Affinity for human cloned 5. Medical search. Frequent questions

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
In vitro stability in human serum at 37°C was analyzed using radio-thin layer chromatography and radio-high-performance liquid chromatography. (snmjournals.org)
NKp30 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. (prospecbio.com)
Purified by protein-A affinity chromatography. (lsbio.com)

Peripheral blood cells from human were stained with PE-Cy™7 Mouse Anti-Human CD16 antibody (Cat. (bdbiosciences.com)
The complex of antibody and coupled beads was detected using 50 μL of biotinylated mouse anti-human IgG (clone H2, affinity purified, Southern Biotech, Birmingham, AL, Cat. (cdc.gov)
Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). (thermofisher.com)
Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. (thermofisher.com)
Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurotensin Receptor 1, High Affinity (NTSR1) in Tissue homogenates, cell lysates and other biological fluids. (1elisakits.com)
Mouse anti Human C4d (Neoantigen) antibody, clone 057-51.5.1.6 recognizes a neoantigen on the secreted protein complement component 4d (C4d). (bio-rad-antibodies.com)
The mechanisms of action by which RTX kills FL cells include complement-dependent cytotoxicity (CDC) ( 3 ), antibody-dependent cellular cytotoxicity (ADCC) ( 4 , 5 )-herein referred to as 'effector function' mechanisms-as well as CD20 signaling effects ( 6 ). (spandidos-publications.com)
These defects include 1) reduced class switch recombination, responsible for the generation of a secondary response of class switched antibodies, 2) reduced de novo somatic hypermutation of the antibody variable region, 3) reduced binding and neutralization capacity, as well as binding specificity, of the secreted antibodies, 4) increased epigenetic modifications that are associated with lower antibody responses, 5) increased frequencies of inflammatory B cell subsets, and 6) shorter telomeres. (biomedcentral.com)
Additionally, there are different types of mAbs (murine, chimeric, humanized, human, mAbs as Antibody-drug conjugates and bispecific mAbs) discussed in the present work. (bvsalud.org)
In later experiments they developed a proprietary monoclonal antibody against human CD79 (Curly 14) that has the capacity to desensitize the BCR in vitro. (nationaljewish.org)
Further experiments will involve the characterization of the effectiveness of Curly 14 for modulating immune disease, understanding Curly 14 binding affinity, determination of the antibody binding site and the ability to destabilize and/or desensitize B cells in huSCID and human CD79 knockin mouse models. (nationaljewish.org)

We show that the DOCK homology region (DHR)-1 domain of DOCK8 binds specifically to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and is required for its recruitment to the plasma membrane. (life-science-alliance.org)

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)

The 5-HT7 receptor is a member of the GPCR superfamily of cell surface receptors and is activated by the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) The 5-HT7 receptor is coupled to Gs (stimulates the production of the intracellular signaling molecule cAMP) and is expressed in a variety of human tissues, particularly in the brain, the gastrointestinal tract, and in various blood vessels. (wikipedia.org)
Three splice variants have been identified in humans (designated h5-HT7(a), h5-HT7(b), and h5-HT7(d)), which encode receptors that differ in their carboxy terminals. (wikipedia.org)
The multiple effects of IL-5 are mediated by binding of the cytokine to specific cell surface receptors expressed on target cells. (rndsystems.com)
Cloning of cDNAs encoding the human gamma-aminobutyric acid type A receptor alpha 6 subunit and characterization of the pharmacology of alpha 6-containing receptors. (drugbank.com)
Recombinant human alpha 6 beta gamma 2S GABAA receptors have been expressed in both stably transfected cells and Xenopus oocytes, and the pharmacology of the benzodiazepine binding site has been determined. (drugbank.com)
Water soluble clozapine is a prototypic, atypical antipsychotic which binds to both serotonin and dopamine receptors (K i values are 35, 83 and 22, 250 and 141 nM at D 2 , D 3 and D 4 , D 5 , D 1 and 12.6 and 13.2 nM at 5-HT 2A and 5-HT 2C receptors respectively) and also shows activity at other receptors. (hellobio.com)
Differential regulation of rat 5-HT2A and 5-HT2C receptors after chronic treatment with clozapine, chlorpromazine and three putative atypical antipsychotic drugs. (hellobio.com)
Exhibits high affinity for 5-HT2A receptors (Ki = 0.4 nM for cloned human 5-HT2A receptors). (fishersci.com)
Also displays nanomolar affinity for human D2 receptors. (fishersci.com)
1) Both subunits form homomeric receptors with high affinity for [3H]kainate (KD = 16 and 31 nM, respectively) and L-glutamate (KD = 2 and 40 microM, respectively). (aspetjournals.org)

