• A sialic-acid-specific lectin from ovine placental cotyledons was purified by affinity chromatography on bovine submaxillary mucin-agarose followed by gel filtration, and it showed a molecular weight of 65,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis. (illinois.edu)
  • To be able to gather a purified proteins remedy of CST4 extremely, both Ni-nitrilotriacetic acidity affinity chromatography and anion exchange chromatography had been used using 500 mL of cell tradition filtrate (previously gathered and filtered). (informationalwebs.com)
  • Cytosolic fraction was separated from the crude S.digitata worm homogenate by ultracentrifugation at 100,000 g and subjected to ammonium sulfate precipitation followed by affinity chromatography using GSH-agarose column. (bvsalud.org)
  • Purify affinity-tagged proteins, monoclonal antibodies, and other biomolecules with a range of affinity resins including immobilized metal affinity chromatography (IMAC), Protein A, and activated resins. (bio-rad.com)
  • This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins. (sigmaaldrich.com)
  • This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products. (sigmaaldrich.com)
  • Recombinant proteins commonly incorporate N- and C-terminal tags, which are small sequences that are used for immunohistochemical detection and purification, or protein affinity chromatography, depending on the specific N- and C-terminal tag and intended downstream application. (sigmaaldrich.com)
  • Additional purification steps may include crystallization or charcoal filtration. (jinpanbio.cn)
  • Cell tradition moderate was collected and filtered through a 0 then.22 m filtration system membrane for CST4 purification. (informationalwebs.com)
  • The purification scheme involved precipitation with ammonium sulphate, gel filtration and blue-Sepharose chromatography. (ox.ac.uk)
  • Final purification was achieved by gel filtration on 2 or 4% agarose or ion exchange chromatography on diethylaminoethyl cellulose. (elsevierpure.com)
  • Explore a complete line of chromatography resins and media with various base bead and ligand chemistries for your lab-scale and bioproduction purification needs. (bio-rad.com)
  • From high-throughput resin screening to small-scale method development and scale-up optimization, find chromatography resins in prepacked formats for the entire purification development cycle. (bio-rad.com)
  • This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva. (sigmaaldrich.com)
  • particulate disordered structures, e.g., a flocculent precipitate usually consisting of particles with large geometrical anisotropy, such as in V2O5 gels and globular or fibrillar protein gels. (wikipedia.org)
  • Note: Examples of xerogels include silica gel and dried out, compact macromolecular structures such as gelatin or rubber. (wikipedia.org)
  • This New OneTouch Plus Spot Picker excises bands from polyacrylamide or agarose 2D gels and dispenses them into microtitre plates or tubes in one motion. (harvardapparatus.com)
  • Bovine albumin CAS No.: 9048-46-8 Purity: ≥98% (agarose gel electrophoresis) Storage Temperature: 2-8°C Biochem/physiol Actions: Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. (thomassci.com)
  • Beginning with harvest of material from a bioreactor, downstream processing removes or reduces contaminants to acceptable levels through several steps that typically include centrifugation, filtration, and/or chromatographic technologies. (bioprocessintl.com)
  • Gel filtration chromatographic studies indicated that the protein has a native molecular mass of about 54 kDa. (bvsalud.org)
  • In this way, gels are a dispersion of molecules of a liquid within a solid medium. (wikipedia.org)
  • Purify charged and polar molecules with anion and cation exchange chromatography (AEX, CEX) resins available in various particle sizes, ionic forms, and binding capacities. (bio-rad.com)
  • Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. (jinpanbio.cn)
  • Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
  • G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. (gbiosciences.com)
  • Colloidal gels have three phases in their lifespan: gelation, aging and collapse. (wikipedia.org)
  • Experience unparalleled selectivity, resolution, yield and ease-of-use for a variety of biomolecules with the unique separation properties of our multimodal chromatography resins. (bio-rad.com)
  • A gel is a semi-solid that can have properties ranging from soft and weak to hard and tough. (wikipedia.org)
  • After confirmation of the dual digestive function, agarose gel electrophoresis, and gene sequencing, 1 gL?1 CST4-pcDNA3.1 was transformed into COS-7 cells by lipofectamine 2000, cultivated in DMEM containing 10% FBS at 37C with 5% CO2 for 72 h. (informationalwebs.com)
  • Comparable amounts of highly purified protein were obtained from normal and hemophilic plasma following the agarose chromatography step. (elsevierpure.com)
  • A gel has been defined phenomenologically as a soft, solid or solid-like material consisting of two or more components, one of which is a liquid, present in substantial quantity. (wikipedia.org)
  • A hydrogel is a network of polymer chains that are hydrophilic, sometimes found as a colloidal gel in which water is the dispersion medium. (wikipedia.org)
  • Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady state, although the liquid phase may still diffuse through this system. (wikipedia.org)
  • A colloidal gel consists of a percolated network of particles in a fluid medium, providing mechanical properties in particular the rise of elastic behaviour. (wikipedia.org)