Affinity purification include antibody antigen. Medical search. Frequent questions

The following product was used in this experiment: APOE Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-114467, RRID AB_2890414. (thermofisher.com)
Anti-human IgG (H L), ReserveAP™-Labeled antibody is an affinity-purified polyclonal antibody to human IgG, both heavy and light chains (whole IgG), made in goat, and labeled with alkaline phosphatase. (thenativeantigencompany.com)
Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SIN3A Polyclonal Antibody at 4°C overnight. (receptors.org)
CRB offers a range of polyclonal antibody service categories to suit all budget levels and custom antibody variations. (crbdiscovery.com)
SF20 antibody LS-C331255 is an unconjugated rabbit polyclonal antibody to SF20 (MYDGF) from human. (lsbio.com)
Aves' chicken polyclonal antibody services provide a perfect solution: a unique species with expert scientists and know-how! (aveslabs.com)
Our expert team has over two decades of experience in chicken polyclonal antibody production and has a deep understanding of the advantages of using avian IgY to study mammalian systems. (aveslabs.com)
Chicken polyclonals really are the most humane method for polyclonal antibody production. (aveslabs.com)
Rabbit Anti AU1 Tag Polyclonal Affinity Purified from Innovative Research is a polyclonal antibody in a Liquid format, buffered in Phosphate buffered saline, pH 7.2, 0.1% sodium azide. (innov-research.com)

By coupling liquid chromatography with a variety of other immunodetection techniques such as serological proteome analysis (SERPA), it is possible to analyze the hydrophobicity, PI, relative mass, and antibody reactivity of antibodies within a given serum. (wikipedia.org)
The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. (abcam.com)
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Purified by affinity chromatography. (thenativeantigencompany.com)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. (prosci-inc.com)
The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions. (biolegend.com)

For improved specificity, greater sensitivity and lower background cross-adsorbed secondary antibodies are often used. (thenativeantigencompany.com)
Cross-adsorption of secondary antibodies, via affinity purification, eliminates cross-reactivity and improves antibody specificity. (thenativeantigencompany.com)
We are pleased to offer range of secondary antibody options available for purity level, specificity and label type no matter what the application. (thenativeantigencompany.com)
Specificity These antibodies recognize Group A Streptococci. (qedbio.com)
Specificity This antibody recognizes human, mouse, and rat TACE, and 80-130kDa bands are detected in immunoblots. (qedbio.com)
This virus undergoes epochal evolution with periodic emergence of variants with new antigenic profiles and altered specificity for histo-blood group antigens (HBGA), the determinants of cell attachment and susceptibility, hampering the development of immunotherapeutics. (bvsalud.org)
Debate and Outcomes The affinity and specificity from the anti-GCA MK-2894 mAb were explored using indirect competitive ELISA. (eprf.ca)
The antibody that recognizes the antigen is referred to as the 'primary' antibody and confers specificity to the assay. (novusbio.com)

To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species. (abcam.com)
The purified recombinant CAV VP1, VP2 and VP3 proteins were then used as antigens in order to evaluate their reactivity against chicken sera using indirect ELISA. (biomedcentral.com)
In an antibody market full of rabbit and mouse antibodies, chicken IgY polyclonals offer the perfect solution for multiplex staining, including the advantage of reduced cross-reactivity with IgG primary antibodies. (aveslabs.com)
The uniqueness and low cross-reactivity of the Prestige Antibodies ® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. (scientificlabs.ie)
population-based studies have suggested that having a cat in the house could decrease the risk of sensitisation and Methods We did a population-based cross-sectional study of asthma.8-11 Simple explanations for this finding include the children (aged 12-14 years), some of whom had symptoms of possibility that families affected by allergy avoid having asthma and bronchial hyper-reactivity. (cdc.gov)