An essential role of 5-HT7 receptor in intestinal hyperalgesia was demonstrated in mouse models with visceral hypersensitivity, of which a novel 5-HT7 receptor antagonist administered perorally reduced intestinal pain levels. (wikipedia.org)
A recombinant human IL-5 soluble receptor alpha has been shown to bind the human IL-5 dimer in a 1:1 ratio and acts as a human -IL-5 antagonist. (rndsystems.com)

Human high-affinity Fc gamma RI (CD64) gene mapped to chromosome 1q21.2-q21.3 by fluorescence in situ hybridization. (uni-bielefeld.de)
Key evidence showing that ADCC plays an important role in the clinical effect of RTX was provided by the analysis of Fc gamma RIIIa (CD16a) polymorphisms, where patients carrying CD16a 158V/V genotype (associated with high affinity Fc gamma receptor) showed significantly improved objective response rates compared with FL patients carrying at least one 158F allele (associated with low affinity Fc gamma receptor) ( 20 ). (spandidos-publications.com)

Description: A sandwich ELISA kit for detection of Neurotensin Receptor 1, High Affinity from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (1elisakits.com)
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Neurotensin Receptor 1, High Affinity (NTSR1) in samples from tissue homogenates or other biological fluids. (1elisakits.com)
Human recombinant Intercellular Adhesion Molecule 1 was immobilized on the ELISA plates. (pku.edu.cn)
After 4 rounds affinity panning and ELISA analysis, 5 positive phage clones that can bind to ICAM-1 specifically were selected. (pku.edu.cn)

Cattle Fc gamma RII: molecular cloning and ligand specificity. (uni-bielefeld.de)
Dr. Cerundolo demonstrated how the length and saturation of lipid antigens contained within the CD1d binding site modulate their affinity of binding to invariant NKT cells (iNKT cells), hence explaining how lipid-specific lymphocytes are capable of recognizing both the group head and the length of lipid antigens, ensuring greater specificity of antigen recognition. (aacrjournals.org)

As is the case with many other cytokines, the functional high-affinity receptor for IL-5 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. (rndsystems.com)
In the case of IL-5, the beta subunit is shared with the high affinity receptor complexes for IL-3 and GM-CSF. (rndsystems.com)
A cDNA encoding the human gamma-aminobutyric acidA (GABAA) receptor alpha 6 subunit has been cloned and sequenced. (drugbank.com)
Homo sapiens protein phosphatase 5 catalytic subunit (PPP5C), transcript variant 1, mRNA. (origene.com)

cDNA clones for the alpha chain (IL-5 R alpha ) of both the mouse and human high affinity IL-5 receptor complexes have been isolated. (rndsystems.com)
In addition to the cDNA clone encoding the full-length transmembrane protein, cDNA clones that arise from alternative splicing and that encode soluble secreted forms of IL-5 R alpha have been isolated from mouse as well as human cells. (rndsystems.com)
A cDNA encoding a multispecific organic anion transporter 3 (hOAT3) was isolated from a human kidney cDNA library. (aspetjournals.org)
2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." . (wikipedia.org)

