline phosphatase is present in decreasing concentrations in the placenta, ileal mucosa, kidney, bone, and liver. (statpearls.com)
The majority of alkaline phosphatase in serum (more than 80%) is released from the liver and bone, and in small amounts from the intestine. (statpearls.com)
Lysosomal enzyme activities did not change in the liver tissue. (portlandpress.com)
5. Liver catalase and d-amino acid oxidase activities showed a slight increase at 1 day post-ligation but a significant fall by 8 days. (portlandpress.com)
Measuring total alkaline phosphatase levels may be useful in patients with normal liver function. (medscape.com)
In patients with abnormal liver function or other causes of elevated alkaline phosphatase activity not due to bone, BSAP is a reasonable means of assessing Paget disease activity. (medscape.com)
The highest concentration is in the kidney, but the liver is considered the source of normal enzyme activity. (medscape.com)

In order to examine the consequences of CD26/DPP IV deficiency on the activity of other enzymes, CD26 deficient mice were investigated. (srce.hr)
The relationship between ageing and brush-border membrane enzymes activities was characterized in different small intestinal segments (duodenum, jejunum, ileum) in mice aged 2 weeks, 1, 2, 3, 6 and 12 months. (srce.hr)
The overall goal of this project was to use the tools of physical organic chemistry to probe the mechanistic features of promiscuous enzymes in the alkaline phosphatase superfamily, a structurally related family of enzymes that appears to be particularly prone to promiscuous behaviour. (europa.eu)
2005). Carbohydrate metabolizing enzymes (e.g., α-amylase), lipid degrading enzymes (e.g., lipase) and phosphatases (acid and alkaline phosphatases) were recorded among target enzymes which should be disturbed and may provide more accurate information on the molluscicide-induced stress on molluscs. (scialert.net)
7. Serum activities of mitochondrial, plasma membrane, microsomal, lysosomal and cytosol marker enzymes tended to increase post-ligation, particularly at 8 days. (portlandpress.com)
There were many studies that monitor progress of tooth movement using enzymes e.g., alkaline phosphatase (ALP) (Perinetti et al . (scialert.net)
The purpose of this study was to investigate the activities of the total acid phosphatase (TAP), tartrate-resistant acid phosphatase (TRAP), low molecular weight protein tyrosine phosphatase (LMW-PTP) and alkaline phosphatase (ALP) enzymes, as well as the possible correlation in the serum and in unstimulated whole saliva of children. (bvsalud.org)
The enzymatic activities of all the studied enzymes were higher in serum than in saliva. (bvsalud.org)

The profile of intestinal brush-border membrane enzyme activity is described in several mice and rat species. (srce.hr)
The aim of this study was to determine the influence of age on intestinal brush-border disaccharidases (lactase, sucrase, and maltase), and alkaline phosphatase activity in CD26 deficient mice. (srce.hr)
The results of this study revealed that intestinal enzyme activities change statistically significantly with ageing in CD26 deficient mice. (srce.hr)
Infection may result in decreased intestinal absorption of sodium, glucose, and water, and decreased levels of intestinal lactase, alkaline phosphatase, and sucrase activity, and may lead to isotonic diarrhea. (cdc.gov)

This marker is designed to distinguish leukocytes and determine different enzyme activity from each sample's extent of staining. (wikipedia.org)
To assist in the evaluation of skeletal status, one marker of bone turnover is being measured: bone alkaline phosphatase in serum. (cdc.gov)
Alkaline phosphatase has been clinically available for several years as a marker for bone metabolism. (medscape.com)
This study investigated the potential of AST as a biological marker to monitor tooth movement by determining its activity in Gingival Crevicular Fluid (GCF) during bodily tooth movement (canine distalization). (scialert.net)

In the present study, glucose, acid and alkaline phosphatases (ACP and ALP), α-amylase and lipase were measured for the first time in tissue homogenates of Biomphalaria arabica snails, molluscan intermediate host for Schistosoma mansoni in Saudi Arabia. (scialert.net)
Glucose 6-phosphatase activity and ribonucleic acid, which are localized predominantly in the rough endoplasmic reticulum, were increased. (portlandpress.com)

GDF-5 also increased the gene expression and enzyme activity of alkaline phosphatase. (ispub.com)
Identical Rickettsia DNA gene albuminemia, increased alanine amino transferase sequences were obtained from each dog's blood spec- activity, alkaline phosphatase activity, hypercholes- imen and used to investigate Rickettsia spp. (cdc.gov)
The ALPL gene provides instructions for making an enzyme called tissue-nonspecific alkaline phosphatase (TNSALP). (medlineplus.gov)
Brun-Heath I, Taillandier A, Serre JL, Mornet E. Characterization of 11 novel mutations in the tissue non-specific alkaline phosphatase gene responsible for hypophosphatasia and genotype-phenotype correlations. (medlineplus.gov)
Mornet E. Hypophosphatasia: the mutations in the tissue-nonspecific alkaline phosphatase gene. (medlineplus.gov)

