• The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. (nih.gov)
  • A humanized yeast model reveals dominant-negative properties of neuropathy-associated alanyl-tRNA synthetase mutations. (nih.gov)
  • Alanyl-tRNA synthetase 1 (AARS1) gene mutation in a family with intermediate Charcot-Marie-Tooth neuropathy. (nih.gov)
  • 4560 valyl-tRNA synthetase CP001857 CDS Arcpr_0004 4868. (go.jp)
  • 55056) tyrosyl-tRNA synthetase CP001857 CDS Arcpr_0065 complement(55112. (go.jp)
  • This domain is found at the amino terminus of Arginyl tRNA synthetase, also called additional domain 1 (Add-1). (embl-heidelberg.de)
  • Crystal structure of arginyl-tRNA synthetase from Klebsiella pneumoniae subsp. (embl-heidelberg.de)
  • For this purpose, segments of the Hsp60 (groEL), Hsp70 (dnaK), CTP synthase and alanyl-tRNA synthetase genes, which are known to contain signature sequences that are useful for phylogenetic deterministic purposes, were cloned. (mcmaster.ca)
  • Using degenerate oligonucleotide primers for highly conserved regions in these proteins, 1.4 kb, 0.75 kb, 401 bp and 171 bp fragments of the Hsp70, Hsp60, CTP synthase and alanyl-tRNA synthetase genes respectively were amplified by PCR, and these fragments were cloned and sequenced. (mcmaster.ca)
  • A particularly useful signature consisting of a 4 aa insert is found in Ala-tRNA synthetase. (mcmaster.ca)
  • The presence of this insert in F. succinogenes Ala-tRNA synthetase provides evidence that this species is related to these groups. (mcmaster.ca)
  • Alanyl-tRNA synthetase (PL-12) is specific for the amino acid alanine. (bbisolutions.com)
  • Genetic code expansion (GCE) technologies commonly use the pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs from Methanosarcina mazei (Mm) and Methanosarcina barkeri (Mb) for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. (oregonstate.edu)
  • This enzyme participates in alanine and aspartate metabolism and aminoacyl-trna biosynthesis. (wikipedia.org)
  • Significant (after false discovery rate correction) KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways included aminoacyl-tRNA biosynthesis, arginine biosynthesis, and metabolism of multiple amino acids. (nature.com)
  • Holley's research on RNA focused first on isolating transfer RNA (tRNA), and later on determining the sequence and structure of alanine tRNA, the molecule that incorporates the amino acid alanine into proteins. (myrank.co.in)
  • Silks are composed principally of proteins with a predominance of alanine, serine and glycine and silk proteins are able to undergo irreversible transformations from soluble protein to insoluble fibres. (bioone.org)
  • The threonine-specific tRNA releases its threonine and leaves and the new protein floats away. (howstuffworks.com)
  • Rqc2p stabilizes Ltn1p on the 60S and recruits charged tRNAs to the 60S to catalyze elongation of the nascent protein with carboxy-terminal alanine and threonine extensions (CAT tails). (escholarship.org)
  • The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. (embl-heidelberg.de)
  • However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. (embl-heidelberg.de)
  • In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. (embl-heidelberg.de)
  • For mRNA translation at the ribosomes, amino acids are covalently attached ("loaded") onto their respective tRNAs by specific amino-acyl-tRNA synthetases. (bbisolutions.com)
  • 42520) hypothetical protein CP001857 tRNA Arcpr_R0002 complement(42639. (go.jp)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing Arg_tRNA_synt_N domain in the selected taxonomic class. (embl-heidelberg.de)
  • Human cells infected with SARS-CoV-2 exhibit a decrease in TRMT1 protein levels and TRMT1-catalyzed tRNA modifications, consistent with TRMT1 cleavage and inactivation by Nsp5. (elifesciences.org)
  • Alanine is highly concentrated in meat products and other high-protein foods like wheat germ and cottage cheese. (cannabisdatabase.ca)
  • He shared the Nobel Prize in Physiology or Medicine in 1968 (with Har Gobind Khorana and Marshall Warren Nirenberg) for describing the structure of alanine transfer RNA, linking DNA and protein synthesis. (myrank.co.in)
  • Upon ribosome stalling and splitting, the RQC assembles on the 60S species containing unreleased peptidyl-tRNA (60S:peptidyl-tRNA). (escholarship.org)
