Alanine scanning library. Medical search. Frequent questions

In molecular biology, alanine scanning is a site-directed mutagenesis technique used to determine the contribution of a specific residue to the stability or function of a given protein. (wikipedia.org)
This is usually accomplished by site-directed mutagenesis or randomly by creating a PCR library. (wikipedia.org)
Then shotgun scanning method which combines the concepts of alanine scanning mutagenesis and binomial mutagenesis with phage display technology was used. (wikipedia.org)
Alanine scanning mutagenesis of the alpha-helix 115-123 of phage T4 lysozyme: effects on structure, stability and the binding of solvent. (expasy.org)
Using 2C6 and variants, alanine scanning mutagenesis identified three amino acids (aa 592, 595, and 596) in the overlapping region of the previously identified peptide. (nih.gov)
Epitope mapping using shotgun mutagenesis alanine scanning across the 509 amino acids of GLUT4 identified the binding epitopes for mAbs specific for the states of GLUT4 and allowed the comprehensive identification of the residues that functionally control the GLUT4 inward-open and outward-open states. (pubchase.com)

One good example of alanine scanning is the examination of the role of charged residues on the surface of proteins. (wikipedia.org)
In a systematic study on the roles of conserved charged residues on the surface of epithelial sodium channel (ENaC), alanine scanning was used to reveal the importance of charged residues for the process of transport of the proteins to the cell surface. (wikipedia.org)
Another critical application of alanine scanning is to determine the influence of individual residues on structure and activity in the prototypic cyclotide kalata B1. (wikipedia.org)
On the structure of cyclotides kalata B1, all 23 non-cysteine residues were successively substituted with alanine. (wikipedia.org)
In this study, several Cry4Aa mutants were made by replacing the residues of potential receptor binding site, loops 1, 2, and 3 in domain II with alanine. (wikipedia.org)
We performed an alanine scan of the non-glycine residues up through residue 11 of Dyn B amide to explore the role of these side chains in the activity of Dyn B. The analogs were synthesized by fluorenylmethyloxycarbonyl (Fmoc)-based solid phase peptide synthesis and evaluated for their opioid receptor affinities and opioid potency and efficacy at KOR. (ku.edu)
From affirming new leads with a scrambled library to identifying essential residues, a PepSet peptide library can offer more than the sum of its peptides. (nridigital.com)
Although smaller libraries are much easier and less expensive to synthesize, the likelihood of researchers finding the precise interface residues in complex projects is often limited. (proteogenix.science)
In contrast, when offset numbers are low and peptides short, the resulting library will have more peptides, making it easier to identify the interface residues but also resulting in more time-consuming experiments. (proteogenix.science)
Alanine scan of ARTS 266-274 showed the importance of several residues for Bir3 binding, with His268 and Cys273 contributing the most. (huji.ac.il)
Alanine scanning of conserved residues located between the glutaminase and synthase active sites revealed a network of mainly charged residues that lead to activity-inducing conformational changes at the glutaminase subunit. (uni-regensburg.de)

Find the cause of abnormal blood test results from aspartate aminotransferase (AST) and alanine aminotransferase (ALT) tests. (healthplan.org)
Liver function tests, especially alanine aminotransferase (ALT) and aspartate aminotransferase (AST), should be monitored. (medscape.com)

As one of the prominent applications of combinatorial chemistry, parallel synthesis techniques allow hundreds of peptides to be synthesised simultaneously into PepSet peptide libraries. (nridigital.com)
Suitable for applications such as epitope mapping and screening, a variety of PepSet preset combinatorial library and array styles are available. (nridigital.com)
These include overlapping peptides, alanine scanning, combinatorial positional scanning, truncated peptide libraries, and scrambled peptide libraries, as well as combinatorial libraries comprised of more than one different type. (nridigital.com)
Binary combinatorial scanning reveals potent poly-alanine-substituted inhibitors of protein-protein interactions. (u-tokyo.ac.jp)

