• Alcian blue pH 2.5 - for the detection of acidic mucosubstances - for microscopy, is a ready-to-use staining kit used to stain acidic mucopolysaccharides in human-histological specimen materials, for example histological sections of e. g. the intestine or stomach. (sigmaaldrich.com)
  • but this stain is not typically used to identify goblet cells, and Alcian blue is preferred. (nih.gov)
  • The vacuoles stain blue with Mason�s trichrome stain. (nih.gov)
  • To determine the properties of the secretions of the ceruminous glands in the meatus acusticus externus of cats and dogs, the periodic acid Schiff (PAS) stain was used for neutral mucosubstances and glycogen, while the Alcian blue stain (pH 2.5) was applied for the presence of acidic mucosubstances. (tubitak.gov.tr)
  • The Alcian blue stain (pH 2.5) for acidic mucosubstances gave positive reactions for cats and negative reactions for dogs. (tubitak.gov.tr)
  • Amyloid deposits stain pink with hematoxylin and eosin, contain carbohydrate constituents that stain with periodic acid-Schiff dye or with Alcian blue, but most characteristically have apple-green birefringence under polarized light microscopy after Congo red staining. (msdmanuals.com)
  • Alcian blue stains acid mucosubstances and acetic mucins in paraffin-embedded tissue section. (pantomics.com)
  • Enables differentiation between acid mucins (stained light blue) and neutral mucins, glycogens and glycoproteins (stained red). (biognost.com)
  • Combined with Alcian Blue pH 2.5 solution on a single section enables differentiation between neutral nad acid mucins, glycogens and glycoproteins. (biognost.com)
  • Alcian Blue dye stains acid mucins that turn insoluble and resistant to remaining reagents during P.A.S. staining procedure. (biognost.com)
  • Alcian Blue is used to prove glycosaminoglycan in mucins, for staining amyloids, cysteines and for polychromatic staining of mastocytes according to Blue-Safranin. (biognost.com)
  • Peripheral lung sections from smokers with COPD (n = 9) and age-matched controls including smokers (n = 11) and lifelong non-smokers with normal lung function (n = 6) were stained with alcian blue, periodic acid-Schiff (PAS) and by immunohistochemistry of mucins (MUC): MUC2, MUC4, MUC5AC, MUC5B and MUC6. (nih.gov)
  • Neutral and acidic mucins were identified by Periodic-acid Schiff and Alcian-Blue techniques, respectively. (scielo.br)
  • Combining P.A.S. staining and Alcian Blue dye improves the identification of acid mucosubstances (glycosaminoglycan). (biognost.com)
  • The amount of Alcian blue/periodic acid-Schiff-stained mucosubstances was determined by morphometry. (cdc.gov)
  • Results: The established protocol successfully generated a large quantity of viable chondrocytes, characterized by alcian blue and toluidine blue staining, and demonstrated versatility in assessing the anti-inflammatory effects of various bioactive compounds. (lu.se)
  • Seven-reagent Alcian Blue - Periodic Acid-Schiff kit for staining acid mucopolysaccharides according to Mowry. (biognost.com)
  • Paraffin sections were stained with either an aqueous mixture of alcian blue 8GX, safaranin 0 and Bismark brown Y, or with the periodic acid - Schiff (PAS) reaction. (usda.gov)
  • 1 Alcian blue stained the myxoid matrix strongly. (vin.com)
  • Alcian blue-positive myxoid stroma at the interface between the fibrovascular proliferation and the epithelial-stromal dome was present in 65 percent of samples. (healthday.com)
  • There was a significant association between the presence of fibrovascular proliferation and Alcian blue-staining myxoid stroma. (healthday.com)
  • An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. (sigmaaldrich.com)
  • The samples, fixed in 10% buffered neutral formalin, were paraffin embedded and the sections were stained for H/E and Alcian blue/PAS at pH 2.5. (ispub.com)
  • The process has been refined through the utilization of optical projection tomography, which effectively 3D-images Alcian Blue stained cartilage in cleared specimens. (news-medical.net)
  • Dingerkus, G. & Uhler, L.D. (1977) Enzyme clearing of alcian blue stained whole small vertebrates for demostration of cartilage. (mapress.com)
  • Nuclei are stained with Hematoxylin ML (Mayer-Lillie) that does not interfere with Alcian Blue dye. (biognost.com)
  • You should always get a seal from the Biological Stains Commission on each bottle of Alcian Blue powder. (utsouthwestern.edu)
  • In the past few years, Dudley Chemical, Aldrich, Sigma, EM Science and some Chinese companies were able to produce synthetic Alcian Blue, although the dye content varies with each companies submission. (utsouthwestern.edu)
  • When I started my R&D on Alcian Blue for Biocare, Anatech had the highest dye content from the few companies that sold it. (utsouthwestern.edu)
  • Mucus can be stained with the Alcian blue method, which can facilitate the identification of this lesion. (nih.gov)
  • It was my understanding and I could be wrong, that for awhile, Anatech was the only company who produced Alcian Blue to pass the Biological Stains Commission evaluation process. (utsouthwestern.edu)
  • Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices. (sigmaaldrich.com)
  • Although the compound arsine blue itself is unstable (see Stability below), the resulting stain is stable and lightfast. (adpostman.com)
  • Notes on Technic: Note from the Biological Stain Commission a Newly Certified Dye-Alcian Blue 8GX. (adpostman.com)
  • Histological examination of myxomas shows characteristic hypocellular areas of sparse strands of cells suspended in a rich myxoid matrix, strongly positive on Alcian blue stain. (upmc.edu)
