This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. (cancerindex.org)
Human aldose3
The active site pocket of human aldose reductase is relatively hydrophobic, lined by seven aromatic and four other non-polar residues. (wikipedia.org)
In vitro inhibition of recombinant human aldose reductase expressed in E coli . (guidetopharmacology.org)
2015) The Effect of Halogen-to-Hydrogen Bond Substitution on Human Aldose Reductase Inhibition. (guidetopharmacology.org)
Antibody3
Western Blot analysis of Hela cells and 293T cells using AKR1B1 Polyclonal Antibody at dilution of 1:350. (elabscience.com)
Immunohistochemistry of paraffin-embedded Human lung cancer using AKR1B1 Polyclonal Antibody at dilution of 1:40. (elabscience.com)
2. According to the recommended primary antibody dilution ratio, use the TBST Buffer containing 5% Skim Milk Powder to dilute the AKR1B1 Antibody at , soak the PVDF Membrane in the primary antibody working solution, incubate overnight at 4 ℃, and gently shake. (elabscience.com)
Ketones2
It is a reduced nicotinamide-adenine dinucleotide phosphate (NADPH)-dependent enzyme catalyzing the reduction of various aldehydes and ketones to the corresponding alcohol. (wikipedia.org)
These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. (cancerindex.org)
Inhibitors1
2005) Discovery of 3-[(4,5,7-trifluorobenzothiazol-2-yl)methyl]indole-N-acetic acid (lidorestat) and congeners as highly potent and selective inhibitors of aldose reductase for treatment of chronic diabetic complications. (guidetopharmacology.org)
Gene2
Aldo-keto reductase family 1, member B1 (AKR1B1), also known as aldose reductase, is an enzyme that is encoded by the AKR1B1 gene in humans. (wikipedia.org)
The AKR1B1 gene lies on the chromosome location of 7q33 and consists of 10 exons. (wikipedia.org)
Family2
1.-.-.- Oxidoreductases: aldo-keto reductase family 1 member B. Last modified on 13/08/2015. (guidetopharmacology.org)
Aldo-keto reductase family 1 member B10 (AKR1B10) is an inflammatory regulator and is closely related to the prognosis of patients with GC. (portlandpress.com)
Consists1
AKR1B1 consists of 316 amino acid residues and weighs 35853Da. (wikipedia.org)
Process1
The involvement of AKR1B1 in oxidative stress diseases, cell signal transduction, and cell proliferation process endows AKR1B1 with potential as a therapeutic target. (wikipedia.org)
Structure2
3. Singh SB, Malamas MS, Hohman TC, Nilakantan R, Carper DA, Kitchen D. (2000) Molecular modeling of the aldose reductase-inhibitor complex based on the X-ray crystal structure and studies with single-site-directed mutants. (guidetopharmacology.org)
4. Steuber H, Zentgraf M, Gerlach C, Sotriffer CA, Heine A, Klebe G. (2006) Expect the unexpected or caveat for drug designers: multiple structure determinations using aldose reductase crystals treated under varying soaking and co-crystallisation conditions. (guidetopharmacology.org)
Aldose reductase10
Aldo-keto reductase family 1, member B1 (AKR1B1), also known as aldose reductase, is an enzyme that is encoded by the AKR1B1 gene in humans. (wikipedia.org)
The active site pocket of human aldose reductase is relatively hydrophobic, lined by seven aromatic and four other non-polar residues. (wikipedia.org)
For detailed information about aldose reductase, go to the full flat file . (brenda-enzymes.info)
The mouse Akr1b3 gene is clearly orthologous to human AKR1B1, both coding for aldose reductase, and their gene products show similar tissue distribution, regulation by osmotic stress and kinetic properties. (bvsalud.org)
17. Molecular Interactions and Implications of Aldose Reductase Inhibition by PGA1 and Clinically Used Prostaglandins. (nih.gov)
AT-001 (Caficrestat) is a highly potent, selective inhibitor of aldose reductase enzymatic activity with IC50 of 28.9 pM. (probechem.com)
A noncompetitive and reversible aldose reductase inhibitor with IC50 of 10 nM and 26 nM for purified aldose reductase from rat lens and human placenta, respectively. (probechem.com)