The definitive solution will probably come from monoclonal antibodies ( 4 ) or synthetic molecules such as nucleic acid aptamers. (cdc.gov)
Despite their efficiency, adoptive T-cell therapies show unique toxicities, which are distinct from those seen with conventional chemotherapies, monoclonal antibodies (mAbs), and small-molecule-targeted therapies. (nature.com)
However, these approaches still require costly and time-consuming clonal expansion in order to produce sufficient quantities of monoclonal antibodies to screen for affinity and selectivity to target antigens (see for e.g. (justia.com)
Thorpe, (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications, A. Pinchera et al (ed.s), pp. 475-506). (justia.com)
21:183-87) and monoclonal antibodies (mAbs) as well as drug-linking and drug-releasing properties (Lambert, J. (2005) Curr. (justia.com)
TotalSeq™ anti-mouse Hashtag reagent is a mixture of two monoclonal antibodies conjugated to the same oligonucleotide. (biolegend.com)
In this work, we evaluated a panel of 16 anti-BoNT/A monoclonal antibodies (mAbs) for their ability to inhibit the in vitro activity of BoNT/A1, /A2, and /A3 complex as well as the recombinant LC of A1. (cdc.gov)

Examples of common applications of immunoproteomics include: The isolation and mass spectrometric identification of MHC (major histocompatibility complex) binding peptides Purification and identification of protein antigens binding specific antibodies (or other affinity reagents) Comparative immunoproteomics to identify proteins and pathways modulated by a specific infectious organism, disease or toxin. (wikipedia.org)
The identification of proteins in immunoproteomics is carried out by techniques including gel based, microarray based, and DNA based techniques, with mass spectroscopy typically being the ultimate identification method. (wikipedia.org)
No antibody was detected against non-immunoglobulin serum proteins. (abcam.com)
Antibodies targeting CD markers or other extracellular proteins may be added prior to fixation if the epitope is disrupted by formaldehyde and/or methanol. (cellsignal.com)
With its history as an original manufacturer, Abcepta has a deep and practical understanding of the production process for antibodies, peptides, and recombinant proteins. (abcepta.com)
Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR). (receptors.org)
Antibodies are defense proteins produced by the vertebrate adaptive immune system for the purposes of binding and targeting for clearance of a diverse range of bacteria, viruses, and other foreign molecules (collectively referred to as antigens) (see, for e.g. (justia.com)
Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). (qedbio.com)
TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, L-selectin, and alpha-cleavage of amyloid protein precursor (APP). (qedbio.com)
The results of these ELISAs using truncated recombinant VP2 and VP3 subunit proteins as coating antigen showed that VP2-345N, VP2-396N and VP3-246M gave good immunoreactivity with CAV-positive chicken sera compared to the other subunit proteins. (biomedcentral.com)
As the Fc fragment of the anti-GCA antibody could normally bind to proteins A on the previously reported red-colored bacterias (conjugate) is normally formed because of the combination of proteins A on as well as the Fc portion from the mAb. (eprf.ca)
Numerous types of labels can be used to visualize the antibodies for detection in the flow cytometer depending on the experimental design including nucleic acid dyes, cell viability dyes, polymer dyes, quantum dots, small organic molecules, and fluorescent proteins. (sigmaaldrich.com)
Common molecular biology applications include analysis of recombinantly expressed fluorescent proteins that have induced expression in various cell types to elucidate gene function or for in vivo cell tracking. (sigmaaldrich.com)
MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. (biolegend.com)

Antibodies are typically labeled through direct conjugation (either as commercially available products or conjugation kits allowing the end user to perform the reaction) or through the use of secondary antibodies. (sigmaaldrich.com)
A new proprietary flow cytometry technology allows end users to flexibly mix and match independently selected antibodies and dyes to assemble them in any combination, without the use of conjugation kits or secondary antibodies. (sigmaaldrich.com)

CD62P antibody LS-B3656 is an unconjugated mouse monoclonal antibody to human CD62P (SELP / P-Selectin). (lsbio.com)

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. (abcam.com)
Application Instructions These antibodies have been qualified for use in ELISA to detect Group A Streptococci. (qedbio.com)
This product can be used for many research applications, including ELISA: 2 ug/ml, IF: 10 - 2 ug/ml, IP: 2 ug/mg, WB: 0.5 - 0.05 ug/ml. (innov-research.com)

Affinity proteomics is a high-throughput method of studying the proteome with antibody or other affinity reagents (e.g. aptamers). (wikipedia.org)
See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. (lsbio.com)
With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel ® flow cytometry antibodies and dyes. (sigmaaldrich.com)
The TotalSeq™-A Hashtag reagents require different primers than regular TotalSeq™ antibodies. (biolegend.com)

