Affinity methods peptide nucleic acids. Medical search. Frequent questions

This mini-review focuses on covalently linked conjugates of PNA with cell-penetrating peptides, aminosugars, aminoglycoside antibiotics, and non-peptidic molecules that were tested, primarily as PNA carriers, in antibacterial and antiviral applications. (bvsalud.org)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
Initiation and regulation of immune responses in humans involves recognition of peptides presented by human leukocyte antigen class II (HLA-II) molecules. (biomedcentral.com)
Experimental screening methods are expensive and time consuming and reagents are not readily available for many of the HLA class II molecules. (biomedcentral.com)
Full overlapping studies of four antigens identified binding affinity of 103 peptides to seven common HLA-DR molecules (DRB1*0101, 0301, 0401, 0701, 1101, 1301, and 1501). (biomedcentral.com)
CTLs recognize peptide antigens presented by major histocompatibility complex class I (MHC-I) molecules on infected cells or cancer cells and kill them. (biomedcentral.com)
Both these processes are initiated and regulated by T-helper (Th) cells that recognize antigenic peptides presented by MHC class II (MHC-II) molecules. (biomedcentral.com)
MHC-II molecules present antigenic peptides internalized by professional antigen presenting cells, such as macrophages, dendritic cells, or T lymphocytes. (biomedcentral.com)
Peptides presented by MHC-I molecules are mainly intracellular and those presented by MHC-II molecules originate mainly from or extracellular proteins. (biomedcentral.com)
A distinct characteristic of MHC molecules of either class is a groove that binds peptides in a highly promiscuous manner. (biomedcentral.com)
Biotinylation is the process of covalently attaching biotin to molecules (such as amino acids, peptides, antibodies, or proteins). (newyorktimesnow.com)
The approaches and results presented here will serve as a guide to systematically approach targeted delivery of cytotoxic drug molecules using peptides for treatment of several diseases. (biosyn.com)
Arg-Gly-Asp (RGD) peptides,2 poly-Arg peptides), 3,4 proteins (e.g., antibodies,5 transport proteins, and transferrin6), and small molecules (e.g., folate7) have been used to selectively direct drugs to cancer cells with upregulated receptors by forming drug-carrier conjugates (Fig. 1). (biosyn.com)
One example of this method is to produce a plurality modified antibodies by producing nucleic acids molecules that encode modified forms or light chains of the first antibody. (patentpc.com)
Central to the continued development of this field is the pursuit of new strategies and methods for the efficient construction of molecules. (northwestern.edu)
An assortment of affinity purification resins for the separation of both tagged and immunoglobulin molecules. (gbiosciences.com)
A large selection of affinity coupling chemistries are available to isolate your target molecules including carboxyl containing molecules, nucleic acids, glycoproteins, polyclonal antibodies, oxidized sugars and many more. (gbiosciences.com)
MHC class II molecules are specialized peptide receptors that play a critical role in initiating and regulating immune responses by binding peptide fragments that are 10-30 amino acids long [ 1 ] and present them on the surface of antigen-presenting cells for recognition by CD4 + T cells. (biomedcentral.com)
The use of computational techniques has been instrumental in advancing epitope-based vaccine research, with much work focusing on predicting the binding specificities of peptides to MHC molecules. (biomedcentral.com)
Sequence-based predictive systems, based on identifying patterns in peptides with experimentally determined binding strength, are widely used to facilitate the identification of binding peptides to MHC class II molecules. (biomedcentral.com)
Aptamers, short single-stranded nucleic acid or peptide molecules, can specifically bind to a wide range of targets, including proteins, small molecules, and even cells. (neoaptamers.com)
Aptamers are short, synthetic oligonucleotide or peptide sequences that adopt specific three-dimensional structures, allowing them to bind with high affinity and specificity to their target molecules. (neoaptamers.com)
Traditional aptamer selection methods have faced limitations in achieving commercial success as therapeutic agents, primarily due to the challenge of fully accounting for weak binding to off-target molecules. (neoaptamers.com)

