• A peptide library is a tool for studying proteins. (wikipedia.org)
  • This form of peptide synthesis is limited to a peptide chain length of approximately 70 amino acids, and is generally unsuitable for the study of larger proteins. (wikipedia.org)
  • Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. (wikipedia.org)
  • The proteins that the phages are displaying can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those of other molecules. (wikipedia.org)
  • In this way, large libraries of proteins can be screened and amplified in a process called in vitro selection, which is analogous to natural selection. (wikipedia.org)
  • Peptides are short chains of amino acids that can bind to proteins and change their function. (epfl.ch)
  • First, binding affinity: Good binding requires difficult peptide architectures and amino acid sequences that are perfectly complementary in shape and charge to the surface of their target proteins. (epfl.ch)
  • ProteoMiner protein enrichment technology employs a large bead-based library of combinatorial peptide ligands, which dilutes high-abundance proteins and concentrates low-abundance proteins. (bio-rad.com)
  • When a complex biological sample is applied to the beads, high-abundance proteins saturate their high-affinity ligand(s) and excess protein is washed away. (bio-rad.com)
  • In contrast, low-abundance proteins are concentrated on their specific affinity ligand(s). (bio-rad.com)
  • The present invention envisions phosphorylated forms of any one of these GEF-H1 peptides, polypeptides and proteins. (justia.com)
  • Several covalent bonds, called peptide bonds , join amino acids together to form proteins . (peptidesguide.com)
  • A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
  • Designing synthetic proteins that can act as drugs for cancer or other diseases can be a tedious process: It generally involves creating a library of millions of proteins, then screening the library to find proteins that bind the correct target. (mit.edu)
  • That is the standard approach: Either completely randomly, or with some prior knowledge, design a library of proteins, and then go fishing in the library to pull out the most promising members," Keating says. (mit.edu)
  • The FOx BIOSYSTEMS instrument is effective in studying affinity and binding kinetics of proteins on large particles, such as cells, viruses and microvesicles. (foxbiosystems.com)
  • Antibodies can be engineered to target peptides derived from oncogenic proteins that are presented on the cell surface by human leukocyte antigens (pHLA). (bmj.com)
  • We use structure based modeling and computational protein design methods to optimize the protein sequence of proteins and peptides in order to control their assembly. (lu.se)
  • Small-molecule Peptide-Influenced Drug Repurposing (SPIDR) was developed to identify small molecule drugs that target a specific receptor by exploring the conformational binding space of peptide ligands. (biomedcentral.com)
  • The ability of ESI-MS to estimate simultaneously the relative binding affinities of a protein to soluble ligands in a library, if general, should be useful in drug development. (figshare.com)
  • This method identifies peptide ligands as well as cytoxic peptides that may prove useful in developing anti-cancer agents. (peptidesguide.com)
  • Libraries in and of themselves can prove even more uniform than other methods in demonstrating full-length ligands during screening since molecular weight degeneracy can be eliminated right during the sequencing process versus during the synthesis, thus making the entirety of the technique much more simplified and less costly. (peptidesguide.com)
  • Next, using a library of altered peptide ligands with different affinities, we perform time-lapse microscopy and functional analysis in the 3D system. (ox.ac.uk)
  • The cluster glycoside effect describes the remarkably high affinities of polyvalent saccharide ligands for polyvalent lectins, as measured by agglutination assays. (grantome.com)
  • 2. Develop combinatorial libraries of peptide-linked bivalent carbohydrate ligands for rapid screening of large numbers of compounds. (grantome.com)
  • 4. Study both the crystal and solution structures of high affinity ligands, to determine structural features of polyvalent ligands relevant to tight binding. (grantome.com)
