Affinity cell adhesion. Medical search. Frequent questions

IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. (antibodies-online.com)
SELPLG codes for PSGL-1, the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. (wikidoc.org)
EXPERIMENTAL Techniques Components The VLA-4-particular ligand (22 -24) 4-((～12 nm) and above the for physiologically turned on VLA-4 (high affinity condition ～1-2 nm) (22). (bioinf.org)
Which means transition from the reduced affinity towards the high affinity receptor condition led to elevated binding from the probe (from ～25% to ～70-80% of receptor occupancy as computed based on the main one site binding formula) that was discovered as a rise in the indicate route fluorescence (MCF). (bioinf.org)
In this work, bicyclic Arg-Gly-Asp (RGD) peptides are established as novel selective, high-affinity integrin ligands, which can be applied for membrane-integrinvisualization and integrin-mediated cell adhesion in 2D and 3D polymer matrices. (rwth-aachen.de)
For the affinity-based screening and selection of hundreds of potential RGD-bicycles for their binding to integrins avb3, avb5 and a5b1, a fast and cost-efficient ELISA method was developed applying a high-affinity, biotinylated cysteine-knot RGD peptide(knottin-RGD) as a benchmark ligand. (rwth-aachen.de)
IC50:30-42 nM), one medium-affinity and non-selective avb5-binding peptide (CT3RGDcT3NWaCT3;IC50: 650 nM) and three high-affinity and medium-selectivity a5b1-binding peptides (CT3RGDcT3AY(D-Leu)CT3, CT3RGDcT3AWGCT3, CT3RGDcT3AYaCT3;IC50: 90-173 nM). (rwth-aachen.de)
Localization of high-affinity binding sites for oxytocin and vasopressin in the human brain. (shengsci.com)
Sites which bind oxytocin and vasopressin with high affinity were detected in the brain and upper spinal cord of 12 human subjects, using in vitro light microscopic autoradiography. (shengsci.com)
GM1 stimulates neuronal sprouting and enhances the action of nerve growth factor (NGF) by directly and tightly associating with Trk, the high-affinity tyrosine kinase-type receptor for NGF. (matreya.com)

Changing surface characteristics of scaffolds was a way to promote regeneration and ion implantation was one of the methods to modify surface properties which play a huge role in enhancing the proliferation and integration of cells. (omicsonline.org)
In this study, it was observed that the ion implantation stimulated the neural proliferation and the implantation of different ions on cell culture surfaces was essential to determine the effects of this technique on adhesion, proliferation, differentiation and apoptosis properties of cells in details. (omicsonline.org)
Hamilton: B. C. Decker Inc.). A number of anti-angiogenic activities have been reported for this protein, such as inhibition of endothelial cell proliferation, migration, and tube formation. (justia.com)
Enhances CD4+ T-cell differentiation and proliferation (PubMed:20959412). (qedbio.com)
This study seeks to clarify the influence of titanium particles on osteoblast adhesion, deformability, proliferation, and gene expression profile. (stanford.edu)
Gangliosides1 are acidic glycosphingolipids that form lipid rafts in the outer leaflet of the cell plasma membrane, especially in neuronal cells in the central nervous system.2 They participate in cellular proliferation, differentiation, adhesion, signal transduction, cell-to-cell interactions, tumorigenesis, and metastasis.3 The accumulation of gangliosides has been linked to several diseases including Tay-Sachs and Sandhoff disease. (matreya.com)
In fact hydroxyapatite, which exhibits strong similarities to the mineral phase of the mammalian bones and teeth, does play a key role during the bioactive glasses integration processes, in that it facilitates adhesion and subsequent proliferation of the osteocytes, so allowing the damaged bone tissues to be repaired. (unito.it)
Studies suggest that norrin may play a role in Wnt signaling, which is important for cell division (proliferation), attachment of cells to one another (adhesion), cell movement (migration), and many other cellular activities. (medlineplus.gov)

