1983) used a murine monoclonal antibody (A3D8) to identify an erythrocyte antigen inhibited by the In(Lu) gene. (wdv.com)
After protein quantification, 500 μg of each cell lysis was added with 1 μg of CD44 monoclonal antibody and shaken at 4°C overnight, then added with 40 μL of Protein A-agarose and shaken at 4°C for 2 h, finally centrifuged at 2500 rpm for 5 min and washed to collect the precipitation. (pdesignal.com)
Lewis y monoclonal antibody (1:1000) was used to detect Lewis y antigen. (pdesignal.com)
Platelet aggregation2
This process result in neutrophils infiltration, hemorrhage , fibrin deposition and platelet aggregation. (wikidoc.org)
This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin -like molecules. (wikidoc.org)
19971
1997. The Leukocyte Antigen FactsBook Academic Press. (elabscience.com)
CD251
The newly arrived CLP cells are CD4−CD8−CD44+CD25−ckit+ cells, and are termed early thymic progenitor (ETP) cells. (wikipedia.org)
Tumor4
T RM responses against tumor/self-antigens can concurrently result in the development of pathogenic T RM responses to self, with a growing number of autoimmune diseases and inflammatory pathologies being attributed to T RM responses. (frontiersin.org)
While innate immune cells are important for early tumor immune surveillance, T cells are fundamentally recognized for their crucial role in the antigen-specific recognition and elimination of malignantly transformed cells ( 2 ). (frontiersin.org)
In addition, tumor-associated antigens (TAAs) produced by irradiated tumor cells can be captured by antigen-presenting cells (APCs) in the TME and presented to T cells. (nature.com)
[4] BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R (BR3), TACI (transmembrane activator and calcium modulator and cyclophilin ligand interactor), and BCMA (B-cell maturation antigen), all of which have differing binding affinities for it. (wikidoc.org)
Signaling1
Genetic inactivation of the machinery or re-introduction of ANKRD52 frequent patient mutations dampens the JAK-STAT-interferon-γ signaling and antigen presentation in cancer cells, largely by abolishing miR-155-targeted silencing of suppressor of cytokine signaling 1 (SOCS1). (nature.com)
1989) demonstrated that the lymphocyte homing receptor is identical to the human leukocyte surface glycoprotein called CDw44, on the basis of studies at the Third International Workshop on Human Leukocyte Differentiation Antigens. (wdv.com)
1989)' 'demonstrated' 'sameness' 'the human leukocyte surface glycoprotein' 'is called' 'CDw44' 'studies' 'were conducted at' 'the Third International Workshop on Human Leukocyte Differentiation Antigens. (wdv.com)
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. (elabscience.com)
Cells7
In the early 2000's, large and persistent populations of antigen (Ag)-specific CD8 T cells in peripheral tissues were initially classified as T EM cells in recirculation from the blood ( 8 , 11 ). (frontiersin.org)
1A29 mAb inhibits Phorbol 12-Myristate 13-Acetate (PMA)-induced aggregation of phytohemagglutinin (PHA)-stimulated splenic blasts, as well as the adhesion of mitogen-stimulated blasts to high endothelial venule (HEV) cells and purified ICAM-1. (bdbiosciences.com)
As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. (elabscience.com)
High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. (elabscience.com)
CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. (elabscience.com)
NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
The cells had fibroblast-like shape and intensively expressed CD90, CD73, CD105, CD44, CD146, and CD117 and were capable of induced adipogenic and osteogenic differentiation in vitro. (endometriosi.it)
Cell3
1990) demonstrated that CD44 is the main cell surface receptor for hyaluronate. (wdv.com)
T cell memory is antigen-specific, and can provide durable host-wide protection. (frontiersin.org)
Thus, CD44 has been reported to be a valuable marker for memory cell subsets. (elabscience.com)
Gene1
Our experiments demonstrate that the specificity of the chamber surface antigen Mta is gthroughdiscerniblened connecttly aside the cytoplasmic gene Mtf and the chromosomal gene Hmt. (upb.ro)
Receptor3
2. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. (nih.gov)
CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. (thermofisher.com)
The Ezrin-Radixin-Moesin (ERM) proteins, which link plasma membrane proteins with the actin cytoskeleton and regulate the steady-state diffusion dynamics of the B cell receptor (BCR), are transiently dephosphorylated upon antigen receptor stimulation. (silverchair.com)
Isoforms4