  • 1998). In both examples, the high affinity ligands can compete with the intramolecular interactions and release the catalytic domains for their in vivo targets. (lu.se)
  • Boder, E. T. & Wittrup, K. D. Yeast surface display for screening combinatorial polypeptide libraries. (nature.com)
  • This thesis will document a method of combinatorial chemistry to construct libraries of new potential peptide-based imaging agents for positron emission tomography (PET) imaging. (uwo.ca)
  • Novel low-abundance allergens from mango via combinatorial peptide libraries treatment: A proteomics study. (ox.ac.uk)
  • By exploiting the combinatorial peptide ligand library (CPLL) technology, mango proteomes have been extracted and the presence of traces of allergens assessed via Western blot analysis two-dimensional maps. (ox.ac.uk)
  • Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage (long, thin viruses that infect bacteria) by fusing the virus's capsid protein to one peptide out of a collection of peptide sequences. (wikipedia.org)
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • Typically, nucleotide sequences encoding the protein/peptide library are fused to a gene encoding a phage coat protein thus allowing them to be displayed on the phage exterior. (cdc.gov)
  • Concept of bioinspired compatibilizer was expanded to the implementation of different peptide sequences. (hu-berlin.de)
  • Evaluation of secondary interactions revealed that the smaller the affinity for peptide-peptide interaction, the higher the availability of peptide sequences for an inorganic surface. (hu-berlin.de)
  • Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. (whiterose.ac.uk)
  • Thus, in one embodiment, peptides comprising the sequences described in SEQ ID NOs. (justia.com)
  • The new strategy involves first creating a computer model that can relate peptide sequences to their binding affinity for the target protein. (mit.edu)
  • The use of phage display libraries exploits the ability of MHC class II molecules to exchange peptides in solution and thus select out peptide sequences with high-affinity binding from a large array of random peptides. (ox.ac.uk)
  • SPIDR was tested using the potent and selective 16-amino acid peptide α -conotoxin MII ligand and the α 3 β 2 -nicotinic acetylcholine receptor (nAChR) isoform. (biomedcentral.com)
  • SPIDR incorporates a genetic algorithm-based, heuristic search procedure, which was used to explore the ligand binding domain of the α 3 β 2 -nAChR isoform using a library consisting of 640,000 α -conotoxin MII peptide analogs. (biomedcentral.com)
  • The αβ T-cell coreceptor CD4 enhances immune responses more than 1 million-fold in some assays, and yet the affinity of CD4 for its ligand, peptide-major histocompatibility class II (pMHC II) on antigen-presenting cells, is so weak that it was previously unquantifiable. (lu.se)
  • The affinity of CD4/pMHC II therefore seems to be set at a value that increases T-cell sensitivity by enhancing phosphorylation, without compromising ligand discrimination. (lu.se)
  • For example, a peptide chain of 10 residues in length is used in native chemical ligation with a larger recombinantly expressed protein. (wikipedia.org)
  • The peptide fills an alpha-BTX pocket made of residues located at fingers I and II, as well as at the C-terminal region. (rcsb.org)
  • Of the peptide residues, the largest contact area is formed by Tyr-3 and Tyr-4. (rcsb.org)
  • These findings are in accord with the previous data in which it had been shown that substitution of these aromatic residues by aliphatic amino acids leads to loss of binding of the modified peptide with alpha-BTX. (rcsb.org)
  • Further, a hexapeptide is a peptide that contains six amino acid residues. (peptidesguide.com)
  • Furthermore, we screened a single variant library of the V2 TCRm antibody to identify key residues for peptide specificity and affinity enhancement. (bmj.com)
  • Because ELR1-DE encodes a peptide of only 23 residues presumably, just ELR1-IN was analyzed further. (angiogenesis-blog.com)