Lantero , S et al "Stimulation of eosinophil IgE low-affinity receptor leads to increased adhesion molecule expression and cell migration. (ersjournals.com)
Raulf-Heimsoth , M et al "T-cell receptor repertoire expression in workers with occupational asthma due to platinum salt. (ersjournals.com)
Moreover, peptides derived from ICAM-1 receptor can be internalized by the leukemic T-cells along with the conjugated moiety offering the promise to selectively treat cancers and autoimmune diseases. (biosyn.com)
To elucidate the role of affinity in regulating these processes, a technique for measuring B cell receptor affinity maturation in GCs in situ was developed. (bham.ac.uk)
For kinetic dissociation measurements without inside-out activation cell examples had been preincubated using the fluorescent probe (25 nm ～2 x (12 nm) for the relaxing condition of VLA-4 68 of receptor occupancy (22)) treated with surplus unlabeled LDV formulated with little molecule (1 μm) or substances appealing (10-30 μm) as well as the dissociation from the fluorescent molecule was implemented. (bioinf.org)
The interaction between the T cell receptor (TCR) and the antigen-presenting major histocompatibility complex (pMHC) on a contacting T cell and APC, is widely accepted to be the key interaction. (lu.se)
In the second part of the thesis, a new method to measure the two-dimensional dissociation constant (2D Kd) of ligand-receptor interactions on single cells is presented. (lu.se)
For this purpose, a SLB functionalized with TCR and an adhesion ligand, was allowed to bind cell with pMHC and the corresponding adhesion receptor. (lu.se)
GPCR 7TM website stalks are adequate receptor agonists raising the possibility for the development of adhesion GPCR synthetic peptide modulators. (biodiversityhotspot.org)
Calculations indicated, however, that CD4/pMHC II binding would increase rates of T-cell receptor (TCR) complex phosphorylation by threefold via the recruitment of Lck, with only a small, 2-20% increase in the effective affinity of the TCR for pMHC II. (lu.se)

The modules of the fibronectin monomer are organized into proteolytically resistant domains that in isolation retain their affinity for various ligands. (embl.de)

FAK's ability to bind numerous structural and signaling proteins via a variety of interactions is important for FAK activation level, and for FAK interaction with a variety of substrates localized to sites of cell adhesion. (fishersci.com)
Type I cadherin cell-adhesion proteins are similar in sequence and structure and yet are different enough to mediate highly specific cell-cell recognition phenomena. (rcsb.org)
Although focal adhesions are similar, in terms of molecular composition, to nascent adhesions and focal complexes, several adhesion proteins appear to change as focal adhesions evolve in response to tension, e.g., zyxin and vinculin . (cellmigration.org)
Podosomes are structurally divided into a core, which mainly contains proteins involved in actin polymerization (such as WASP, the Arp2/3 complex and cortactin ), and a surrounding ring populated by integrin receptors and adhesion proteins (for example, paxillin and focal adhesion kinase (FAK/Pyk2) ) [15] . (cellmigration.org)
Peptides derived from sequence of cell surface proteins, such as intercellular adhesion molecule-1 (ICAM-1), LHRH, Bombesin, and LFA-1, have shown potent binding affinity to the target cell surface receptors. (biosyn.com)
Arg-Gly-Asp (RGD) peptides,2 poly-Arg peptides), 3,4 proteins (e.g., antibodies,5 transport proteins, and transferrin6), and small molecules (e.g., folate7) have been used to selectively direct drugs to cancer cells with upregulated receptors by forming drug-carrier conjugates (Fig. 1). (biosyn.com)
The strain, which adhesion was dependent on Vn was shown to express four binding proteins in its cell wall. (lu.se)
Of these four proteins, from 16, 21, 38 and 52 kDa molecular weights, the 52 kDa protein had the highest affinity for Vn. (lu.se)
Many proteins in adhesion plaque such as integrin, cytoskeleton proteins, proteases, protein kinases, phosphatases take part in signal transferring by co-localizing with vinculin and talin [ 3 ]. (omicsonline.org)
In many T cell activation studies, the SLB contains the nickel-chelating lipid DGS-NTA(Ni) to functionalize the SLB with histidine-tagged proteins. (lu.se)
This property has likely an implication in the adsorption processes at the hydrated layer interface and on the affinity of such materials towards proteins. (unito.it)
Indeed, if reactive Ca-OH groups were present at the surface, denaturation of proteins would occur hampering cells adhesion. (unito.it)
Ricin causes toxicity by inhibiting the formation (synthesis) of proteins in the cells of the exposed individual. (cdc.gov)

Scaling down the size and increasing the throughput of glycosyltransferase assays: activity changes on stem cell differentiation. (buffalo.edu)
Objective: To characterize pulp stem cells and evaluate their capacity for expansion and differentiation in vitro. (bvsalud.org)
Results: It was observed that the pulp stem cells exhibited the capacity to adhere to plastic and a high rate of expansion and, after detection with specific stains, it was shown that the cells were capable of differentiation into osteoblasts and chondroblasts, but not into adipocytes. (bvsalud.org)
Stem cells are cells with a low degree of differentiation that have the capacity to reproduce and can generate differentiated cells of several different types of tissues 1 . (bvsalud.org)
The objective of this study is to isolate and characterize stem cells from the pulp of permanent third molars and analyze the cells obtained in terms of their morphology and chondrogenic, osteogenic and adipogenic cell differentiation. (bvsalud.org)