Various cell lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. (ptglab.com)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. (ptglab.com)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 40x lens. (ptglab.com)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 10x lens. (ptglab.com)
Purchase ready-to-use Flow Cytometry Antibody Dilution Buffer ( #13616 ), or prepare a 0.5% BSA PBS buffer by dissolving 0.5 g Bovine Serum Albumin (BSA) ( #9998 ) in 100 ml 1X PBS. (cellsignal.com)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®555 Beta Catenin antibody (CL555-51067) at dilution of 1:100. (ptglab.com)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 Beta Catenin antibody (CL555-51067) at dilution of 1:100, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta), CoraLite® Plus 488 CD163 antibody (CL488-16646, green). (ptglab.com)
antibody detection limit dilution 1:32000. (prosci-inc.com)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPS6(GTX113542) antibody at 1:500 dilution. (genetex.com)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RPS6(GTX113542) antibody (Green) at 1:500 dilution. (genetex.com)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with 1131 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). (qedbio.com)
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
The optimal Ig concentration for an application varies by species and antibody affinity. (uiowa.edu)
In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. (uiowa.edu)
Solutions discussed include clarification of data requirements and minimum inhibitory concentration only reporting for regulatory clearance of AST devices, clinical data generation to close breakpoints gaps, advocacy, education, and greater dialogue between stakeholders. (cdc.gov)

A secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody, which directly binds to the target antigen. (thenativeantigencompany.com)
Conventional antibody production has involved the immunization of animals (i.e., mice) with a target antigen, such as a virus, bacteria, foreign protein, or other molecule. (justia.com)
The immunized mice produce on the order of 10 4 -10 5 antibody secreting cells (ASCs), each with the capacity to produce a unique (monoclonal) antibody specific to the target antigen (see, for e.g. (justia.com)
The ASCs are then harvested from the immunized animals and screened in order to select which cells are producing antibodies of desired affinity and selectivity to the target antigen. (justia.com)
Using this method, expansion of each successfully created hybridoma then produces a monoclonal antibody in sufficiently high concentrations to measure its affinity and selectivity to a target antigen. (justia.com)

The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions. (justia.com)
We can also conjugate your antibody to agarose beads for IP, ChIP, or antigen purification applications. (aveslabs.com)
With traditional labeling methods, a basic understanding of the principles of chemical modification is required because the antibody and/or label must be chemically 'activated' before the labeled antibody (or 'conjugate') can be formed. (novusbio.com)

That's why we've invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality. (stratech.co.uk)

The saga of MHC-bound peptides: a renaissance for antigen presentation? (jci.org)
HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)
peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion. (abcepta.com)
Custom peptide services include long peptides (>100 aa), cyclic peptides, difficult sequences, fluorescent labels, phospho-peptides and other post-translational modifications. (abcepta.com)
These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications. (abcepta.com)
These peptides were then used as antigens in indirect ELISAs against chicken sera. (biomedcentral.com)

Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abcepta.com)
As a result of their ability to bind target antigens selectively and with high affinity, antibodies are useful tools for protein purification, cell sorting, diagnostics, and therapeutics. (justia.com)
It implies that the mAb acquired high affinity continuous with GCA. (eprf.ca)
Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. (rndsystems.com)

Enzyme-labeled secondary antibodies, such as HRP or AP, have high catalytic turnover rates and are routinely used in various assays for their rapid and robust signal generation. (thenativeantigencompany.com)
Secondary antibodies conjugated to fluorescent dyes provide brighter signals and multiplexing capabilities in cell analysis and protein analysis applications including immunofluorescence microscopy and cell imaging. (thenativeantigencompany.com)
When detecting low abundance target antigens then enzyme-labeled and dye-labeled secondary antibodies with high sensitivity and signal amplification are very important. (thenativeantigencompany.com)
Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance. (thenativeantigencompany.com)
Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). (receptors.org)
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. (lsbio.com)
Many researchers are starting to use labeled primary antibodies to remove the extra step (and extra background) of secondary antibodies. (aveslabs.com)
Alternatively, using indirect detection, the label is covalently attached to a secondary antibody, which is allowed to bind to the primary antibody during the immunoassay. (novusbio.com)
Excess unbound primary antibody is then washed away and a labeled secondary reagent is added. (novusbio.com)
After a period of incubation (one hour), excess secondary reagent is washed away and the amount of label associated with the primary antibody is quantified. (novusbio.com)
During incubation, the primary antibody dissociates from the antigen with the secondary reagent and in subsequent wash steps, resulting in amplification due to a diminishing amount of primary antibody. (novusbio.com)