  • Large random peptide libraries are often used for the synthesis of certain peptide molecules, such as ultra-large chemical libraries for the discovery of high-affinity peptide binders, and an increase in the library size severely affects parameters, such as the synthesis scale, the number of library members, the sequence deconvolution and peptide structure elucidation. (wikipedia.org)
  • Phage display is used to identify protein or peptide binders to a wide variety of targets. (cdc.gov)
  • An affinity based selection technique (biopanning) is used to identify binders from the library. (cdc.gov)
  • After screening the libraries, the researchers were able to isolate high-affinity binders to important protein targets. (epfl.ch)
  • De Novo Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. (u-tokyo.ac.jp)
  • Abstract: We have developed a massively parallel protocol for designing peptide binders. (tjbrunette.org)
  • Because the four cysteines can be placed in many different ways along the sequence of amino acids, the double-bridging strategy allowed the researchers to generate an enormously large number of structurally diverse peptide architectures. (epfl.ch)
  • The scientists also found that the four cysteines could be bridged by two linkers in three different ways, giving rise to three different architectures from each single peptide sequence. (epfl.ch)
  • The current concept is based on the sequence specific recognition of the inorganic surface by peptides. (hu-berlin.de)
  • Peptide-polyethylene oxide (PEO) conjugates, consisting of a previously identified specifically binding peptide sequence and polymer-block, are incorporated in the polymer composite material, composed of MgF2 nanoparticles particles and PEO. (hu-berlin.de)
  • A two-pool OBOC strategy was developed to distribute the fluorine-containing amino acid throughout the peptide sequence during library synthesis. (uwo.ca)
  • The invention also envisages a peptide consisting essentially of the sequence of SEQ ID NO. 3. (justia.com)
  • In another embodiment, a peptide consisting essentially of the sequence of SEQ ID NO. 4 is also provided. (justia.com)
  • In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
  • In another embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is less than 25 amino acids in length. (justia.com)
  • In a more preferred embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is 18 amino acids long. (justia.com)
  • This assists in knowing what is the most important within a sequence, thus avoiding the use of lengthy peptide chains. (peptidesguide.com)
  • Screening of a phage-display library demonstrated that two glycoprotein-specific mAbs recognized a conserved sequence, WxxxDI, which aligned between aa 14 and 19 of the mature glycoprotein. (microbiologyresearch.org)
  • Major histocompatibility complex (MHC) molecules bind peptides bearing an appropriate 'sequence motif' for MHC binding. (ox.ac.uk)
  • In 1990, Jamie Scott and George Smith described creation of large random peptide libraries displayed on filamentous phage. (wikipedia.org)
  • These precise epitope investigations were performed using two different phage-displayed random peptide libraries with different characteristics (T7 phage library gene X C9C and linear 9-mers, and M13 filamentous phage library gene III C7C and linear 12-mers and gene VIII 5C4C4). (nottingham.ac.uk)
  • A small number of H-bonds between peptide and antibody contribute to the affinity and specificity. (elsevierpure.com)
  • In total, 17 candidate peptides were found and their specificity toward Pb 2+ was further investigated with bead-based enzyme immunoassay (EIA). (korea.ac.kr)
  • Moreover, the affinity matured process yielded V2 variant antibodies with comparable or enhanced affinity, but none retained both specificity and increased T-cell activation. (bmj.com)
  • This displayed the different peptides on the outer surfaces of the collection of viral clones, where the screening step of the process isolated the peptides with the highest binding affinity. (wikipedia.org)
  • Compound identification was accomplished by searching against a locally-established mass spectral library of 174 fentanyl analogs and metabolites. (cdc.gov)
  • In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively. (wikipedia.org)
  • In this work, we have developed antibodies to NPs from a phage library containing approximately 2x109 unique single-chain variable fragment (scFv) antibodies each displayed monovalently on the gene III coat protein of a M13 filamentous phage. (cdc.gov)