The modifications introduced in affibodies, representing a class of small, three-helix engineered scaffold proteins, and antibodies were selected and implemented through rational design, using a combination of solid phase peptide synthesis, genetic engineering and enzymatic conjugation, depending on the case. (kth.se)
The affinity matured antibodies are more or less binding to a target antibody. (patentpc.com)
This article aims to offer methods for optimizing and enhancing the binding affiniites and selecting antibodies with desired affinity. (patentpc.com)
This document describes methods for affinity maturation of antibodies and fragments of them based on structure/activity relation (SAR). (patentpc.com)
These methods allow for the optimization of antibodies that have higher and better activity (e.g. (patentpc.com)
The method allows the comparison of the first antibody to 1, 2, 3, 4 or 5 related antibodies. (patentpc.com)
Recombinant proteins are utilized for various purposes in molecular biology such as the production of antibodies for investigation of the mechanism of protein-nucleic acid or protein-protein interactions. (dianova.com)
1995). Diisocyanates and acid anhydrides cyanates has a low association with atopy, low prevalence are among the most common low molecular weight (LMW) with specific IgE antibodies and a mixed Th1-Th2 response classes of chemicals responsible for occupational asthma. (cdc.gov)

Peptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. (bvsalud.org)

ORF1p binds single-stranded nucleic acid (ssNA) and exhibits NA chaperone activity. (nsf.gov)

Peptide nucleic acids are synthetic oligonucleotides that resist protease degradation and are used to induce repair at site specific triplex formation regions on DNA genomic sites. (wikipedia.org)

Membrane bioseparation has been widely used as an effective and reliable method for the separation and purification of bioproducts such as enzymes, proteins, peptides, and nucleic acids. (mdpi.com)
Different surface coatings and chemical properties give each type of bead its own binding properties, which can be used for the magnetic separation (separation and purification) of nucleic acids, proteins or other biomolecules in an easy, effective and scalable manner. (aisenbio.com)
As evidenced by the 1990 US patent, their potential in nucleic acid purification has been recognized. (aisenbio.com)
It also shows obvious advantages in nucleic acid probe labeling, separation and purification of cells and biologically active substances. (newyorktimesnow.com)
Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. (gbiosciences.com)
Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. (gbiosciences.com)
Many prokaryotic expression vectors have been established enabling synthesis of the protein of interest as a fusion with a peptide, thus facilitating purification. (dianova.com)
The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbant. (dianova.com)
Application Flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to gram levels of target product. (carnegiescience.edu)

Methods and reagents. (genomicglossaries.com)
Affinity proteins are important reagents in research, diagnostics and therapeutic settings. (kth.se)
The focus of this thesis has been on investigating different chemical and enzymatic strategies for engineering of affinity proteins to generate affinity reagents with improved or changed functionality. (kth.se)
The rapid discovery of selective affinity reagents to native proteins is a challenge in chemical biology and medicine. (aps2022.org)
The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of bifunctional boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. (justia.com)
This method minimizes the shearing force on the target molecule, requires fewer steps and reagents than other DNA extraction schemes, and is suitable for 24, 96 and 384-well plates. (aisenbio.com)
High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin . (gbiosciences.com)

Docking results of the multi-epitope vaccine peptide with human leukocyte antigen class I and II alleles predicted efficient binding and the resulted docked models were stable during simulation. (ijpsonline.com)

The peptide-binding groove of a MHC molecule consists of a β-sheet and two α-helices. (biomedcentral.com)
Peptides derived from sequence of cell surface proteins, such as intercellular adhesion molecule-1 (ICAM-1), LHRH, Bombesin, and LFA-1, have shown potent binding affinity to the target cell surface receptors. (biosyn.com)
germline segments for the nucleic acids molecule encoding variable light chain of first antibody. (patentpc.com)
Each nucleic Acid molecule contains one codon that codes an amino acid in the target area that encodes a different type of variable heavy chain. (patentpc.com)

They also overcame the cytosolic delivery barrier with cell-penetrating peptides, chemical vectors that facilitate cellular uptake and nuclear targeting of antisense cargo. (aps2022.org)

Using these reactive groups, biotin can be easily attached to most proteins and peptides. (newyorktimesnow.com)

