• The fact that the PBD region of PAK has a high affinity for both GTP-Rac and GTP-Cdc42 and that PAK binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of both Rac and Cdc42 make it an ideal tool for affinity purification of GTP-Rac and GTP-Cdc42 from cell lysates. (cytoskeleton.com)
  • This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. (cytoskeleton.com)
  • loop_ _audit_author.name _audit_author.pdbx_ordinal 'Kroker, A.J.' 1 'Bruning, J.B.' 2 # _citation.id primary _citation.title 'p21 Exploits Residue Tyr151 as a Tether for High-Affinity PCNA Binding. (rcsb.org)
  • Here we report that two compounds, FT671 and FT827, inhibit USP7 with high affinity and specificity in vitro and within human cells. (uel.ac.uk)
  • FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. (thermofisher.com)
  • When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. (thermofisher.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotensin Receptor 1, High Affinity (NTSR1) in Tissue homogenates, cell lysates and other biological fluids. (1elisakits.com)
  • Description: A sandwich ELISA kit for detection of Neurotensin Receptor 1, High Affinity from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (1elisakits.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Neurotensin Receptor 1, High Affinity (NTSR1) in samples from tissue homogenates or other biological fluids. (1elisakits.com)
  • Specific high affinity binding proteins for THYROID HORMONES in target cells. (bvsalud.org)
  • This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. (abcam.cn)
  • The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.com)
  • The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). (affbiotech.cn)
  • The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. (cytoskeleton.com)
  • HPIMBD considerably induces proteins expression degrees of tumor suppressors p53 and p21 in MCF-7 cells. (brain-tumor-cancer-information.com)
  • The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). (rockland.com)
  • In addition acetylation of transcription factors offers been shown elsewhere to impact the stability and intracellular localization of proteins or to modulate the affinity for DNA or additional proteins (34). (bio2009.org)
  • Proliferating cell nuclear antigen (PCNA) plays an essential role in DNA replication, repair, and control of cell proliferation, and its activity can be modulated by interaction with p21(Wafl/Cip1) [Cox, L. S., (1997) Trends Cell Biol. (dundee.ac.uk)
  • Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 µM). (sigmaaldrich.com)
  • The assay uses the Cdc42/Rac Interactive Binding (CRIB) region (also called the p21 Binding Domain, PBD) of the Cdc42 / Rac effector protein, p21 activated kinase 1 (PAK). (cytoskeleton.com)
  • p21 has been recognized by virtue of its activation by p53 (13) its association with cyclin/cyclin-dependent kinase (CDK) complexes (23 66 and its up-regulation during senescence (47). (bio2009.org)
  • The adaptor protein downstream of tyrosine kinase 2 (Dok2) bound directly to the phosphorylated NPLY motif with a 10-fold higher affinity (K(D) of approximately 1 microM at 37 degrees C) than the closely related Dok1. (ox.ac.uk)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • The affinity constant for the interaction between p21 (141-160) peptide and PCNA has been determined to be 1.14 x 10 7 M -1 , corresponding to a Kd of 87.7 nM. (dundee.ac.uk)
  • Measurement of the interaction of truncation and substitution analogues based on the p21 (141-160) sequence with PCNA revealed that the N-terminal part (residues 141-152) of the above peptide is the minimum recognition motif, required for PCNA binding. (dundee.ac.uk)
  • Consensus motif 1 peptide and p21 (141-160) have similar affinities for binding PCNA and abilities to inhibit in vitro replication of DNA originated from SV40. (dundee.ac.uk)
  • Purification: This antibody is purified through a protein A column, followed by peptide affinity purification. (hatinhibitor.com)
  • Dok2 was phosphorylated in response to CD200R engagement and recruited RAS p21 protein activator 1 (RasGAP). (ox.ac.uk)
  • We further demonstrate that HPIMBD significantly inhibits protein expression levels of oncogenes c-Myc and cyclin D1 and induces protein expression levels of tumor suppressors p53 and p21 in MCF-7 breast cancer cell line. (brain-tumor-cancer-information.com)
  • The tumor suppressor p53 directly interacts with Sp1 and may compete with the transcriptional repressor HDAC1 for binding to the C terminus of Sp1 leading to histone acetylation and concomitant manifestation of p21. (bio2009.org)