  • The bacteriophage M13 is a filamentous phage that displays peptides linked to the minor coat protein III gene. (springeropen.com)
  • Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD surface functionalisation by antibodies. (whiterose.ac.uk)
  • The three-dimensional structure of 2H1, a protective monoclonal antibody to Cryptococcus neoformans, has been solved at 2.4 Å resolution, in both its unbound form and in complex with the 12 amino acid residue peptide PA1 (GLQYTPSWMLVG). (elsevierpure.com)
  • Peptide binding is associated with only minor rearrangements of some side-chains and a small shift in the H2 loop of the antibody. (elsevierpure.com)
  • The peptide assumes a tightly coiled conformation consisting of one inverse γ-turn and one type II β-turn that serves to place the entire peptide motif, consisting of Thr(P5) Pro(P6), Trp(P8) Met(P9) and Leu(P10), into a depression in the antibody combining site. (elsevierpure.com)
  • Poor steric complementarity between PA1 and the antibody heavy chain along with the fact that the majority of the interactions between 2H1 and PA1 involve van der Waals interactions with the light chain may explain why this peptide acts as only a partial mimotope of the capsular polysaccharide epitope. (elsevierpure.com)
  • Results The cryoEM complex structure revealed the V2 TCRm antibody sits on top of the KRAS G12V -HLA-A*03:01 binding groove, making multiple contacts and leaning heavily towards the C-terminus of the KRAS G12V peptide. (bmj.com)
  • All contacts made between the V2 TCRm antibody and KRAS G12V peptide were aliphatic and hydrophobic in nature, with no hydrogen bonds made directly with the peptide. (bmj.com)
  • Structural alignment of the V2 bound KRAS G12V -HLA-A*03:01 and unbound KRAS WT -HLA-A*03:01 structures revealed the KRAS G12V peptide underwent a conformational change upon antibody binding. (bmj.com)
  • Conclusions This complex structure is the first cryoEM structure of an antibody fragment binding a neoantigen-HLA target and the first structures of the KRAS WT/G12V peptides presented by HLA-A*03:01 with or without an antibody in complex. (bmj.com)
  • Characterizing how the V2 TCRm antibody recognizes KRAS G12V -HLA-A*03:01 and differences in KRAS WT and KRAS G12V peptide binding to HLA-A*03:01 offers insight into how highly hydrophobic peptide neoantigens can be targeted with antibody-based therapies. (bmj.com)
  • H-T Huang, X-Y Dong, Y Sun, Q-H Shi*: Biomimetic affinity chromatography for antibody purification: Host cell protein binding and impurity removal. (tju.edu.cn)
  • Endopep-MS currently uses an antibody-affinity concentration/purification step before the enzymatic reaction with the substrate, and the choice of antibody is critical for the success of this assay [ 5 ]. (cdc.gov)
  • The bound peptide adopts an almost-globular conformation resulting from three turns that surround a hydrophobic core formed by Tyr-11 of the peptide. (rcsb.org)
  • At least five subtypes of these libraries exist, and the distinguishing characteristic is the method of synthesis of each library. (wikipedia.org)
  • Chapter 2 discusses the synthesis of a library of N-terminal modified peptides created using a one-bead one-compound (OBOC) approach. (uwo.ca)
  • Chapter 4 discusses the synthesis of an OBOC library with an imaging moiety integrated within the peptide structures. (uwo.ca)
  • However, since there are 20 natural amino acids, the synthesis of a linear peptide ( n amino acids in length) can be produced in 20 n different combinations. (peptidesguide.com)
  • The first specific aim focuses on the synthesis and characterization of peptide amphiphiles that can form nanostructures in water. (grantome.com)
  • Based on similarity, infiniSee finds molecules of interest in screening libraries or Chemical Spaces of almost infinite size. (biosolveit.de)
  • We have analysed the peptide binding motifs of HLA-DRB1*1301 and *1302 using affinity purified HLA-DR13 molecules to purify sequentially HLA-DR13-binding peptides from a large random library of M13 phage containing nonamer inserts in the pIII coat protein. (ox.ac.uk)