8. CD44 isoforms containing exons V6 and V7 are differentially expressed on mitogenically stimulated normal and Epstein-Barr virus-transformed human B cells. (nih.gov)
Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). (thermofisher.com)
Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. (thermofisher.com)
Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. (thermofisher.com)
Antibody4
The following product was used in this experiment: CD44 Monoclonal Antibody (IM7), NovaFluor™ Blue 510, eBioscience™ from Thermo Fisher Scientific, catalog # M010T02B01, RRID AB_2896788. (thermofisher.com)
NIR-PIT is a molecularly targeted phototherapy for cancer that is based on injecting a conjugate of a near-infrared, water-soluble, silicon-phthalocyanine derivative, IRdye700DX (IR700), and a monoclonal antibody (mAb) that targets an expressed antigen on the cancer cell surface. (nih.gov)
ICD occurs as early as 1 min after exposure to NIR light and results in irreversible morphologic changes only in target-expressing cells based on the newly discovered photoinduced ligand release reaction that induces physical changes on conjugated antibody/antigen complex resulting in functional damage on cell membrane. (nih.gov)
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Adhesion molecule4
CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. (biolegend.com)
15. Rheumatoid synovial fibroblasts are stimulated by the cellular adhesion to T cells through lymphocyte function associated antigen-1/intercellular adhesion molecule-1. (nih.gov)
CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component. (thermofisher.com)
ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. (thermofisher.com)
Hyaluronic acid1
Interaction Between Hyaluronic Acid and Its Receptors INTERACTION BETWEEN HYALURONIC ACID AND ITS RECEPTORS (CD44, RHAMM) REGULATES THE ACTIVITY OF INFLAMMATION AND CANCER Topic Editor: David Naor, Hebrew University of Jerusalem, Israel The biological outcome of Hyaluronan (also hyaluronic acid, abbreviated HA) interaction with its CD44 or RHAMM receptors recently attracted much attention within the scientific community owing to a Nature article by Tian X et al. (pdfhost.io)
Lymphocyte3
12. Down's syndrome lymphoid cell lines exhibit increased adhesion due to the over-expression of lymphocyte function-associated antigen (LFA-1). (nih.gov)
14. Lymphocyte function-associated antigen (LFA-1) is involved in B cell activation. (nih.gov)
4. CD4 binding to major histocompatibility complex class II antigens induces LFA-1-dependent and -independent homotypic adhesion of B lymphocytes. (nih.gov)
Lymphoid1
Secondary lymphoid tissues include lymph nodes, spleen, tonsils, and the aggregations of lymphoid tissue located in the gastrointestinal and respiratory tracts. (medilib.ir)
Inhibition1
19. Interleukin-4-mediated aggregation of anti-IgM-stimulated human B cells: inhibition of aggregation but enhancement of proliferation by antibodies to LFA-1. (nih.gov)
Hyaluronan1
13. Regulation of CD44-hyaluronan interactions in Burkitt's lymphoma and Epstein-Barr virus-transformed lymphoblastoid B cells by PMA and interleukin-4. (nih.gov)
Epithelial2
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. (thermofisher.com)
CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. (thermofisher.com)
Interactions1
It plays an important role in pathogen-induced aggregation and/or activation of platelets and can also enhance interactions mediated by GPIIb/IIIa and GPIbα. (medscape.com)
Enzyme1
This protein aggregation facilitates recruitment of the cysteine-aspartic acid enzyme pro-caspase-8, which becomes active upon dimerization. (biomedcentral.com)
Expression2
CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. (thermofisher.com)
Disruption of ERM protein function using dominant-negative or constitutively active ezrin constructs or knockdown of ezrin and moesin expression quantitatively and qualitatively alters BCR microcluster formation, antigen aggregation, and downstream BCR signal transduction. (silverchair.com)
Cell surface1
The article provides evidence that interaction of long tissue HA (6000-12,000 kDa) of the naked mole rat with cell surface CD44, in contrast to the interaction of short tissue HA (less than 3000 kDa) with the mouse CD44, makes the difference. (pdfhost.io)
Mouse1
Pre-lysed human blood leukocytes were stained with CD44 (clone BJ18) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). (biolegend.com)
Memory1
Thus, CD44 has been reported to be a valuable marker for memory cell subsets. (biolegend.com)
Ensure1
The thymocyte must also ensure that it does not react adversely to "self" antigens, called negative selection. (wikipedia.org)