A sophisticated high-order framework nucleic acid (FNA) was engineered for the targeted delivery and responsive release of environment tolerant antisense peptide nucleic acids (asPNAs). (bvsalud.org)
OBJECTIVES: To examine the bactericidal effect of antisense peptide-peptide nucleic acids (P-PNAs) that can block the expression of DNA gyrase or topoisomerase IV in FQ-resistant Escherichia coli (FRE). (bvsalud.org)
METHODS: A set of antisense P-PNA conjugates with a bacterial penetration peptide were designed to inhibit the expression of gyrA and parC and were evaluated for their antibacterial activities. (bvsalud.org)
Recently, the group retargeted the anthrax protective antigen to receptors overexpressed on tumor cells and modified its lethal factor component to target cancer gene dependencies with antisense peptide nucleic acids. (aps2022.org)
Harnessing the power of machine learning, best-in-class variants were predicted and designed de novo, outperforming all previously known peptides for antisense delivery, while being non-toxic and effective in mice. (aps2022.org)

Successful recognition of antigenic peptides bound to Major Histocompatibility Complexes (pMHCs) requires specific binding of the TCR to these complexes ( 5 - 7 ), which in turn modulates the cell's fitness, clonal expansion, and acquisition of effector properties ( 7 ). (frontiersin.org)

In molecular biology, magnetic beads provide a simple and reliable method to purify various types of biomolecules, including genomic DNA, plasmids, mitochondrial DNA, RNA and proteins. (aisenbio.com)
The main consideration in the construction of genomic or cDNA libraries is, therefore, the nucleic acid starting material. (biologydiscussion.com)

Nucleic Acids Res.16,3347-8, 1995. (dianova.com)

The conformational change induced by isomerization was hypothesized to be large enough to cause a loss in binding affinity in the conjugated affibody, which was tested in an affinity chromatography assay in which one of the affibodies captured to an IgG-sepharose column showed loss of affinity during illumination. (kth.se)
and HEV-C proteins were expressed in Escherichia coli and purified by using the Ni- nitrilotriacetic acid affinity chromatography assay (Qiagen) according to manufacturer instructions. (cdc.gov)

From March-August 2020, we rapidly deployed our methods to engineer ACE2-based receptor traps that potently neutralize live SARS-CoV-2 virus in cell culture as therapeutic candidates for further development (Glasgow et al. (ucsf.edu)

Peptide nucleic acid (PNA) probes have previously successfully been used for selective hybridization between the primary, tumor-targeting agent and the secondary agent in a pretargeting set-up for in vivo tumor imaging or directed therapy. (kth.se)
in vivo detection of protein-peptide interactions, high sensitivity, the capacity for rapid genetic screening to identify stronger and weaker binding peptide variants, and the use of a simple assay (transcriptional activity) as a means to assess binding affinity. (dartmouth.edu)

Dynamic and flexible nucleic acid models can provide current and future scientists with physical intuition for the structure of DNA and the ways that DNA and its synthetic mimics can be used to build self-assembling structures and advanced nanomachines. (allenpress.com)
Peptide nucleic acids (PNAs) are synthesized by attaching modified peptide backbones generally derived from repetitive N-2-aminoethyl glycine units in place of the regular phosphodiester backbone and represent synthetic impersonator of nucleic acids that offers an exciting research field due to their fascinating spectrum of biotechnological, diagnostic and potential therapeutic applications. (bvsalud.org)

Therefore, by synthesizing the N gene sequence of SARS-CoV-2, constructing the pET-28a (+)-N recombinant plasmid, we expressed the N protein in E.coli and obtained 15 mAbs against SARS-CoV-2-N protein by the hybridomas and ascites, then an immunochromatographic test strip method detecting N antigen was established. (pimac.org)
The activity to be assessed in the present method can include binding, signal transduction and differentiation, alteration or gene expression, cell proliferation, chemotaxis (cytotoxicity), cancer cell invasion, endothelial proliferation or tube formation. (patentpc.com)
CTCGAGCATCGGCACTGCAGCCGAG-3) were used to amplify the gene encoding the 239- aa (aa) recombinant peptide corresponding to aa 368-606 of HEV-C ORF2. (cdc.gov)
CTCGAGAGCAGAGTGGGGTGCTAAAACAC-3) were used to amplify the HEV-A gene encoding the recombinant peptide corresponding to aa 413-651 of HEV-A ORF2 (genotype 4). (cdc.gov)

Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system, in which, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium Streptomyces avidinii), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. (justia.com)
Both Avidin and Streptavidin possess four Biotin binding sites of very high affinity (Ka=1015 M−1). (justia.com)
Biotin has extremely high affinity, rapidity and high specificity with streptavidin or avidin. (newyorktimesnow.com)
From a biotechnological perspective, the most attractive property of biotin is its unusually high affinity and highly specific binding to avidin and streptavidin of bacterial origin. (newyorktimesnow.com)

Nucleic acids research online , 2022, str. (ki.si)

Provided herein are rational methods of affinity maturation to evolve an antibody's activity or a portion thereof based upon the structure/affinity/activity relationship of an antigen. (patentpc.com)
The affinity matured starting antibody has a higher binding specificity and affinity than the target antigen. (patentpc.com)
The method identifies a similar antibody or portion of an antibody that has a lower activity against the target antigen than its corresponding form. (patentpc.com)
A set of standard tests are defined for the performance of peptide-TCR binding, including the detection of TCRs binding to a given peptide/antigen, choosing among a set of candidate peptides for a given TCR and determining whether any pair of TCR-peptide bind. (frontiersin.org)

PNAs and their analogues are endowed with exceptionally high affinity and specificity for receptor sites, essentially due to their polyamide backbone's uncharged and flexible nature. (bvsalud.org)
The S1 subunit (PDB I.D-6VSB) of the spike protein being reported to acts as receptor binding domain (RBD), binding to ACE-2 receptor with higher affinity. (ijpsonline.com)
Moreover, peptides derived from ICAM-1 receptor can be internalized by the leukemic T-cells along with the conjugated moiety offering the promise to selectively treat cancers and autoimmune diseases. (biosyn.com)
Systematic analyses have revealed that physicochemical properties of the drug-peptide conjugates and their mechanism of receptor-mediated cellular internalization are important controlling factors for developing a successful targeting system. (biosyn.com)

Current sequencing methods allow for detailed samples of T cell receptors (TCR) repertoires. (frontiersin.org)
The affinity of a TCR for a given peptide epitope and the specificity of the binding are governed by the heterodimeric αβ T-cell receptors ( 2 ). (frontiersin.org)
Following specific binding of T cell receptors to viral and bacterial-derived peptides bound to MHC ( 5 ), or from neo-antigens ( 8 - 10 ), the appropriate T cells expand, resulting in the increased frequency of T cells carrying such receptors. (frontiersin.org)

EvoRator2: predicting site-specific amino acid substitutions based on protein structural information using deep learning Journal of Molecular Biology: 435(14):168155. (tau.ac.il)
This water-soluble vitamin is a component of five carboxylase enzymes (propionyl-CoA carboxylase, pyruvate carboxylase, methylcrotonyl-CoA carboxylase [MCC], acetyl-CoA carboxylase 1, and acetyl-CoA carboxylase Cofactor for carboxylase 2), which catalyze key steps in fatty acid, glucose, and amino acid metabolism. (newyorktimesnow.com)
This ensures that each modified antibody contains an amino acid replacement to a different amino Acid than the first. (patentpc.com)
in December 2006, UniMod lists 495 modifications including 144 amino acid substitutions [6]. (lu.se)
Nineteen amino-acid substitutions were detected between 12 iVDPVs and the parental Sabin strain, of which only one (K1419R) was found on the subsequent 10 iVDPV isolates, suggesting this site's potential as a temperature-sensitive determination site. (cdc.gov)

The polymerase chain reaction (PCR) and its variants are analytical gold standards for nucleic acid-based testing that play a critical role in diagnosing and monitoring infectious diseases. (selectbiosciences.com)
All three variants showed similar affinities to EFGR, and secondary structure contents, as the unmodified control protein. (kth.se)
Binding affinities of a peptide and one of its variants to Rb, determined by surface plasmon resonance, correlated with results from the two-hybrid assay. (dartmouth.edu)
This technology was recently used to achieve stepwise total chemical synthesis of protein chains nearing 200 amino acids in length that retained the structure and function of native variants obtained by recombinant expression. (aps2022.org)
Here, we compared CC variants using our recently improved method that measures ORF1p binding to ssDNA at different forces. (nsf.gov)