For example, cell adhesion to a fibronectin substratum involves concurrent activation of Src and phosphorylation of the FAK activation loop. (fishersci.com)
Agonist activity at alpha5beta1 in human K562 cells assessed as increase in cell adhesion to fibronectin pre-incubated for 30 mins before fibronectin. (bindingdb.org)
Agonist activity at alpha5beta1 integrin in human K562 cells assessed as increase of fibronectin-mediated cell adhesion after 30 mins by fluorescence. (bindingdb.org)
Fibronectin is a multi-domain glycoprotein, found in a soluble form in plasma, and in an insoluble form in loose connective tissue and basement membranes, that binds cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. (embl.de)
Fibronectin is an extracellular matrix glycoprotein that plays a role in a number of physiological processes involving cell adhesion and migration. (embl.de)
Previous studies have shown that small beads coated with FN7-10, a four-domain cell adhesion fragment of fibronectin, bind to cell surfaces and translocate rearward. (biologists.com)
Role of endothelial cell-substrate contact area and fibronectin-recept" by Jeffrey S. Burmeister, Valerie Z. McKinney et al. (pacific.edu)

Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. (fishersci.com)
Rho activation is thought to generate focal adhesions and actin bundling due to increased myosin II activity. (cellmigration.org)
Owing to their highly localized nature, cell-matrix adhesions were initially called focal adhesions. (cellmigration.org)
Like nascent adhesions, they also tend to either disassemble (turnover) or grow and elongate into focal adhesions. (cellmigration.org)
Focal adhesions: this term is currently reserved for mature adhesions that evolve slowly over time [12] . (cellmigration.org)
Although their molecular composition is very similar to that of focal adhesions, their spatial distribution is radial, forming dot-like structures similar to 'suction cups' [14] . (cellmigration.org)
Whereas the formation of focal adhesions requires actomyosin-mediated contractile force mediated by RhoA , podosome formation is stimulated by a local loss of contractility and the recruitment of negative regulators of RhoA, such as p190RhoGAP [16] . (cellmigration.org)

Integrins are transmembrane receptors that mediate cell adhesion by forming links between the extracellular matrix and cytoskeleton. (biologists.com)

Thus, we conclude that one mechanism by which DHEA may exert its protection in animal models is via the differential regulation of adhesion molecule expression. (biomedcentral.com)
Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). (qedbio.com)

Heparin binding inhibits cell binding and cell adhesion functions of the protein. (wikipedia.org)
Since the protein has been implicated in these processes it is believed editing might effect apoptosis, regulation of cell growth and angiogenesis. (wikipedia.org)
The LAP [leucine-rich and postsynaptic density-95/Discs large/zona occludens-1 (PDZ)] protein erbin and δ-catenin, a component of the cadherin-catenin cell adhesion complex, are highly expressed in neurons and associate through PDZ-mediated interaction, but have incompletely characterized neuronal functions. (jneurosci.org)
This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. (antibodies-online.com)
The latter represents a conformationally modified form of a normal cellular PrP C , which is a normal host protein found on the surface of many cells, in particular neurons. (medscape.com)
These findings highlight the remarkable ability of evolution to exploit a wide range of molecular properties to produce closely related members of the same protein family that have affinity differences finely tuned to mediate their biological roles. (rcsb.org)
No other protein was shown to mediate staphylococcal adhesion in this thesis. (lu.se)
Purification of the antigen (endothelial cell adhesion molecule) from rat lung extracts revealed a protein with a 110-kD mol wt. (rupress.org)
Full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell. (origene.com)
Members of this protein family have an affinity for beta-galactosides. (origene.com)
This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. (origene.com)
Anti-human ESAM was purified by affinity chromatography with immobilized Protein A. (reliatech.de)
This indicates that TCR-pMHC binding in cell contacts depends significantly on the local environment and not only on the protein-protein interaction per se. (lu.se)
It is recommended to add 0.1 mL of RIPA Lysis Buffer to each well of a 6-well plates (the protein content in different cells may vary, and the volume of the lysate added can be appropriately adjusted). (elabscience.com)
Adhesion G protein-coupled receptors (GPCRs) regulate cells development and malignancy progression. (biodiversityhotspot.org)
NDP gene mutations that cause this condition affect the ability of the norrin protein to bind with frizzled-4, interfering with the specialization of retinal cells for their unique sensory function. (medlineplus.gov)