The Tandem-MP Ostase ImmunoEnzymetric Assay is an in vitro device for the quantitative measurement of Skeletal Alkaline Phosphatase (sALP), an indicator of osteoblastic activity, in human serum. (cdc.gov)
2008), where activity of osteoblastic cells was related to secretion of ALP during bone formation (Intan et al . (scialert.net)
Because of increased osteoblastic activity and bone formation, bone-specific alkaline phosphatase (BSAP) levels are elevated. (medscape.com)

10 μg lyophilized recombinant human secreted embryonic alkaline phosphatase (SEAP). (invivogen.com)
A mixed population of neuronal and glial restricted precursors (NRP/GRP) derived from the embryonic spinal cord of alkaline phosphatase (AP) transgenic rats were grafted acutely into a DC lesion at C1. (jneurosci.org)

A growing body of data suggests that the loss of tissue nonspecific alkaline phosphatase (TNAP) enzyme activity in cerebral microvessels may be associated with changes in endothelial cell barrier integrity. (biorxiv.org)

The development of immunoassay-based markers with monoclonal antibodies directed to the bone-specific isoform of alkaline phosphatase has improved specificity and sensitivity. (medscape.com)

The effect of Cr on ALP activity was more pronounced at higher concentrations of Cr, but 20 mM Cr already showed some adverse effects on cell viability. (nih.gov)
Low serum ALP activity relates to reduced zinc, magnesium and manganese concentrations. (essense-of-life.com)
In the original intensive-treatment arm of the PRISM study, the goal was to suppress bone turnover by reducing and maintaining serum total alkaline phosphatase (ALP) concentrations within the normal range, as the investigators explain. (medscape.com)

Alkaline phosphatase in E. coli is uncommonly soluble and active within elevated temperature conditions such as 80 °C. Due to the kinetic energy induced by this temperature the weak hydrogen bonds and hydrophobic interactions of common proteins become degraded and therefore coalesce and precipitate. (wikipedia.org)
To investigate the antibacterial activities of aqueous and methanolic extracts, as well as, partially purified proteins extracted from Terfezia claveryi aqueous, against Pseudomonas aeruginosa (P. aeruginosa). (researchgate.net)
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins. (sigmaaldrich.com)

2003). A previous in-vitro study on the role of GDF-5 in periodontal ligament cells has shown that exogenous GDF-5 promotes cell proliferation while suppressing the activity of alkaline phosphatase (ALP), which is closely related to tissue calcification and which is secreted during the formation of tissue calcifications. (ispub.com)
Alkaline phosphatases are classified as tissue-specific and tissue-nonspecific types. (statpearls.com)
Alkaline phosphatases found in the intestine, placenta, and germinal tissue are tissue-specific. (statpearls.com)
The tissue-nonspecific alkaline phosphatases form most of the fraction circulating in serum and, therefore, are of clinical interest. (statpearls.com)
6. Lactate dehydrogenase, a cytosol enzyme, showed a decrease in activity after 1 day but an increase in tissue activities 8 days after ligation. (portlandpress.com)
Structural evidence for a functional role of human tissue nonspecific alkaline phosphatase in bone mineralization. (medlineplus.gov)

Serum total acid phosphatase is an osteoclastic enzyme that may be elevated in active Paget disease. (medscape.com)
In males, however, the clinical value of this finding is compromised, as acid phosphatase levels also may be elevated in the presence of metastatic prostate carcinoma. (medscape.com)

Sucrase will have the greatest activity at pH 6 2. (ipl.org)
Sucrase will have the greatest activity at 50 °C (122 °F) 3. (ipl.org)
Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. (ipl.org)

abstract = "Alkaline phosphatase (ALP), an ectoenzyme, plays important roles in biology. (elsevierpure.com)

The pattern of activity of aspartate aminotransferase enzyme (AST) following canine distalization stage in orthodontic treatment is being investigated. (scialert.net)
The current study which investigated the pattern of aspartate aminotransferase (AST) activity following application of force to distalize the canine (100 g) was a continuation of an earlier study by the same researcher which investigated the enzyme`s activity after the application of force to align teeth (30-60 g). (scialert.net)

Alkaline phosphatase is a zinc-containing dimeric enzyme with the MW: 86,000 Da, each subunit containing 429 amino acids with four cysteine residues linking the two subunits. (wikipedia.org)

Although alkaline phosphatases are present in different body tissues and have different physiochemical properties, they are true isoenzymes because they catalyze the same reaction. (statpearls.com)