When the 5-HT7 receptor is activated by serotonin, it sets off a cascade of events starting with release of the stimulatory G protein Gs from the GPCR complex. (wikipedia.org)
Human and mouse IL-5 R alpha are both members of the hematopoietin receptor superfamily characterized by the presence of the WSXWS, and a four cysteine residue motif in the extracellular domain of the transmembrane protein. (rndsystems.com)
The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)
Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14. (thermofisher.com)
Full length human recombinant protein of human PPP5C (NP_006238) produced in HEK293T cell. (origene.com)
1997). „Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor. (wikipedia.org)
Clone G146-254 was raised against a bacterially expressed Fli-1 ets domain fusion protein. (bdbiosciences.com)
A hybridoma that produces MDI-protein specific IgM mAbs, which also has good cross-reactivity to hexamethylene diisocyanate conjugated protein, has recently been isolated and cloned. (cdc.gov)
Affinity constants for the TDI mAb are very high (108-1010 M-1) suggesting greater utility for immunoassay and immunohistochemistry, but limited use for immunoprecipitation/protein isolation. (cdc.gov)
In 1993, Lee et al cloned a human complementary DNA, rBAT (renal basic amino acid transporter) in chromosome 2, encoding a transport protein for cystine and dibasic amino acids. (medscape.com)

Metronidazole selectively radiosensitizes hypoxic cells without influencing the radiation response of normal well-oxygenated cells [5,7-9]. (who.int)
Metronidazole selectively radiosensitizes of metronidazole (in a range of therapeutic hypoxic cells without influencing the ra- concentrations) on PMN-generated free diation response of normal well-oxygenated radicals during phagocytosis activity in cells [ 5,7-9 ]. (who.int)

Agonists mimic the effects of the endogenous ligand, which is serotonin at the 5-HT7 receptor (↑cAMP). (wikipedia.org)
Naratriptan tablets are a serotonin (5-HT 1B/1D ) receptor agonist (triptan) indicated for the acute treatment of migraine with or without aura in adults. (nih.gov)
The dense granules contain proaggregatory factors such as ADP, calcium, and 5-hydroxytryptamine (serotonin). (medscape.com)

The 5-HT7 receptor is encoded by the HTR7 gene, which in humans is transcribed into 3 different splice variants. (wikipedia.org)
Conversely, rats do not express a splice variant homologous to the h5-HT7(d), as the rat 5-HT7 gene lacks the exon necessary to encode this isoform. (wikipedia.org)
A single nucleotide polymorphism in the 5'' untranslated region of the NKp30 gene has been linked to mild malaria susceptibility. (prospecbio.com)
This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. (bdbiosciences.com)
The mecA gene produces transpeptidase PBP2a (penicillin-binding peptide) that decreases the bacterial affinity for beta-lactam antibiotics. (medscape.com)

On 1 March 2021, this lineage accounted for 5% of all current sequences globally, and 100% of those identified in South Africa. (nature.com)

A 5-HT7(c) splice variant is detectable in rat tissue but is not expressed in humans. (wikipedia.org)

Anti-human NKp30 mAb, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c immunized with recombinant human NKp30 amino acids 19-138 purified from E. coli. (prospecbio.com)

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or Anti-Human CD14 Super Bright 645 (purple histogram). (thermofisher.com)

This molecule inhibits the proliferation of IL-5-dependent cell lines and blocks human umbilical cord blood eosinophil differentiation. (rndsystems.com)
Among the OAT isoforms, rOAT3, as well as rOAT1, exhibits a markedly wide substrate selectivity, and its human homolog is considered a key molecule in the renal handling of organic anions. (aspetjournals.org)

Neutral antagonists and inverse agonists are typically referred to collectively as "antagonists" and, in the case of the 5-HT7 receptor, differentiation between neutral antagonists and inverse agonists is problematic due to differing levels of inverse agonist efficacy between receptor splice variants. (wikipedia.org)
ZEMBRACE SymTouch injection contains sumatriptan succinate, a selective 5-HT1B/1D receptor agonist. (globalrph.com)
The purpose of this study was to investigate the effects of the ABCA1 agonist peptide CS-6253 on amyloid- peptides (A) and lipoproteins in plasma and cerebrospinal fluid (CSF) of cynomolgus monkeys, a species with amyloid and lipoprotein metabolism similar to humans. (cdc.gov)

