ChromatographyELISAImmunogenRocket immunoelectrophoresisPolyclonalAntibody reactsAssayHigh-affinityAntibodiesProteinsCharacterizationReceptorSolid phaseHumanSpecificTechniquesRegionCrossPurificationAntibodiesAntigenSpecificityConjugateLectin affinityGoatElectrophoresisSerumHigh affinityAntigensQuantitativeFragmentsFormMethodTarget
Chromatography6
- Some of the methods are similar to affinity chromatography by use of immobilized ligands. (wikipedia.org)
- Human myeloma IgG1, λ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures. (sigmaaldrich.com)
- The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. (abcam.com)
- Biochemical separation techniques and characterization: ion exchange, size exclusion and affinity chromatography, electrophoresis, UV-visible, fluorescence and Mass spectrometry. (entrance-exam.net)
- This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594. (abcam.cn)
- The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. (creative-biolabs.com)
ELISA6
- Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. (qedbio.com)
- Identity is determined by immunoelectrophoresis and indirect ELISA. (sigmaaldrich.com)
- Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule horse IgG. (jacksonimmuno.com)
- By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. (abcam.com)
- Immunological techniques: Immunodiffusion, immunoelectrophoresis, RIA and ELISA. (entrance-exam.net)
- Ex: 495nm, Em: 519nm Host species: Goat Isotype: IgG Suitable for: ICC/IF, Flow Cyt, ELISA, IHC-P, IHC-Fr Specificity By immunoelectrophoresis and. (enigmadiagnostics.com)
Immunogen2
- Immunogen affinity purified goat IgG. (agrisera.com)
- Purity Immunogen affinity purified rabbit IgG. (agrisera.com)
Rocket immunoelectrophoresis2
- For enzymes and other ligand-binding proteins, one-dimensional electrophoresis similar to counter electrophoresis or to "rocket immunoelectrophoresis", affinity electrophoresis may be used as an alternative quantification of the protein. (wikipedia.org)
- There are several different variations of this procedure such as affinity immunoelectrophoresis, rocket immunoelectrophoresis, and fused rocket immunoelectrophoresis. (largest.org)
Polyclonal4
- Polyclonal antibody Goat Anti-Human Alpha-1-Acid Glycoprotein is monospecific to alpha 1 acid glycoprotein by immunoelectrophoresis and gel diffusion techniques. (leebio.com)
- Our Polyclonal Antibody anti-human lactoferrin forms a single precipitation arc against human lactoferrin by immunoelectrophoresis. (leebio.com)
- 15-30120 biological source: rabbit Quality Level: 100 conjugate: peroxidase conjugate antibody form: affinity purified immunoglobulin antibody product type: secondary antibodies clone: polyclonal species. (enigmadiagnostics.com)
- Bovine IgG is a preparation of purified polyclonal bovine IgG extracted frrom bovine serum, By immunoelectrophoresis, this product react with antiserum specific for bovine IgG and subclasses IgG1 and IgG2. (immunoportal.com)
Antibody reacts2
- Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG. (agrisera.com)
- Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. (agrisera.com)
Assay3
- The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. (wikipedia.org)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Goat Serum, Monkey IgM and Monkey Serum. (caslab.com)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human Transferrin and anti-Human Serum. (rockland.com)
High-affinity3
- Assays based on high-affinity detection of secretory TMKmyc levels in culture using specific antibodies are considered. (biomedcentral.com)
- Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. (rockland.com)
- This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins. (rockland.com)
Antibodies1
- Antibodies are are affinity purified using solid phase human IgG (H&L). (agrisera.com)
Proteins1
- Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins. (agrisera.com)
Characterization1
- The methods have been used for estimation of binding constants, as for instance in lectin affinity electrophoresis or characterization of molecules with specific features like glycan content or ligand binding. (wikipedia.org)
Receptor1
- These receptor molecules often take the form of affinity probes consisting of fluorophore-labeled molecules that will bind to target molecules that are mixed with the sample being tested. (wikipedia.org)
Solid phase2
- ab214879 was affinity purified using solid phase Mouse IgG. (abcam.com)
- Antibody is affinity purified using solid phase rat IgG (H&L). (agrisera.com)
Human2
- With the information gained via immunoelectrophoresis, medical professionals can better understand how the human immune system responds to toxins. (largest.org)
- The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. (rockland.com)
Specific2
- Currently, there is ongoing research in developing new ways of utilizing the knowledge already associated with affinity electrophoresis to improve its functionality and speed, as well as attempts to improve already established methods and tailor them towards performing specific tasks. (wikipedia.org)
- Affinity capillary electrophoresis (ACE) refers to a number of techniques which rely on specific and nonspecific binding interactions to facilitate separation and detection through a formulary approach in accordance with the theory of electromigration. (wikipedia.org)
Techniques1
- Affinity capillary electrophoresis can be divided into three distinct techniques: non-equilibrium electrophoresis of equilibrated sample mixtures, dynamic equilibrium ACE, and affinity-based ACE. (wikipedia.org)
Region1
- It is a glycoprotein, molecular weight approximately 70,000 that migrates in the alpha 2 region in immunoelectrophoresis. (lookformedical.com)
Cross1
- Cross electrophoresis, the first affinity electrophoresis method, was created by Nakamura et al. (wikipedia.org)
Purification2
- Anti-Llama secondary antibodies are affinity-purified antibodies with well-characterized specificity for llama immunoglobulins and are useful in the detection, sorting or purification of its specified target. (bioscience-explained.org)
- Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abcepta.com)
Antibodies6
- By utilizing specific antibodies, immunoelectrophoresis enables the identification and characterization of antigens present in a protein mixture. (microbiologynote.com)