It can mediate adhesion to the shunt surface and it can also block the inhibitory effect of Vn on the complement system. (lu.se)

After two, four and 24 hours, fluorescence activated cell sorter (FACS) analysis for vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and E-selectin was performed. (biomedcentral.com)

IGFBP7 binds to IGF with low affinity compared to IGFBPs 1-6. (wikipedia.org)
P-selectin glycoprotein ligand-1 ( PSGL-1 ) is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P stands for platelet ), which is one of a family of selectins that includes E-selectin (endothelial) and L-selectin (leukocyte). (wikidoc.org)
PSGL-1 can bind to all three members of the family but binds best (with the highest affinity) to P-selectin. (wikidoc.org)
The NH2-terminal domain of rat CD2 binds rat CD48 with a low affinity and binding does not require glycosylation of CD2. (ox.ac.uk)
These results show that rat CD48 binds CD2 on its NH2-terminal IgSF domain with a low affinity and that binding is independent of glycosylation. (ox.ac.uk)

1997). "Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin" . (wikidoc.org)
Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. (reliatech.de)
Metabolic regulation of UDP-GlcNAc supply to Golgi N-glycan remodeling regulates glycoprotein affinities for galectins -and in turn, trafficking and presentation at the cell surface. (gr.jp)

The first step in this interaction process is carried out by PSGL-1 interacting with P-selectin and/or E-selectin on endothelial cells and adherent platelets. (wikidoc.org)
This interaction results in "rolling" of the white blood cell on the endothelial cell surface followed by stable adhesion and transmigration of the white blood cell into the inflamed tissue. (wikidoc.org)
Cell adhesion (the physical interaction of a cell with another cell or with the extracellular matrix (ECM)) is essential for cell migration and tissue integrity. (cellmigration.org)
On the other hand, the heparin binding properties of endostatin manifest themselves in its interaction with the cell surface. (justia.com)
The galectin lattice is a multi-valent interaction of galectins with glycoproteins at the cell surface that displays rapid exchange of binding partners with properties of liquid-liquid phase transitions, thereby acting as an intermediary between freely diffusing glycoproteins and stable complexes in the membrane. (gr.jp)
If the interaction is favourable, then T cell activation occurs. (lu.se)
A large pool of research has been aimed at characterizing this interaction by measuring the binding kinetics and relating it to the T cell response. (lu.se)
In the final part of the thesis, the interaction of TCR-pMHC in the presence of adhesion molecules of different length and density is studied. (lu.se)
Adhesion molecule pairs of similar height as TCR-pMHC have been argued to facilitate the TCR-pMHC interaction by physically keeping the opposing membranes at an optimal distance for binding. (lu.se)
Furthermore, even for height-matched adhesion molecules the 2D Kd of the TCR-pMHC interaction was found to be dependent on the relative density fraction of TCR to adhesion molecules, with low fractions of TCR molecules giving 2-3 times weaker binding. (lu.se)
Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). (qedbio.com)
The upper limit of the affinity of the rat CD48-CD2 interaction is 4 x 10(5) M-1, lower than the published affinity of human CD2 for CD58. (ox.ac.uk)
In this regard, the former alteration affects the affinity of platelet-VWF interaction. (buffalo.edu)
Whereas binding was undetectable in solution, the affinity of the CD4/pMHC II interaction could be measured in 2D using CD4- and adhesion molecule-functionalized, supported lipid bilayers, yielding a 2 D Kd of ∼5,000 molecules/μm 2 . (lu.se)
This initial interaction (platelet adhesion) sets the stage for other adhesive reactions that allow the platelets to interact with other agonists in the vicinity of vessel injury, such as adenosine 5'-diphosphate (ADP), subendothelial collagen, and thrombin. (medscape.com)

Briefly, IgSFs are adhesion molecules that bind to themselves (homophilic) or compatible IgSFs (heterophilic) across cell-cell junctions. (elifesciences.org)
It has previously been shown that small differences in the homophilic and heterophilic binding affinities of different type I family members can account for the differential cell-sorting behavior. (rcsb.org)
The extracellular region contains one V type and one C2 type Ig domain and is involved in homophilic adhesion. (reliatech.de)

Subsequently, the developed AFM technique was applied in interrogation of B cells and hybridomas - expressing receptors of varying affinity towards NP - and two GCs in tissue sections (Chapter 6). (bham.ac.uk)