  • Whereas binding was undetectable in solution, the affinity of the CD4/pMHC II interaction could be measured in 2D using CD4- and adhesion molecule-functionalized, supported lipid bilayers, yielding a 2 D Kd of ∼5,000 molecules/μm 2 . (lu.se)
  • Thus, the overall motif or GAD-6 may correspond to 523-528 aa of GAD-65 (M-S-R-L-S-K). To further define the GAD-6 epitope, sequencing or GAD-6 reactive peptides which were obtained from the successful biopanning using MI3 gene III C7C and linear 12-mers phage libraries, did not show a clear motif and did not show reactivity with GAD-6 by capture ELISA. (nottingham.ac.uk)
  • A possible explanation for this is that the peptides which are specific to GAD-6 are not present in these M13 pIII phage libraries. (nottingham.ac.uk)
  • Thus, it is possible to develop a library of 20 different polypeptides along with just one amino acid residue randomly, with all the rest being the same. (peptidesguide.com)
  • The analysis of the human high affinity IgE receptor Fc epsilon Ri alpha from multiple crystal forms. (expasy.org)
  • The structure of the complex may explain the fact that the library-derived peptide binds alpha-BTX with a 15-fold higher affinity than that shown by the acetylcholine receptor peptide (alpha185-196). (rcsb.org)
  • The result is two novel peptide‐based PET imaging agents that possess high affinities for their targets, the growth hormone secretagogue receptor 1a (GHSR‐1a) and gastrin‐releasing peptide receptor (GRPR), with IC 50 values of 9.7 and 0.50 nM, respectively. (uwo.ca)
  • Prearranged positive allosteric modulators (PAMs) inspired by photopharmacology are synthesized and applied to the glucagon-like peptide-1 receptor (GLP-1R), a class B G protein-coupled receptor. (wiley.com)
  • By producing large numbers of peptides, for which each differs from the other by one single amino acid, it is possible to ascertain which amino acid substitutions make binding to a receptor the strongest, and which make that binding process weaker. (peptidesguide.com)
  • Engineering of TCRs requires stabilization of the receptor prior to engineering for higher affinity. (illinois.edu)
  • loop_ _audit_author.name _audit_author.pdbx_ordinal 'Harel, M.' 1 'Kasher, R.' 2 'Sussman, J.L.' 3 # _citation.id primary _citation.title ;The Binding Site of Acetylcholine Receptor as Visualized in the X-Ray Structure of a Complex between Alpha-Bungarotoxin and a Mimotope Peptide. (rcsb.org)
  • Endomorphin (EM)-1 and EM-2 are tetrapeptides with high affinity and selectivity for the micro-opioid receptor. (omeka.net)
  • In the field of bacteriophage affinity peptides, it is becoming more popular to use the entire phage as a receptor rather than the individual peptides. (foxbiosystems.com)
  • Calculations indicated, however, that CD4/pMHC II binding would increase rates of T-cell receptor (TCR) complex phosphorylation by threefold via the recruitment of Lck, with only a small, 2-20% increase in the effective affinity of the TCR for pMHC II. (lu.se)
  • They may also interact synergistically with plexins and with VEGF RECEPTORS to form receptor complexes with distinct affinities and specificities. (bvsalud.org)
  • Each peptide in the library contains a single fluorine atom incorporated through established SPAAC chemistry. (uwo.ca)
  • This 18 F-azadibenzocyclooctyne (ADIBO-F) was radiolabelled from its toluenesulfonate precursor in 21-35 % radiochemical yields, and subsequently incorporated into two cancer-targeting peptides through SPAAC chemistry. (uwo.ca)
  • from the technical review on the medicinal chemistry and molecular pharmacology of opioid peptides and the opiates which took place on September 4-6, 1984, at Bethesda, Maryland"--Page iv. (epa.gov)
  • The unique screening technique as described above uses peptide chemistry along with the OBOC library format. (peptidesguide.com)
  • Phage display technology has been used as a powerful tool in the discovery of peptides capable of exhibiting specific affinity to various metals or metal ions. (korea.ac.kr)
  • Cyclization of peptides with two chemical bridges affords large scaffold diversities. (epfl.ch)
  • Synthetizing methods for developing one-bead, one-compound (OBOC) peptide libraries have shown to be superior to that of standard assay formats and pooling strategies. (peptidesguide.com)
