• In this method the proteins are first separated during the first dimension electrophoresis, then instead of the diffusion towards the antibodies, the proteins are electrophoresed into an antibody-containing gel in the second dimension. (wikipedia.org)
  • Immunoprecipitation will take place during the second dimension electrophorsis and the immunoprecipitates have a characteristic bell-shape, each precipitate representing one antigen, the position of the precipitate being dependent on the amount of protein as well as the amount of specific antibody in the gel, so relative quantification can be performed. (wikipedia.org)
  • The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. (creative-biolabs.com)
  • Polyclonal antibody Goat Anti-Human Alpha-1-Acid Glycoprotein is monospecific to alpha 1 acid glycoprotein by immunoelectrophoresis and gel diffusion techniques. (leebio.com)
  • Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule horse IgG. (dianova.com)
  • Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. (dianova.com)
  • Immunoprecipitates are visible in the wet agarose gel, but are stained with protein stains like Coomassie brilliant blue in the dried gel. (wikipedia.org)
  • Third, gel suspension was prepared by mixing the MBs-BSAFITC solution with the low-gelling-temperature agarose gel (10 g/L) and filled into an electrophoresis channel. (bvsalud.org)
  • The agarose was chosen as the gel matrix because it has large pores allowing free passage and separation of proteins but provides an anchor for the immunoprecipitates of protein and specific antibodies. (wikipedia.org)
  • Fused rocket immunoelectrophoresis is a modification of one-dimensional quantitative immunoelectrophorsis used for detailed measurement of proteins in fractions from protein separation experiments. (wikipedia.org)
  • 198-2C11-1-5-1MG Quality Level 100 biological source goat conjugate peroxidase conjugate antibody form affinity isolated antibody antibody product. (gen9bio.com)
  • 15-30120 biological source: rabbit Quality Level: 100 conjugate: peroxidase conjugate antibody form: affinity purified immunoglobulin antibody product type: secondary antibodies clone: polyclonal species. (biomeda.com)
  • Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies. (wikipedia.org)
  • The agarose was chosen as the gel matrix because it has large pores allowing free passage and separation of proteins but provides an anchor for the immunoprecipitates of protein and specific antibodies. (wikipedia.org)
  • Immunoprecipitates are visible in the wet agarose gel, but are stained with protein stains like Coomassie brilliant blue in the dried gel. (wikipedia.org)
  • In contrast to SDS-gel electrophoresis, the electrophoresis in agarose allows native conditions, preserving the native structure and activities of the proteins under investigation, therefore immunoelectrophoresis allows characterization of enzyme activities and ligand binding etc. in addition to electrophoretic separation. (wikipedia.org)
  • Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. (carlova.ee)
  • Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared by pouring the warm, liquid solution into a mold. (chromnet.net)
  • Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. (gen9bio.com)
  • 519-BA1039-1 SKU/Catalog Number: BA1039 Product Description: This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific. (biomeda.com)
  • Thermo Scientific Anti-NonHuman Primate secondary antibodies are affinity-purified antibodies with well-characterized specificity for non-human primate (e.g. monkey) immunoglobulins and are useful in the detection, sorting or purification of its specified target. (gen9bio.com)
  • Thickening and gelling agent in poultry, fish and meat canning at level of 0.5 to 2.0% in the broth weight. (fao.org)
  • The principle involves the migration of charged particles through a gel medium under an electric field. (biomall.in)