In this study, two previously cloned goldfish kainate receptor subunits (GFKAR alpha and GFKAR beta) were transiently expressed in human embryonic kidney 293 cells, and their ligand-binding properties and some associated structural features were characterized, resulting in the following findings. (aspetjournals.org)

Human IL-5 is a potent eosinophil differentiation and activation factor in vivo and in vitro . (rndsystems.com)
In vitro stability in human serum was at least 95% after 4 h of incubation. (snmjournals.org)
Substitution of the two basic residues, K576 and R581, with alanine abolished PI(4,5)P2 binding in vitro, ablated the ability of DOCK8 to activate Cdc42 and support leukocyte migration in three-dimensional collagen gels. (life-science-alliance.org)
Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. (bdbiosciences.com)
2007). However, there may be limitation using in vitro data to extrapolate to an in vivo kinetics, and also from marmosets to humans by these available data alone. (who.int)

The clone g5/44 bind CD22 with subnanomolar affinity. (creativebiolabs.net)

In mammals, adenosine 3', 5'-cyclic monophosphate (cAMP) is known to play highly important roles in sperm motility and acrosomal exocytosis. (plos.org)
Cyclic AMP (adenosine 3', 5'-cyclic monophosphate) is an important player in sperm function. (plos.org)
As in somatic cells, sperm intracellular cAMP concentration is regulated by the opposite action of two enzymes: the adenylyl cyclases (AC), which synthesize cAMP from ATP, and the phosphodiesterases (PDE), which break the phosphodiester bond of cAMP to form 5'-adenosine monophosphate (AMP). (plos.org)
This initial interaction (platelet adhesion) sets the stage for other adhesive reactions that allow the platelets to interact with other agonists in the vicinity of vessel injury, such as adenosine 5'-diphosphate (ADP), subendothelial collagen, and thrombin. (medscape.com)

OAT1 is a PAH/dicarboxylate exchanger and mediates the high-affinity transport of PAH in a sodium-independent manner. (aspetjournals.org)
The high-affinity system mediates uptake of 10% of L-cystine and the dibasic amino acids at the apical membrane of the straight third segment (S3) of the proximal tubule. (medscape.com)

A naturally-occurring soluble form of the IL-5 R alpha has been detected in biological fluids of autoimmune-prone mice and mice bearing chronic B cell leukemia (BCL 1 ). (rndsystems.com)
Crystal structure of the soluble form of the human fcgamma-receptor IIb: a new member of the immunoglobulin superfamily at 1.7 A resolution. (uni-bielefeld.de)

Populations that have been reported to have increased rates of S aureus colonization include persons with type 1 diabetes, intravenous drug users, asthmatic patients, the elderly, persons who require hemodialysis or peritoneal dialysis, persons with rheumatoid arthritis, those with chronic sinusitis, and persons with human immunodeficiency virus (HIV) infection. (medscape.com)
including human immunodeficiency virus (HIV) infections and preterm birth. (bvsalud.org)

Various authors have studied amino acid transport in cell membranes obtained from the proximal renal tubule of humans, rats, and rabbits. (medscape.com)

1997;75(5):430-445. (lsbio.com)

Vincenzo Cerundolo, MD, PhD, is the director of the United Kingdom Medical Research Council (MRC UK) Human Immunology Unit and professor of immunology at the Weatherall Institute of Molecular Medicine, University of Oxford, UK. (aacrjournals.org)
Dr. Cerundolo was appointed professor of immunology at the University of Oxford in 2000, director of the MRC Human Immunology Unit in 2010, and head of the Investigative Medicine Division of the Radcliffe Department of Medicine in 2012. (aacrjournals.org)
International Immunology 5 (10): 1239-49. (wikipedia.org)