A strong relationship exists between the extent of disease activity measured by scintigraphy and the degree of the elevation of alkaline phosphatase in persons with untreated Paget disease. (medscape.com)
The major clinical value of serum GGT is in evaluating patients with isolated elevation in alkaline phosphatase because differentiating between hepatobiliary and bone causes in these cases can be difficult. (medscape.com)

As such, the main purpose of dephosphorylation by alkaline phosphatase is to increase the rate of diffusion of phosphorylated molecules into the cells while inhibiting them from diffusing out. (wikipedia.org)

Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. (medlineplus.gov)

The known activities of PMH include the hydrolysis of phosphate mono-, di-, and triesters (1,2,3 respectively), sulfate monoesters (5), and sulfonate monoesters (6) in addition to its native activity towards the hydrolysis of phosphonate monoesters (4). (europa.eu)
This enzyme acts as a phosphatase, which means that it removes clusters of oxygen and phosphorus atoms (phosphate groups) from other molecules. (medlineplus.gov)
Low phosphate and high alkaline phosphatase levels characterize most of the disorders. (medscape.com)

The degree of differentiation of primary osteoblast-like cell cultures was higher in micromass cultures compared to monolayer cultures, as judged by alkaline phosphatase (ALP) activity and extent of mineralization. (nih.gov)
In both culture systems, Cr supplementation showed positive effects, which were dependent on the organizational level of the osteoblast-like cells in such a way that the cells in monolayer culture showed significantly increased metabolic activity, ALP activity and mineralization in the presence of Cr than without the supplement. (nih.gov)
In micromass cultures, Cr also significantly enhanced ALP activity and mineralization, without affecting metabolic activity. (nih.gov)
In conclusion, chemically pure Cr added to low serum cell culture medium has a stimulatory effect on metabolic activity, differentiation and mineralization of osteoblast-like cells indicating that Cr supplementation could also be used as a potential clinical intervention to stimulate cell growth, differentiation and mineralization during bone repair in vivo. (nih.gov)

After pellet protein data were standardized on the basis of DNA content, only ALP activity of MSCs treated with FGF-2-dexamethasone remained significantly increased. (avma.org)

ALP activity improves when these nutrients are supplemented, both in vitro and in vivo. (essense-of-life.com)
Preliminary In Vitro and In Vivo Evaluation of Antidiabetic Activity of Ducrosia anethifolia Boiss. (hindawi.com)
Preliminary in vitro evaluation of the antidiabetic activity of crude extract and its furanocoumarins was carried out ( α -amylase, α -glucosidase, and β -galactosidase). (hindawi.com)
The decrease in electrophoretic mobility and activation of PKB induced by insulin was reversed both in vitro by treatment of the enzyme with alkaline phosphatase and in the intact adipocyte upon removal of insulin or addition of the phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor wortmannin. (lu.se)

The enzyme alkaline phosphatase (ALP, alkaline phenyl phosphatase) has the physiological role of dephosphorylating compounds. (wikipedia.org)
Even though alkaline phosphatases are present in many tissues throughout the body, their precise physiological function remains largely unknown. (statpearls.com)

Kidney tumors and infections as well as malnutrition have also shown abnormal level of alkaline phosphatase in blood. (wikipedia.org)
Although a viral cause remains controversial, it is hypothesized that in genetically predisposed patients an as yet unidentified virus triggers abnormal osteoclast activity. (msdmanuals.com)

This document specifies a fluorimetric microplate method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) [ 5 ] activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk, cream, flavoured milks and cheeses. (iso.org)
IDF 209: 2007 - Milk and milk-based drinks - Determination of alkaline phosphatase activ. (fil-idf.org)
This standard specifies a method for the determination of the alkaline phosphatase activity in pasteurized who. (fil-idf.org)

Since the periplasmic gap is more prone to environmental variation than the inner cell, alkaline phosphatase is suitably resistant to inactivation, denaturation, or degradation. (wikipedia.org)

Sections 12 to 32 micra thick were incubated in a substrate solution for the histochemical demonstration of non-specific alkaline phosphatase activity. (rupress.org)
The mechanism of action of alkaline phosphatase involves the geometric coordination of the substrate between the Zn ions in the active sites, whereas the Mg site doesn't appear to be close enough to directly partake in the hydrolysis mechanism, however, it may contribute to the shape of the electrostatic potential around the active center. (wikipedia.org)

Here, we report the profiling of the activities of ALPs on live cells by enzyme-instructed self-assembly (EISA) of a D-peptidic derivative that forms fluorescent, non-diffusive nanofibrils. (elsevierpure.com)
Evaluating Alkaline Phosphatase-Instructed Self-Assembly of d-Peptides for Selectively Inhibiting Ovarian Cancer Cells. (bvsalud.org)

