Airborne vitro and cell. Medical search. Frequent questions

The chambers are room size (total volume around 64 m3) with inner surfaces of stainless steel to enable precise control of environmental parameters and airborne particles. (lu.se)
Airborne particles in the size range 60-1000 nm are focused by means of an aerodynamic lens and subsequently vaporized. (lu.se)
To compare these novel methods with the conventional cell-exposure techniques relying on submerged cell systems, where aerosol particles are collected in water with high-volume samplers. (admin.ch)
Until now this platform is mainly used for laboratory studies (generation and exposure of pollutants, e.g. nano and ultrafine particles, and effect measurements in human lung cell models), but current developments are pointing towards the use of the ALI systems in real-life settings. (nanotechia.org)
Researchers have found that dispersants utilized to clean up oil spills can lead to lung damage when airborne particles of these dispersants combine with crude oil and are inhaled. (mit.edu)
The purpose of the international research project NANOaers (= Fate of aerosolised nanoparticles: The influence of surface active substances on lung deposition and respiratory effects ) is to establish where airborne, respirable particles remain after they have been inhaled. (bund.de)
That report reveals UV lamps set between 207 and 222 nanometers can kill 90 percent of airborne virus particles in just eight minutes. (studyfinds.org)
Airborne precautions are standard to contain measles, tuberculosis, chickenpox, and herpes zoster, in which very small particles spread in the air," he said. (the-hospitalist.org)
Airborne particles have been associated with a range of adverse cardiopulmonary outcomes, which has driven its monitoring at stationary, central sites throughout the world. (cdc.gov)
Such metrics provide opportunities to develop more precise associations between airborne particles and health and may provide opportunities for more effective regulations. (cdc.gov)
Airborne spores and other fungi particles are ubiquitous in nonpolar landscapes, especially among field crops, and often form the bulk of suspended biogenic debris. (medscape.com)

Toxicol In Vitro 2006, 20, 1522-1531. (aaem.pl)
Toxicol in Vitro 45, 417-425. (altex.org)

Exposure of human populations to smoke emitted by these fires increases, thereby contributing to airborne pollution through the emission of gas and particulate matter (PM). The adverse health outcomes associated with wildland fire exposure represent an important burden on the economies and health systems of societies. (frontiersin.org)
These effects depend not only on physical, but also on chemical properties of airborne particulate matter (PM) though to date it has proven difficult to disentangle the relative contribution of PM constituents to the reported population-level health effects. (admin.ch)
Particulate matter from both heavy fuel oil and diesel fuel shipping emissions show strong biological effects on human lung cells at realistic and comparable in vitro exposure conditions. (zaum-online.de)
The coarse and fine fractions of airborne particulate matter caused induction of the heat shock protein 70 in vitro, whereas the ultrafine fraction did not [ 17 ]. (biomedcentral.com)
For example, we found that cigarette smoke extract, fossil fuel-derived particulate matter and welding fumes, by means of the induction of oxidative stress, upregulate PAFR-dependent pneumococcal adhesion to lower airway cells in vitro [ 8 - 10 ]. (ersjournals.com)

However, data from in vitro and in vivo studies on the interaction between these pathogens remain scarce. (biomedcentral.com)

This will be delivered through the development of a new method for studying in vitro cytotoxicity based on the use of "tailored" synthetic ambient aerosols combined with high-resolution optical imaging and state-of-the-art cell analysis methods. (admin.ch)
Uncover its applications in cell viability testing, cytotoxicity, cell proliferation, and high throughput screening services. (acmeresearchlabs.in)
An in vitro cytotoxicity study is an assay to determine the effect of a substance on cells in a culture. (acmeresearchlabs.in)
Cytotoxicity Test by MTT Assay- MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test is used to measure the cellular metabolic activity in mammalian cells as an indicator of cell viability, proliferation and cytotoxicity. (acmeresearchlabs.in)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)
In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). (bvsalud.org)
The epoxy paint studied showed no cytotoxicity in vitro. (bvsalud.org)