Fluorescence microscopy showed that the trimer and pentamer bound strongly to the cell surface, and within 5 minutes were prominently localized to actin fiber bundles. (biologists.com)
fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the "Focal Adhesion Targeting" sequence (FAT). (fishersci.com)
To date, many strategies have been investigated to accomplish this goal, and some of these strategies rely on the differences between the cellular compositions of the targeted cells and those of the nontargeted cells. (biosyn.com)
Furthermore, the differences in cellular trafficking profiles and the pH of endosomes between normal and cancer cells have also been exploited for selective drug delivery to a specific compartment in the intracellular space of cancer vs. normal cells. (biosyn.com)
The differences in cell adhesion affinities lead to cellular interference in people with PCDH19-related epilepsies. (epilepsygenetics.net)
Analysis of the cellular phenotype showed that the cells were negative for CD45, CD69, CD117 and HLADR, and positive for CD13, CD44, CD73, CD90 and CD105. (bvsalud.org)

It also stimulates cell adhesion. (wikipedia.org)

Cell-cell adhesion maintains epithelial tissues, supports functional contacts between specialized cells, and can facilitate directed migration (for example, radial glia can guide neuronal progenitor cells to specific layers of the brain). (cellmigration.org)
Cell-matrix adhesion is the best-studied form of adhesion that mediates cell migration, and is the focus of this outline. (cellmigration.org)
Endostatin inhibits endothelial cell migration by inhibiting phosphorylation of focal adhesion kinase via binding to α5β1 integrin (Wickstrom et al. (justia.com)
At infection sites, activated T cells secrete cytokines (eg, interferon-gamma [IFN-gamma]) that induce production of macrophage migration inhibitory factor, preventing macrophages from leaving. (msdmanuals.com)

von Willebrand factor (VWF) propeptide binding to VWF D'D3 domain attenuates platelet activation and adhesion. (buffalo.edu)

implying that this complex has functional roles that extend beyond its ability to regulate cell adhesion. (jneurosci.org)
The Abl-related gene (Arg) requires its F-actin-microtubule cross-linking activity to regulate lamellipodial dynamics during fibroblast adhesion. (nih.gov)

As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. (wikidoc.org)
ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cellto cell junctions. (reliatech.de)
In particular, we are interested in applying quantitative experimental and computational techniques to discover new mechanisms that control the cell adhesion properties of blood leukocytes and platelets during human inflammatory and thrombotic disorders. (buffalo.edu)
The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
The body's reaction to vessel wall injury is rapid adhesion of platelets to the subendothelium. (medscape.com)

These results indicate that vesicles obtained from in situ perfused organs are a convenient immunogen for the production of antibodies to compartment-specific endothelial cell surface molecules, and reinforce the concept that endothelial cell surface components are selectively recognized by circulating cancer cells during metastasis formation. (rupress.org)

Anti-Vn antibodies could furthermore block the adhesion of the mentioned strain, but not of other strains tested. (lu.se)
In article Plasmon‐Enhanced Biosensing: Plasmon‐Enhanced Biosensing for Multiplexed Profiling of Extracellular Vesicles Hyungsoon Im and co‐workers label extracellular vesicles, which are nanoscale phospholipid vesicles secreted by cells, with fluorescently conjugated antibodies. (harvard.edu)
sCD48-CD4-coated polystyrene Dynabeads formed rosettes on rat CD2-transfected COS-7 cells, and this rosetting was blocked by anti-CD2 (OX34) and anti-CD48 (OX45) monoclonal antibodies. (ox.ac.uk)

Scheme depicts the process of adhesion maturation. (cellmigration.org)
Focal complexes are adhesions in the early stages of maturation. (cellmigration.org)
Fibrillar adhesions represent an extreme in the maturation of adhesions and are not generally seen in migrating cells. (cellmigration.org)

Attachment of circulating tumor cells to endothelial cell adhesion molecules restricted to select vascular compartments is thought to be responsible for site-specific metastasis. (rupress.org)
Depletion of Arg/Abl2 improves endothelial cell adhesion and prevents vascular leak during inflammation. (nih.gov)

The major cell types within the interfacial granulomatous fibrous tissues consist of fibroblasts, macrophages, lymphocytes, and foreign-body giant cells. (stanford.edu)
Dendritic cells are present in the skin (as Langerhans cells), lymph nodes, and tissues throughout the body. (msdmanuals.com)
Macrophages are phagocytic cells present in tissues throughout the body. (msdmanuals.com)
Norrin participates in chemical signaling pathways that affect the way cells and tissues develop. (medlineplus.gov)