As more research labs and classrooms dive into the field of structural nucleic acid nanotechnology, students and researchers need access to interactive, dynamic, handheld models. (allenpress.com)
Building on previous research, representative nucleic acid duplexes were split into logical monomer segments, and atomic coordinates were used to create solid models for 3D printing. (allenpress.com)
These peptides (HLA-II T-cell epitopes) are increasingly important as research targets for the development of vaccines and immunotherapies. (biomedcentral.com)
As a research organization that provides full-service PNA services, Creative Peptides ' customer-centric collaborative approach helps us build true partnerships with customers. (creative-peptides.com)
Nucleic Acids Research: 51(Web Server issue):W232-W236. (tau.ac.il)
Members of the institute have published in a wide range of peer-reviewed journals, including Nature, Molecular Cell, Angewandte Chemie, JACS, Science Advances, Proceedings of the National Academy of Sciences USA, Nucleic Acids Research, Chemical Science and many others. (le.ac.uk)

The observed agonist-bound state contains the ERRgamma LBD, a ligand (GSK4716), and the RIP140 peptide and reveals an unexpected rearrangement of the phenol-binding residues. (rcsb.org)

The method also compares the amino acids sequences of the variable heavy and variable light chains of the first antibody to those of the corresponding variable heavy or variable light chains of the related antibody. (patentpc.com)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)

In fact, manufacturers have now developed many commercial nucleic acid separation kits based on magnetic beads. (aisenbio.com)

Ab-TrSOD, when compared to TrSOD, promoted a decrease in the formation of ROS of 19 and 14% at the concentrations of 1 and 6 μmol/L, respectively, indicating that this joplinks Recombinant Human Regulator of G-protein form was more effective in reducing oxidative stress compared to SOD without the cell penetrating peptide (CPP). (pimac.org)
With a value of 3x 10‐10M (Biacore™ Analysis) the affinity of the antibody is outstanding This monoclonal antibody specifically reacts with recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or at both termini. (dianova.com)
Expression of recombinant proteins in E. coli as a fusion protein with neighbouring histidine residues is one of the most popular methods, because the proteins have useful attributes. (dianova.com)

Stability and mechanism of threose nucleic acid toward acid-mediated degradation. (uci.edu)

Seven peptides were identified, allof which contain the Leu-X-Cys-X-Glu motif found in Rb-binding proteins, although their activity in the yeast assay variedover a 40-fold range. (dartmouth.edu)

This platform is currently applied for discovery of non-canonical peptide disruptors of cancer protein-protein interactions that are effective, non-toxic, long-circulating, and stable in cells and animals. (aps2022.org)
Multistep protein-protein interactions underlie most biological processes, but their characterization through methods such as isothermal titration calorimetry (ITC) is largely confined to simple models that provide little information on the intermediate, individual steps. (nsf.gov)

Both linear and cyclic peptides have been explored as trafficking moiety due to ease of synthesis, structural simplicity, and low probability of undesirable immunogenicity. (biosyn.com)
Aptamers have advantages over their other biological counterparts including high specificity and affinity, versality, reduced immunogenicity, and easy chemical modification. (neoaptamers.com)

I will discuss our results toward the discovery of high-affinity peptidomimetics that target oncoproteins or SARS-CoV-2. (aps2022.org)
New strategies for oral delivery of drug peptides and peptidomimetics. (lu.se)

Further, chemical synthesis enables the ability to rewire the covalent framework with non-natural amino acids, drugs, and carbohydrates. (aps2022.org)
I will discuss the fully automated single-shot chemical synthesis of protein chains up to 220 amino acids in hours. (aps2022.org)
In addition, rapid protein synthesis enables accelerated drug discovery when combined with our single-shot affinity-selection mass spectrometry platform. (aps2022.org)

To address the many current challenges in the field - from developing more predictive computational design methods to determining the requirements for function in cells - we combine concepts from computer science, physics, chemistry, mathematics, engineering and biology. (ucsf.edu)
The development of sequence-based predictive algorithms to identify potential Dsg3 epitopes has encountered limited success due to the paucity of PV-associated allele-specific peptides as training data. (biomedcentral.com)

Oligonucleotide which displays specific binding to a protein or other target, often selected by an iterative cycle of affinity- based enrichment. (genomicglossaries.com)
Immobilized boronic acid is designed specifically for the simple isolation of small molecular weight compounds that have cis-diol groups such as ribonucleotide and oligonucleotide RNA. (gbiosciences.com)

In silico immune evaluation using C-ImmSim server showed that the peptide could concurrently elicit cell-mediated and humoral immune responses. (ijpsonline.com)

