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  • permeation
  • The research that appeared to spark an onslaught of modified applications was a gel permeation chromatography technique of fixing poly(isopropyl acrylate) (PIPA) strands to glass beads and separating a mixture of dextrans, which was developed by Gewehr et al. (wikipedia.org)
  • enzyme
  • An example of this situation is when trying to find an enzyme with a particular activity, where it can be possible to build an affinity column with an attached ligand that is similar or identical to the substrate of choice. (wikipedia.org)
  • silica gel
  • For example, one of the most widely used silica gel grades in the former technique is mesh 230 - 400 (40 - 63 µm), while the latter technique typically requires mesh 70 - 230 (63 - 200 µm) silica gel. (wikipedia.org)
  • column
  • The following protocol describes AC using a HiTrap ® Streptavidin HP 1 ml column by syringe, using a pump, or a chromatography system. (sigmaaldrich.com)
  • Faster flow rates can be achieved by using a pump or by using compressed gas (e.g. air, nitrogen, or argon) to push the solvent through the column (flash column chromatography). (wikipedia.org)
  • different
  • Agarose exhibits the phenomenon of thermal hysteresis in its liquid-to-gel transition, i.e. it gels and melts at different temperatures. (wikipedia.org)
  • A variety of chemically modified agaroses with different melting and gelling temperatures are available through chemical modifications. (wikipedia.org)
  • Researchers found that the main principles of Tsvet's chromatography could be applied in many different ways, resulting in the different varieties of chromatography described below. (wikipedia.org)
  • compound
  • It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. (wikipedia.org)
  • technique
  • Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won the 1952 Nobel Prize in Chemistry. (wikipedia.org)
  • available
  • The gel is also available in convenient Econo-Pac cartridges, which can be used with the Econo system, FPLC, and HLPC systems. (bio-rad.com)
  • Agarose is available as a white powder which dissolves in near-boiling water, and forms a gel when it cools. (wikipedia.org)
  • high
  • High-pressure liquid chromatography, also called high performance liquid chromatography (HPLC), is now widely used in industry. (encyclopedia.com)
  • It was in the fifties and sixties that theoretical models were developed for IC for further understanding and it was not until the seventies that continuous detectors were utilized, paving the path for the development from low-pressure to high-performance chromatography. (wikipedia.org)
  • He further showed that ATP released from the electric nerves is hydrolyzed extracellularly to adenosine that is recycled via a high affinity transport mechanism into the nerve terminals where it is rephosphorylated and taken up in the form of ATP into synaptic vesicles. (wikipedia.org)
  • constant
  • The affinity of the Pi3 for Shaker B voltage- gated potassium channels was found to be low with a dissociation constant of 140 nM.The block was reversible and not voltage dependent. (wikipedia.org)
  • analysis
  • In North America, we have a team of field application scientists and sales consultants who are focused on biomolecule characterization using microcalorimetry, light scattering, Taylor dispersion analysis, nanoparticle tracking analysis, resonant mass measurement, and size-exclusion chromatography. (materials-talks.com)
  • step
  • This reduction in positive charge interferes in the binding step of the toxin and reduces its affinity for the channel thereby signifying the importance of N-terminal sequence in channel recognition. (wikipedia.org)