  • p21 and other tumor suppressor genes' inactivation are therefore most likely involved in the first steps of the cancer model, with later stages possibly involving protooncogene activation and inflammation. (medscape.com)
  • We also show that ARF promotes p53 mutant stability in tumors and suppresses p73 mediated p21 expression and senescence. (oncotarget.com)
  • In this study, the formation of complexes between PCNA and peptides derived from the C-terminus of p21 has been investigated at the molecular level and quantified using a competitive PCNA binding assay and isothermal titration calorimetry (ITC). (dundee.ac.uk)
  • Truncation of the C-terminal region p21 (153160), though, inhibited significantly the ability of the peptides to compete with the full-length p21 (141160) for binding to PCNA. (dundee.ac.uk)
  • Using this assay, we have screened rationally designed peptides for binding to PCNA and interruption of the PCNA-p21 (141-160) complex. (dundee.ac.uk)
  • Furthermore the p21 protein was shown previously to interact 67346-49-0 with the proliferating cell nuclear antigen (PCNA) thereby preventing DNA replication (10). (bio2009.org)
  • Cite Format: Affinity Biosciences Cat# AF3036, RRID:AB_2834325. (affbiotech.com)
  • 引用格式: Affinity Biosciences Cat# AF0877, RRID:AB_2834413. (affbiotech.cn)
  • The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Rac (or GTP-Cdc42) complex with glutathione affinity beads. (cytoskeleton.com)
  • p21 interacts with cyclin E. (uth.edu)
  • Recently several reports showed the induction of p21 by inhibitors of histone deacetylases (HDACs) such as for example sodium butyrate (46) trichostatin A (TSA) (56) suberoylanilide hydroxamic acidity (51) oxamflatin (32) MS-27-275 (52) apicidin (22) and trapoxin (54). (bio2009.org)
  • The transcriptional activation from CACNA1F the p21 gene by these inhibitors is normally marketed by chromatin redecorating pursuing acetylation of histones H3 and H4 in the p21 promoter area (32 54 This activation of p21 takes place within a p53-unbiased fashion and for that reason HDAC inhibitors are appealing realtors for cancers therapy being that they are operative in cells with mutated p53 genes a hallmark of several tumors. (bio2009.org)
  • effects the main target for p53-induced cell cycle arrest seems to be the p21 gene. (bio2009.org)
  • Likewise the p21 67346-49-0 gene could be turned on by transforming development aspect β Ca2+ lovastatin or nerve development aspect (16). (bio2009.org)
  • The promoter from the individual p21 gene harbors six conserved GC containers binding sites for the transcription aspect Sp1. (bio2009.org)
  • With this statement we display that HDAC1 is definitely a crucial regulator of p21 gene manifestation. (bio2009.org)
  • Activation of p53 efficiently counteracts deacetylase-mediated repression and induces cell cycle arrest by activating the p21 gene. (bio2009.org)
  • This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. (thermofisher.com)
  • An in vitro binding kinetic analysis revealed that these phosphorylation-dependent events were attributable to a decrease in the affinity of phosphorylated Homer3 for its ligand. (jneurosci.org)
  • Induction of p21 expression by genotoxic stress and its role during terminal differentiation of various cell types have been investigated intensively. (bio2009.org)
  • Consistent with USP7 target engagement in cells, FT671 destabilizes USP7 substrates including MDM2, increases levels of p53, and results in the transcription of p53 target genes, induction of the tumour suppressor p21, and inhibition of tumour growth in mice. (uel.ac.uk)
  • Luciferase reporter constructs driven by the human being p21 promoter were previously. (bio2009.org)
  • mt) mice in comparison with outrageous type (wt) at p21. (woofahs.com)
  • These data supply the initial direct proof an IFT particle within a mammalian program and show the utility from the affinity-purified antibodies to IFT protein. (woofahs.com)
  • As a large international collaboration relying on similar technologies, the project has a natural affinity with high-energy physics. (press.cern)
  • While p21 is usually activated by p53-dependent mechanisms in response to DNA damage to make sure cell cycle arrest and repair a number of realtors that promote differentiation like phorbol ester 67346-49-0 or okadaic acidity can up-regulate p21 separately of p53 (for an assessment see reference point 16). (bio2009.org)
  • Usually, it is in the α-form, which has a monoclinic crystal structure with space group P21/n. (wikipedia.org)