Alanine is used because of its non-bulky, chemically inert, methyl functional group that nevertheless mimics the secondary structure preferences that many of the other amino acids possess. (wikipedia.org)
The alanine scanning method takes advantage of the fact that most canonical amino acids can be exchanged with Ala by point mutations, while the secondary structure of mutated protein remains intact, as Ala mimics the secondary structure preferences of the majority of the encoded or canonical amino acids. (wikipedia.org)

This vigilance demand for and physician delivery of computed tomography (CT) scans of should involve rapid investigation antirabies prophylaxis. (cdc.gov)
Advances in radiologic imaging techniques, such as computed tomography (CT) scanning and magnetic resonance imaging (MRI), have improved the ability to predict the success for complete removal of the mass. (medscape.com)
Computed tomography (CT) scanning has a limited sensitivity (63%) for the assessment of hepatic iron overload. (medscape.com)

For this tutorial we have chosen to mutate residue 21 (Isoleucine, I21) to alanine because this is a residue that is found at the interface between the receptor and ligand and should have a noticeable effect on the binding energy. (ambermd.org)

M imotopes provides custom peptide synthesis, catalogue peptides, PepSet™ peptide libraries, reagents, antibodies, and an array of other immunology services for the research market. (nridigital.com)

With years of experience, we can offer high-quality phage display library construction and custom phage display library screening services to meet your various demands precisely. (cusabio.com)
In short, a phage library expressing a wide diversity of peptides or proteins is used to select those binding the desired targets. (cusabio.com)
Based on different phages, vectors or libraries, the phage display systems could be divided into various types, details as below. (cusabio.com)
CUSABIO could provide phage display technologies to customers that cover the broad scope of the technologies including key areas of library construction and screening as well as antibody modification and expression. (cusabio.com)
The phage display library technology refers to the clone and expression for variable region genes of all antibodies from a certain animal in a plasmid or phage, then screen clones carrying specific antibody genes via different antigens, thereby, to obtain corresponding specific antibodies or peptides. (cusabio.com)
To package the antibody library via infecting M13K07 phage. (cusabio.com)
Infect phage via TG1, then detect antibody library titer via colony counting. (cusabio.com)
Compared with the traditional hybridoma method, antibody phage display library has distinct advantages on selecting novel monoclonal antibodies and even the fully humanized antibody. (cusabio.com)
Furthermore, the antibodies from phage library have following advantages. (cusabio.com)
By a combination of approaches including (i) screening of phage-displayed random peptide libraries, (ii) mapping of the binding regions on three physiological interactors of Eps8, (iii) alanine scanning of binding peptides and (iv) in vitro cross-linking, we demonstrate that a proline-X-X-aspartate-tyrosine (PXXDY) consensus is indispensable for binding to the SH3 domain of Eps8. (hal.science)

Peptide libraries are collections of synthetic peptides, invaluable for studying protein-protein interactions. (proteogenix.science)

Alanine Scanning was used to determine simultaneously the functional contributions of 19 side chains buried at the interface between human growth hormone and the extracellular domain of its receptor. (wikipedia.org)
Alanine scanning of a putative receptor binding surface of insulin-like growth factor-I". J. Biol. (wikipedia.org)
Through screening and profiling applications, libraries help researchers determine relevant antigen-antibody recognition sites, unravel enzyme-substrate specificity, or determine the sites responsible for receptor binding - particularly useful when studying pathogenic viruses. (proteogenix.science)

The present invention is based on successful expression cloning of a transcription factor that binds to the GTII element of the GLUT2 and insulin genes from a differentiated insulin-secreting cDNA library. (justia.com)

This complex is advantageous for the immobilisation of a peptide library, particularly for use in multiple assays. (nridigital.com)
Our customized peptide libraries with flexible layout and specifications are optimized for deep T-cell epitope discovery using ELISpot and intra-cellular cytokine staining assays. (jpt.com)