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). (ptglab.com)
Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. (bdbiosciences.com)
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. (cytekbio.com)
Immunohistochemistry: LS-B3656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. (lsbio.com)

Identification of such tumor-associated cell surface antigen polypeptides, i.e. tumor associated antigens (TAA), allows specific targeting of cancer cells for destruction via antibody-based therapies. (justia.com)
Recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. (lsbio.com)

By identifying and proceeding to sequence antibodies, scientists are able to identify potential protein targets of said antibodies. (wikipedia.org)
The G44-26 monoclonal antibody specifically binds to the 80-95 kDa glycosylated type I transmembrane protein, CD44, also known as phagocytic glycoprotein-1 (Pgp-1). (bdbiosciences.com)
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)
RPS6 antibody detects RPS6 protein at cytoplasm and nucleus by immunofluorescent analysis. (genetex.com)
There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. (justia.com)
Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. (sigmaaldrich.com)
Prestige Antibodies ® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. (sigmaaldrich.com)
During our years in the field, we have developed novel and proprietary methods for protein sequence analysis and immunogenic peptide determination to maximize immune response and antibody production. (aveslabs.com)
Green Fluorescent Protein, GFP) and staining with fluorescently conjugated antibodies or fluorescent dyes. (sigmaaldrich.com)
Flow cytometry antibodies are used to specifically bind to different cell markers, helping to characterize the cell type and assess any changes in protein expression as part of the experiment. (sigmaaldrich.com)
This antibody recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. (abbomax.com)
This antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein. (abbomax.com)
This antibody recognizes human ALK/p80(pY1282/1283) protein with dual phosphorylation sites Tyrosine 1282/1283. (abbomax.com)
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (genetex.com)

Speak to one of our custom antibody specialists to discuss your next custom antibody project. (prosci-inc.com)
CRB produces a timetable of the programme of events (immunisations & bleed cycles) for each antibody project and this is posted to my account allowing end-users to track the progress of their custom antibody online. (crbdiscovery.com)
Read more about our custom antibody service here . (aveslabs.com)
See the details of our different custom antibody packages here . (aveslabs.com)
Aves Labs represents the best and brightest in custom antibody production, pioneering novel methods in an uncommon species (for antibodies at least). (aveslabs.com)

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. (cytekbio.com)
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. (biolegend.com)

The details of the immunogen for this antibody are not available. (abcam.com)

The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
Aves Labs offers labeling of primary antibodies with biotin and various fluorochromes. (aveslabs.com)

Antibodies without IgE antibody, could be regarded as a modified Th2 to Fel d 1 of the IgG4 isotype were strongly correlated with IgG response. (cdc.gov)
The results for isotype specific antibodies of children in our study were related to evidence of sensitisation, exposure, and the risk of asthma. (cdc.gov)

Cysteine engineered anti-MUC16 antibodies are engineered by replacing one or more amino acids of a parent anti-MUC16 antibody with non cross-linked, reactive cysteine amino acids. (justia.com)
The invention relates generally to antibodies engineered with reactive cysteine residues and more specifically to antibodies with therapeutic or diagnostic applications. (justia.com)
The induced IgG and IgM antibodies were able to stimulate various Fc-mediated effector mechanisms associated with protection against malaria, including phagocytosis, release of reactive oxygen species, production of IFN-γ as well as complement activation and fixation. (nature.com)

Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. (bdbiosciences.com)

Cite Format: Affinity Biosciences Cat# BF0278, RRID:AB_2833916. (affbiotech.com)

Serum antibody identification in particular has proven to be very useful as a diagnostic tool for a number of diseases in modern medicine, in large part due to the relatively high stability of serum antibodies. (wikipedia.org)
The saline method was used to screen and identify IgM-type antibodies in the patient's serum, while the polybrene, anti-globulin, microcolumn agglutination, enzymic and absorption-elution methods were used to screen and identify IgG-type antibodies. (bvsalud.org)
CONCLUSION: It is confirmed that IgM-type anti-E, and IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies exist in the patient's serum by multiple serological methods. (bvsalud.org)

The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al , 1981). (biolegend.com)

TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution . (biolegend.com)
TotalSeq™-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions . (biolegend.com)

Cysteine engineered anti-MUC16 antibodies (Ab) are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered anti-MUC16 antibody-drug conjugates having Formula I: Ab-(L-D)p I where p is 1 to 4. (justia.com)
The use of antibody-drug conjugates (ADC), i.e. immunoconjugates, for the local delivery of cytotoxic or cytostatic agents, i.e. drugs to kill or inhibit tumor cells in the treatment of cancer (Lambert, J. (2005) Curr. (justia.com)

We offer different levels of antibody purity and are experts in phosphospecific antibody purification. (aveslabs.com)

Antibodies are widely used in immunoassays to detect and quantify antigens. (novusbio.com)

Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways: IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. (scientificlabs.ie)

This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells. (cellsignal.com)
This association afforded us a unique opportunity to identify a sarcoidosis-specific antigen recognized by AV2S3+ T cells. (jci.org)
To identify candidates for the postulated sarcoidosis-specific antigen, lung cells from 16 HLA-DRB1*0301pos patients were obtained by bronchoalveolar lavage. (jci.org)
Our focus is on targets covering the major research areas impacting health and disease, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. (abcepta.com)
Genetically engineered T cells expressing a chimeric antigen receptor (CAR) are rapidly emerging a promising new treatment for haematological and non-haematological malignancies. (nature.com)
In current clinical development, targeting CD19 + B cell malignancies, which include acute and chronic B-cell leukaemia and B-cell non-Hodgkin lymphomas (NHLs), with anti-CD19 CAR-T cells is one of the most advanced adoptive T-cell therapies and has been approved by the FDA. (nature.com)
thus, clonal expansion may be achieved by fusing ASCs to murine myeloma (cancer) cells to produce immortalized, antibody-secreting (hybridoma) cells (see, for e.g. (justia.com)
For example, whereas an immune response may produce on the order of 10 4 -10 5 antibody secreting cells, a typical fusion will yield less than 100 viable hybridomas. (justia.com)
Attempts to circumvent hybridoma generation by immortalizing antibody-producing cells using viral transformations have resulted in modest gains in the efficiency of ASC immortalization. (justia.com)
Devices have been developed to estimate the equilibrium dissociation constants of antibodies secreted from single antibody-secreting cells (Story, C. M. et al. (justia.com)
Transmembrane or otherwise tumor-associated polypeptides specifically expressed on the surface of cancer cells as compared to normal, non-cancerous cell(s) have been identified as cellular targets for cancer diagnosis and therapy with antibodies. (justia.com)
ICC/IF analysis of THP-1 cells using GTX55675 IL1 beta antibody. (genetex.com)
The antibody stains restricted regions during the blastoderm stage and intensely stains cells at the ventral furrow. (uiowa.edu)
Expression expands to include almost all cells by the end of gastrulation, but later becomes restricted to, and very strongly expressed in, the developing nervous system. (uiowa.edu)
Contribute to the development of novel antibody sequencing approaches and workflows for both bulk and single cells. (biospace.com)
The red blood cells with specific phenotype were selected for absorption-elution to identify IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies. (bvsalud.org)
Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. (biolegend.com)
The antibodies are specific against mouse CD45 and MHC class I (of a, b, d, j, k, s, and u haplotypes) and can be used to label hematopoietic and non-hematopoietic cells in most commonly used mouse strains for multiplex single cell sequencing analysis. (biolegend.com)

At the core of OmniAb is the ability to engineer animals to generate affinity matured human sequence antibodies for use in conventional and novel therapeutic formats. (biospace.com)

We leverage our proprietary animals with state-of-the-art technology in antigen design, immunization methods and high-throughput single B cell screening to facilitate discovery of best-in-class antibodies. (biospace.com)

The label in an immunoassay provides either 'direct' or 'indirect' detection of the antigen. (novusbio.com)
With direct detection, the label is attached via a covalent bond to the primary antibody. (novusbio.com)
With direct detection, the prior covalent attachment of the label to the primary antibody means that only a single incubation step with the antigen is required and only a single round of wash steps, as opposed to two rounds of incubation and wash steps with indirect detection. (novusbio.com)

For each product, the antibody titer must be optimized for every application by the end user laboratory. (uiowa.edu)