J Cell Biol (1993) 121 (6): 1423-1432. (rupress.org)
2002) J Cell Biol, 158: 529-539). (justia.com)
J Cell Biol, 2019 Dec 2. (nih.gov)
J Cell Biol, 2004 May 10. (nih.gov)

Interactions between immune cells such as T cells and antigen-presenting cells (APCs) are integral for mounting an adaptive immune response. (lu.se)
The αβ T-cell coreceptor CD4 enhances immune responses more than 1 million-fold in some assays, and yet the affinity of CD4 for its ligand, peptide-major histocompatibility class II (pMHC II) on antigen-presenting cells, is so weak that it was previously unquantifiable. (lu.se)
Although some antigens (Ags) can stimulate the immune response directly, T cell-dependent acquired immune responses typically require antigen-presenting cells (APCs) to present antigen-derived peptides within major histocompatibility complex (MHC) molecules. (msdmanuals.com)

Gα subunits were purified using the coexpressed GST-Ric-8 affinity purification method (23). (biodiversityhotspot.org)

These links are used in cell motility as the actin cytoskeleton generates force pulling the integrin and attached matrix molecules towards the cell center. (biologists.com)
On the ventral surface this rearward movement of the cytoskeleton contributes to forward motion of the cell relative to the substrate. (biologists.com)

Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

These differences could be in the expressed surface receptors (i.e., the absence or presence of certain receptors), the metabolism profiles (i.e., different enzyme expression or intracellular trafficking), the site/location of the cells (i.e., circulating in blood stream vs. organ), and the nature of the cells (i.e., normal vs. cancerous). (biosyn.com)
These differences can be used to discriminate the delivery of a drug to diseased cells rather than to normal cells. (biosyn.com)
This is measured on individual cell-SLB contacts, providing an accurate new means of measuring binding affinity and to study differences in the 2D Kd in the cell population. (lu.se)

However, inflammation causes the expression of cell adhesion molecules (CAM) such as P-selectin on the surface of the blood vessel wall. (wikidoc.org)
Many of the projects in my laboratory assess the roles of fluid/hydrodynamic forces, biochemical reaction networks and cell adhesion molecule binding properties on blood cell adhesion mechanics during inflammation, thrombosis & hemostasis, and cancer. (buffalo.edu)
Our studies of inflammation biology examine the contribution of cell surface carbohydrate structures or glycans to white blood cell (leukocyte) activation and adhesion. (buffalo.edu)

The galectin lattice (i) regulates flow of receptors and solute transporters to coated-pit endocytosis and/or caveolin domains, and (ii) promotes turnover of cell-cell contacts such as immune synapses and focal adhesion complexes. (gr.jp)
they have receptors for the crystallizable fragment (Fc) region of immunoglobulin (Ig) G and for complement, which enable them to bind with immune complexes and present the complex to B cells in germinal centers of secondary lymphoid organs. (msdmanuals.com)

J Cell Sci (2002) 115 (12): 2581-2590. (biologists.com)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Thus, FAK activity is regulated by a complex set of phosphorylation sites, and thisphospho-regulation could be important for cell motility, cell growth, cytoskeletal organization, and adhesion-dependent cell survival. (fishersci.com)
The affinity of CD4/pMHC II therefore seems to be set at a value that increases T-cell sensitivity by enhancing phosphorylation, without compromising ligand discrimination. (lu.se)

Here we use a combination of X-ray crystallography, analytical ultracentrifugation, surface plasmon resonance and double electron-electron resonance (DEER) electron paramagnetic resonance spectroscopy to identify the molecular determinants of type I cadherin dimerization affinities. (rcsb.org)
Because substantial interclonal variance exists in relative virulence, molecular population genetic studies have led to the concept that the unit of bacterial pathogenicity is the clone or cell line. (cdc.gov)

In the brains of male mice completely lacking PCDH19, neurons sorted appropriately because all cells exhibited the same adhesion affinities. (epilepsygenetics.net)

CD2, CD48 and CD58 are structurally similar cell adhesion-molecules forming a subset of the immunoglobulin superfamily (IgSF). (ox.ac.uk)