  • In an off-bead assay, the peptide is released from the resin bead, but it maintains its proximity to the bead. (peptidesguide.com)
  • To further define the N-terminal MoAb epitope, sequencing of N-terminal MoAbs reactive peptides which were obtained from the successful biopanning using M13 gene VIII 5C4C4 phage Iibrary and were selected by high affinity binding in immnuo-blotting assay using nitro-cellulose membrane and in capture ELISA. (nottingham.ac.uk)
  • This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
  • The peptides' highly efficient binding, combined with their small size and high stability make them ideal for drug therapies. (epfl.ch)
  • They show high binding affinity, low toxicity, and are easy to synthesize, all of which makes peptides ideal for use in drug development, and many naturally occurring peptides such as insulin, oxytocin, somatostatin and the antibiotics vancomycin or polymyxin B, are already successfully used like that. (epfl.ch)
  • Wilson, D. S., Keefe, A. D. & Szostak, J. W. The use of mRNA display to select high-affinity protein-binding peptides. (nature.com)
  • Allocation of the library and a test cell line within a soft agar matrix followed by a partial peptide release will result in high concentrations of peptide in the agar surrounding each bead. (peptidesguide.com)
  • Chapter 4 details the engineering of a high affinity TCR in its native state, without the need for stabilization, using a T cell display system. (illinois.edu)
  • The group returned to their results from the original phage display library, where a second peptide with high binding affinity to the midgut lumen, named MP2 , had been identified. (biomedcentral.com)
  • It is expected that this monolith-based chromatographic biopanning will provide a promising approach for a high throughput screening of affinity peptides cognitive of a wide range of target species. (korea.ac.kr)
  • The ureido ring is involved in the high affinity binding of biotin to avidin, a glycoprotein found in egg-white. (medscape.com)
  • Second, peptide stability: peptides can potentially be cut by enzymes (proteases) into smaller and useless fragments or even single amino acids. (epfl.ch)
  • Stability was also a highly attractive feature of the new peptide format. (epfl.ch)
  • EPFL scientists have developed a new type of "double-bridged peptide" that can be tailored to bind tightly to disease targets of interest. (epfl.ch)
  • Using the double-bridge approach for both these protein targets, the scientists developed peptides that could bind them efficiently at nanomolar concentrations. (epfl.ch)
  • Based on the results, Heinis's lab is now applying the peptide format to many other disease targets. (epfl.ch)
  • They have already developed new, even larger double-bridged peptide libraries and screened them against a range of disease-relevant targets. (epfl.ch)
  • In this Perspective, we discuss the recent progress in using DNA-encoded chemical libraries to interrogate complex biological targets and their potential to identify structures that elicit function or possess other useful properties. (nature.com)
  • Analysis of peptide-binding motifs for two disease associated HLA-DR13 alleles using an M13 phage display library. (ox.ac.uk)
  • The solution structure of the complex between alpha-bungarotoxin (alpha-BTX) and a 13-residue library-derived peptide (MRYYESSLKSYPD) has been solved using two-dimensional proton-NMR spectroscopy. (rcsb.org)
  • Here, we report that a soluble form of CD4 failed to bind detectably to pMHC II in surface plasmon resonance-based assays, establishing a new upper limit for the solution affinity at 2.5 mM. (lu.se)
  • Glu-5 and Leu-8, which also remarkably contribute to the contact area with the toxin, are present in all the library-derived peptides that bind strongly to alpha-BTX. (rcsb.org)
  • We start by docking the peptide onto a curated scaffold library generated from chopped up repeat protein junctions that range from 80-121aa. (tjbrunette.org)
  • During which, we also make sure not to leave any charge groups buried, and make sure there is a strong hydrogen bonding network between the scaffold and the peptide. (tjbrunette.org)
  • A Rosetta design protocol that minimizes the number of unsatisfied hydrogen bonds on the peptide. (tjbrunette.org)