Imirestat (AL-1576) is a potent aldose reductase inhibitor with IC50 of 8.5 nM for rat lens aldose reductase, with similar IC50 value for the human placental enzyme, shows high affinity for AKR1A1 and AKR1B1 with Ki of 47 and 46 nM respectively. (probechem.com)
Govorestat (AT-007) is a potent, selective, CNS-penetrant aldose reductase (aldehyde reductase) inhibitor with IC50 of 0.1 nM, reduces toxic galactitol levels and prevents galactosemia disease complications in vivo. (probechem.com)
IDD388 is a potent aldose reductase (ALR2 or AKR1B1) inhibitor with IC50 of 0.4 uM, shows weak inhibition for AKR1B10 (IC50=4.4 uM). (probechem.com)
AKR1C32
14. Proteasome inhibitors MG-132 and bortezomib induce AKR1C1, AKR1C3, AKR1B1, and AKR1B10 in human colon cancer cell lines SW-480 and HT-29. (nih.gov)
BAY1128688 is a potent, steroidal aldo-keto reductase 1C3 (AKR1C3) inhibitor with potential for endometriosis. (probechem.com)
Gene3
The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. (nih.gov)
The AKR1B1 gene lies on the chromosome location of 7q33 and consists of 10 exons. (wikipedia.org)
4. Evaluation of an aldo-keto reductase gene signature with prognostic significance in colon cancer via activation of epithelial to mesenchymal transition and the p70S6K pathway. (nih.gov)
AKR1B105
There are 15 human AKRs of these AKR1B1, AKR1C1-1C3, AKR1D1, and AKR1B10 have been implicated in diabetic complications, steroid hormone dependent malignancies, bile acid deficiency and defects in retinoic acid signaling, respectively. (nih.gov)
10. Opposing roles of the aldo-keto reductases AKR1B1 and AKR1B10 in colorectal cancer. (nih.gov)
13. Overexpression and enhanced specific activity of aldoketo reductases (AKR1B1 & AKR1B10) in human breast cancers. (nih.gov)
16. AKR1B1 and AKR1B10 as Prognostic Biomarkers of Endometrioid Endometrial Carcinomas. (nih.gov)
MK-204 is a potent, selective AKR1B10 inhibitor with IC50 of 80 nM, shows high selective (270-fold) over AKR1B1. (probechem.com)
Protein1
There is conservation of the catalytic mechanism with short-chain dehydrogenases/reductases (SDRs) even though they show different protein folds. (nih.gov)
Regulates the expression1
7. β2-AR regulates the expression of AKR1B1 in human pancreatic cancer cells and promotes their proliferation via the ERK1/2 pathway. (nih.gov)
Cell proliferation1
The involvement of AKR1B1 in oxidative stress diseases, cell signal transduction, and cell proliferation process endows AKR1B1 with potential as a therapeutic target. (wikipedia.org)
AKRs2
The aldo-keto reductases (AKRs): Overview. (nih.gov)
Aldo-keto reductases (AKRs) are distributed in three families and multiple subfamilies in mammals. (bvsalud.org)
Lipid peroxidation1
It also participates in glucose metabolism and osmoregulation and plays a protective role against toxic aldehydes derived from lipid peroxidation and steroidogenesis. (wikipedia.org)
Inhibitor1
5. The AKR1B1 inhibitor epalrestat suppresses the progression of cervical cancer. (nih.gov)
Identification of dicarbonyl and L - xylulose reductase as a therapeutic target in human chronic kidney disease. (nih.gov)
Glucose1
This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. (nih.gov)
Enzyme1
The best substrate for the enzyme was pyridine-3-aldehyde. (bvsalud.org)
Mechanism1
20. Aldo-keto reductases-mediated cytotoxicity of 2-deoxyglucose: A novel anticancer mechanism. (nih.gov)
Breast1
11. AKR1B1 promotes basal-like breast cancer progression by a positive feedback loop that activates the EMT program. (nih.gov)
Potential2
1. Role of aldo-keto reductase family 1 member B1 (AKR1B1) in the cancer process and its therapeutic potential. (nih.gov)
19. A Potential Prognostic Biomarker for Glioma: Aldo-Keto Reductase Family 1 Member B1. (nih.gov)
Role1
18. Bioinformatics Analysis Reveals the Vital Role of AKR1B1 in Immune Infiltration and Clinical Outcomes of Gastric Cancer. (nih.gov)