Monomeric ErbB4 binds its ligands with low affinity. (rndsystems.com)
First, there is a period of incubation (one hour) with the unlabeled primary antibody, during which a small fraction of the antibody binds to the antigen. (novusbio.com)

The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. (sigmaaldrich.com)
A large number of the Prestige Antibodies have been used in subcellular localization studies by immunofluorescence (IF) staining of three cell lines: A-431, U-2 OS, and U-251MG. (sigmaaldrich.com)

To find out more about the process of generating a specific antibody please see our Technical section. (crbdiscovery.com)

Abcepta is a leading manufacturer of primary antibodies with more than fifteen years of experience. (abcepta.com)
Undoubtedly, the main reason for this is that direct labeling of primary antibodies is relatively complicated and, historically, antibody labeling has been carried out only by those with specialist knowledge of chemical-modification techniques. (novusbio.com)
the lysine side chain (with a -NH2 terminus, a primary amine) is commonly used to covalently attach labels to antibody molecules. (novusbio.com)

This antibody recognizes epitope 1 of CD44 antigen according to the HLDA workshop studies. (bdbiosciences.com)

We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. (abcepta.com)

Antibody production - Immunisations are carried out using optimised protocols in all available species. (crbdiscovery.com)
We also provide Prestige Antibodies ® protocols and other useful information. (sigmaaldrich.com)
Anyone new to this field will find in the literature a bewildering number of antibody labeling protocols. (novusbio.com)

ApoB antibody detects endogenous levels of total ApoB. (affbiotech.com)

APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E. The immunogen is located within amino acids 220 - 270 of APO-E. (thermofisher.com)
The custom peptide synthesis team uses our Immunogenicity Algorithm® to optimize and make a variety of antigens including those with modified amino acids such as phosphorylated residues, biotinylated residues, nitrosylated residues, and more. (aveslabs.com)

Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abcepta.com)

Antibodies: TACE (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. (qedbio.com)
Antibodies: TACE 1131 (0.25 ug/mL) and beta-actin 3779 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. (qedbio.com)
Antibodies: TACE 1131 (0.5 ug/mL), TACE 22-001 (2 ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. (qedbio.com)

we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented. (thermofisher.com)
We supply quality antibodies and related products drug discovery and academic laboratories worldwide. (abcepta.com)
animal products or animal by-products containing poultry or livestock derivatives (such as chicken, sheep or goat polyclonal antibodies) may require an Import Permit. (thenativeantigencompany.com)
The mAb samples before purification had.The coagglutination MK-2894 products could be observed with naked eyes in 5 min and verified the presence of GCA in the samples. (eprf.ca)
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. (scientificlabs.ie)
products, such as antigens and endotoxins, can protect against the development of allergy. (cdc.gov)

Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis. (cytekbio.com)
Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. (cytekbio.com)
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. (biolegend.com)

The successful implementation of vaccination programs in industrialized and many developing countries indicates that most of these populations have antibodies against the diphtheria toxin. (cdc.gov)

ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). (rndsystems.com)

Finding a compatible donor for kidney transplant candidates requires overcoming immunological barriers such as human leukocyte antigens (HLA) compatibility and ABO compatibility. (bvsalud.org)

Anatomy21

Organisms12

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment61

Phenomena and Processes45

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science3

Health Care4

Polyclonal antibody9

Chromatography6

Specificity8

Reactivity5

Monoclonal antibodies7

Proteins14

Conjugation2

Mouse monoclonal antibody1

ELISA3

Reagents4

Dilution12

Concentration4

Target antigen5

Conjugate3

Recombinant1

Peptides7

High-affinity4

Secondary11

Buffer4

Cell surface antigen2

Protein16

Rabbit1

Custom antibody5

Leukocyte common2

Immunogen for this antibody1

Biotin2

Isotype2

Reactive3

Endotoxin1

RRID1

Serum3

Reacts1

Compatible with 10x2

Conjugates2

Purity1

Immunoassays1

Prestige Antibody1

Cells18

Sequence antibodies1

Immunization1

Detection3

Titer1

Binds2

Immunofluorescence2

Specific antibody1

Primary3

Epitope1

Detect1

Protocols3

Detects1

Amino2

Stringent1

Incubation3

Products6

Flow cytometric analysis3

Diphtheria toxin1

Ligands1

Human1