This format requires no spotting robot and is ideal for arrays where no more than 25 probes are needed, where probe solution is scarce, or for rapid methods development. (genengnews.com)

To help fight this disease, it is necessary to establish an effective and rapid detection method. (pimac.org)
We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)

The work in this thesis shows the diverse possibilities available for changing the functionality of affinity proteins through chemical and enzymatic methods for different applications, and provides a framework for potential further improvement of both affibody and antibody functionality. (kth.se)
Using this approach, high-affinity peptidomimetic ligands were identified for the MDM2 oncogenic protein, the 12ca5 clone of anti-hemagglutinin antibody, and the signaling protein 14-3-3. (aps2022.org)
This method entails affinity maturation of a portion of a first or secondary antibody for a target protein. (patentpc.com)
This is where the target region refers to a portion of the first antibody that has at least one amino acids difference from the same area in the related antibody. (patentpc.com)
A higher number of histidine residues leads to an increased binding affinity of the antibody, e.g. an expression product with 10 histidine residues increases the affinity about 10- to 20-fold. (dianova.com)
In most cases additional flanking amino acids aren't required for antibody binding, therefore enabling the choice of many different expression vectors on the only condition that the epitope is spatially available. (dianova.com)

In peptide nucleic acid (PNA), the sugar-phosphate backbone of DNA is replaced with a protein-like backbone. (wikipedia.org)
The semi-rigid peptide nucleic acid backbone serves as a nearly-perfect template for attaching complimentary base pairs on DNA or RNA in a sequence-dependent manner as described by Watson-Crick models. (bvsalud.org)

In the structural modification of PNA, there are few types of base modification, mainly the introduction of monomers composed of pseudocytosine, in order to make the modified peptide nucleic acid and DNA form a triple helix without pH dependence. (creative-peptides.com)
Nine previously identified stimulatory Dsg3 peptides, Dsg3 96-112, Dsg3 191-205, Dsg3 206-220, Dsg3 252-266, Dsg3 342-356, Dsg3 380-394, Dsg3 763-777, Dsg3 810-824 and Dsg3 963-977, were docked into the binding groove of each model to analyze the structural aspects of allele-specific binding. (biomedcentral.com)

TFOs are short (≈15-25 nt) nucleic acid strands that bind in the major groove of double-stranded DNA to form intramolecular triplex DNA structures. (wikipedia.org)
PNAs are able to bind with high affinity and sequence specificity to a complementary DNA sequence through Watson-Crick base pairing binding and are able to form triple helices through parallel orientation Hoogsteen bonds with the PNA facing the 5'-end of the DNA strand. (wikipedia.org)
We employ new Natural Language Processing (NLP) based methods to predict whether any TCR and peptide bind. (frontiersin.org)

Wilson, D. S., Keefe, A. D. & Szostak, J. W. The use of mRNA display to select high-affinity protein-binding peptides. (nature.com)
Leveraging expertise with high-throughput identification of peptides from ultra-large combinatorial libraries, the Pentelute group has developed an affinity selection-mass spectrometry platform for rapid de novo discovery of potent binders to proteins in solution. (aps2022.org)
Methods for the automated high-fidelity chemical production of proteins are needed. (aps2022.org)
The high-affinity strong binding between biotin and avidin and the multi-level amplification effect make BAS immunolabeling and related tracer analysis more sensitive. (newyorktimesnow.com)
While both chains have been reported to be important to affect binding, we show here that for many TCR-peptide pairs the TCR's binding to target MHC-peptide can be determined with high accuracy using the β-chain only. (frontiersin.org)
In contrast, many TCR responses are characterized by a high level of cross-reactivity with single TCRs binding a large number of MHC-bound peptides, and single peptides binding a large number of TCRs ( 16 , 17 ). (frontiersin.org)
While aptamers exhibit high specificity and affinity for their intended targets, they can also exhibit non-specific binding to unintended off-targets, which can lead to undesirable effects and compromise their therapeutic potential. (neoaptamers.com)
Coupled with autosampler and fraction collector, for peptide off-line seperation, using either isocratic flow, size-exclusion chromatography, ion exchange chromatography, or Reverse phase high-pH HPLC. (carnegiescience.edu)
The Mass Distance Fin- gerprint is calculated from high accuracy measured peptide masses. (lu.se)