It makes sense that osteoblasts by creating a local environment of alkalinity via alkaline phosphatase helps build bone. (essense-of-life.com)
Serum osteocalcin, which is produced specifically by osteoblasts, does not reflect disease activity. (medscape.com)

But there is no reliable activity probe for imaging ALPs on live cells because of the diffusion and cytotoxicity of current probes. (elsevierpure.com)
Our study reveals significantly higher activity of ALP on cancer cells than on stromal cells in co-cultures and shows an inherent and dynamic difference in ALP activity between drug-sensitive and drug-resistant cancer cells and between cancer cells with and without hormonal stimulation. (elsevierpure.com)
Being complementary to genomic profiling of cells, EISA, as a reaction-diffusion controlled process, achieves high spatiotemporal resolution for profiling the activity of ALPs on live cells at the single-cell level. (elsevierpure.com)
This study investigates new EISA substrates of alkaline phosphatase (ALP) to hinder ovarian cancer cells . (bvsalud.org)

The in vivo activity was investigated by measuring some oxidative stress markers. (hindawi.com)

Blood levels of alkaline phosphatase also increase by two to four times during pregnancy. (wikipedia.org)
Urinary hydroxyproline levels are elevated in Paget disease, as a reflection of increased osteoclastic activity and bone resorption. (medscape.com)

Ducrosia species were investigated mainly for their oil analysis and their biological activities [ 6 ]. (hindawi.com)

The compounds showed inhibitory activity in a concentration dependant manner. (hindawi.com)
Chez les femmes préménopausées et postménopausées, la concentration sérique en 25-OHD, en phosphore et en calcium était stable dans la fourchette d'âge. (who.int)

However, BSAP is more specific than total alkaline phosphatase for Paget disease. (medscape.com)
In patients with monostotic disease or local disease, the total alkaline phosphatase level may be normal. (medscape.com)

Serum bone alkaline phosphatase (ALP) activity is a measure of bone formation. (essense-of-life.com)
Measure and compare the activity of proteases with this non-specific protease activity assay using casein. (sigmaaldrich.com)

Brønsted analysis of seven active site mutants showed that the mutations cause only modest changes in βLG for all of the promiscuous activities. (europa.eu)
ALPL mutations that almost completely eliminate the activity of TNSALP usually result in the more severe forms of hypophosphatasia. (medlineplus.gov)
Other mutations, which reduce but do not eliminate the activity of the enzyme, are often responsible for milder forms of the condition. (medlineplus.gov)
Several other mutations associated with Paget disease have been identified, many affecting the RANK (receptor activator of nuclear factor kappa-B) signaling pathway that is critical for osteoclast generation and activity. (msdmanuals.com)

The human alkaline phosphatases (hALP) are found anchored on the cell membrane by glycosylphosphatidylinositol. (statpearls.com)

Enzymatic activities were measured in pairs of concurrently obtained serum and salivary samples from 32 children in good oral and systemic health (16 of each sex) with a median age of 6.4 ± 3.3 years (range 1.08 - 12.92 years). (bvsalud.org)

In Gram-negative bacteria, such as Escherichia coli, alkaline phosphatase is located in the periplasmic space, external to the inner cell membrane and within the peptidoglycan portion of the cell wall. (wikipedia.org)
3. The plasma membrane enzyme, alkaline phosphatase, increased in activity after bile-duct ligation. (portlandpress.com)

Other activities carried out by the IPCS include the development of know-how for coping with chemical accidents, coordination of laboratory testing and epidemiological studies, and promotion of research on the mechanisms of the biological action of chemicals. (inchem.org)

8. Monoamine oxidase, a predominantly mitochondrial enzyme, was greatly elevated in the serum after 1 day but had returned to normal activities by 8 days. (portlandpress.com)
Consequently, a normal alkaline phosphatase level does not exclude the disorder. (medscape.com)

However, the structure and activity correlation of EISA remains to be explored. (bvsalud.org)

The enzymatic activity described may be part of the mechanism regulating the blood flow. (rupress.org)

4. No changes in mitochondrial enzyme activities were noted after 1 day but there was a 50% reduction 8 days after ligation. (portlandpress.com)

Anatomy38

Organisms7

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Chemicals and Drugs126

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes48

Disciplines and Occupations2

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Serum alkaline6

Placental alkaline phosphatase3

Liver13

Enzymes8

Intestinal4

Marker4

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TNAP1

Specificity1

Concentrations3

Proteins3

Tissue6

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Elevation2

Phosphorylated molecules1

Skeletal1

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Mineralization4

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Vitro4

Physiological2

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Determination3

DEGRADATION1

Substrate2

Self-Assembly2

Osteoblasts2

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Enzyme activities1