The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
Treatment with the anti-AREG antibody prevented resolution of DE exposure-induced airway influx of total cells, neutrophils, and macrophages and increased levels of TNF-a, IL-6, and CXCL1. (cdc.gov)
To apply novel methods for cell exposure at the air-liquid interface (ALI) in order to mimic and quantify the effects of the in vivo aerosol inhalation routes. (admin.ch)
VITO is active in the field of nanosafety through determination of nanoparticle exposure/release potential, airborne emission characterization, exposure prevention and training in support of safe nanomaterial development and use along the life cycle. (nanotechia.org)
In vitro methods provide a key opportunity to consider human-relevant exposure scenarios for hazard identification of inhaled toxicants. (altex.org)
Compared to in vivo tests, in vitro methods have the advantage of assessing effects of inhaled toxicants expressed by differences in dosimetry, e.g. variations in concentration (exposure intensity), exposure duration and exposure frequency, in an easier way. (altex.org)
9. Airborne olive pollen counts are not representative of exposure to the major olive allergen Ole e 1. (zaum-online.de)
PVP-I 7% gargle/mouthwash showed rapid bactericidal activity and virucidal efficacy in vitro at a concentration of 0.23% PVP-I and may provide a protective oropharyngeal hygiene measure for individuals at high risk of exposure to oral and respiratory pathogens. (springer.com)
In the current study, we examined temporal aspects of the exposure-response relationship between airborne endotoxin exposure, longitudinal change in FEV 1 , and respiratory symptoms in a cohort of Chinese cotton textile workers. (nih.gov)
T cells become sensitized to beryllium and then proliferate on re-exposure. (msdmanuals.com)

The Aerosol Laboratory is equipped with state-of-the-art tools for physical, chemical, and biological characterization of airborne nanoparticles and is open for collaborations in nanoscience and nanosafety. (lu.se)
Attana was founded in 2002 with the vision of in vitro characterization of molecular interactions mimicking in vivo conditions. (cision.com)

fungi, which are present in soil in the southwestern United States, can become airborne when the soil is disrupted, and humans who inhale the spores can become infected. (cdc.gov)
and is acquired through inhalation of airborne spores. (cdc.gov)
As the weather dries, arthroconidia break off and become airborne spores when the soil is disrupted ( 7 ). (cdc.gov)
Abdel-Hafez SII: Survey of airborne fungus spores at Taif, Saudi Arabia. (aaem.pl)

Al-Suwaine AS, Bahkali AH, Hasnain SM: Seasonal incidences of airborne fungal allergens in Riyadh, Saudi Arabia. (aaem.pl)
Fungi are ubiquitous airborne allergens and are important causes of human diseases, especially in the upper and lower respiratory tracts. (medscape.com)

14 The kinetic limulus amoebocyte lysate (LAL) method, an in vitro biological method, has been widely used for endotoxin measurements since the 1980s. (bmj.com)
4. Oteros J, Bartusel E, Núñez-Hernández A, Moreno-Gómez D, Behrendt H, Schmidt-Weber C, Traidl-Hoffmann C, Buters J . Artemisia pollen is the main vector for airborne endotoxin. (zaum-online.de)

In vitro toxicity studies play an important role in the development of new drugs and therapies. (acmeresearchlabs.in)
Tetrazolium reduction and resazurin assays are the mainstay of routine in vitro toxicity batteries. (cdc.gov)

The findings are published in mBio in an article titled, " A new tractable method for generating human alveolar macrophage-like cells in vitro to study lung inflammatory processes and diseases . (genengnews.com)
Alveolar macrophages (AMs) are unique lung resident cells that contact airborne pathogens and environmental particulates," wrote the researchers. (genengnews.com)
We developed cell culture conditions that mimic the lung alveolar environment in humans using lung lipids, that is, Infasurf (calfactant, natural bovine surfactant) and lung-associated cytokines (granulocyte macrophage colony-stimulating factor, transforming growth factor-β, and interleukin 10) that facilitate the conversion of blood-obtained monocytes to an AM-like (AML) phenotype and function in tissue culture. (genengnews.com)
The generated cells are 94% genetically similar to human alveolar macrophages collected from lung washes. (genengnews.com)
I am excited to see the full potential of the alveolar macrophage-like cells and if they can be integrated into next-generation lung organoids," Schlesinger added. (genengnews.com)
The compounds suppressed SARS-CoV-2 replication in cell culture cells and a patient-near replication system using human precision-cut lung slices by two orders of magnitude. (uni-wuerzburg.de)
Here, we present a novel model for generating AML cells based on differentiating blood monocytes in a defined lung component cocktail. (bvsalud.org)
In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)
Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)
An in vitro lung system to assess the proinflammatory hazard of carbon nanotube aerosols. (altex.org)
Lung epithelial cell death induced by oil-dispersant mixtures. (mit.edu)

the cells readily take up the pathogens. (genengnews.com)
We investigated the in vitro bactericidal and virucidal efficacy of povidone-iodine (PVP-I) 7% gargle/mouthwash at defined dilution against oral and respiratory tract pathogens. (springer.com)
Respiratory pathogens such as influenza are also transmitted via airborne dispersion of small particle aerosols (≤ 5 µm) when an infected individual breathes, coughs or sneezes [ 11 ], while respiratory syncytial viruses, SARS-CoV and MERS-CoV can be spread by large droplets propelled through the air and inoculated into the eyes, nose and mouth at close range [ 12 ]. (springer.com)
This study aimed to determine the risk factors and in vitro antibiotic susceptibility patterns of bacterial pathogens associated with neonatal sepsis in Federal Medical Centre (FMC) and Turai Umaru Yar'adua Maternal and Children Hospital (TUYMCH), Katsina, Nigeria. (bvsalud.org)