Lung-metastatic rat R3230AC-MET breast and RPC-2 prostate carcinoma cells bound outside-out endothelial cell membrane vesicles, prepared by perfusion of the rat lung vasculature with a low-strength formaldehyde solution, in significantly higher numbers than their nonmetastatic counterparts R3230AC-LR and RPC-LR. (rupress.org)
Focal attachments have 10-15 nm distance between the cell membrane and the material surface and they are tight connections. (omicsonline.org)

However, adhesion pairs of different height than that of TCR-pMHC are also important for cell-cell contact formation and have been shown to result in an impaired T cell response if removed. (lu.se)
To better understand how, and if, adhesion molecules of different lengths influences TCR-pMHC binding the 2D Kd of TCR-pMHC in the presence of differently-sized adhesion molecules was studied. (lu.se)
In addition, the TCR-pMHC distributed non-homogeneously in the cell-SLB contacts when having height-mismatched adhesion molecules, but homogeneously when having height-matched adhesion molecules. (lu.se)
Here, we report that a soluble form of CD4 failed to bind detectably to pMHC II in surface plasmon resonance-based assays, establishing a new upper limit for the solution affinity at 2.5 mM. (lu.se)
However, when presented multivalently on magnetic beads, soluble CD4 bound pMHC II-expressing B cells, confirming that it is active and allowing mapping of the native coreceptor binding site on pMHC II. (lu.se)
Whereas binding was undetectable in solution, the affinity of the CD4/pMHC II. (lu.se)

Dendritic cells in the skin act as sentinel APCs, taking up antigen, then traveling to local lymph nodes where they can activate T cells. (msdmanuals.com)
Follicular dendritic cells are a distinct lineage (a different cell type than conventional dendritic cells), do not express class II MHC molecules, and therefore do not present antigen to Th cells. (msdmanuals.com)

Small changes in sequence are found to produce subtle structural and dynamical changes that impact relative affinities, in part via electrostatic and hydrophobic interactions, and in part through entropic effects because of increased conformational heterogeneity in the bound states as revealed by DEER distance mapping in the dimers. (rcsb.org)
The ability to measure over long time scales opens the possibility to study systems with strong affinity at the single-molecule limit, such as lectin-carbohydrate interactions responsible for cell recognition and adhesion," Hafner said. (nanowerk.com)
Pancreatic cancer spatial transcriptomics The lethality and treatment-refractory nature of pancreatic cancer are largely mediated by collaborative interactions between cancer cells and other cell types in the tumor microenvironment, including cancer-associated fibroblasts and immune cells. (harvard.edu)
In the first part of this thesis I show that interactions between DGS-NTA(Ni) and the T cells can lead to, unwanted, T cell signaling. (lu.se)
The DGS-NTA(Ni) signaling was argued to be due to TCR-DGS-NTA(Ni) interactions and stressed the importance of adequately blocking these interactions in T cell activation studies. (lu.se)

For example, tumor cells have certain upregulated receptors, enzymes, and other metabolic features that are not present in normal cells in the body. (biosyn.com)

Podosomes are ring-shaped adhesions often found in fast-moving cells, such as macrophages. (cellmigration.org)

Arg/Abl2 modulates the affinity and stoichiometry of binding of cortactin to F-actin. (nih.gov)

1- Affinity phase which occurs very fast. (omicsonline.org)
This development occurs under the influence of macrophage colony-stimulating factor (M-CSF), which is secreted by various cell types (eg, endothelial cells, fibroblasts). (msdmanuals.com)

Intracellular antigens (eg, viruses) can be processed and presented to CD8 cytotoxic T cells by any nucleated cell because all nucleated cells express class I MHC molecules. (msdmanuals.com)
Extracellular antigens (eg, from many bacteria) that are phagocytosed or endocytosed can be processed into peptides and complexed with surface class II MHC molecules on professional APCs, which specialize in presenting antigens to CD4 helper T (Th) cells. (msdmanuals.com)

Nascent adhesions are the first observable adhesive structures, emerging within the lamellipodium. (cellmigration.org)
Protocadherins, including PCDH19, are adhesive molecules sticking out from the cell surface and can be conceptualized as the tiny hooks and loops of a strip of Velcro. (epilepsygenetics.net)
Cells with similar adhesive properties are sorted together. (epilepsygenetics.net)
Pathogenic variants in PCDH19 result in loss of adhesive function, which alters the types of cells the cell adheres to and the way it will be sorted during brain development. (epilepsygenetics.net)
The cells expressing wildtype PCDH19 had normal adhesive properties, whereas the cells without PCDH19 had altered adhesive properties. (epilepsygenetics.net)