Additionally, it has also been reported that IL-5 can stimulate the proliferation and/or differentiation of basophils and B cells. (rndsystems.com)
DEHP is not classifiable as to its carcinogenicity to humans (Group 3) because peroxisome proliferation has not been documented in human hepatocyte cultures exposed to DEHP nor in the liver of exposed non-human primates. (who.int)

Dr. Cerundolo described the first human antigen processing-deficient cells, leading to the cloning and characterization of the transporter associated with antigen-processing 1 and 2 (TAP1, TAP2) genes and the identification of several families of TAP1/2-deficient patients with necrotizing granulomatous skin lesions and small vessel vasculitis. (aacrjournals.org)
2006). „Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." . (wikipedia.org)

Numerous orthosteric ligands of moderate to high affinity are known. (wikipedia.org)

1991. Physiologically based pharmacokinetic modeling with dichloromethane, its metabolite, carbon monoxide, and blood carboxyhemoglobin in rats and humans. (cdc.gov)

The recombinant receptor has a diazepam-insensitive pharmacology, with negligible affinity for a number of classic benzodiazepines. (drugbank.com)

Flow cytometric analysis of CD16 expression on human peripheral blood lymphocytes. (bdbiosciences.com)
Human peripheral blood lymphocytes. (lsbio.com)

However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. (thermofisher.com)
IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. (thermofisher.com)

120 different translocations, resulting in the generation of MLL-fusion proteins encompassing more than 75 different partner genes 5 . (nature.com)
2007). „Large-scale identification of human genes implicated in epidermal barrier function." . (wikipedia.org)

They are also able to secrete additional mediators, which are not performed by their granules such as interleukins (IL) 5 . (bvsalud.org)

How- recurrence after primary operation could be assessed for each pa- ever, recent developments in affinity proteomics have advanced tient, using her molecular portraits. (lu.se)

Thus metronidazole does not show any radiosensitizing ability when tested under aerobic conditions [5,9,12,13]. (who.int)

By itself, CD25 binds IL-2 only with low affinity. (thermofisher.com)

Interleukin 5, produced primarily by activated T cells and mast cells, has diverse biological effects on a variety of cell types. (rndsystems.com)
High and specific binding of 68 Ga-BMS-986192 to human PD-L1-expressing cancer cells was confirmed, which closely correlates with the respective PD-L1 expression level determined by flow cytometry and immunohistochemistry staining. (snmjournals.org)
Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. (thermofisher.com)
Analysis of this network shows that it recapitulates known features of the human immune system and can be used uncover novel multi-step immune pathways, examine species-specific differences in immune processes, and predict the response of immune cells to stimuli. (stanford.edu)
in turn, the malignant epithelial cells can undergo reversible epithelial-mesenchymal transitions ( 1 - 5 ). (aacrjournals.org)
Lysate from Jurkat cells was probed with anti-Fli-1 (clone G146-254) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). (bdbiosciences.com)

Because hOAT1 has been suggested to show rather limited capacity for organic anion transport, hOAT3 might play a large role in the human kidney. (aspetjournals.org)

Anatomy11

Organisms10

Chemicals and Drugs44

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes63

Information Science4

Health Care1

Chromatography4

Antibody13

Phosphatidylinositol 4,5-bisphosphate1

Purification1

Receptors10

Antagonist2

High-affinity Fc gamma2

ELISA4

Specificity2

Subunit4

CDNA4

Protein10

Normal well-oxygenated2

Serotonin3

Gene5

20211

Tissue1

Recombinant human1

Isotype1

Molecule2

Agonist3

Ligand1

Vitro5

Bind1

Adenosine4

Mediates2

Soluble form2

Immunodeficiency virus2

Amino1

19971

Immunology3

Proliferation2

Characterization2

Moderate to high1

Rats1

Pharmacology1

Peripheral blood lymph2

Gamma2

Genes2

Granules1

Molecular1

Aerobic1

Binds1

Cells7

Kidney1