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  • HPLC
  • In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated. (wikipedia.org)
  • rocket immunoelectrophoresis
  • In somewhat chronological order: Immunoelectrophoretic analysis (one-dimensional immunoelectrophoresis ad modum Grabar), crossed immunoelectrophoresis (two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ad modum Laurell), rocket-immunoelectrophoresis (one-dimensional quantitative immunoelectrophoresis ad modum Laurell), fused rocket immunoelectrophoresis ad modum Svendsen and Harboe, affinity immunoelectrophoresis ad modum Bøg-Hansen. (wikipedia.org)
  • column
  • Consider immobilized metal affinity chromatography, such as a Ni2+ column. (brainmass.com)
  • For silica column chromatography, this ratio lies within 20:1 to 100:1, depending on how close to each other the analyte components are being eluted. (wikipedia.org)
  • A faster flow rate of the eluent minimizes the time required to run a column and thereby minimizes diffusion, resulting in a better separation. (wikipedia.org)
  • Faster flow rates can be achieved by using a pump or by using compressed gas (e.g. air, nitrogen, or argon) to push the solvent through the column (flash column chromatography). (wikipedia.org)
  • An example of this situation is when trying to find an enzyme with a particular activity, where it can be possible to build an affinity column with an attached ligand that is similar or identical to the substrate of choice. (wikipedia.org)
  • In any form of chromatography, the rate at which the solute moves down the column is a direct reflection of the percentage of time the solute spends in the mobile phase. (wikipedia.org)
  • silica gel
  • For example, one of the most widely used silica gel grades in the former technique is mesh 230 - 400 (40 - 63 µm), while the latter technique typically requires mesh 70 - 230 (63 - 200 µm) silica gel. (wikipedia.org)
  • methods
  • This is not only due to its separation qualities, but also because it can be used in conjunction with a variety of other analytic methods, such as mass spectrometry, and western blotting. (wikipedia.org)
  • This methods also utilizes a separation gel made of an acrylamide-pendent Phos-Tag monomer that is copolymerized. (wikipedia.org)
  • mass spectrometry
  • We use mass spectrometry-based approaches which can do more than simply determining the identity of a protein isolated in a polyacrylamide gel -- rapidly advancing technologies are allowing us to measure dynamic changes in protein abundances, post-translational modification states, splice isoforms, interaction partners, and localization across multiple cell states. (stanford.edu)
  • The combination of liquid chromatography with tandem mass spectrometry (LC-MS/MS) has emerged as the most robust technology for making proteome-scale discoveries. (stanford.edu)
  • Eluates from gel regions equivalent to 38 kDa were analyzed by liquid chromatography-tandem mass spectrometry for protein identification. (diabetesjournals.org)
  • These trapped proteins are then visualized and identified using mass spectrometry after in-gel digestion. (wikipedia.org)
  • particle
  • As mentioned previously, particle sizes are decreasing in an attempt to achieve higher resolution and faster separations, which led to higher backpressures. (wikipedia.org)
  • characterization
  • Affinity immunoelectrophoresis has been used for estimation of binding constants, as for instance with lectins or for characterization of proteins with specific features like glycan content or ligand binding. (wikipedia.org)
  • procedure
  • Magneticaly-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. (google.com)
  • The integrated system comprises a miniaturized sample handling compartment containing a MALDI ionization surface for separating, chemically manipulating and detecting analytes by MALDI -TOF MS. The miniaturized integrated sample handling system disclosed herein is applicable to a wide range of analytical problems that require separation and/or chemical manipulation of a sample prior to mass analysis as part of the analytical procedure. (google.ca)
  • temperature
  • Natural agarose contains uncharged methyl groups and the extent of methylation is directly proportional to the gelling temperature. (wikipedia.org)
  • Synthetic methylation however have the reverse effect, whereby increased methylation lowers the gelling temperature. (wikipedia.org)
  • The separation took place under isocratic conditions using pure water as the mobile phase, and controlled the temperature using a water bath. (wikipedia.org)
  • phases
  • The separation is based on differential partitioning between the mobile and stationary phases. (wikipedia.org)
  • The stationary phases are usually finely ground powders or gels and/or are microporous for an increased surface, though in EBA a fluidized bed is used. (wikipedia.org)
  • 1940s
  • Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won the 1952 Nobel Prize in Chemistry. (wikipedia.org)
  • quantification
  • Immunoprecipitation will take place during the second dimension electrophorsis and the immunoprecipitates have a characteristic bell-shape, each precipitate representing one antigen, the position of the precipitate being dependent on the amount of protein as well as the amount of specific antibody in the gel, so relative quantification can be performed. (wikipedia.org)