Parmentier , M et al "Regulation of lipopolysaccharide-mediated interleukin-1beta release by N-acetylcysteine in THP-1 cells. (ersjournals.com)
He completed my graduate training in Dr. Michael Demetriou's lab studying the regulation of N-glycan branching in T cells, and its dysregulation in multiple sclerosis. (gr.jp)

Activated by cytokines and chemokines, leukocytes and endothelial cells express distinct adhesion molecules on their cell surfaces [ 9 ]. (biomedcentral.com)
Sokullu E, Dağcı T, Gözen O, Ersoy F, Öztarhan A (2017) The Effects of Carbon and Gold Ion Implanted Surfaces on Neuronal Stem Cells' Functions. (omicsonline.org)
Both avb3- and a5b1-selective bicycles promoted HUVEC adhesion and growth on 2D ELR surfaces with much higher efficiencies than linear benchmark GRGDS, and with at least equal or even higher efficiencies as compared to mono-cyclic (cyclo-[KRGDf]) and knottin-RGD benchmark peptides. (rwth-aachen.de)

These adhesions are usually physically linked to large, readily visualized actomyosin bundles [13] , and their appearance correlates inversely with motility, such that they are conspicuously absent in cells migrating within a three-dimensional (3D) substrate. (cellmigration.org)

Immunofluorescence staining with a mixture of primary human dermal lymphatic endothelial cells (HDLEC) / normal human dermal fibroblasts (NHDF) with anti-human ESAM (green) [Cat# 102-42AG] and anti-human CD31 (red) [Cat# 101-M92]. (reliatech.de)

Reinmuth , N et al "Ploidy, expression of erbB1, erbB2, P53 and amplification of erbB1, erbB2 and erbB3 in non-small cell lung cancer. (ersjournals.com)

RGC feature selectivity arises from their synapses with a specific subset of amacrine (AC) and bipolar cell (BC) types, but how RGC dendrites arborize and collect input from these specific subsets remains poorly understood. (elifesciences.org)

In mice it seems to be an immune factor regulating T-cell checkpoints , and it could be a target for future checkpoint inhibitor anti-cancer drugs. (wikidoc.org)
This effect goes along with reduced amounts of infiltrating immune cells in organs such as lung and liver. (biomedcentral.com)
Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)
Here we comprehensively characterized the kinetics of cell-mediated and humoral immune responses in VRP-vaccinated mice, and demonstrate that they predominantly target the nucleoprotein (NP). (cdc.gov)

2- Adhesion phase takes longer and makes a stronger attachment. (omicsonline.org)

Peter Krutzik, Ph.D., a senior scientist in Gary Nolan's group at the Baxter Lab in genetics, pharmacology, microbiology, and immunology at Stanford University, described the use of phosphor flow cytometry to profile perturbations in signaling networks when peripheral blood cells are exposed to drug compounds. (genengnews.com)
The overall results of this work reveal that bicyclic RGD peptides represent an entirely novel and valuable platform with high potential for the development of new cell integrin biomarkers as well as cell adhesion-promoting compounds. (rwth-aachen.de)

Nearly 50 different mouse retinal ganglion cell (RGC) types sample the visual scene for distinct features. (elifesciences.org)
Pederick and colleagues discovered that the brains of female mice who only had one functional copy of PCDH19 - equivalent to people with PCDH19 -related epilepsy - showed distinct cell populations that were unable to adhere properly to one another. (epilepsygenetics.net)

Anatomy40

Organisms10

Diseases5

Chemicals and Drugs122

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes51

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

High affinity10

Proliferation8

Receptor10

Ligands1

Proteins13

Differentiation5

Fibronectin7

Focal adhesions7

Integrins1

Molecule expression2

Protein16

Mediate1

Intercellular1

Binds5

Glycoprotein3

Interaction15

Homophilic3

Varying affinity towards1

Fluorescence2

Cellular5

Stimulates1

Migration4

Platelet1

Regulate2

Platelets6

Endothelial cell surface1

Antibodies3

Maturation3

Vascular2

Tissues4

Biol4

Antigen-prese3

Purification1

Cytoskeleton2

Localization1

Differences3

Inflammation3

Complexes2

20022

Phosphorylation2

Molecular2

Neurons1

Immunoglobulin superfamily1

Membrane2

PMHC6

Dendritic2

Interactions5

Tumor cells1

Macrophages1

Modulates1

Occurs2

Antigens2

Adhesive5

Regulation2

Surfaces3

Substrate1

Fibroblasts1

ErbB21

Synapses1

Immune4

Attachment1

Compounds2

Distinct2