AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceNamed GroupsHealth Care
Polymerase Chain ReactionClinical Laboratory TechniquesGelsLaboratoriesChromatography, High Pressure LiquidChromatography, AffinitySepharoseChromatography, GelChromatographyElectrophoresis, Agar GelChromatography, Ion ExchangeChromatography, GasChromatography, LiquidChromatography, Thin LayerChromatography, AgaroseLaboratories, HospitalMolecular WeightAnimals, LaboratoryMolecular Sequence DataChromatography, DEAE-CelluloseElectrophoresis, Polyacrylamide GelElectrophoresis, Gel, Two-DimensionalMedical Laboratory PersonnelKineticsAmino Acid SequenceMass SpectrometryIsoelectric FocusingHydrogen-Ion ConcentrationBase SequenceGas Chromatography-Mass SpectrometryElectrophoresis, Gel, Pulsed-FieldEscherichia coliClinical Laboratory Information SystemsElectrophoresisAmino AcidsSubstrate SpecificityChromatography, Reverse-PhaseChromatography, PaperCattleMethodsSensitivity and SpecificityRabbitsDNATemperatureChemistryReproducibility of ResultsQuality ControlChemical PhenomenaImmunoelectrophoresisDNA, BacterialTime FactorsSpecies SpecificityReference StandardsImmunodiffusionLiverProtein BindingLaboratory PersonnelCulture MediaChemical FractionationSpectrophotometry, UltravioletIndicators and ReagentsCloning, MolecularCell LineTandem Mass SpectrometryPeptide FragmentsDrug StabilityBacterial ProteinsRecombinant ProteinsPolysaccharidesTrypsinEvaluation Studies as TopicCarbohydratesPlasmidsCentrifugation, Density GradientLaboratory Proficiency TestingPeptidesCells, CulturedChemical PrecipitationLaboratory Animal ScienceChemistry, ClinicalSwineIsoelectric PointHydrolysisSpectrometry, Mass, Electrospray IonizationUltracentrifugationBinding SitesMagnetic Resonance SpectroscopyMacromolecular SubstancesSpecimen HandlingHot TemperatureBiological AssayIsoenzymesRadioimmunoassayHydroxyapatitesMicroscopy, ElectronCarbohydrate SequenceOligosaccharidesSolubilityProteinsImmunoassay
ElectrophoresisThin Layer ChromatoExchange chromatographyAffinityProteinsDialysisDigestionBiotechnologyPolyacrylamideNucleicHysteresisBiomoleculesAnalyticalBiotechPolymerChromatographicLife science laboratoryDetectionMacromoleculesChainsOrganicRRNAResearchersStrengthMolecularInvolvesAdvantagesAsepticMethodApplicationsBiochemicalPagePowderMutagensMutationsMutagenesisRecent developmentsAnalysisResearchProcessesPaperMediaRangeEquipmentChemicalLiquidLinearGoodLargeMediumMelting temperatureEffect
Electrophoresis16
  • Simply mix with water, gently heat to dissolve the powder blend to quickly prepare reliable agarose gels for electrophoresis. (sigmaaldrich.com)
  • Agarose is non-toxic and has several properties and specifications that make it useful as a gelling agent in many applications, such as nucleic acid electrophoresis, immunodiffusion techniques, gel plates or overlays for cells in tissue culture, cell culture media, gel chromatography, affinity chromatography, and ion exchange chromatography. (sigmaaldrich.com)
  • Gel electrophoresis is a common life science laboratory technique used to extract biological molecules based on their size, such as in DNA separation and DNA detection. (sigmaaldrich.com)
  • Techniques include protein isolation, purification and characterization, protein assays, immunoassay: Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot, Sodium Dodecyl Sulfate Polyacrylamide (SDS-PAGE), agarose gel electrophoresis, and Polymerase Chain Reaction (PCR) technology. (elac.edu)
  • Agarose is often used in biochemistry laboratories as a semi-solid support in techniques such as electrophoresis and chromatography for the separation and analysis of biological macromolecules or small molecules. (ginobiotech.com)
  • Furthermore analytical methods like SDS-PAGE, thin layer chromatography, Agarose gel electrophoresis, ELISA and turbidimetry, enzymatic analytic, Bradford protein assay , Biuret protein assay and BCA assay are used. (hs-esslingen.de)
  • This network is based on characterization of whole bacterial genomes by using macrorestriction digestion patterns that are separated by pulsed-field gel electrophoresis (PFGE), a technique that has emerged as a common standard for subtyping EHEC O157 isolates ( 6 ). (cdc.gov)
  • At this download chemical, the Molecules on the microparticle are overrepresented by Acts in the gel-electrophoresis. (arm-sind-die-anderen.de)
  • The Bio-Rad Explorer program offers a selection of hands-on classroom activities designed for students at every level, whether they are just starting to learn about DNA or diving into the real-world applications of gel electrophoresis, PCR, or even real-time quantitative PCR (qPCR) analysis. (bio-rad.com)
  • These educational DNA analysis kits utilize hands-on techniques to explore DNA structure and function, restriction digestion, and agarose gel electrophoresis. (bio-rad.com)
  • Use pre-amplified DNA samples (no thermal cycler needed) and gel electrophoresis to deduce the mode of transmission and chain of infection of a virus throughout a restaurant. (bio-rad.com)
  • Showcase how science, technology, engineering, and mathematics (STEM) are used together to develop tools and solve problems through the building of a horizontal gel electrophoresis chamber. (bio-rad.com)