Two structures were obtained describing the inverse agonist-bound state, the ERRgamma LBD with 4-hydroxytamoxifen (4-OHT), and the ERRgamma LBD with 4-OHT and a peptide representing a portion of the silencing mediator of retinoid and thyroid hormone action protein (SMRT). (rcsb.org)

Applications of the Avidin-Biotin system number in the hundreds, and have recently been reviewed (Wilchek, M. and Bayer, E. A., (1990) Methods in Enzymology, 184). (justia.com)

Moreover, as the word 'coding' refers to the relationship between nucleic acids and proteins, rather than the mere transcription of DNA into RNA, it is logical to call the strand with the mRNA sequence the coding strand, as in the first example. (genomicglossaries.com)
m6A-ELISA, a simple method for quantifying N6-methyladenosine from mRNA populations. (ki.si)

Protein-Peptide Interactions Analyzed with the Yeast Two-Hybrid System" by Meijia Yang, Zining Wu et al. (dartmouth.edu)
Yang M, Wu Z, Fields S. Protein-peptide interactions analyzed with the yeast two-hybrid system. (dartmouth.edu)

These proteins show similar domain architectures as known organelle-targeted expression regulators of the octotrico peptide repeat type in algae and plants. (frontiersin.org)

The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups. (gbiosciences.com)

We collected test data from four independent studies that involved 721 peptide binding assays. (biomedcentral.com)

The American Peptide Society, APS, a nonprofit scientific and educational organization founded in 1990, provides a forum for advancing and promoting knowledge of the chemistry and biology of peptides. (aps2022.org)
This review is focused on understanding the factors involved in the development of an effective drug-peptide conjugate with an emphasis on the chemistry and biology of the conjugates. (biosyn.com)

Our findings ascertain that DRB1*0402 plays a crucial role in the selection of specific self-peptides in DR4 PV. (biomedcentral.com)

The best strategy was the identification of a single best 9-mer within the longer peptide. (biomedcentral.com)
Custom Workflow for the Confident Identification of Sulfotyrosine-Containing Peptides and Their Discrimination from Phosphopeptides. (liverpool.ac.uk)

I-FABP) and bovine heart fatty acid-binding pro- tissues and in intracellular as well as extracellular tein (H-FABP). (lu.se)

In addition, our results lend credence to the hypothesis that the alleles DQB1*0201 and *0202 play a protective role by binding Dsg3 peptides with greater affinity than the susceptible alleles, allowing for efficient deletion of autoreactive T cells. (biomedcentral.com)

Computational prediction methods complement experimental studies, minimize the number of validation experiments, and significantly speed up the epitope mapping process. (biomedcentral.com)
We tried three prediction strategies - the best 9-mer within the longer peptide, the average of best three 9-mer predictions, and the average of all 9-mer predictions within the longer peptide. (biomedcentral.com)
None of the individual predictors was shown to be suitable for prediction of promiscuous peptides. (biomedcentral.com)
We combined large-scale TCR-peptide dictionaries with deep learning methods to produce ERGO (pEptide tcR matchinG predictiOn), a highly specific and generic TCR-peptide binding predictor. (frontiersin.org)

Anatomy2

Diseases2

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes11

Information Science3

Health Care1

Molecules23

Antibodies8

Specificity and binding1

Single-stranded nucleic acid1

Oligonucleotides1

Purification9

Reagents7

Human leukocyte ant1

Molecule4

Cell-penetrat1

Proteins and peptides1

Antisense5

Antigenic peptides1

Genomic2

19951

Chromatography assay2

20201

Vivo2

Synthetic2

Gene4

Streptavidin4

20221

Antigen4

Receptor4

Receptors3

Amino acid5

Variants5

Research6

Ligand1

Sequences3

Separation1

Recombinant3

Degradation1

Assay1

Protein-protein inte2

Immunogenicity2

Peptidomimetics2

Synthesis3

Predictive2

Oligonucleotide2

Immune responses1

Probes1

Detection3

Antibody6

Backbone2

Structural2

Bind3

High9

Bound1

Biotin1

MRNA2

Interactions2

Expression1

Covalently linked1

Assays1

Chemistry and biology2

DRB11

Identification2

Intracellular1

Alleles1

Prediction4