  • The peptides that exhibited the highest affinity for α 3 β 2 -nAChR were used as models for a small-molecule structure similarity search of the PubChem Compound database. (biomedcentral.com)
  • Once docked, we optimize (redesign) the interface to maximize affinity and selectivity. (tjbrunette.org)
  • Additionally, the antibodies have been characterized for their binding and cross-reactivity properties to several other NPs, and some challenges associated with the isolation of the antibodies from a large library and alternative method for selection of antibodies have been discussed. (cdc.gov)
  • DNA-encoded chemical libraries (DELs) have emerged as a powerful technology in drug discovery. (nature.com)
  • The library of complete imaging agents was put through multiple stages of screening against CXCR4, resulting in the discovery of a peptide-based imaging agent with an IC 50 of 138 µM. (uwo.ca)
  • An on-bead two-colour screen was performed in one step and resulted in the discovery of three CXCR4-specific peptide-based imaging agents with micromolar affinities of 28, 86 and 109 µM. (uwo.ca)
  • OBOC libraries are very powerful tools that are utilized for the discovery of cysteine-rich peptides for protein tagging as well as cysteine-rich pharmaceuticals, and more. (peptidesguide.com)
  • Toxicity, binding affinity and cell internalization of a library of nanostructures containing the Claudin-1 targeting peptide will be assessed. (grantome.com)
  • This peptide library would be useful for analyzing the effect of the post-translational modification acetylation on lysine which neutralizes the positive charge. (wikipedia.org)
  • This bioinspired concept of interface modification via peptide-polymer conjugates represents a versatile tool for new material development. (hu-berlin.de)
  • In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. (whiterose.ac.uk)
  • Polymyxin resistance can develop after modification of the lipid A component in the lipopolysaccharide structure through mechanisms that are chromosomally mediated and result in a reduction in the affinity for these cationic peptides ( 5 , 6 ). (cdc.gov)
  • Synthetic peptide libraries are synthesized without utilizing phage or other biological systems. (wikipedia.org)
  • A 12 aa synthetic peptide of this epitope was recognized by both mAbs and sera from a subset of rabies-vaccinated dogs. (microbiologyresearch.org)
  • Usually, the peptide library is synthesized on a solid phase, mostly on resin, which can be made as a flat surface or beads. (wikipedia.org)
  • The atomic interactions between subunits in protein assemblies are finely tuned to control affinity as well as quaternary structure. (lu.se)
  • Elution can be done combining low-pH elution buffer with sonification, which, in addition to loosening the peptide-target interaction, also serves to detach the target molecule from the immobilization surface. (wikipedia.org)
  • PA1 was previously identified as a potential mimotope of the cryptococcal capsular polysaccharide by screening of a phage display peptide library. (elsevierpure.com)
  • Here huge numbers of peptides are screened so as to isolate and identify critical bioactive peptides that are used for new ways to cure diseases and cancers. (peptidesguide.com)
  • Computational tools to modify the claudin-1 targeting peptide will be used to guide optimization of nanostructure targeting. (grantome.com)
  • Branched Multimeric Peptides as Affinity Reagents for the Detection of alpha-Klotho Protein. (u-tokyo.ac.jp)
  • The presence of a particular BoNT serotype is demonstrated by mass spectrometric detection of the peptide cleavage products corresponding to their specific toxin-dependent location. (cdc.gov)
  • Currently, our library can identify 150 fentanyl-related compounds from the Fentanyl Analog Screening (FAS) Kit), plus an additional 25 fentanyl-related compounds from individual purchases. (cdc.gov)
  • Peptide libraries typically contain a large number of peptides that have a systematic combination of amino acids. (wikipedia.org)
  • The "bottom-up" approach bypasses the limitations of enumerated libraries through identification of the essential binding units, the fragments, and the subsequent selection of compounds containing those substructures from large chemical spaces. (biosolveit.de)