  • Engage your students with real-world relevance through this hands-on, inquiry- based laboratory designed to run with the IDEA Kit - Inquiry Dye Electrophoresis Activity . (bio-rad.com)
  • Introduce a key biotechnology technique or demonstrate the components and critical properties of gel electrophoresis. (bio-rad.com)
  • Colorful and visible results prompt further inquiry and discussion into gel electrophoresis. (bio-rad.com)
  • For DNA concentration samples, the use of resin binding columns and gel electrophoresis with subsequent gel extraction can concentrate nucleic acid samples. (gbiosciences.com)
Thin Layer Chromato4
Exchange chromatography1
Affinity12
  • Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. (wikipedia.org)
  • Affinity chromatography is useful for its high selectivity and resolution of separation, compared to other chromatographic methods. (wikipedia.org)
  • Affinity chromatography has the advantage of specific binding interactions between the analyte of interest (normally dissolved in the mobile phase), and a binding partner or ligand (immobilized on the stationary phase). (wikipedia.org)
  • In a typical affinity chromatography experiment, the ligand is attached to a solid, insoluble matrix-usually a polymer such as agarose or polyacrylamide-chemically modified to introduce reactive functional groups with which the ligand can react, forming stable covalent bonds. (wikipedia.org)
  • page needed] Affinity chromatography does not require the molecular weight, charge, hydrophobicity, or other physical properties of the analyte of interest to be known, although knowledge of its binding properties is useful in the design of a separation protocol. (wikipedia.org)
  • Types of binding interactions commonly exploited in affinity chromatography procedures are summarized in the table below. (wikipedia.org)
  • The ligands used in affinity chromatography are obtained from both organic and inorganic sources. (wikipedia.org)
  • Affinity chromatography can be used in a number of applications, including nucleic acid purification, protein purification from cell free extracts, and purification from blood. (wikipedia.org)
  • By using affinity chromatography, one can separate proteins that bind to a certain fragment from proteins that do not bind that specific fragment. (wikipedia.org)
  • This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins. (sigmaaldrich.com)
  • This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products. (sigmaaldrich.com)
  • Recombinant proteins commonly incorporate N- and C-terminal tags, which are small sequences that are used for immunohistochemical detection and purification, or protein affinity chromatography, depending on the specific N- and C-terminal tag and intended downstream application. (sigmaaldrich.com)
Proteins6
Dialysis1
  • The kits use an array of techniques, including dialysis, resin binding and gel filtration to remove unwanted reagents from the nucleic acid samples. (gbiosciences.com)
Digestion2
  • The melting point of agarose is between 62 and 65°C, and it can be maintained in a liquid state for several hours at 37°C after melting and solidifies into gel at 30°C. It is mostly used for in situ digestion of chromosomal DNA and DNA fragment recovery within the gel. (ginobiotech.com)
  • Students learn about the variety of digestion and degradation techniques in a range of experiments. (hs-esslingen.de)
Biotechnology2
  • The application of industry standard communication protocols, Food and Drug Administration (FDA) regulations, and Standard Operating Procedures (SOP) in the laboratory makes it easier for students to transition into the workforce in the biotechnology industry. (elac.edu)
  • In this recent review published in the journal Gels, iBB researchers Filipe Miguel (MSc student in Biotechnology), Frederico Barbosa (PhD student in Bioengineering), Prof. Frederico Ferreira and Dr. João Silva highlight the most common conductive materials combined with hydrogels and their diverse applications, and discuss the current state of research on the development of electrically conductive hydrogels for cartilage tissue engineering. (scoop.it)
Polyacrylamide1
  • Electron TransferHeterogenous Lordship gel among unvarnished analyzers of quantification II in practice capacity Captive II polyacrylamide isolates from equal very-low-density methods of intestine was ultracentrifuged in clever exosomes of silt captcha. (democo.de)
Nucleic4
  • The nucleic acid analysis is increasing in the field of bioscience and most preferable application in diagnostic laboratories at commercial scale. (ajpaonline.com)
  • The paper highlights the recent developments of laboratory methods in nucleic acid analysis and their commercial paradigm is necessary in current era of developmental perspectives in diagnosis field. (ajpaonline.com)
  • Nucleic Acid Analytical Techniques - Current era of Development Perspectives in Diagnosis Field. (ajpaonline.com)
  • GET™ AGAROSE DNA method involves release of nucleic acid fragments from gel pieces followed by capture of nucleic acids on the GET™ Spin Columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer. (gbiosciences.com)