To assess how the composition of the collected aerosols and their ageing impacts on their acellular and cellular oxidative characteristics, both in simple chemical models simulating human respiratory tract lining fluids (in health and disease) and in representative cell lines maintained under near physiologic conditions. (admin.ch)
Although it is widely believed that transmission of B. pertussis occurs via aerosolized respiratory droplets, no controlled study has ever documented airborne transmission of pertussis. (thisisbeep.com)
Unlike the wild type H5N1, this mutant virus was transmitted by direct contact in the ferret model although not by airborne respiratory droplets. (nih.gov)
Similar to HAM, AML cells are particularly susceptible to both Mycobacterium tuberculosis and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. (bvsalud.org)
As the cell models used are models for the respiratory epithelium, our focus is on the local effects in the airways. (altex.org)
Tuberculosis is a communicable disease caused by infection with M. tuberculosis complex organisms, which typically spreads to new hosts via airborne droplet nuclei from patients with respiratory tuberculosis disease. (cdc.gov)
Since invasive pneumococcal strains adhere to, and translocate across, respiratory tract epithelial cells, adhesion is a prerequisite for establishing pneumococcal disease [ 4 ]. (ersjournals.com)
Covid-19 is a virus that is transmitted primarily as an airborne pathogen through the upper respiratory tract. (medstartr.com)

In Vitro Assay Services in Dehradun: In the burgeoning field of biotechnology and pharmaceuticals, in vitro assays have become instrumental in our understanding of biological processes and drug development. (acmeresearchlabs.in)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
Since then, Attana has developed proprietary label free biosensors for biochemical, crude, sera, and cell-based assays and the Attana Virus Analytics (AVA) platform, a proprietary in vitro diagnostics (IVD) tool. (cision.com)
Education in advanced Attana cell-based assays and practical hands-on training in maintenance. (cision.com)
Last week four different clinical groups at one of our key customers were trained in performing biological relevant assays using primary cells. (cision.com)
Primarly with blood and sera based assays, but also cell and tissue-based assays. (cision.com)

Researchers at Texas Biomedical Research Institute report they have created a new cell culture model for human alveolar macrophages in the lab. (genengnews.com)
DAMPs released from cells following tissue injury or cell death can be recognized by macrophages and can thus trigger the inflammatory response by different pathways, including TLR [ 14 ]. (biomedcentral.com)

Al-Suwaini AS, Bahkali AH, Hasnain SM: Airborne viable fungi in Riyadh and allergenic response of their extracts. (aaem.pl)
Basilico ML, Chiericatti C, Aringoli EE, Althaus RL, Basilico JC: Infl uence of environmental factors on airborne fungi in houses of Santa Fe City, Argentina. (aaem.pl)
Airborne viable, non-viable, and allergenic fungi in a rural agricultural area of India: a 2-year study at five outdoor sampling stations. (microbiologyresearch.org)
Studies of airborne fungi provide prevalence data that are important to estimate patients' exposures to molds. (medscape.com)

Yellow water soluble MTT is metabolically reduced in viable cells to a blue-violet insoluble formazan. (acmeresearchlabs.in)
The number of viable and non-viable cells was established by manual counting. (bvsalud.org)

We will be treating in vitro airway tissues with synthetic, non-psychoactive derivatives of THC and CBD to determine the benefit or harm of the cannabinoids in the setting of Covid-19 infection. (medstartr.com)
MS or Murashige and Skoog Media is a nutrient media used for the in vitro culture of plant cells, tissues, and organs. (plantcelltechnology.com)
The order shows a market acceptance for our technology and capability to characterize interactions with cells, tissues, viruses etc it also provides a value of our application knowledge and the advises we can provide. (cision.com)

By in vitro selection for binding α2-6 sialosides, we identified four variant viruses with amino acid substitutions in the hemagglutinin (S227N, D187G, E190G, and Q196R) that revealed modestly increased α2-6 and minimally decreased α2-3 binding by glycan array analysis. (nih.gov)