- Immunoelectrophoresis provides insights into the interactions between antibodies and antigens. (microbiologynote.com)
- It helps in evaluating the specificity and affinity of antibodies towards their target antigens, contributing to the understanding of immune responses and immune-related disorders. (microbiologynote.com)
- Antibodies are are affinity purified using solid phase mouse IgG (H&L). (agrisera.com)
- Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. (rockland.com)
- 15-30120 biological source: rabbit Quality Level: 100 conjugate: peroxidase conjugate antibody form: affinity purified immunoglobulin antibody product type: secondary antibodies clone: polyclonal species. (biomeda.com)
Antigen4
- Immunoelectrophoresis is a biochemical approach for protein separation and characterization that is based on electrophoresis and antibody-antigen interactions. (microbiologynote.com)
- The immunoelectrophoresis procedure begins with electrophoresis, which separates the antigen mixture into its distinct components. (microbiologynote.com)
- Immunoelectrophoresis is a biochemical technique that combines electrophoresis and antibody-antigen reactions to separate and identify proteins based on their antigenic properties. (microbiologynote.com)
- Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. (dianova.com)
Specificity2
- The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP). (bioscience-explained.org)
- The specificity of anti-rabbit IgG peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. (r-web.cz)
Conjugate2
- The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). (r-web.cz)
- 198-2C11-1-5-1MG Quality Level 100 biological source goat conjugate peroxidase conjugate antibody form affinity isolated antibody antibody product. (gen9bio.com)
Lectin affinity5
- The methods have been used for estimation of binding constants, as for instance in lectin affinity electrophoresis or characterization of molecules with specific features like glycan content or ligand binding. (wikipedia.org)
- 1. Three different types of alpha-fetoprotein in the diagnosis of malignant solid tumors: use of a sensitive lectin-affinity immunoelectrophoresis. (nih.gov)
- 3. Analysis of lectin affinity immunoelectrophoretic profiles of serum alpha-fetoprotein from patients with yolk sac tumors and carcinomas of the gastrointestinal tract: correlations with molecular structures. (nih.gov)
- 7. [Analysis of lectin-affinity of alpha fetoprotein-diagnostic approach]. (nih.gov)
- 11. Serum alpha-fetoprotein subfractions in pregnant women identified by the modified method of lectin affinity crossed-line immunoelectrophoresis. (nih.gov)
Goat3
- Immunogen affinity purified goat IgG. (agrisera.com)
- Rabbit anti goat IgA antibody recognizes goat IgA and shows no cross-reactivity with other goat immunoglobulin classes in immunoelectrophoresis. (bio-rad-antibodies.com)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum, Monkey IgA and Monkey Serum. (prsbio.com)
Electrophoresis9
- Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. (wikipedia.org)
- Both qualitative and quantitative information may be obtained through affinity electrophoresis. (wikipedia.org)
- Cross electrophoresis, the first affinity electrophoresis method, was created by Nakamura et al. (wikipedia.org)
- The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. (wikipedia.org)
- Currently, there is ongoing research in developing new ways of utilizing the knowledge already associated with affinity electrophoresis to improve its functionality and speed, as well as attempts to improve already established methods and tailor them towards performing specific tasks. (wikipedia.org)
- Boronate affinity electrophoresis utilizes boronic acid infused acrylamide gels to purify NAD-RNA. (wikipedia.org)
- Affinity capillary electrophoresis (ACE) refers to a number of techniques which rely on specific and nonspecific binding interactions to facilitate separation and detection through a formulary approach in accordance with the theory of electromigration. (wikipedia.org)
- Affinity capillary electrophoresis can be divided into three distinct techniques: non-equilibrium electrophoresis of equilibrated sample mixtures, dynamic equilibrium ACE, and affinity-based ACE. (wikipedia.org)
- There are various types of protein electrophoresis techniques including native polyacrylamide gel electrophoresis (PAGE), isoelectric focusing, serum protein electrophoresis, Sodium Dodecyl sulphate electrophoresis (SDS-PAGE), zymography, affinity electrophoresis etc. (biomall.in)
Serum1
- Thus, serum protein immunoelectrophoresis should be performed in all cases in which antibody deficiency coexists with normal levels of total IgG or IgG subclass. (medscape.com)
High affinity1
- The high affinity of the lectin for its specific sugar makes it more stable in binding to the corresponding sugar-containing receptor, which is the most essential mechanism of lectin antiviral. (creative-proteomics.com)
Antigens1
- Immunoelectrophoresis can be used to estimate the concentration of specific antigens in a sample by measuring the height or intensity of precipitate arcs formed during the reaction. (microbiologynote.com)
Quantitative2
- This technique, also known as two-dimensional quantitative immunoelectrophoresis, was developed by Clarke and Freeman. (microbiologynote.com)
- This method, also known as one-dimensional quantitative immunoelectrophoresis, was introduced by Laurell. (microbiologynote.com)
Fragments1
- Nucleic acids or nucleic acid fragments may be characterized by their affinity to other molecules. (wikipedia.org)
Form1
- These receptor molecules often take the form of affinity probes consisting of fluorophore-labeled molecules that will bind to target molecules that are mixed with the sample being tested. (wikipedia.org)
Method2
- 9. Alpha-fetoprotein subfractions in amniotic fluid identified by a modification of the method of concanavalin A, lentil lectin or phytohemagglutinin-E affinity crossed-line immunoelectrophoresis. (nih.gov)
- This method, also known as one-dimensional immunoelectrophoresis, follows the approach introduced by Grabar. (microbiologynote.com)
Target1
- As affinity probes in CAE, fluorophore-labeled compounds with affinities for the target molecules are employed. (wikipedia.org)