Hysteresis1
  • Agarose solutions exhibit hysteresis when transitioning from liquid to gel. (sigmaaldrich.com)
Biomolecules1
  • This course provides fundamental concepts and techniques to separate and analyze chemical mixtures, such as biomolecules. (elac.edu)
Analytical3
  • To impart hands on training in basic as well as advanced techniques in the Biochemical and Analytical sciences. (curaj.ac.in)
  • We also reveal using a combination of immunological techniques and proteomics analytical tools that the PDE10A isoform X4 is present in the area of the developing acrosome of spermatids and of the acrosome of mature spermatozoa. (plos.org)
  • Kendro Laboratory Products offers analytical instruments, lab equipment and industrial equipment. (biosciregister.com)
Biotech3
  • As a professional and responsible agarose powder company in China, Gino Biotech provides food systems solutions to many industries, at the same time, flexible packaging solutions could also be provided. (ginobiotech.com)
  • As one leading agarose supplier & manufacturer in China, Gino Biotech will always be your good partner for agarose powder and other hydrocolloids from China. (ginobiotech.com)
  • Gino Biotech is a very good agar powder and agarose supplier, they are very professional and patient. (ginobiotech.com)
Polymer1
  • The linear agarose polymer forms chains which create fibers that are flexible and form a web of channels with diameters from 50 nm to 200 nm, depending on the amount of agarose used. (sigmaaldrich.com)
Chromatographic2
  • These chromatographic processes are known as periodic counter-current chromatography (PCC). (wikipedia.org)
  • Students learn about different chromatographic techniques and how to operate the Äkta system. (hs-esslingen.de)
Life science laboratory1
Detection1
  • We reasoned that it would be worthwhile to establish a generally applicable reverse genetic technique based on an unbiased and practicable random mutagenesis and an efficient mutation-detection performed on standard laboratory equipment. (biomedcentral.com)
Macromolecules1
  • In the Biochemistry Laboratory the students learn and practise the basic techniques of handling with biological molecules and macromolecules, which playing an inportant role within living organism. (hs-esslingen.de)
Chains1
  • The chemical structure of agarose is composed of long chains of alternating 1,3-linked β-D-galactose and 1,4-linked 3,6-endoether-L-galactose. (ginobiotech.com)
Organic1
  • Agarose is an organic substance with the chemical formula C24H38O19, a white or yellow bead-like gel particle or powder, which is a linear polymorph. (ginobiotech.com)
RRNA2
  • Bacteria in a dust event was analyzed using the 16s rRNA sequencing technique. (aaqr.org)
  • In addition, studies on gut microbiota and serum lipidomics based on 16S rRNA sequencing and ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) were conducted to elucidate the therapeutic mechanism of NAFLD in QHD. (frontiersin.org)
Researchers1
  • This paper provides a comprehensive overview of the various techniques and workflows available to researchers today in the field of molecular breeding, and how these tools complement the ones already used in traditional breeding. (hindawi.com)
Strength1
  • Gel strength - The force that must be applied to the gel to fracture it. (sigmaaldrich.com)
Molecular2
  • Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. (hindawi.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
Involves1
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Advantages1
  • Both undirected and directed reverse genetic techniques have certain advantages and drawbacks. (biomedcentral.com)
Aseptic2
  • Other laboratory skills include growing, maintaining, staining, and identifying the morphology of bacteria, in which aseptic techniques are emphasized. (elac.edu)
  • This includes the production of media, the aseptic technique and the preparation of precultures and main cultures in bioreactors. (hs-esslingen.de)
Method2
  • by not, physical other disorders were collected to prevent often 40)(41)(42)(43 with equivalent ebook, and links became However less first, interfering famous to choice laboratories and inlineView( 54), which presented to s number by the acetate charge Man( method Download email in new tabFigure 1. (democo.de)
  • For download chemical separations, at the high minimal mass, effect could run polyanions with the actually inflated mL laboratories of executive method, ionizable transport, and acid customers automated in a content. (arm-sind-die-anderen.de)
Applications1
  • We provide a wide array of agarose products with varying gel strengths, melting temperatures, gelling temperatures, and electroendosmosis (EEO) levels to accommodate all of your research applications. (sigmaaldrich.com)
Biochemical1
  • The laboratory work undertaken by the students teaches them how to isolate micro-organisms from various sample materials, how to prepare pure cultures and perform the phenotypic characterisation of the isolates produced by means of microscopic, biochemical and physiological methods. (hs-esslingen.de)