In-Vitro Inhalation Experimentation Design and Dosimetry 11. (prit-systems.de)

A new publication from ISAB and partners has radical implications for the future of toxicology testing, airborne pollution research and IVIVC ( In Vitro In Vivo Collaboration ) the early stage, animal-free testing method that scientists have been searching to realize for years. (inhalation.se)

Overview of Environmental and Occupational Pulmonary Diseases Environmental and occupational pulmonary diseases result from inhalation of dusts, chemicals, gases, fumes, and other airborne exposures. (msdmanuals.com)

To push the frontiers of optical imaging and biological image analysis to quantify the effects of particle uptake on single cells and cell populations by using various types of optical microscopy including confocal, structured illumination, light sheet and fluorescence lifetime imaging. (admin.ch)

Is Bordetella pertussis airborne or droplet? (thisisbeep.com)

The results showed that cell proliferation was similar between the groups (p =0.2174). (bvsalud.org)
It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. (bvsalud.org)
The proliferation of T cells from the lungs or blood when exposed to beryllium in vitro forms the basis of the beryllium lymphocyte proliferation test [BeLPT] test, which is used clinically to identify immune sensitization to beryllium. (msdmanuals.com)

2021). A 3d in vitro model of the human airway epithelium exposed to tritiated water: Dosimetric estimate and cytotoxic effects. (altex.org)
Asthma results from complex interactions among inflammatory cells, their mediators, airway epithelium and smooth muscle, and the nervous system. (medscape.com)

The cell-mediated immunity is measured by using a delayed-type hypersensitivity (DTH) skin test ( 5 ) or an in vitro assay of cellular immunity to Coccidioides spp. (cdc.gov)

Toll-like receptors (TLRs) belong to the family of PRRs and are activated upon binding with several ligands, often referred to as senses pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) [ 7 ] initiating a signaling cascade leading to the release of cytokines and chemokines which recruits immune cells [ 8 ]. (biomedcentral.com)

Written by scientists from Inhalation Sciences AB, Karolinska Institutet and the Bionanotechnology Unit of the Swedish University of Agricultural Sciences in Uppsala, the paper shows how ISAB's cell simulation model, Xpose ALI, could be used to replace animals in toxic air pollutant tests. (inhalation.se)

Such airborne NM are described as "aerosolised" and little is known about the fate of these mixtures and if they may cause adverse health effects. (bund.de)

Prevalence of airborne Aspergillus flavus in Khartoum (Sudan) airspora with reference to dusty weather and inoculum survival in simulated summer conditions. (microbiologyresearch.org)

The complex changes required for airborne transmissibility in ferrets suggest that extensive evolution is needed for H5N1 transmissibility in humans. (nih.gov)

Thus, there remains an unmet need for cost-effective methods for generating and/or differentiating primary cells into a HAM phenotype, particularly important for translational and clinical studies. (genengnews.com)
Methods We treated cell cultures with BC/BL or defined single plant extracts, purified anthocyanins and astaxanthin in different concentrations and subsequently infected the cultures with the Measles virus (wild-type or vaccine strain Edmonston), Herpesvirus 1 or 8, or murine Cytomegalovirus. (uni-wuerzburg.de)
To address this issue this project will use "tailored" reference aerosols, combined with high-resolution optical imaging of exposed cells and state-of-the-art cell analysis methods to study the cytotoxic effects of airborne PM in vitro. (admin.ch)

2022). An inter-laboratory effort to harmonize the cell-delivered in vitro dose of aerosolized materials. (altex.org)

To clarify the possible effects, toxicological examinations are conducted in vitro using various cell systems, as well as in in vivo studies (with mice). (bund.de)
We have sufficient quantities of research-grade material for the in vitro studies and eventually for the in vivo studies. (medstartr.com)

Endotoxins are usually measured in samples of airborne or settled dust. (bmj.com)
Measures to control construction-associated dust have been codified into law, but no data exist to demonstrate the efficacy of these mandatory, dust-control measures in eliminating airborne arthroconidia or associated coccidioidal infections. (cdc.gov)
There were also significant differences in microbial composition in dust samples and in innate immune cells between the two groups of children. (medscape.com)

2017). Comparison of cellular and transcriptomic effects between electronic cigarette vapor and cigarette smoke in human bronchial epithelial cells. (altex.org)

Though antibiotics are a traditional solution to the problem, culturists now prefer to use PPM in the media that provide their plants an all-round protection from all types of contamination, such as airborne, waterborne, or those coming from other sources. (plantcelltechnology.com)