When developing a novel biologic for a target protein, we perform structure-based virtual screening of our structural libraries in order to identify potential hits with preliminary complementarity in shape and chemistry. (silicogenesis.com)
3) Perform an alanine scanning calculation on the binding free energy of Ras-Raf. (ambermd.org)

The captured antibodies in one or more of the containers are exposed to an antigen display library comprising a plurality of display particles for a time sufficient to allow for interaction of one or more of the captured antibodies with the one of more of the display particles. (justia.com)

Also, one topic that comes up repeatedly (for example here and here ) but has never been actually polled is how many fragments make a good library - we've added a question about that too. (blogspot.com)
3000 fragments from the Chembridge library were screened. (blogspot.com)

In addition, alanine scanning is also used to determine which functional motif of Cry4Aa has the mosquitocidal activity. (wikipedia.org)

Methods for identifying such antibodies have been previously described and include hybridoma approaches, display library approaches, B-cell immortalization, B-cell cloning, and single cell screening methods. (justia.com)

Reduce the costs of your overlapping peptide library screening experiments without sacrificing data quality. (proteogenix.science)

describe the method of combining simulated annealing with fragment libraries to generate native-like structures. (rosettacommons.org)
With ProteoGenix's cost-effective method of peptide library synthesis , you can now employ high-quality peptides for a fraction of the cost. (proteogenix.science)

Note the current version of the code will support mutations to alanine only. (ambermd.org)

We assist with target selection, sequence analysis/bioinformatics and peptide library design. (jpt.com)
We observe that tissue type and library size selection have considerable impact on mitogenome sequencing and assembly. (biomedcentral.com)

The ratio between length and offset dictates the size of the overlapping peptide library and thus determines the quality of the data derived from the experiment. (proteogenix.science)

Each amino acid in the side chains was substituted by alanine. (wikipedia.org)
The side chain of alanine is -CH 3 . (ambermd.org)
Since the side chain of isoleucine has more atoms than the side chain of alanine, we know that we must remove atoms and their corresponding information (name, number, coordinates, etc.) from the pdb files. (ambermd.org)

This is predicted by the Alanine-World model. (wikipedia.org)

Significant factors contributing to recurrence include atypical and malignant histologic types and heterogeneous tumor contrast enhancement on CT scans. (medscape.com)

The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. (wikigenes.org)

Alternatively, we design libraries of de novo protein scaffolds using the structure of the target protein. (silicogenesis.com)

Article added to library! (pubchase.com)

Check a liver mass found on an X-ray, ultrasound, MRI , or CT scan. (healthplan.org)

8. At least 1 measurable target intrahepatic lesion suitable for repeat assessments according to the following mRECIST criteria: Liver lesions that show typical features of HCC on IV contrast-enhanced CT or MRI scans, ie, hypervascularity in the arterial phase with washout in the portal or the late venous phase. (who.int)

CUSABIO is committed to offering quality services of antibody library construction as well as peptide library construction to customers. (cusabio.com)
CUSABIO is mainly engaged in offering quality and personalized services of antibody library screening as well as peptide library screening to meet customers' various demands. (cusabio.com)

CUSABIO can construct antibody libraries as well as screen desired monoclonal antibodies with high specificity and affinity from a wide range of species, such as human, alpaca, mouse, rabbit, chicken, dog, bovine, and so on. (cusabio.com)

Furthermore, computational methods to estimate thermodynamic parameters based on theoretical alanine substitutions have been developed. (wikipedia.org)

Mimotopes' time-saving strategies economically produce entire peptide libraries with the same customisation options as a single custom peptide. (nridigital.com)

The Health Library is a collection of health and wellness resources created for learning and accessibility. (healthplan.org)

Why choose ProteoGenix for overlapping peptide library synthesis? (proteogenix.science)
We do not need to add the beta-hydrogen (HB) atoms because tleap will add those in the proper locations based on the particular library files you choose for your system. (ambermd.org)

Peptide library scanning showed consistent binding in this region but to only a single peptide. (nih.gov)

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