Page1
Powder3
  • What is Agarose powder? (ginobiotech.com)
  • We import agar powder and agarose powder from China every month. (ginobiotech.com)
  • We're a distributor in Ukraine, we import food ingredients including agar & agarose powder from China to Ukraine every month. (ginobiotech.com)
Mutagens1
  • During and directly following the Second World War, radiation-based techniques were complemented by chemical mutagens that were less destructive, freely available, and easier to work with. (hindawi.com)
Mutations2
  • Mutations within the gene are induced and identified by various techniques and only subsequently is the mutant phenotype analyzed [ 1 ]. (biomedcentral.com)
  • This genetic erosion eventually became a bottleneck and various techniques to induce mutations and artificially increase variation emerged in the middle of the last century [ 9 ]. (hindawi.com)
Mutagenesis1
  • Thus, MOs are important counterparts to current mutagenesis and genome editing techniques. (cdc.gov)
Recent developments1
  • The high-resolution techniques are minimize the biological barriers in recent developments and focusing on independent structure. (ajpaonline.com)
Analysis3
  • Multilocus variable number tandem repeat analysis (MLVA) is a subtyping technique for characterizing human pathogenic bacteria such as enterohemorrhagic Escherichia coli (EHEC) O157. (cdc.gov)
  • The Syngene G:Box iChemi XR Gel Documentation and Analysis System (GelDoc, Gel Imager) allows simple and easy acquisition of data from fluorescence, chemiluminescent and visible light assays. (bridgepathscientific.com)
  • Teach your students the core techniques used for DNA analysis and visualization. (bio-rad.com)
Research1
  • Later, more sophisticated techniques such as gamma and neutron radiation were developed at newly established nuclear research centers. (hindawi.com)
Processes2
  • To achieve optimum management of fed-batch processes of bioreactors, the students design feeding strategies and examine them experimentally in laboratory bioreactors. (hs-esslingen.de)
  • For the destruction of PAH contaminants, bioremediation is considered a better process than chemical or physical remediation processes because the technique is economical and efficient [ 5 ]. (omicsonline.org)
Paper2
  • The basis of this determination were to be laboratories of impossible paper in news and &, by comprising four isoelectric F(+)RNA science laboratories( IV, I have electrical Special elution and II, III are significant Original plasma). (democo.de)
  • So, the download chemical of number of any liver through the seizure spins on its T for the pH gemeinsamen, its paper of oxidation, and the Chromatography and camera of complicating results in the comparison. (arm-sind-die-anderen.de)
Media1
  • The laboratory component provides to skills to solution and media preparation, which is achieved through calculating, assembling, and performing proper laboratory techniques. (elac.edu)
Range1
  • Pacific Combustion Engineering offers a range of laboratory equipment. (biosciregister.com)
Equipment2
  • Under the direction and guidance of qualified professors and academicians, as well as experienced laboratory technologists, students are exposed to a variety of laboratory equipment and skills at UCSI University . (eduspiral.com)
  • United Laboratory Plastics manufactures laboratory supplies and equipment. (biosciregister.com)
Chemical1
  • Agarose is divided into general agarose and low-melting agarose with a reduced melting point after chemical modification. (ginobiotech.com)
Liquid2
  • Electroendosmosis (EEO) - Refers to the transfer of liquid through a gel. (sigmaaldrich.com)
  • Reduce plastic waste and eliminate hazardous liquid waste for more sustainable laboratories with GenElute™-E Single Spin DNA and RNA prep kits. (sigmaaldrich.com)
Linear1
  • Agarose is a linear polymorph and agaropectin is a heterogeneous mixture of many smaller molecules. (ginobiotech.com)
Good1
  • Gino is indeed a good agarose supplier, we appreciate their work. (ginobiotech.com)
Large2
  • However, the technique is time-consuming and technically challenging and hence not applicable on a large scale. (biomedcentral.com)
  • Bio Cold Scientific Inc. designs & manufactures laboratory and chromatography refrigerators, freezers, large capacity reach-in incubator/stability chambers & environmental rooms & chambers. (biosciregister.com)
Medium2
  • Binding to the solid phase may be achieved by column chromatography whereby the solid medium is packed onto a column, the initial mixture run through the column to allow settling, a wash buffer run through the column and the elution buffer subsequently applied to the column and collected. (wikipedia.org)
  • Agarose gels are gels prepared with agarose as a supporting medium. (ginobiotech.com)
Melting temperature1
Effect1
  • Using voltage-clamp techniques, the authors examined the effect of two general anesthetics (ketamine and halothane) on a rat brain potassium channel of known amino acid sequence, and further assessed whether the inhibition of the channel is altered by a partial deletion of the C-terminal sequence of this channel. (silverchair.com)