  • 36. Mucin secretion by gastric carcinoma cells: PAS alcian blue stain study. (nih.gov)
  • The ground substance is amorphous, light pink, and sometimes slightly granular in appearance and typically consists of acid mucopolysaccharides that stain positive with Alcian blue at pH 2.5 (humans, equids). (nih.gov)
  • Amyloid deposits stain pink with hematoxylin and eosin, contain carbohydrate constituents that stain with periodic acid-Schiff dye or with Alcian blue, but most characteristically have apple-green birefringence under polarized light microscopy after Congo red staining. (msdmanuals.com)
  • life history of iron and alcian blue stain ph. (familytreecounseling.com)
  • 1 Alcian blue stained the myxoid matrix strongly. (vin.com)
  • In the aqueous solution, Alcan blue is discolored even when the molar concentration is one hundred percent of the molar concentration of toluidine blue, which is the primary color. (adpostman.com)
  • Cartilage has reduced staining with cationic dyes, such as toluidine blue or Alcian blue, probably because of a deficiency in sulfated proteoglycans. (medscape.com)
  • To ensure Alcian blue positivity, specific tissue preparation and processing are necessary. (nih.gov)
  • Overlying small intestinal mucosa showed goblet cells with Alcian blue positivity (Figure 2c). (austinpublishinggroup.com)
  • These glands below the muscularis were PAS positive and Alcian blue negative, which confirmed the neutral mucin in these glands suggesting a foveolar epithelium of gastric mucosa (Figures 2c,2d). (austinpublishinggroup.com)
  • The vacuolated cells contain PAS- and/or Alcian blue-positive material (mucin). (nih.gov)
  • The myxoid background stains strongly with Alcian blue. (upmc.edu)
  • 28. Evaluation of Better Staining Method among Hematoxylin and Eosin, Giemsa and Periodic Acid Schiff-Alcian Blue for the Detection of Helicobacter pylori in Gastric Biopsies. (nih.gov)
  • An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. (sigmaaldrich.com)
  • differentiation of LB cells was assessed by Alcian blue staining of extracellular proteoglycans. (nih.gov)
  • The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. (sigmaaldrich.com)
  • Alsin blue can be used for quantification of acid glycans in differential spectrophotometric quantification in solution, and for staining glycoproteins in polyacrylamide gels or Western blots. (adpostman.com)
  • Then, the ECM was assessed by two histochemical methods: Picrosirius Red (PR) staining for 30 min with Sirius red (0.1% of Sirius red in saturated aqueous picric acid), for collagen bundle staining, and Alcian Blue (AB) staining for glycosaminoglycans detection. (ugr.es)
  • The glandular cells were positive to PAS and negative to Alcian blue and showed strong positive activity for acid phosphatase. (hilarispublisher.com)
  • Alcian Blue is any member of the family of multivalent basic dyes, of which Alcian Blue 8G has historically been the most common and reliable member. (adpostman.com)
  • Histological samples of branches were prepared by cryo-sectioning, stained with carbohydrate binding Alcian Blue and lignin binding Safranin dyes to reveal patterns of tension wood development. (helsinki.fi)
  • The aqueous solution was bright green blue. (adpostman.com)
  • In the aqueous solution, a large number of Arsin blue molecules accumulate into very large micelles that are too large to even be dialyzed. (adpostman.com)
  • Hito Alcian Blue pH-1.0 OptimStain™ Kit is formulated for the staining of strongly sulfated mucosubstances. (hitobiotec.com)
  • Historically, Arsin Blue has been a popular staining method in histology, especially in light microscopes with paraffin-embedded sections and semi-thin resin sections. (adpostman.com)
  • After treatment, rats were euthanized, and tail tissues were chemically fixed for histological analysis of nerves (PGP9.5, neuropeptide Y, calcitonin gene related protein) and mast cells (avidin, Alcian Blue/Safranin O).2 Single sections were doublestained for nerve fibers and mast cells to permit assessment of the degree of physical overlap. (cdc.gov)
  • This study showed that plastination technique was an appropriate method in comparison with glycerin preservation, which conserved skeletal tissue of fetus and young rats colored by Alizarin red- Alcian blue double staining. (ac.ir)
  • This study aimed to compare the new plastination technique with the conventional preservative method in glycerin for fetus skeleton tissues and young rats dyed by Alizarin red- Alcian blue double staining. (ac.ir)
  • We also present an improved method of calibrating the Alcian Blue solution against Gum Xanthan for TEP measurements. (nerc.ac.uk)
  • Hito Alcian Blue pH-1.0 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a simple solution for your research. (hitobiotec.com)
  • The catalytic experiment with alcian blue 8Gx dye under visible light showed higher degradation than in dark, which illustrated the photocatalytic nature of the material. (ualr.edu)
  • Enzyme clearing of alcian blue stained whole small vertebrates' dor demonstration of cartilage. (ac.ir)
  • Ranjbar R, Vatanchian M, Mohammadian B, Mayahi M. Chronological study of chicken embryo wing and leg skeleton development by alizarin red- alcian blue double staining technique. (ac.ir)
  • Masson's and Alcian blue-PAS staining revealed evidence of fibrosis in the Galnt11 null kidneys. (nih.gov)
  • Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. (sigmaaldrich.com)