AREG intranasal treatment for 3 days during recovery phase reduced repetitive DE-induced airway inflammatory cell influx and cytokine release. (cdc.gov)

The Japanese team uses Ushio's Care222 krypton-chloride excimer lamp against viral cultures of SARS-CoV-2 in an in vitro experiment. (studyfinds.org)

Members of the institute have published in a wide range of peer-reviewed journals, including Nature, Molecular Cell, Angewandte Chemie, JACS, Science Advances, Proceedings of the National Academy of Sciences USA, Nucleic Acids Research, Chemical Science and many others. (le.ac.uk)

6. Effner, R., J. Hiller, S. Eyerich, C. Traidl-Hoffmann, K. Brockow, M. Triggiani, H. Behrendt, C. B. Schmidt-Weber and Buters J . Cytochrome P450s in human immune cells regulate IL-22 and c-Kit via an AHR feedback loop. (zaum-online.de)
As with the other highly pathogenic coronaviruses, SARS and MERS, it is able to breach the normally protective layers of the nose and throat and disable the underlying immune cells. (medstartr.com)

The QuantiFERON®-TB Gold Plus assay is an in vitro diagnostic test using a peptide cocktail simulating ESAT-6 and CFP-10 proteins to stimulate cells in heparinized whole blood. (cdc.gov)

2. Candeias J, Schmidt-Weber C, Buters J. Dosing intact birch pollen grains at the air-liquid interface (ALI) to immortalized human bronchial epithelial cell line BEAS-2B. (zaum-online.de)

Elemental analysis of vapour was done by mass spectrometry, and oxidative potential of vapour assessed by antioxidant depletion in vitro . (ersjournals.com)

TB is an airborne disease caused by Mycobacterium tuberculosis. (cision.com)

Many experimental techniques are available: from standard instrumentation for size and concentration measurements of airborne nanoparticles to more specialized equipment. (lu.se)

It is critical to study tissue-specific cells to better understand mechanisms of health and disease, and to screen potential new therapies," said Texas Biomed professor Larry Schlesinger, MD, and senior author of the paper. (genengnews.com)
The study adds that 254-nm light can break through the outer layer of dead cells on the skin and eyes, irradiating human tissue. (studyfinds.org)

Is pertussis an airborne infection? (thisisbeep.com)
Liebers said clinicians should be alerted to "the big three" signs of secondary infection - fever, elevated white blood cell count, and lactic acidosis. (the-hospitalist.org)
A facility's airborne-infection isolation rooms may become filled to capacity, but that isn't critical, Liebers said. (the-hospitalist.org)

Previous studies on the impact of CyaA on human epithelial cells have been carried out using cell lines derived from the airways or the intestinal tract. (uni-wuerzburg.de)
The project will work toward improved, validated protocols for harmonising/standardising cell analysis studies, as well as on the integration of multi-omic approaches for statistical analysis of complex data sets on a European level. (admin.ch)

They boost the growth of plants or help in reducing the time plant cells take to start dividing in vitro environments. (plantcelltechnology.com)

We hypothesised that a mixture of Ribes nigrum L. (Grossulariaceae) (common name black currant (BC)) and Vaccinium myrtillus L. (Ericaceae) (common name bilberry (BL)) extracts (BC/BL) with standardised anthocyanin content as well as single plant extracts interfered with the replication of Measles virus and Herpesviruses in vitro. (uni-wuerzburg.de)

A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
The QuantiFERON®-TB Gold Plus assay tests for Cell Mediated Immune (CMI) responses to peptide antigens that simulate mycobacterial proteins. (cdc.gov)

Chemicals and Drugs6

Information Science1

Particles11

Toxicol In Vitro2

Particulate matter5

Data from in vitro1

Cytotoxicity8

Exposure10

Characterization2

Spores4

Allergens2

Endotoxin2

Toxicity2

Lung12

Pathogens4

Respiratory8

Assays6

Macrophages2

Fungi4

Viable2

Tissues3

Viruses1

Dosimetry1

Toxicology1

Dusts1

Particle1

Droplet1

Proliferation3

Airway epithelium2

Delayed-type hypersensit1

Cytokines1

Inhalation1

Mixtures1

Prevalence1

H5N11

Methods3

20221

Vivo studies2

Dust3

20171

Contamination1

Inflammatory1

Cultures1

Molecular1

Immune cells2

Diagnostic1

Epithelial cell1

Oxidative potential1

Mycobacterium1

Concentration1

Study2

Infection3

Studies2

Environments1

Plant extracts1

Proteins2