  • 5. A tRNA molecule that is supposed to carry cysteine (tRNAcys) is mischarged so that it actually carries alanine (ala-tRNAcys). (entri.app)
  • There are 26085 Arg_tRNA_synt_N domains in 26083 proteins in SMART's nrdb database. (embl-heidelberg.de)
  • Taxonomic distribution of proteins containing Arg_tRNA_synt_N domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with Arg_tRNA_synt_N domain is also avaliable . (embl-heidelberg.de)
  • Purification of individual SARS-CoV-2 proteins from human cells have identified a potential interaction between a catalytic-inactive version of Nsp5 with human tRNA methyltransferase 1 (TRMT1) ( Gordon et al , 2020b ). (elifesciences.org)
  • Belongs to the class of organic compounds known as alanine and derivatives. (cannabisdatabase.ca)
  • Alanine and derivatives are compounds containing alanine or a derivative thereof resulting from reaction of alanine at the amino group or the carboxy group, or from the replacement of any hydrogen of glycine by a heteroatom. (cannabisdatabase.ca)
  • Normal alanine metabolism, like that of other amino acids, is highly dependent upon enzymes that contain vitamin B6. (cannabisdatabase.ca)
  • Alanine is an important participant as well as a regulator of glucose metabolism. (cannabisdatabase.ca)
  • L-Alanine has been found to be associated with glucagon deficiency, which is an inborn error of metabolism. (cannabisdatabase.ca)
  • This tRNA is unnecessary for growth, but is required for some aspects of secondary metabolism and morphological development. (biomedcentral.com)
  • Functionally, QXHC candidate targets were significantly associated with several biological pathways, such as VEGF and Chemokine signaling pathways, Alanine/aspartate/glutamate metabolism, Long-term depression and T/B cell receptor signaling pathway. (biomedcentral.com)
  • Here, we found that this strategy produced low-efficiency Ma PylRSs for encoding three structurally diverse ncAAs: acridonyl-alanine (Acd), 3-nitro-tyrosine, and m-methyl-tetrazinyl-phenylalanine (Tet3.0-Me). (oregonstate.edu)
  • Here we identify Vms1p as a tRNA hydrolase that releases stalled polypeptides engaged by the RQC. (escholarship.org)
  • The systematic name of this enzyme class is L-alanine:tRNAAla ligase (AMP-forming). (wikipedia.org)
  • Here, we show that Nsp5 binds and cleaves human tRNA methyltransferase 1 (TRMT1), a host enzyme required for a prevalent post-transcriptional modification in tRNAs. (elifesciences.org)
  • This manuscript provides important insights into the degradation of a host tRNA modification enzyme TRMT1 by SARS-CoV-2 protease nsp5. (elifesciences.org)
  • Holley's team of researchers determined the tRNA's structure by using two ribonucleases to split the tRNA molecule into pieces. (myrank.co.in)
  • B ) Relative amounts of amino acids liberated from tRNA isolated from the pancreatic islets of 24-week-old NCD- or HFD-fed WT mice were determined by metabolome analysis ( n =6, 1 sample contains islets from 2 mice). (jci.org)
  • They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. (nih.gov)
  • tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. (nih.gov)
  • The TRMT1 cleavage fragments exhibit altered RNA binding activity and are unable to rescue tRNA modification in TRMT1-deficient human cells. (elifesciences.org)
  • Alanine levels parallel blood sugar levels in both diabetes and hypoglycemia, and alanine reduces both severe hypoglycemia and the ketosis of diabetes. (cannabisdatabase.ca)
  • These findings provide evidence that Nsp5-dependent cleavage of TRMT1 and perturbation of tRNA modification patterns contribute to the cellular pathogenesis of SARS-CoV-2 infection. (elifesciences.org)
  • Class II tRNA synthases evolved early in evolution and are highly conserved. (nih.gov)
  • It is about 140 residues long and it has been suggested that this domain will be involved in tRNA recognition. (embl-heidelberg.de)
  • Recently a homologous PylRS/tRNAPyl pair from CandidatusMethanomethylophilus alvusMx1201 (Ma) was developed that, lacking the N-terminal tRNA-recognition domain of most PylRSs, overcomes insolubility, instability, and proteolysis issues seen with Mb/Mm PylRSs. (oregonstate.edu)
  • Amino acid levels are reduced and uncharged tRNA levels are increased in the islets of mice fed a HFD. (jci.org)